Labshake search
Citations for Promega :
4951 - 5000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... at which point cells were analysed with the Bright-Glo™ Luciferase Assay System (Promega; E2610) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... after intraperitoneal injection of VivoGlo™ luciferin (Promega; P1041). Human T cells were transduced at an MOI of 1.5.
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bioRxiv - Synthetic Biology 2023Quote: ... Crude extract (10 μl) was mixed with 40 μl of LARII and FLuc activity was determined using a GloMax®-Multi Detection System (Promega) with a 2 s delay and a 10 s integration times ...
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bioRxiv - Microbiology 2023Quote: ... All three genes were amplified using the Gotaq G2 flexi polymerase (Promega) following the manufacturer’s instructions and sequenced using the reverse PCR primer at GATC Biotech ...
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bioRxiv - Plant Biology 2023Quote: ... and cloned into pTNT (Promega) using EcoRI and SalI restriction sites ...
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bioRxiv - Plant Biology 2023Quote: ... 100 μL of trypsin digestion solution (Promega, 5 μl of reconstituted trypsin were dissolved in 100 μL 50 mM Tris-HCl buffer pH 8 ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were produced in vitro via the TNT SP6 Quick Coupled Transcription/Translation System (Promega) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: The LDH levels in supernatant samples were measured using the CytoTox 96 Non-23 Radioactive Cytotoxicity Assay (Promega, G1780) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... cell viability was measured using CellTiter-Glo (Promega #G7570) according to the manufacturer’s protocol ...
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Engineering synthetic CopT/A-based genetic circuits for miRNA imaging and functional gene regulationbioRxiv - Synthetic Biology 2023Quote: ... 50 μL of coelenterazine substrate was added to the wells and Gluc activity was measured using a Glomax-20/20 luminometer (Promega).
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested off the beads by adding 6 μL of 1 μg/μL sequencing grade Trypsin (Promega), diluted in 50 mM acetic acid ...
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bioRxiv - Systems Biology 2023Quote: ... Cell viability was tested using CellTiter-Glo® reagent (Promega, #G7572) 3 d after selection and the virus titer was determined based on the cell survival rate.
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bioRxiv - Systems Biology 2023Quote: ... 1 unit of Taq polymerase (Promega, Madison, WI, USA), and approximately 75 ng of schistosome genomic DNA ...
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Phosphoproteomics of cellular mechanosensing reveals NFATC4 as a regulator of myofibroblast activitybioRxiv - Systems Biology 2023Quote: ... cDNA was synthesized from total RNA (100 ng) using GoScript™ Reverse Transcriptase System (Promega, #A5000), and the reverse transcription was 42°C for 1h ...
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bioRxiv - Systems Biology 2023Quote: ... samples were in-gel digested with trypsin (sequencing grade, Promega, Mannheim), the resulting peptides extracted by two changes of 5% formic acid (FA ...
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bioRxiv - Systems Biology 2023Quote: ... TX)33 24 hours before analysis using CellTiter-Glo viability assay (Promega, Madison, WI). Response was normalized to a DMSO control.
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bioRxiv - Plant Biology 2023Quote: ... Activities of firefly luciferase (LUC) and Renilla luciferase (REN) were examined using a Dual-Luciferase Reporter Assay System kit (Promega, E1960). LUC/REN was calculated as the relative activity and the ratio of dark / HL was used to indicate light response ...
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Engineering synthetic CopT/A-based genetic circuits for miRNA imaging and functional gene regulationbioRxiv - Synthetic Biology 2023Quote: ... The luciferase activity was measured using a Glomax-20/20 luminometer (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: A 265 bp PHABULOSA fragment in pGEM-T-Easy was PCR amplified with M13 primers to and used for sense and antisene in vitro transcriptions with T7 and SP6 polymerases (Promega) in the presence of 1.2 μM of α-32P-UTP (10μCi/μL ...
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bioRxiv - Plant Biology 2023Quote: Two vials of trypsin/Lys-C mix (100µg, V5078, Promega, Alexandria, NSW, Australia) were dissolved into 1 mL of the resuspension buffer (50mM acetic acid ...
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bioRxiv - Synthetic Biology 2023Quote: ... using the Dual-Glo® Luciferase Assay System (Promega) according to the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2023Quote: ... using the FuGENE® HD transfection reagent (Promega) in Opti-MEM™ I reduced serum medium (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2023Quote: ... Following HaloTag technology protocol (Promega), cells were incubated for 30 min in DMEM 10%FCS supplemented with 0.1 nM JF 549 for SPT ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified using MagneHis nickel particles (Promega, WI), then dialyzed against EMSA buffer (50mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: Cell lysate was prepared by using passive lysis buffer (Promega). Firefly and renilla luciferase activities (Fluc and Rluc ...
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bioRxiv - Microbiology 2023Quote: ... Firefly and renilla luciferase activities (Fluc and Rluc) were measured using a Dual Luciferase Assay kit (Promega) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: pWSK-EV71-GFP was linearized XbaI and EV71-GFP RNAs were transcribed using the T7 RiboMax kit (Promega). After transfection into 293A-SCARB2-L3HYPDH and 293A-SCARB2-Ctrl cells ...
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bioRxiv - Microbiology 2023Quote: ... psiCHECK2-M was a modified form of psiCHECK-2 (Promega) with deletion of the HSV-TK promoter (Figureure 5A) ...
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bioRxiv - Microbiology 2023Quote: ... RNAs were treated with DNase using an RQ1 RNase-Free DNase Kit (Promega); cDNAs were synthesized using PrimeScript RT reagent Kit (Takara ...
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bioRxiv - Bioengineering 2023Quote: Dual reporter activities were measured using the Dual Luciferase Reporter Assay System (Promega, Madison, WI, USA) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... Proteolysis was initiated by adding a 3.8 mM Thermolysin (Promega, #V4001) stock solution to the kinase (final concentration of thermolysin = 60 nM) ...
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bioRxiv - Cancer Biology 2023Quote: ... re-swelled with 0.5 μg of modified trypsin (Promega) in 100 mM NH4HCO3 ...
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bioRxiv - Cancer Biology 2023Quote: ... Relative ATP concentrations were determined using the CellTiter-Glo Luminescent Cell Viability Assay (Promega).
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bioRxiv - Cancer Biology 2023Quote: ... Changes in cAMP concentration were determined using the cAMP-Glo Assay (Promega).
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bioRxiv - Molecular Biology 2023Quote: ... and then diluted to 1M urea with 200 mM ABC for trypsin digestion (Sequence-grade, #V5111, Promega, WI, US) (1 µg ...
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bioRxiv - Biochemistry 2023Quote: ... and 0.4 μL of 0.25 μg/μL trypsin (Promega, proteomics grade) in 25 mM HEPES was added ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were further processed by digesting them overnight with a Trypsin/Lys-C mix (Promega, Madison, USA) at a 50:1 substrate:enzyme ratio (w/w ...
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bioRxiv - Cell Biology 2023Quote: ... The nanoluciferase substrate Nano-Glo Live Cell (Promega) was added to the wells at a final dilution of 1:100 and luminescence signal was read immediately and for several cycles in order to assure the detection of the plateau of the enzyme ...
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bioRxiv - Cell Biology 2023Quote: Plasmids encoding for NanoBiT system (SmBit and LgBit) were purchased from Promega (Madison, USA) and cloned upstream of the tau protein in pcDNA3.1 vectors by restriction enzymes ...
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bioRxiv - Molecular Biology 2023Quote: ... The segment sequences of circPLXNA2 and MDM4 3’UTR containing the putative gga-miR-12207-5P binding sequence were amplified by PCR reaction and then subcloned into the XhoI and SalI restriction sites in the pmirGLO dual-luciferase reporter vector (Promega, USA).
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bioRxiv - Molecular Biology 2023Quote: ... and the amplified sequence fragment was cloned into the commercial vector pcDNA3.1 (Promega, Madison, WI, USA) through the restriction site of HindIII and XhoII.
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bioRxiv - Microbiology 2023Quote: ... and relative luciferase activity measured using Dual luciferase Stop and Glo reagents (Promega) as directed by the manufacturer.
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bioRxiv - Microbiology 2023Quote: ... GoTaq qPCR MasterMix (Promega) and the desired primer set ...
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bioRxiv - Microbiology 2023Quote: ... Protein bands were detected using a G:BOX (Syngene) after treating membranes with ECL Western Blotting Substrates (Promega). Densitometry was performed using ImageJ software.
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bioRxiv - Microbiology 2023Quote: ... samples were lysed with 1x Passive Lysis Buffer (Promega) and relative luciferase activity measured using Dual luciferase Stop and Glo reagents (Promega ...
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bioRxiv - Microbiology 2023Quote: ... agglomerans R1 using the Wizard Genomic DNA Purification Kit (Promega, Germany). All restriction enzymes were obtained from New England Biolabs GmBH and used according to manufacturer’s protocols ...
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bioRxiv - Cell Biology 2023Quote: ... DNase treatment of RNA was performed using RQ1 RNase-free DNase (Promega) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... DNase treatments were performed with RQ1 RNase-Free DNase (Promega #PR-M6101). Three biological replicates were performed for samples of Pst DC3000-or mock-infiltrated leaves ...
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bioRxiv - Neuroscience 2023Quote: ... GloSensor (Promega) 22F cAMP dependent reporter plasmid (Promega) ...
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bioRxiv - Neuroscience 2023Quote: ... then with trypsin (Promega, 1:50 (protease to protein)) for 6 hours on a 37 °C shaker.