Labshake search
Citations for PacBio :
51 - 100 of 1293 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... The sequencing chemistry used was Sequel II Sequencing Plate 2.0 (PacBio) and the Instrument Control Software v10.1.0.119549 or 10.1.0.125432 ...
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bioRxiv - Genomics 2024Quote: HiFi reads from PacBio Sequel II were assembled with Hifiasm (Cheng et al ...
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bioRxiv - Genomics 2024Quote: ... Purification steps were performed using AMPure PB beads (PacBio) and SMRTbell cleanup beads (PacBio) ...
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bioRxiv - Genomics 2024Quote: ... A DNA damage repair step was performed using the « SMRTbell Damage Repair Kit SPV3 » (PacBio). A total of 9.4μg of DNA was purified and then sheared at 20 kb using the Megaruptor system (Diagenode) ...
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bioRxiv - Genomics 2024Quote: ... Size selection was performed with Pippin HT using a high-pass cutoff of 17 kbp (Sage Science, HTP0001 & HPE7510.) Libraries were sequenced on the Sequel II platform on SMRT Cells 8M (PacBio, 101-389-001) using Sequel II Sequencing Chemistry 3.2 (PacBio,102-333-300 ...
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bioRxiv - Genomics 2024Quote: ... Further selection of high molecular weight DNA was performed using the Short Read Eliminator (SRE) kit (PacBio) according to the manufacturer’s guide ...
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bioRxiv - Genomics 2024Quote: Extracted DNA was size selected using the SRE XS kit (Pacbio US, Catalogue number: SKU 102-208-200) following manufacturer’s instructions to remove fragments < 10 kb in length.
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bioRxiv - Genomics 2024Quote: ... The libraries were purified using AMPure PB beads (PACBIO) and size selected using BluePippin (Sage science) ...
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bioRxiv - Genomics 2024Quote: ... the DNA went through a size selection step using a SRE Kit (PacBio, SKU-102-208-300) to remove fragments up to 25kb ...
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bioRxiv - Genomics 2024Quote: ... DNA was extracted using either the Nanobind Tissue Big DNA kit (cell line and brain) or the Nanobind HT 1ml blood kit (blood) (PacBio). For the cell line and blood samples ...
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bioRxiv - Genomics 2024Quote: ... The resulting cDNA was used as input for the MAS-Seq for 10x Single Cell 3’ kit by PacBio.
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bioRxiv - Genomics 2024Quote: ... and SMRTbell cleanup beads (PacBio). A DNA damage repair step was performed using the « SMRTbell Damage Repair Kit SPV3 » (PacBio) ...
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bioRxiv - Genomics 2024Quote: ... using Sequel II Sequencing Chemistry 3.2 (PacBio,102-333-300) with 2-hour pre-extension and 30-hour movies ...
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bioRxiv - Genomics 2024Quote: ... The extracted HMW gDNA was purified using high-salt-phenol-chloroform (PacBio) to remove coprecipitating contaminants ...
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bioRxiv - Genomics 2024Quote: ... using settings 28/31 and used to generate PacBio HiFi libraries via the SMRTbell Prep Kit 3.0 (PacBio, 102-182-700). Size selection was performed with Pippin HT using a high-pass cutoff of 17 kbp (Sage Science ...
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bioRxiv - Genomics 2024Quote: ... The final preparation of the library was performed using the Sequel® II binding kit 3.2 (PacBio Ref. No. 102-194-100). In brief ...
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bioRxiv - Genomics 2024Quote: ... which were purified with SMRTbell® cleanup beads (PacBio Ref. No. 102158-300). The quantity and fragment size of the SMRTbell library were inspected with Qubit® Fluorometer and overnight pulse-field gel electrophoresis ...
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bioRxiv - Genomics 2024Quote: ... The final DNA sample was eluted with 120 μl of elution buffer (PacBio Ref. No. 101-633-500) and was subjected to quality check using the NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer ...
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bioRxiv - Genomics 2024Quote: ... a SMRT bell library was constructed using the SMRTbell® prep kit 3.0 (PacBio Ref. No. 102-141-700), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... then prepared a Pacific Biosciences (PacBio) library and sequenced it on 3 SMRT Cells (Sequel IIe system).
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bioRxiv - Genomics 2024Quote: ... The sequenced data were managed with SMRT Analysis software suite (PacBio, Version: release_6.0.0.47841) and resulted in a set of full-length transcripts.
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bioRxiv - Genomics 2024Quote: ... PacBio WGS HiFi reads were downloaded from HGSVC2 (http://ftp.1000genomes.ebi.ac.uk/vol1/ftp/data_collections/HGSVC2/working/20191005_YRI_PacBio_NA1924 0_HiFi/). Illumina WGS (30X coverage ...
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bioRxiv - Cell Biology 2024Quote: ... lucifugus fibroblast line was isolated using the Nanobind ®HMW DNA extraction kit for cultured cells (PacBio). M ...
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bioRxiv - Genomics 2024Quote: A SMRTbell library was then constructed with the SMRTbell® prep kit 3.0 (PacBio Ref. No. 102-141-700), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: The Sequel® II binding kit 3.2 (PacBio Ref. No. 102-194-100) was used for final preparation of sequencing ...
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bioRxiv - Genomics 2024Quote: ... A SMRTbell library was then prepared by following the protocol of the SMRTbell® prep kit 3.0 (PacBio Ref. No. 102-141-700). Briefly ...
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bioRxiv - Genomics 2024Quote: ... Ligation of T-overhang SMRTbell adapters was then performed and the SMRTbell library was purified with SMRTbell® cleanup beads (PacBio Ref. No. 102158-300). The concentration and size of the library were examined with the Qubit® Fluorometer and Qubit™ dsDNA HS and BR Assay Kits (Invitrogen™ Cat ...
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bioRxiv - Genomics 2024Quote: ... The movie was captured by the software SMRT Link v11.0 (PacBio) and HiFi reads are generated and collected for further analysis ...
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bioRxiv - Genomics 2024Quote: ... The DNA sample was eluted in 80 µL elution buffer (PacBio Ref. No. 101-633-500) and its quantity and quality was assessed with NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer ...
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bioRxiv - Genomics 2024Quote: A final library preparation was performed with The Sequel® II binding kit 3.2 (PacBio Ref. No. 102-194-100) before sequencing ...
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bioRxiv - Genomics 2024Quote: ... The sheared DNA sample was purified using SMRTbell® cleanup beads (PacBio Ref. No. 102158-300), from which 2 µL of sample was taken for quality check through overnight pulse-field gel electrophoresis and Qubit® Fluorometer quantification ...
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bioRxiv - Genomics 2024Quote: ... The sample was then eventually eluted with 120 μl of elution buffer (PacBio Ref. No. 101-633-500). Throughout the extraction progress ...
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bioRxiv - Microbiology 2024Quote: ... followed by a cleaning step using the Nanobind CBB kit (PacBio, USA). The quality and concentration of extracted DNA were determined using a Qubit 4 fluorometer with dsDNA broad range (BR ...
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bioRxiv - Microbiology 2024Quote: ... 1 µL of 10 µM 3’ nucleotide tagging primer (PacBio_3pri_C or PacBio_3pri_G), 20 µL KOD Hot Start Master Mix ...
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bioRxiv - Microbiology 2024Quote: ... 2 µL of 10 µM 5’ PacBio round 2 forward primer (PacBio_5pri_RND2), 2 µL of 10 µM 3’ PacBio round 2 reverse primer (PacBio_3pri_RND2) ...
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bioRxiv - Microbiology 2024Quote: ... 1 µL of 10 µM 3’ nucleotide tagging primer (PacBio_3pri_C or PacBio_3pri_G), 20 µL KOD Hot Start Master Mix ...
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bioRxiv - Microbiology 2024Quote: ... 1 µL of 10 µM 5’ nucleotide tagging primer (PacBio_5pri_G or PacBio_5pri_C), 1 µL of 10 µM 3’ nucleotide tagging primer (PacBio_3pri_C or PacBio_3pri_G) ...
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Neutralizing gut-derived lipopolysaccharide as a novel therapeutic strategy for severe leptospirosisbioRxiv - Microbiology 2024Quote: ... Sequencing libraries were generated using the SMRTbellTM Template Prep Kit (PacBio) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... as implemented in the SMRT Portal (PacBio). The resulting genome was polished three times using Quiver (PacBio ...
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bioRxiv - Molecular Biology 2024Quote: ... 2014) was used to create a new structural annotation integrating data from de novo transcriptomes (based on Illumina and PacBio reads obtained in this study), ab initio gene prediction and database gene models ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Samples were prepared for PacBio sequencing (PacBio Sequel SMRTcell, 20h, v3 chemistry) and were sequenced at the MIT BioMicroCenter ...
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bioRxiv - Immunology 2024Quote: ... the 16S rRNA gene amplicons were generated with the barcoded Full-Length 16S rRNA gene primers (forward: AGRGTTYGATYMTGGCTCAG, reverse: RGYTACCTTGTTACGACTT) from PacBio and the 2x Roche KAPA HiFi Hot Start Ready Mix ...
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bioRxiv - Microbiology 2024Quote: The index isolate DA14734 was sequenced with long-read whole genome sequencing using the Pacific Biosciences platform (PacBio, Menlo Park, CA). DNA was prepared from overnight cultures using the Qiagen Genomic-Tip 100 ...
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bioRxiv - Genomics 2024Quote: ... (0.75% DF Marker S1 high pass 6-10kb v3) and DNA was further purified with PacBio AMPure beads and treated with DNA damage repair mix (PacBio) (37°C for 60 mins ...
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bioRxiv - Genomics 2024Quote: ... A SMRTbell library was constructed using the SMRTbell® prep kit 3.0 (PacBio Ref. No. 102-141-700), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... a SMRTbell library was constructed using the SMRTbell® prep kit 3.0 (PacBio Ref. No. 102-141-700), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... followed by DNA purification with SMRTbell® cleanup beads (PacBio Ref. No. 102158-300). 2 µL sheared DNA was used to perform overnight pulse-field gel electrophoresis while the remaining sheared DNA was stored in a 2 mL DNA LoBind® Tube (Eppendorf Cat ...
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bioRxiv - Genomics 2024Quote: ... The Sequel® II binding kit 3.2 (PacBio Ref. No. 102-194-100) was used for final preparation for sequencing ...
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bioRxiv - Genomics 2024Quote: ... The resulting DNA was eluted with 80 µL elution buffer (PacBio Ref. No. 101-633-500) and was subject to quality check using the NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer ...
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bioRxiv - Genomics 2024Quote: ... the SMRTbell library was purified with SMRTbell® cleanup beads (PacBio Ref. No. 102158-300). The concentration and size of the library were examined using the Qubit® Fluorometer and Qubit™ dsDNA HS and BR Assay Kits (Invitrogen™ Cat ...