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Citations for PacBio :
101 - 150 of 1293 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: The final library preparation for sequencing was performed with The Sequel® II binding kit 3.2 (PacBio Ref. No. 102-194-100). In brief ...
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bioRxiv - Plant Biology 2024Quote: ... The final library was prepared with the Sequel® II binding kit 3.2 (PacBio Ref. No. 102-194-100) and was loaded with the diffusion loading mode with the on-plate concentration set at 90 pM on the Pacific Biosciences SEQUEL IIe System ...
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bioRxiv - Plant Biology 2024Quote: ... for 6 passes of centrifugation at 1,990 x g for 2 min and was subsequently purified with SMRTbell® cleanup beads (PacBio Ref. No. 102158-300). 2 μL sheared DNA was taken for fragment size examination through overnight pulse-field gel electrophoresis ...
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bioRxiv - Plant Biology 2024Quote: ... Two SMRTbell libraries were constructed with the SMRTbell® prep kit 3.0 (PacBio Ref. No. 102-141-700), following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... After the DNA was eluted with 60 μL elution buffer (PacBio Ref. No. 101-633-500), quality check was carried out with NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer ...
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bioRxiv - Genomics 2024Quote: ... FIX variants were then assigned to sequenced barcodes using the barcode-variant map from PacBio sequencing ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 322 non-duplicated complete and closed (single contig) genome assemblies were selected for having (i) long-read sequencing (Nanopore or PacBio) or (ii ...
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bioRxiv - Genetics 2024Quote: ... Fragments smaller than 40 kb were depleted from these samples using the SRE XL kit (PacBio, SKU102-208-400) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: High molecular weight (HMW) was extracted using PacBio’s Nanobind CBB Kit (Pacbio, 102-301-900) from 2 x 106 cells with the Nanobind adherent cultured cells protocol ...
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bioRxiv - Neuroscience 2024Quote: ... the library preparation was performed SMRTbell prep kit 3.0 (PacBio, 102-141-700) with a PEG wash ...
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bioRxiv - Neuroscience 2024Quote: ... with the Nanobind CBB Kit (PacBio, SKU 102-301-900) and the UHMW DNA Aux Kit (Circulomics/PacBio ...
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bioRxiv - Neuroscience 2024Quote: ... The extracted UHMW DNA was then taken straight into library preparation for sequencing using Oxford Nanopore Technologies (ONT) SQK-ULK001 Kit and the Nanobind Ultra Long Library Preparation Kit (Circulomics/PacBio, NB-900-601-01, no longer available). The library was split into 3 tubes of 75 µl each ...
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bioRxiv - Neuroscience 2024Quote: Ultra-high molecular weight DNA (UHMW) was extracted from the WIBR3 (parental) hESC line (5 x 106 cells) following the Circulomics/Pacific Biosciences (PacBio) UHMW DNA Nanobind Extraction protocol (Circulomics/PacBio ...
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bioRxiv - Neuroscience 2024Quote: ... and the UHMW DNA Aux Kit (Circulomics/PacBio, NB-900-101-01, no longer available). The extracted DNA was checked using the Qubit dsDNA BR assay (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... and size selected with the PacBio SRE Kit (Pacbio, SKU 102-208-300). Following quality control ...
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bioRxiv - Neuroscience 2024Quote: ... UHMW DNA Nanobind Extraction protocol (Circulomics/PacBio, no longer available) with the Nanobind CBB Kit (PacBio ...
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bioRxiv - Genetics 2024Quote: ... The constructed library was sequenced on PacBio Sequel platform (PacBio, USA). Fragmentation and amplification of the ATAC-seq libraries were conducted according to Buenrostro et al ...
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bioRxiv - Genomics 2024Quote: ... The library was bound to polymerase using the Sequel II Binding Kit 2.0 (PacBio) and loaded onto PacBio Sequel II ...
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bioRxiv - Genomics 2024Quote: ... The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer-recommended protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA samples were prepared for PacBio SMRT-Bell sequencing by following the standard PacBio sequencing protocol (https://www.pacb.com/wp-content/uploads/Procedure-Checklist-Preparing-SMRTbell-Libraries-using-PacBio-Barcoded-Overhang-Adapters-for-Multiplexing-Amplicons.pdf) ...
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bioRxiv - Genomics 2024Quote: ... Euclidean distance was measured between any two same cells from PacBio and Illumina on a UMAP ...
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bioRxiv - Genomics 2024Quote: ... Both gene count matrices from PacBio were then subsequently used for comparison with Illumina data subsetted to only protein coding genes and common cells ...
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bioRxiv - Genomics 2024Quote: ... The sequencing was setup and performed in 30-hour movies (with 120 min pre-extension) with the software SMRT Link v11.0 (PacBio). HiFi reads are generated and collected for further analysis ...
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bioRxiv - Plant Biology 2024Quote: ... using the kinetics data from PacBio CCS subreads obtained during HMW DNA sequencing ...
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bioRxiv - Plant Biology 2024Quote: ... The chloroplast genome was assembled from PacBio reads using Organelle_PBA [67,68] ...
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bioRxiv - Plant Biology 2024Quote: ... we proceeded with the de novo assembly of long reads from PacBio HiFi Sequencing data using HiCanu63,64 optimized for high-fidelity long reads ...
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bioRxiv - Genomics 2024Quote: ... Four generations of full-sib matings were carried out to produce the genomic strain SaPa_ori_Rep25-2-1-1 (“Sapa_PacBio”). Fly strains were maintained at room temperature on standard cornmeal food provided by the UC Davis Fly Kitchen with filter paper for environment structure and pupariation substrate.
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bioRxiv - Evolutionary Biology 2024Quote: ... The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The library was bound to polymerase using the Sequel II Binding Kit 2.0 (PacBio) and loaded onto PacBio Sequel II) ...
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bioRxiv - Genomics 2024Quote: ... it can be applied to any collection of genomic sequences (e.g., individual long-reads) from different technologies (e.g., PacBio, Nanopore).
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bioRxiv - Genomics 2024Quote: ... The sample was eluted with 120 μl of elution buffer (PacBio Ref. No. 101-633-500). When DNA was transferred at any time throughout the extraction progress ...
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bioRxiv - Systems Biology 2024Quote: ... OD for each pool was used to combine the hORF pools with volume determined by the library complexity (ie as the number of unique plasmids found in the PacBio barcode-ORF sequencing) and this pool were cultured at 37C overnight (15 hours ...
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bioRxiv - Systems Biology 2024Quote: ... For long-read sequencing (PacBio and Nanopore) used to annotate DNA barcodes with the linked ORF they represent ...
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bioRxiv - Microbiology 2024Quote: ... Barcoded sequencing libraries were prepared using the SMRTbell prep kit 3.0 (PacBio catalog no.102-141-700) with SMRTbell barcoded adapter 3.0 (PacBio catalog no.102-009-200 ...
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bioRxiv - Microbiology 2024Quote: ... with SMRTbell barcoded adapter 3.0 (PacBio catalog no.102-009-200) and using the Covaris g-TUBE shearing method to generate DNA fragments of 7-12 kb in length ...
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bioRxiv - Microbiology 2024Quote: Extraction of high molecular weight DNA from overnight cultures of CPs was accomplished using the Nanobind CBB kit (PacBio catalog no.102-301-900). Barcoded sequencing libraries were prepared using the SMRTbell prep kit 3.0 (PacBio catalog no.102-141-700 ...
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bioRxiv - Microbiology 2024Quote: ... Samples with many short DNA fragments were further purified using either the Short Read Eliminator kit (PacBio, San Diego, CA, USA), or 0.4 - 0.5 x volume KAPA PURE SPRI beads (Roche ...
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bioRxiv - Microbiology 2024Quote: ... Fragments below 10kb were removed with the Short Read Eliminator kit (PacBio). Samples were diluted and equimolarly barcoded using the SQK-RBK114.96 (Oxford Nanopore Technologies ...
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bioRxiv - Plant Biology 2024Quote: ... origin contigs were filtered out from the primary contig assembly using ‘Kerman’ plastid and mitochondrial sequences that were pulled out from PacBio HiFi reads in Geneious Prime (https://www.geneious.com) ...
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bioRxiv - Plant Biology 2024Quote: ... A Pacific Biosciences (PacBio) SMRTbell Express library was prepared according to the manufacturer’s instructions using AMPure PB beads for size selection ...
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bioRxiv - Plant Biology 2024Quote: ... After subread generation (PacBio single-molecule real-time [SMRT] Link v.10.1 ...
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bioRxiv - Genomics 2024Quote: ... one PacBio Iso-seq library was prepared using the SMRTbell® prep kit (PacBio, Menlo Park, CA, United States) and sequenced on the Sequel IIe.
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bioRxiv - Genomics 2024Quote: ... This can be explained by the different methods of sequencing (CLR PacBio reads) and assembly software used for the QQ74 genome assembly which resulted in lower sequence completeness and contiguity in comparison to the new genomes produced with PacBio HiFi sequencing and assembly technology supported by the newest Bionano optical maps for scaffolding (Table 2).
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bioRxiv - Genomics 2024Quote: ... we integrated data from Illumina RNA-Seq and full-length isoform sequencing (PacBio Iso-Seq). We also quantified and compared global protein expression using the DIA method ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Sequencing was done by PacBio HiFi technology to obtain from 69 to 99 GB data.We assembled haplotype resolved contigs using Hifiasm47 with Pacbio HiFi reads and Hi-C reads ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the recommended SMRTbell Express Template Prep Kit 2.0 (PacBio) was used to prepare the libraries ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Libraries were sequenced in a Sequel II (PacBio), using a 8M SMRT cell ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The purified DNA was size selected for >40kb fragments with the PacBio SRE XL buffer (Catalog #102-208-400, PacBio, Menlo Park, California). Long-read sequencing libraries were then generated by Oxford Nanopore R10.4.1 sequencing ...
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bioRxiv - Genomics 2024Quote: ... was used to compute the k-mers from PacBio HiFi reads with k = 21 settings ...
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bioRxiv - Genomics 2024Quote: ... primary assemblies were constructed from PacBio long reads using Flye[94] ...