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Citations for PacBio :
51 - 100 of 1293 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... DNA samples were prepared for PacBio SMRT-Bell sequencing by following the standard PacBio sequencing protocol (https://www.pacb.com/wp-content/uploads/Procedure-Checklist-Preparing-SMRTbell-Libraries-using-PacBio-Barcoded-Overhang-Adapters-for-Multiplexing-Amplicons.pdf) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The library was bound to polymerase using the Sequel II Binding Kit 2.0 (PacBio) and loaded onto PacBio Sequel II) ...
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bioRxiv - Genomics 2024Quote: ... The sample was eluted with 120 μl of elution buffer (PacBio Ref. No. 101-633-500). When DNA was transferred at any time throughout the extraction progress ...
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bioRxiv - Cancer Biology 2024Quote: ... Subsequent library preparation was performed with the Express Template Kit 2.0 (PacBio).
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bioRxiv - Developmental Biology 2024Quote: ... we built and assessed the quality of the CLT from PacBio HiFi reads following our previous pipeline31 ...
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bioRxiv - Developmental Biology 2024Quote: ... and the other half subjected to amplification of the lineage barcode followed by PacBio Sequel-based HiFi sequencing of the lineage barcode (Figure 1A).
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Neutralizing gut-derived lipopolysaccharide as a novel therapeutic strategy for severe leptospirosisbioRxiv - Microbiology 2024Quote: ... Sequencing libraries were generated using the SMRTbellTM Template Prep Kit (PacBio) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2024Quote: ... lucifugus fibroblast line was isolated using the Nanobind ®HMW DNA extraction kit for cultured cells (PacBio). M ...
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bioRxiv - Genomics 2024Quote: ... (0.75% DF Marker S1 high pass 6-10kb v3) and DNA was further purified with PacBio AMPure beads and treated with DNA damage repair mix (PacBio) (37°C for 60 mins ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We thus validated our haplotype block analysis by estimating the correspondence between our allele frequency-based inferred haplotypes and (1) sequences from PacBio HiFi-based long read assemblies of the 12 PSGs in five diploid and five tetraploid samples of A ...
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bioRxiv - Genomics 2024Quote: ... DNA samples for Oxford Nanopore or PacBio long read sequencing were enriched for HMW DNA (> 25 kb) employing the Short Read Eliminator Kit (Circulomics/PacBio). Quality control was performed by determining A260/A280 and A260/A230 ratios on NanoDrop ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We sheared HMW gDNA to a target DNA size distribution between 15 – 20 kb and concentrated it using 0.45X of AMPure PB beads (PacBio; Cat. #100-265-900). We performed the enzymatic incubations as follows ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Real-Time (SMRT) library using SMRTbell Express Template Prep Kit v2.0 (PacBio, CA; Cat. #100-938-900). We sheared HMW gDNA to a target DNA size distribution between 15 – 20 kb and concentrated it using 0.45X of AMPure PB beads (PacBio ...
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bioRxiv - Genomics 2024Quote: ... A DNA damage repair step was performed using the « SMRTbell Damage Repair Kit SPV3 » (PacBio). A total of 9.4μg of DNA was purified and then sheared at 20 kb using the Megaruptor system (Diagenode) ...
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bioRxiv - Genomics 2024Quote: ... The final preparation of the library was performed using the Sequel® II binding kit 3.2 (PacBio Ref. No. 102-194-100). In brief ...
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bioRxiv - Genomics 2024Quote: ... which were purified with SMRTbell® cleanup beads (PacBio Ref. No. 102158-300). The quantity and fragment size of the SMRTbell library were inspected with Qubit® Fluorometer and overnight pulse-field gel electrophoresis ...
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bioRxiv - Genomics 2024Quote: ... The final DNA sample was eluted with 120 μl of elution buffer (PacBio Ref. No. 101-633-500) and was subjected to quality check using the NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer ...
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bioRxiv - Genomics 2024Quote: ... a SMRT bell library was constructed using the SMRTbell® prep kit 3.0 (PacBio Ref. No. 102-141-700), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Parental strain PS-216 genome was previously sequenced using an Illumina MiSeq platform (genome sequence available in the NCBI database under BioSample accession number SAMN08637096) [22] and additionally sequenced by Pacific Biosciences RSII platform (PacBio) using 10 kbp single-molecule real-time (SMRTbell ...
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bioRxiv - Microbiology 2024Quote: ... followed by a cleaning step using the Nanobind CBB kit (PacBio, USA). The quality and concentration of extracted DNA were determined using a Qubit 4 fluorometer with dsDNA broad range (BR ...
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bioRxiv - Microbiology 2024Quote: ... 1 µL of 10 µM 3’ nucleotide tagging primer (PacBio_3pri_C or PacBio_3pri_G), 20 µL KOD Hot Start Master Mix ...
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bioRxiv - Microbiology 2024Quote: ... 2 µL of 10 µM 5’ PacBio round 2 forward primer (PacBio_5pri_RND2), 2 µL of 10 µM 3’ PacBio round 2 reverse primer (PacBio_3pri_RND2) ...
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bioRxiv - Microbiology 2024Quote: ... 1 µL of 10 µM 3’ nucleotide tagging primer (PacBio_3pri_C or PacBio_3pri_G), 20 µL KOD Hot Start Master Mix ...
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bioRxiv - Microbiology 2024Quote: ... 1 µL of 10 µM 5’ nucleotide tagging primer (PacBio_5pri_G or PacBio_5pri_C), 1 µL of 10 µM 3’ nucleotide tagging primer (PacBio_3pri_C or PacBio_3pri_G) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The library was bound to polymerase using the Sequel II Binding Kit 2.0 (PacBio). Sequencing was performed on PacBio Sequel II 8M SMRT cells.
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bioRxiv - Genomics 2024Quote: ... following the manufacturer’s recommended protocol (PacBio Protocol #102-166-600 REV02) with the following modifications ...
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bioRxiv - Genomics 2024Quote: Iso-seq data were processed using the command-line tools from PacBio’s SMRT Link software (available at https://www.pacb.com/) ...
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bioRxiv - Genomics 2024Quote: ... a pipeline for mitochondrial genome assembly from PacBio HiFi reads (or the assembled contigs/scaffolds) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Oxford Nanopore Technology (ONT) and Pacific Biosciences (PacBio) reads were quality-checked using the NanoPack tool (De Coster et al ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the 20-kb insert size SMRTbell library was constructed and sequenced on the Sequel II platform (PacBio, Menlo Park, CA) at Berry Genomics (Beijing).
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bioRxiv - Evolutionary Biology 2024Quote: ... HMW gDNA for long-read sequencing was extracted using the Nanobind tissue kit (PacBio, Menlo Park, CA, USA) following the application note for isolation of HMW DNA from frozen tilapia testis tissue.
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bioRxiv - Genomics 2024Quote: ... using kinetic features from PacBio CCS reads ...
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bioRxiv - Genomics 2024Quote: ... The sample was eventually eluted with 120 μl of elution buffer (PacBio Ref. No. 101-633-500) and stored at 4 °C ...
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bioRxiv - Genomics 2024Quote: A SMRTbell library was made using the SMRTbell® prep kit 3.0 (PacBio Ref. No. 102-141-700), following the provided protocol ...
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bioRxiv - Genomics 2024Quote: ... The SMRTbell library was then purified with SMRTbell® cleanup beads (PacBio Ref. No. 102158-300). The size and concentration of the library were assessed with the pulse-field gel electrophoresis and the Qubit® Fluorometer and Qubit™ dsDNA HS and BR Assay Kits (Invitrogen™ Cat ...
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bioRxiv - Genomics 2024Quote: ... connected to the software SMRT Link v11.0 (PacBio). HiFi reads are eventually generated and collected for further analysis ...
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bioRxiv - Genomics 2024Quote: ... The Sequel® II binding kit 3.2 (PacBio Ref. No. 102-194-100) was used for final preparation ...
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bioRxiv - Genomics 2024Quote: ... Length distributions observed by PacBio sequencing at the DMPK locus following TALEN or TALENRECODED expression were compared using the Student t-test (Figure 4) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and SMRTbell cleanup beads (PacBio). For 9 samples ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 5µg of DNA was purified then repaired using “SMRTbell Damage Repair Kit SPV3” (PacBio) and sheared between 15kb to 20 kb using the Megaruptor 1 system (Diagenode) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Purification steps were performed using AMPure PB beads (PacBio) and SMRTbell cleanup beads (PacBio) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... whole DNA from this protist was sequenced using both long (PacBio) and short (Illumina ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and barcoded using barcoded overhang adapters 8A/B (PacBio) and nuclease treated with SMRTbell enzyme cleanup kit 1.0 (PacBio) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Each sample underwent AMPure PB bead (PacBio, CA, USA) purification and concentration before undergoing library preparation using the SMRTbell Express Template Prep Kit 2.0 (PacBio ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The sequencing chemistry used was Sequel II Sequencing Plate 2.0 (PacBio) and the Instrument Control Software v10.1.0.119549 or 10.1.0.125432 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and nuclease treated with SMRTbell enzyme cleanup kit 1.0 (PacBio). The resulting libraries were quantified by fluorescence (Invitrogen Qubit 3.0 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Binding of the library pools to the sequencing polymerase was completed using Sequel II Binding Kit v2.0 or 2.2 (PacBio). Calculations for primer to template and polymerase to template binding ratios were kept at default values ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Each pool was sequenced on 1-2 Sequel II SMRTcells 8M (PacBio) on the Sequel IIe instrument ...
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bioRxiv - Evolutionary Biology 2024Quote: ... purification and concentration before undergoing library preparation using the SMRTbell Express Template Prep Kit 2.0 (PacBio) and barcoded using barcoded overhang adapters 8A/B (PacBio ...