Labshake search
Citations for PacBio :
101 - 150 of 1293 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Evolutionary Biology 2024Quote: ... The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer’s recommended protocol ...
-
bioRxiv - Genomics 2024Quote: ... The purified linearized library was used to prepare a PacBio sequencing library using a SMRTbell prep kit 3.0 (PacBio #102-141-700), following the manufacturer’s recommended protocol (PacBio Protocol #102-166-600 REV02 ...
-
bioRxiv - Genomics 2024Quote: ... the DNA went through a size selection step using a SRE Kit (PacBio, SKU-102-208-300) to remove fragments up to 25kb ...
-
bioRxiv - Genomics 2024Quote: ... DNA was extracted using either the Nanobind Tissue Big DNA kit (cell line and brain) or the Nanobind HT 1ml blood kit (blood) (PacBio). For the cell line and blood samples ...
-
bioRxiv - Genomics 2024Quote: ... The resulting cDNA was used as input for the MAS-Seq for 10x Single Cell 3’ kit by PacBio.
-
bioRxiv - Genomics 2024Quote: ... and SMRTbell cleanup beads (PacBio). A DNA damage repair step was performed using the « SMRTbell Damage Repair Kit SPV3 » (PacBio) ...
-
bioRxiv - Genomics 2024Quote: ... using Sequel II Sequencing Chemistry 3.2 (PacBio,102-333-300) with 2-hour pre-extension and 30-hour movies ...
-
bioRxiv - Genomics 2024Quote: ... The extracted HMW gDNA was purified using high-salt-phenol-chloroform (PacBio) to remove coprecipitating contaminants ...
-
bioRxiv - Genomics 2024Quote: ... using settings 28/31 and used to generate PacBio HiFi libraries via the SMRTbell Prep Kit 3.0 (PacBio, 102-182-700). Size selection was performed with Pippin HT using a high-pass cutoff of 17 kbp (Sage Science ...
-
bioRxiv - Genomics 2024Quote: HiFi reads from PacBio Sequel II were assembled with Hifiasm (Cheng et al ...
-
bioRxiv - Genomics 2024Quote: ... Purification steps were performed using AMPure PB beads (PacBio) and SMRTbell cleanup beads (PacBio) ...
-
bioRxiv - Genomics 2024Quote: ... Size selection was performed with Pippin HT using a high-pass cutoff of 17 kbp (Sage Science, HTP0001 & HPE7510.) Libraries were sequenced on the Sequel II platform on SMRT Cells 8M (PacBio, 101-389-001) using Sequel II Sequencing Chemistry 3.2 (PacBio,102-333-300 ...
-
bioRxiv - Genomics 2024Quote: ... Further selection of high molecular weight DNA was performed using the Short Read Eliminator (SRE) kit (PacBio) according to the manufacturer’s guide ...
-
bioRxiv - Genomics 2024Quote: ... The libraries were purified using AMPure PB beads (PACBIO) and size selected using BluePippin (Sage science) ...
-
bioRxiv - Genomics 2024Quote: ... then prepared a Pacific Biosciences (PacBio) library and sequenced it on 3 SMRT Cells (Sequel IIe system).
-
bioRxiv - Genomics 2024Quote: Extracted DNA was size selected using the SRE XS kit (Pacbio US, Catalogue number: SKU 102-208-200) following manufacturer’s instructions to remove fragments < 10 kb in length.
-
bioRxiv - Genomics 2024Quote: ... The sequenced data were managed with SMRT Analysis software suite (PacBio, Version: release_6.0.0.47841) and resulted in a set of full-length transcripts.
-
bioRxiv - Genomics 2024Quote: ... PacBio WGS HiFi reads were downloaded from HGSVC2 (http://ftp.1000genomes.ebi.ac.uk/vol1/ftp/data_collections/HGSVC2/working/20191005_YRI_PacBio_NA1924 0_HiFi/). Illumina WGS (30X coverage ...
-
bioRxiv - Microbiology 2024Quote: ... 2 µL of 10 µM 3’ PacBio round 2 reverse primer (PacBio_3pri_RND2), and 25 µL KOD Hot Start Master Mix ...
-
bioRxiv - Microbiology 2024Quote: ... 1 µL of 10 µM 5’ nucleotide tagging primer (PacBio_5pri_G or PacBio_5pri_C), 1 µL of 10 µM 3’ nucleotide tagging primer (PacBio_3pri_C or PacBio_3pri_G) ...
-
bioRxiv - Microbiology 2024Quote: The index isolate DA14734 was sequenced with long-read whole genome sequencing using the Pacific Biosciences platform (PacBio, Menlo Park, CA). DNA was prepared from overnight cultures using the Qiagen Genomic-Tip 100 ...
-
bioRxiv - Genomics 2024Quote: A SMRTbell library was then constructed with the SMRTbell® prep kit 3.0 (PacBio Ref. No. 102-141-700), following the manufacturer’s protocol ...
-
bioRxiv - Genomics 2024Quote: The Sequel® II binding kit 3.2 (PacBio Ref. No. 102-194-100) was used for final preparation of sequencing ...
-
bioRxiv - Genomics 2024Quote: ... A SMRTbell library was then prepared by following the protocol of the SMRTbell® prep kit 3.0 (PacBio Ref. No. 102-141-700). Briefly ...
-
bioRxiv - Genomics 2024Quote: ... Ligation of T-overhang SMRTbell adapters was then performed and the SMRTbell library was purified with SMRTbell® cleanup beads (PacBio Ref. No. 102158-300). The concentration and size of the library were examined with the Qubit® Fluorometer and Qubit™ dsDNA HS and BR Assay Kits (Invitrogen™ Cat ...
-
bioRxiv - Genomics 2024Quote: ... The movie was captured by the software SMRT Link v11.0 (PacBio) and HiFi reads are generated and collected for further analysis ...
-
bioRxiv - Genomics 2024Quote: ... The DNA sample was eluted in 80 µL elution buffer (PacBio Ref. No. 101-633-500) and its quantity and quality was assessed with NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer ...
-
bioRxiv - Genomics 2024Quote: A final library preparation was performed with The Sequel® II binding kit 3.2 (PacBio Ref. No. 102-194-100) before sequencing ...
-
bioRxiv - Genomics 2024Quote: ... The sheared DNA sample was purified using SMRTbell® cleanup beads (PacBio Ref. No. 102158-300), from which 2 µL of sample was taken for quality check through overnight pulse-field gel electrophoresis and Qubit® Fluorometer quantification ...
-
bioRxiv - Genomics 2024Quote: ... The sample was then eventually eluted with 120 μl of elution buffer (PacBio Ref. No. 101-633-500). Throughout the extraction progress ...
-
bioRxiv - Immunology 2024Quote: ... the 16S rRNA gene amplicons were generated with the barcoded Full-Length 16S rRNA gene primers (forward: AGRGTTYGATYMTGGCTCAG, reverse: RGYTACCTTGTTACGACTT) from PacBio and the 2x Roche KAPA HiFi Hot Start Ready Mix ...
-
bioRxiv - Cancer Biology 2024Quote: ... using the HiFi protocol (PacBio, Menlo Park, CA, USA). DNA was extracted using the PacBio Nanobind Tissue Kit according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... Raw sequencing data was aligned to the GRCh38/hg38 build of the human reference genome using pbmm2 as part of SMRTLink (PacBio) v 13 and structural variants were detected using pbsv ...
-
bioRxiv - Cancer Biology 2024Quote: The following section of the sequencing preparation was completed using kit components from the MAS-Seq for 10x Single Cell 3’ kit (PacBio, cat. no. 102-659-600), as well as individually created oligos ...
-
bioRxiv - Cancer Biology 2024Quote: ... MAS-Seq for 10x Single Cell 3’ kit (PacBio, cat. no. 102-659-600), and individually created oligos ...
-
bioRxiv - Cancer Biology 2024Quote: The following section of the sequencing preparation was completed using kit components from the MAS-Seq for 10x Single Cell 3’ kit (PacBio, cat. no. 102-659-600), as well as individually created oligos ...
-
bioRxiv - Cancer Biology 2024Quote: ... reads downloaded from the European Genome-Phenome Archive (EGA) (Freeberg et al. 2022) under accessions EGAD00001009814 (PacBio) and EGAD00001009815 (Illumina) ...
-
bioRxiv - Plant Biology 2024Quote: ... CLR (continuous long reads) assemblies were generated using FALCON Unzip from PacBio SMRT Tools 9.0 Suite 15 and CCS (circular consensus sequencing ...
-
bioRxiv - Microbiology 2024Quote: ... 45 isolates were subjected to long read sequencing on the Pacific Biosciences (PacBio, Menlo Park, California, USA) Sequel IIe platform at the Leiden Genome Technology Center ...
-
bioRxiv - Microbiology 2024Quote: ... Size-selection was performed on library sub-pools using either diluted AMPure PBbeads (PacBio, 35% beads, 3.1x v/v ratio) or Blue Pippin (Sage Science ...
-
bioRxiv - Genomics 2024Quote: ... to reduce potential PCR biases of the amplification polymerases A/B supplied by PacBio, we prepared two further libraries using KOD Xtreme™ Hot Start DNA Polymerase C (Merck) ...
-
bioRxiv - Genomics 2024Quote: ... DNA was then isolated from the washed blood pellet using the Circulomics Nanobind CBB kit and protocol according to the manufacturer’s recommendations (Circulomics, now PacBio company). High molecular weight DNA was eluted from the Nanodisk with 150µl Tris-cl buffer ...
-
bioRxiv - Microbiology 2024Quote: ... The 16S rRNA sequences recovered from PacBio raw reads dataset are available in doi:10.6084/m9.figshare.25577322.
-
bioRxiv - Genomics 2024Quote: ... A SMRTbell library was constructed for both samples using the SMRTbell® prep kit 3.0 (PacBio Cat. No. 102-141-700), following the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2024Quote: ... Qualified libraries were sequenced using the BGI Sequel II system (PacBio platform) in HiFi mode.
-
bioRxiv - Genomics 2024Quote: ... Fragmented DNA in the extracted DNA was removed using the Short Read Eliminator Kit (PacBio, CA, US). The sequencing library for long-read sequencing was prepared using the Ligation Sequencing Kit (SQK-LSK109 ...
-
bioRxiv - Plant Biology 2024Quote: ... SMRT sequencing libraries were prepared with the SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) and sequenced on a PacBio Sequel II system ...
-
bioRxiv - Microbiology 2024Quote: ... Sequencing libraries were prepared following the protocol “Procedure & Checklist – Preparing HiFi SMRTbell Libraries from Ultra-Low DNA Input” (PacBio, Menlo Park, CA) followed by inspection with a FEMTOpulse ...
-
bioRxiv - Genomics 2024Quote: ... reference 10x barcodes provided by PacBio were used (https://downloads.pacbcloud.com/public/dataset/MAS-Seq/REF-10x_barcodes/3M-february-2018-REVERSE-COMPLEMENTED.txt.gz) ...
-
bioRxiv - Genomics 2024Quote: The scISO-seq reads were processed using the methods and workflow provided by PacBio (https://github.com/PacificBiosciences/pbbioconda ...