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Citations for PacBio :
1 - 50 of 1293 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: We used long reads generated by PacBio HiFi sequencing and Nanopore MinION sequencing to close the gaps in pseudomolecules ...
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bioRxiv - Neuroscience 2024Quote: ... the library preparation was performed SMRTbell prep kit 3.0 (PacBio, 102-141-700) with a PEG wash ...
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bioRxiv - Neuroscience 2024Quote: ... with the Nanobind CBB Kit (PacBio, SKU 102-301-900) and the UHMW DNA Aux Kit (Circulomics/PacBio ...
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bioRxiv - Neuroscience 2024Quote: ... The extracted UHMW DNA was then taken straight into library preparation for sequencing using Oxford Nanopore Technologies (ONT) SQK-ULK001 Kit and the Nanobind Ultra Long Library Preparation Kit (Circulomics/PacBio, NB-900-601-01, no longer available). The library was split into 3 tubes of 75 µl each ...
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bioRxiv - Neuroscience 2024Quote: Ultra-high molecular weight DNA (UHMW) was extracted from the WIBR3 (parental) hESC line (5 x 106 cells) following the Circulomics/Pacific Biosciences (PacBio) UHMW DNA Nanobind Extraction protocol (Circulomics/PacBio ...
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bioRxiv - Neuroscience 2024Quote: ... and the UHMW DNA Aux Kit (Circulomics/PacBio, NB-900-101-01, no longer available). The extracted DNA was checked using the Qubit dsDNA BR assay (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... and size selected with the PacBio SRE Kit (Pacbio, SKU 102-208-300). Following quality control ...
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bioRxiv - Neuroscience 2024Quote: ... UHMW DNA Nanobind Extraction protocol (Circulomics/PacBio, no longer available) with the Nanobind CBB Kit (PacBio ...
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bioRxiv - Neuroscience 2024Quote: High molecular weight (HMW) was extracted using PacBio’s Nanobind CBB Kit (Pacbio, 102-301-900) from 2 x 106 cells with the Nanobind adherent cultured cells protocol ...
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bioRxiv - Genomics 2024Quote: ... A SMRTbell library was constructed using the SMRTbell® prep kit 3.0 (PacBio Ref. No. 102-141-700), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... a SMRTbell library was constructed using the SMRTbell® prep kit 3.0 (PacBio Ref. No. 102-141-700), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... followed by DNA purification with SMRTbell® cleanup beads (PacBio Ref. No. 102158-300). 2 µL sheared DNA was used to perform overnight pulse-field gel electrophoresis while the remaining sheared DNA was stored in a 2 mL DNA LoBind® Tube (Eppendorf Cat ...
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bioRxiv - Genomics 2024Quote: ... The Sequel® II binding kit 3.2 (PacBio Ref. No. 102-194-100) was used for final preparation for sequencing ...
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bioRxiv - Genomics 2024Quote: ... The resulting DNA was eluted with 80 µL elution buffer (PacBio Ref. No. 101-633-500) and was subject to quality check using the NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer ...
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bioRxiv - Genomics 2024Quote: ... the SMRTbell library was purified with SMRTbell® cleanup beads (PacBio Ref. No. 102158-300). The concentration and size of the library were examined using the Qubit® Fluorometer and Qubit™ dsDNA HS and BR Assay Kits (Invitrogen™ Cat ...
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bioRxiv - Genomics 2024Quote: The final library preparation for sequencing was performed with The Sequel® II binding kit 3.2 (PacBio Ref. No. 102-194-100). In brief ...
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bioRxiv - Systems Biology 2024Quote: ... OD for each pool was used to combine the hORF pools with volume determined by the library complexity (ie as the number of unique plasmids found in the PacBio barcode-ORF sequencing) and this pool were cultured at 37C overnight (15 hours ...
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bioRxiv - Systems Biology 2024Quote: ... For long-read sequencing (PacBio and Nanopore) used to annotate DNA barcodes with the linked ORF they represent ...
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bioRxiv - Neuroscience 2024Quote: ... This way the gtf files from PACBIO long-reads and the NFIN db reference transcripts were efficiently merged (Rajagopal et al. ...
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bioRxiv - Plant Biology 2024Quote: ... we proceeded with the de novo assembly of long reads from PacBio HiFi Sequencing data using HiCanu63,64 optimized for high-fidelity long reads ...
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bioRxiv - Genomics 2024Quote: ... Both gene count matrices from PacBio were then subsequently used for comparison with Illumina data subsetted to only protein coding genes and common cells ...
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bioRxiv - Genomics 2024Quote: ... The sequencing was setup and performed in 30-hour movies (with 120 min pre-extension) with the software SMRT Link v11.0 (PacBio). HiFi reads are generated and collected for further analysis ...
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bioRxiv - Genomics 2024Quote: ... Euclidean distance was measured between any two same cells from PacBio and Illumina on a UMAP ...
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bioRxiv - Microbiology 2024Quote: ... Samples with many short DNA fragments were further purified using either the Short Read Eliminator kit (PacBio, San Diego, CA, USA), or 0.4 - 0.5 x volume KAPA PURE SPRI beads (Roche ...
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bioRxiv - Microbiology 2024Quote: ... Barcoded sequencing libraries were prepared using the SMRTbell prep kit 3.0 (PacBio catalog no.102-141-700) with SMRTbell barcoded adapter 3.0 (PacBio catalog no.102-009-200 ...
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bioRxiv - Microbiology 2024Quote: ... with SMRTbell barcoded adapter 3.0 (PacBio catalog no.102-009-200) and using the Covaris g-TUBE shearing method to generate DNA fragments of 7-12 kb in length ...
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bioRxiv - Microbiology 2024Quote: Extraction of high molecular weight DNA from overnight cultures of CPs was accomplished using the Nanobind CBB kit (PacBio catalog no.102-301-900). Barcoded sequencing libraries were prepared using the SMRTbell prep kit 3.0 (PacBio catalog no.102-141-700 ...
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bioRxiv - Genetics 2024Quote: ... The constructed library was sequenced on PacBio Sequel platform (PacBio, USA). Fragmentation and amplification of the ATAC-seq libraries were conducted according to Buenrostro et al ...
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bioRxiv - Genomics 2024Quote: ... The library was bound to polymerase using the Sequel II Binding Kit 2.0 (PacBio) and loaded onto PacBio Sequel II ...
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bioRxiv - Genomics 2024Quote: ... The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer-recommended protocol ...
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bioRxiv - Genomics 2024Quote: ... Four generations of full-sib matings were carried out to produce the genomic strain SaPa_ori_Rep25-2-1-1 (“Sapa_PacBio”). Fly strains were maintained at room temperature on standard cornmeal food provided by the UC Davis Fly Kitchen with filter paper for environment structure and pupariation substrate.
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bioRxiv - Evolutionary Biology 2024Quote: ... 322 non-duplicated complete and closed (single contig) genome assemblies were selected for having (i) long-read sequencing (Nanopore or PacBio) or (ii ...
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bioRxiv - Genetics 2024Quote: ... Fragments smaller than 40 kb were depleted from these samples using the SRE XL kit (PacBio, SKU102-208-400) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... The final library was prepared with the Sequel® II binding kit 3.2 (PacBio Ref. No. 102-194-100) and was loaded with the diffusion loading mode with the on-plate concentration set at 90 pM on the Pacific Biosciences SEQUEL IIe System ...
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bioRxiv - Plant Biology 2024Quote: ... for 6 passes of centrifugation at 1,990 x g for 2 min and was subsequently purified with SMRTbell® cleanup beads (PacBio Ref. No. 102158-300). 2 μL sheared DNA was taken for fragment size examination through overnight pulse-field gel electrophoresis ...
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bioRxiv - Plant Biology 2024Quote: ... Two SMRTbell libraries were constructed with the SMRTbell® prep kit 3.0 (PacBio Ref. No. 102-141-700), following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... After the DNA was eluted with 60 μL elution buffer (PacBio Ref. No. 101-633-500), quality check was carried out with NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer ...
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bioRxiv - Plant Biology 2024Quote: ... using the kinetics data from PacBio CCS subreads obtained during HMW DNA sequencing ...
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bioRxiv - Plant Biology 2024Quote: ... The chloroplast genome was assembled from PacBio reads using Organelle_PBA [67,68] ...
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bioRxiv - Genetics 2024Quote: ... KOREF (Illumina HiSeq X Ten / PacBio_SMRT Sequel II) and executed ntRoot (v1.0.0 ...
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bioRxiv - Microbiology 2024Quote: ... as implemented in the SMRT Portal (PacBio). The resulting genome was polished three times using Quiver (PacBio ...
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bioRxiv - Microbiology 2024Quote: ... The resulting genome was polished three times using Quiver (PacBio) before being error corrected with Illumina reads using Snippy v3.2 (https://github.com/tseemann/snippy) ...
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bioRxiv - Microbiology 2024Quote: ... extracted DNA was prepared using the Template Prep Kit 1.0 (PacBio) and following adapter ligation DNA was size selected using a BluePippin system (Sage Biosciences ...
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bioRxiv - Genomics 2024Quote: Fastq files from PacBio reads were downloaded from the ENCODE Project data portal (https://www.encodeproject.org/)38 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Samples were prepared for PacBio sequencing (PacBio Sequel SMRTcell, 20h, v3 chemistry) and were sequenced at the MIT BioMicroCenter ...
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bioRxiv - Molecular Biology 2024Quote: ... a long-read sequence library was prepared using the SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) and sequenced using a PacBio Sequel II system (PacBio ...
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bioRxiv - Molecular Biology 2024Quote: ... and sequenced using a PacBio Sequel II system (PacBio, Menlo Park, CA, USA). The obtained HiFi reads were assembled using Hifiasms with default parameters ...
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bioRxiv - Molecular Biology 2024Quote: ... 2014) was used to create a new structural annotation integrating data from de novo transcriptomes (based on Illumina and PacBio reads obtained in this study), ab initio gene prediction and database gene models ...
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bioRxiv - Genomics 2024Quote: ... it can be applied to any collection of genomic sequences (e.g., individual long-reads) from different technologies (e.g., PacBio, Nanopore).
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bioRxiv - Genomics 2024Quote: ... FIX variants were then assigned to sequenced barcodes using the barcode-variant map from PacBio sequencing ...