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Citations for Promega :
4901 - 4950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... proteins were in-gel digested with trypsin (Promega, V5113). Tryptic peptides were purified by STAGE tips prior to LC-MS/MS measurements ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were harvested and assayed by the Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s instructions 48 h after transfection.
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bioRxiv - Biochemistry 2023Quote: ... Proteins were digested by trypsin (Promega) over night at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... followed by Luciferase Assay Reagent (Promega, E1501). The luciferase activity was measuring using CLARIOstar (BMG Labtech ...
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bioRxiv - Cell Biology 2023Quote: ... with HaloTag amplified by PCR from pHTN-HaloTag CMV-neo (Promega) and mEmerald amplified by PCR from mEmerald-Sec61b-C1 (Addgene #90992) ...
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bioRxiv - Biochemistry 2023Quote: ... Luciferase activity was measured with DualGlo Luciferase Reporter Assay (Promega, #E2920). For knockdown assays ...
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bioRxiv - Cancer Biology 2023Quote: ... and 0.3 ug renilla luciferase control plasmid pRL-CMV (Promega) on an Amaxa Nucleofector 2b (Lonza) ...
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bioRxiv - Cancer Biology 2023Quote: ... and program DLR-2-INJ on a Glomax 20/20 Luminometer (Promega) with 20μl cell extract as the input.
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bioRxiv - Immunology 2023Quote: ... Cytotoxicity was determined by lactate dehydrogenase assay (CytoTox 96, Promega, Cat. G1780). All samples and reagents were brought to room temperature before analyzing.
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bioRxiv - Cell Biology 2023Quote: ... the plate was assayed using the Dual-Glo Luciferase Assay System according to the manufacturer’s instructions (Promega, E2920) and read using a Microplate Luminometer (BioTek ...
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bioRxiv - Cell Biology 2023Quote: ... the NF-κB luciferase reporter was purchased from Promega (#E8491), the Renilla luciferase plasmid (pRL-SV40 ...
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bioRxiv - Cell Biology 2023Quote: ... Phosphorylation of eIF2α was induced by DTT (Promega, DMSO) at 5 mM for 2 h or by UV irradiation at 20 mJ/cm2 using Stratalinker 1800 (Stratagene ...
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bioRxiv - Cell Biology 2023Quote: ... Nucleofected cells were incubated for 48 h prior to clonal amplification and screening for homozygous clones using target-specific PCR (GoTaq Hot Start, Promega) on genomic DNA using the following primers ...
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bioRxiv - Cell Biology 2023Quote: ... 2-3 µg bacmid DNA was transfected (FuGene HD, Promega) into 2 ml Sf9 cells plated at 0.5x106 cells/ml density in a 6-well plate and left to incubate for 5-7 days 27ºC without shaking ...
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bioRxiv - Biochemistry 2023Quote: ... Probes were radiolabelled by means of the Prime-a-Gene labelling system (Promega).
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bioRxiv - Cancer Biology 2023Quote: ... The plasmids were purified using Pure Yield™ Plasmid Midiprep system (A2495, Promega) and their concentration were determined using NanoDrop™ One (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2023Quote: ... drug-containing media was removed and replaced with 10% CellTiter-Blue cell viability assay media (Promega) and after 3-hour incubation at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were mixed with 50 microliters of Stead-Glo® (Promega, E2510) Luciferase Assay System buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Cells viability was measured using the Cell Titer-Glo® Luminescent Cell Viability Assay kit (Promega, G7750). Nano-luminescence values were normalized to corresponding viability data.
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bioRxiv - Evolutionary Biology 2023Quote: ... The amplicons generated from the 5’ and 3’ race PCRs were subjected to purification and subsequent cloning into the pGEM-T easy vector (Promega Corp., WI, USA). The constructed vectors were sequenced (Plasmidsaurus ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected using Viafect (Promega) and imaged 72 hours a;er transfection with mCherry-α-tubulin (gi ...
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bioRxiv - Cell Biology 2023Quote: ... Caspase-Glo® 3/7 reagent (Promega) was added to each well as per manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... Firefly and Renilla luciferase activities were measured using the Dual-Glo Luciferase Assay System (Promega). Renilla activity was used to normalize luciferase reporter activity ...
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bioRxiv - Cancer Biology 2023Quote: ... After 72 hours cell viability was determined using a CellTiter-Fluor™ Cell Viability Assay (Promega) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... 4 µl RT buffer 5x (Promega), 2.5 μl dNTPs 2.5 mM ...
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bioRxiv - Cell Biology 2023Quote: ... 0.2 μl Random hexamers 500 μg/ml (Promega) and 0.5 μl Riboblock RNase inhibitor 40 u/μl (Fermentas ...
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bioRxiv - Cell Biology 2023Quote: ... in a GloMax 20/20 luminometer (Promega). RLuc/FLuc activity values represent the mean ratio of RLuc luminescence normalized against FLuc expressed as percentage of the ratio of the cells transfected with the λN-V5 empty vector.
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed 24 h after transfection and luciferase activities were measured with the Dual-Luciferase Reporter Assay System (Promega) in a GloMax 20/20 luminometer (Promega) ...
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bioRxiv - Cancer Biology 2023Quote: ... cell viability was assessed using CellTiter-Glo Luminescent Cell Viability Assay (#G7572, Promega) and luminescence values were obtained using Infinite M200Pro plate reader (Tecan) ...
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bioRxiv - Cancer Biology 2023Quote: ... each well was treated with 1:1 CellTiter-Glo (Promega, UK) and incubated for 10mins at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Caspase 3/7 activities were detected by Apo-ONE Homogeneous Caspase-3/7 Assay kit (Promega) according to the manufacturer’s protocols.
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bioRxiv - Cancer Biology 2023Quote: ... and assay buffer (Promega). Tumour samples were lysed by rapid shaking using a high-speed benchtop reciprocating homogenizer (Fastprep-24 Sample Preparation Instrument ...
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bioRxiv - Bioengineering 2023Quote: ... Glutamate and glutamine concentration was determined using the Glutamine/Glutamate-Glo Assay (Promega). Metabolite concentration was normalized by the cell number at each time point.
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bioRxiv - Bioengineering 2023Quote: ... and Trypsin Gold (Promega, V5280). The protease was added at a 1:20 enzyme to substrate ratio and LC-MS grade water was added to maintain its concentration at 20 ng/uL ...
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bioRxiv - Cancer Biology 2023Quote: ... CellTiter-Blue® assay (Promega) reagent was added and incubated for 1.5h ...
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bioRxiv - Cancer Biology 2023Quote: ... Luciferase assays were performed by co-transfecting HEK-293T cells with 100 ng pCMV6-empty or pCMV6-miR-497/195 with 10 ng of psiCHECK2 reporter plasmids using FuGENE6 (E2691; Promega) and performing the Dual-Luciferase Reporter Assay System (E1910 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was determined using CellTiter-Glo® Luminescent Cell Viability Assay (Promega, Madison, USA) according to the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2023Quote: ... CellTiter-Glo® 3D Cell Viability Assay (Promega) was performed according to the manufacturer’s instructions and luminescent signals were acquired with an EnVision plate reader (Perkin Elmer).
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bioRxiv - Biochemistry 2023Quote: ... Proteins were digested for 18 to 24 hours on bead with sequencing grade trypsin (Promega) in 50 mM HEPES buffer at 1:50 trypsin to protein ratio ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were again diluted to 2 M urea and digested with 1 µg trypsin (Promega) (1/100 ...
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bioRxiv - Biophysics 2023Quote: ... The 12-230 kDa separation module was used for PCNA (CST, D3H8P, Rabbit, 1:100 dilution, #13110), b-Actin (CST, D6A8, Rabbit, 1:50 dilution, #8457S) and Halo (Promega, Mouse, 1:10 dilution, #G9211) detection ...
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bioRxiv - Biochemistry 2023Quote: ... and 0.5 μL of RNase inhibitor (Promega). The wells were then washed with PBS with 0.1% (v/v ...
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bioRxiv - Biochemistry 2023Quote: The initial HaloTag-based APMS (HaloMS) was performed using a commercially available HaloTag ligand plate (Promega, HaloLink 96 Well Plate ...
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bioRxiv - Biochemistry 2023Quote: ... according to the manufacturer’s recommendations (Promega). The corresponding human ORF clones were transferred by Gateway LR recombination into pIX-Halo:ccdB and pIX-3×HA:ccdB destination vectors (16) ...
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Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial CellsbioRxiv - Microbiology 2023Quote: ... ARINA-1 cytotoxicity was measured using the CellTiter-Glo® 2.0 Cell Viability Assay (Promega, Cat. # G9241), which measures intracellular ATP ...
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Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial CellsbioRxiv - Microbiology 2023Quote: The cytotoxicity of all compounds (except ARINA-1 that interferes with the assay) was measured using the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, Cat. # G1780), which measures LDH enzyme release ...
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Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial CellsbioRxiv - Microbiology 2023Quote: ... The plates were incubated at 37°C under 5% CO2 for 144 hour and virus cytopathic effect was measured using the CellTiter-Glo® 2.0 Cell Viability Assay (Promega, Cat. # G9241) following the manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... Sequences of all plasmids were verified using a SupreDye v3.1 Cycle Sequencing Kit (M&S TechnoSystems, Cat# 063001) with a Spectrum Compact CE System (Promega). The psPAX2-IN/HiBiT plasmid was a kind gift from Dr ...
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bioRxiv - Microbiology 2023Quote: ... reduced and alkylated with IAM 55 mM before digestion with 0.5 μg of Trypsin (Promega) using the FASP protocol 70 with filter passivation in 5% Tween-20 71 was used for sample preparation ...
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bioRxiv - Microbiology 2023Quote: ... RT), alkylated (55 mM IAM, 45 min, RT, in the dark) and digested with 0.5 μg of trypsin (Promega). Tryptic peptides were desalted using a POROSTM R2 resin (ThermoFisher) ...