Labshake search
Citations for abcam :
2351 - 2400 of 2500 citations for 6 Methyl 5 propyl 4 1H pyrimidinone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Cells were washed in PBS for 5 min before being blocked with 10% normal donkey serum (Abcam, ab7475, Lot GR3234297-32) diluted in PBS for 20 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Fixed cells were blocked with 5% BSA in PBS for 1 hr at room temperature and incubated with rabbit anti-mouse Ki67 primary antibody (Abcam ab15580) at 1:200 dilution overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissue samples were washed with PBS three times for 5 minutes each before adding a secondary goat anti-rabbit IgG H & L (1:1000, Alexa Fluor® 555, Abcam) and Goat Anti-Rat IgG H & L (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were sectioned in the sagittal plane to a thickness of 5 μm prior to immunoperoxidase staining for Nestin-1 (Abcam, ab237036), a marker that is used to detect vessels in AOM-induced tumors41,42.
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bioRxiv - Neuroscience 2022Quote: ... in a heating chamber (37°C) in which the choroid plexus was loaded with Fluo-8 AM (5 μM, 30 min, Abcam, ab142773) with subsequent continuous wash with HEPES-aCSF (~1 ml/min ...
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bioRxiv - Immunology 2020Quote: ... The transferrin levels in cells were tested by Western blotting using anti-transferrin antibody (1:5□000 dilution, ab82411, Abcam, USA) as described above ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were then washed with TBST buffer (5 × 1 min) and probed with secondary rabbit anti-rat antibodies conjugated with peroxidase (1:1000, Abcam #ab6734). Blots were washed as described above and detection was carried out using Amersham ECL Western Blotting Select Detection Reagent (GE Lifesciences #RPN2235) ...
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bioRxiv - Neuroscience 2022Quote: ... spinal transverse sections 5 mm caudal and rostral from the lesion center were prepared and stained for BDA or PKCγ (Abcam, ab109539). For mice with uPx ...
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bioRxiv - Cell Biology 2022Quote: ... deparaffinized sections were rehydrated and stained using specific antibodies against Leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5, ab219107, Abcam, USA), Lysozyme (ab76784 ...
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bioRxiv - Genomics 2021Quote: ... were used to capture 400 μL of SUMO-2/3 antibody (8A2, acquired from Abcam, ab81371; ∼5-10 μg/μL antibody). All washing and handling steps were followed by centrifugation of the beads at 500g for 3 min in a swing-out centrifuge with delayed deceleration and careful aspiration of buffers ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... which was blocked with 5% (w/v) skimmed milk powder in PBS and probed for ERα at a concentration of 1:1000 (Abcam ab108398) and PRβ at a concentration of 1:1000 (Abcam ab46535 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were blocked with 5% BSA in PBS for 30 min at room temperature and incubated with anti-Rad51(abcam ab133534) primary antibody at a 1:500 dilution in 1% BSA for 90 min at room temperature or 24 hr at 4°C and was followed by incubation with Alexa Fluor 488 goat anti-rabbit secondary antibody (Invitrogen A11008 ...
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bioRxiv - Neuroscience 2022Quote: ... and 20 μM ThT in the presence or absence of purified recombinant NRN1 (5 μg/mL or 263 nM; Abcam, ab69755) protein ...
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bioRxiv - Neuroscience 2022Quote: ... The rats received bilateral lesions in the LHb with ibotenic acid (5 µg/µl in 0.01 M PBS, Abcam, Cambridge, UK). The coordinates for the LHb (AP −3.3 mm ...
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bioRxiv - Cell Biology 2024Quote: ... membranes were re-stained with rabbit monoclonal anti-SMAD1/5 (SMAD1: 1:500, ab33902; SMAD5: 1:1,000, ab40771; both Abcam, Berlin, Germany) or rabbit monoclonal anti-SMAD2/3 (1:250 ...
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bioRxiv - Immunology 2023Quote: ... 5% non-fat skim milk) and subsequently incubated with a rabbit anti-HIV-1 p55+p24+p17 antibody (Abcam, 1:2000) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... then the brain slides incubated in a secondary antibody for 90 min protected from light at room temperature (0.1% TritonX-100, 5% normal goat serum, PBS solution with 1:500 goat anti-rabbit Alexa 488; Abcam, Cambridge, MA). Slides were washed again as described above ...
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bioRxiv - Neuroscience 2024Quote: ... Sections measuring 5 μm were then cut using the Leica RM2165 and subsequently stained with Movat’s Pentachrome staining kit (Abcam Cat# ab245884). For H&E staining analysis ...
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bioRxiv - Neuroscience 2024Quote: ... The tissues were washed three times with PBS following fixation and blocked with blocking buffer: 5% normal goat serum (NGS, Abcam, AB7681) in PBS with 0.1% Triton-X (PBST) ...
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bioRxiv - Microbiology 2023Quote: ... 5 s at 60% amplitude) in 320 µL of buffer A containing 2 % (v/v) NP-40 (Abcam, Cat. No. ab142227). The aggregated proteins were sedimented at 15,000 x g for 30 min at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... washed further 5 times with PBS pH 7.4 and incubated for one hour with Goat anti-monkey (Rhesus macaque) (Abcam cat # a112767), diluted 1:2000 in PBS pH 7.4 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... paraffin-embedded and 5 µm-thick sections were stained with hematoxylin and eosin (H&E) or picrosirius red solution (Abcam #ab150681) as in [18] ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed 3 times with PBS for 5 minutes and incubated with AlexaFluor® 647 conjugated secondary antibodies (Ab150079, Ab150115; Abcam) for 1 hour at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... pH 7.5) three times, incubated in blocking solution (1% [w/v] bovine serum albumin, 1% [w/v] normal goat serum (Abcam ab7481) in TBS with 0.2% [v/v] Tween-20 [TBST]) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cultures were then washed 5x 5 minutes each and incubated with Alexa594-conjugated Goat α-Mouse IgG H&L (Abcam, #ab150116) diluted 1:500 in wash buffer for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... and next incubated for 1 hour in a solution of PB with 5% normal goat serum (Abcam Cat# ab7481, RRID: AB_2716553). Subsequently ...
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bioRxiv - Genomics 2023Quote: ... 0.1% Tween 20) and incubated over night with the desired antibody diluted in 5% milk / TBST [1:1000 H3 (abcam ab1791); 1:1000 Val1744 cleaved NICD1 (Cell Signaling Technology 4147)] ...
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bioRxiv - Neuroscience 2024Quote: ... brains and proboscis were washed 5 times for 10 minutes with PB + 0.3% Triton X- 100 (PBT) and blocked for 1 hour in PBT + 5% normal goad serum (NGS) (Abcam, ref. ab7481). Later ...
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bioRxiv - Microbiology 2024Quote: ... using a rabbit anti-β-tubulin at 1:500 dilution in 5% milk + TBS-T and 0.02% sodium azide (abcam, ab15568) combined with an anti-rabbit-HRP in a dilution of 1:4000 (GE Healthcare ...
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bioRxiv - Immunology 2024Quote: ... Cells were blocked with blocking buffer (5% goat/mouse serum in 0.3% triton-x100 in 1xPBS) and stained with rabbit anti-human CD3ζ (abcam cat# Ab226263). Coverslips were then gently washed 3 time with 1x PBS for 10 minutes each and stained with Donkey anti- Mouse IgG Antibody ...
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bioRxiv - Cancer Biology 2024Quote: ... ready to use DISCOVERY OmniMap anti-Rb HRP (760-4311) for PHOX2B and Phospho-SMAD-1/5/9 or rabbit anti-mouse secondary (Abcam, ab133469) followed by DISCOVERY OmniMap anti-Rb HRP (760-4311 ...
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bioRxiv - Physiology 2024Quote: ... Frozen brains were sliced into 7um and incubated in 0.1%PBST + 5%BSA with anti-Iba1 antibody (diluted 1:300, Abcam; Cat#ab178846) and/or anti-GFP antibody (diluted 1:200 ...
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bioRxiv - Microbiology 2024Quote: ... The cells were then treated with 5% BSA in PBS for 90 minutes at 25°C followed by treatment with anti-Listeria antibody (Abcam: ab35132) at 25°C for 2 hours ...
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bioRxiv - Physiology 2024Quote: ... incubated with blocking buffer (PBS + 5%FCS +0,1%Tween) for 1 hour and stained with anti-Iba1 antibody (diluted 1:300, Abcam; Cat#ab178846) and /or anti-TOM20 antibody (diluted 1:1000 ...
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bioRxiv - Immunology 2024Quote: ... Slides were washed 3 x 5 min and stained for 10 min with 1:50 DAB Chromogen in DAB substrate (Abcam ab64238). Slides were rinsed 4x ...
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bioRxiv - Neuroscience 2024Quote: ... 800 µl of chromatin was mixed with 3200 µl of chromatin dilution buffer and 5 µg of rabbit polyclonal anti-V5 (Abcam, ab15828) or normal rabbit IgG (Merk ...
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bioRxiv - Cancer Biology 2024Quote: ... for nuclear counterstaining for 5 min at room temperature and the slides were mounted with Anti-Fade Fluorescence Mounting Medium (Abcam, ab104135) before examination under an AxioScan7 microscope (Zeiss ...
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bioRxiv - Genetics 2024Quote: ... The next day the blot was washed 3 times for 5 minutes in PBST before adding 1:500 dilution of anti-Ret antibody (Abcam: ab134100) in PBST and with overnight shaking at 4°C ...
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bioRxiv - Genomics 2020Quote: ... and immunoprecipitation was performed using 4 μg of the appropriate antibody (anti-CTCF: 61311, Active Motif; anti-RAD21: ab88572, Abcam; anti-GFP: ab290, Abcam). Paired-end sequencing was performed on a NovaSeq6000 platform (Illumina ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... then incubated overnight at 4°C with the following primary antibodies in 2% w/v BSA: Rabbit anti-Gclc antibody (Abcam, United Kingdom; 1:1000); rabbit anti-NQO1 antibody (Cell Signalling ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated with the primary antibodies of interested proteins at 4°C overnight (Nestin, 1:1000, Sigma; Tuj1, 1:1000, Sigma; GFAP, 1:1000, Abcam; Ki67, 1:1000, ProteinTech). The next day ...
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bioRxiv - Neuroscience 2020Quote: ... mice were fixed with 4% paraformaldehyde and the brain was sectioned at 200 μm thickness and immunostained against GFP (Abcam, no. ab13970, 1:300). Confocal microscopy (Zeiss LSM 700 confocal microscope ...
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bioRxiv - Physiology 2021Quote: ... Pre-synaptic motoneuron terminals were then labeled by incubating bundles overnight at 4°C in the same blocking solution to which had been added a mouse anti-synaptophysin antibody (AB8049 Abcam, USA; 1:25 dilution). The following morning bundles were washed (5 x 30 min ...
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bioRxiv - Physiology 2022Quote: ... 0,05% Tween 20 (TBS-t) for 60 min at RT and incubated overnight at 4°C with anti-IL6 antibody (Abcam, ab229381, 1:1000 dilution). Following 3 washes (10 min ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were then treated with 3% bovine serum albumin in PBS and incubated overnight at 4°C with the primary mouse antibody anti-Pax8 (Abcam ab53490, diluted 1/100). After washing unbound antibodies ...
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bioRxiv - Microbiology 2020Quote: ... before probing overnight at 4°C with antibodies raised to the following targets: goat polyclonal antibody to influenza A virus (ab20841, Abcam Inc., Toronto, ON, Canada), Mouse anti influenza A NA 1 (GeneTex ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight at 4◻C in varying mixtures of the following primary antibodies: rabbit anti-DARPP-32 (1:1000; Abcam Cat# ab40801, RRID:AB_731843), mouse anti-Hu (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody incubations were performed for 24 hours at 4°C using an anti-glial fibrillary acidic protein (GFAP; Abcam, ab53554; 1:250 dilution) antibody ...
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bioRxiv - Microbiology 2020Quote: ... The rest of the chromatin was incubated overnight at 4°C with 1 μg of polyclonal anti-GFP antibody (Abcam ChIP grade, Abcam 290) or ...
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bioRxiv - Immunology 2022Quote: ... The membranes were blocked with 5% nonfat dry milk at room temperature for 2 hours and then incubated at 4°C overnight with primary antibodies against β-tubulin (Abcam, Cambridge, MA, USA), PD-L1 (ABclonal ...