Labshake search
Citations for Promega :
5101 - 5150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ABIL emulsion oil was prepared by supplementing mineral oil with 4% ABIL EM 90 (Redox, Australia) and 0.05% Triton X-100 (Promega, United States). 425 μL of ABIL oil was added to the PCR mix ...
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bioRxiv - Immunology 2023Quote: ... 10% (w/v) of PEG8000 (Promega) was added and shaken the tubes until dissolved ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was produced using random hexamers as primers and the GoScriptTM Reverse Transcription kit from Promega. Amplification of 16S rRNA and fcrX cDNA was made using SsoFast EvaGreen Supermix 2X kit (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: RNA was extracted from bacterial culture samples using Maxwell® 16 LEV miRNA Tissue Kit (Promega). cDNA was produced using random hexamers as primers and the GoScriptTM Reverse Transcription kit from Promega ...
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bioRxiv - Microbiology 2023Quote: ... along with pRL-TK Renilla (Renilla Luciferase, internal control; Promega) as an internal transfection control ...
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bioRxiv - Microbiology 2023Quote: ... the cells were co-transfected using Fugene HD transfecting reagent at a 1:3 ratio (Promega) along with pRL-TK Renilla (Renilla Luciferase ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated for 30 min at 4 °C slowing rotating with Passive Lysis Buffer (Promega Cat#E1941). After incubation with the lysis buffers ...
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bioRxiv - Microbiology 2023Quote: ... bovine serum albumin solution (1 μg/μL) (Promega, United States), Lucigen Ready-Lyse lysozyme (500 U/μL ...
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bioRxiv - Microbiology 2023Quote: ... 2 μg of env clone was co-transfected with 4 μg of the pNL4.3-ΔEnv-vpr+-luc+ into 293T cells in a T25 flask using the FuGENE6 transfection reagent (Promega). The cells were cultured at 37°C for 6 hours before the medium was completely replaced with fresh medium ...
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bioRxiv - Immunology 2023Quote: ... pHT315-GFP was then extracted and purified using either the Pureyield plasmid miniprep kit (Promega #A1223) or the QIAGEN® Plasmid Mini Kit (QIAGEN) ...
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bioRxiv - Immunology 2023Quote: ... Luminescence was recorded with a 0.3 second integration time on a GloMax luminometer system (Promega).
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bioRxiv - Immunology 2023Quote: ... Luminescence was recorded with a 0.3 second integration time on a GloMax luminometer system (Promega).
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bioRxiv - Immunology 2023Quote: ... Off-target repair template integration in the genome was also assessed using the Nano-Glo® HiBiT Blotting System (Promega) per manufacturer instructions.
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bioRxiv - Immunology 2023Quote: ... intracellular glutathione levels were measured with the GSH-Glo™ Glutathione Assay kit (Promega), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... and then 50 µL of Glutamate Detection Reagent (Promega, Glutamate-Glo Kit) was added to each well ...
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bioRxiv - Immunology 2023Quote: ... Thereafter the samples were processed according to the Manufacturer’s Protocol for ReliaPrep RNA Tissue Miniprep System (Promega, Fitchburg, USA). PBMCs were isolated using histopaque density gradient centrifugation (Sigma ...
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bioRxiv - Immunology 2023Quote: ... Further processing was according to the Manufacturer Protocol using ReliaPrep RNA Cell Miniprep System (Promega, Fitchburg USA). RNA integrity was analyzed using an Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Immunology 2023Quote: Using Go Script cDNA Kit (Promega, Fitchburg USA) RNA samples were processed according to the manufacturer’s protocol.
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bioRxiv - Immunology 2023Quote: ... Cell viability was assessed by measuring ATP levels using the CellTiter-Glo Luminescent Cell Viability Assay (Promega, G7570) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Lactate dehydrogenase (LDH) release was measured using LDH-Glo Cytotoxicity Assay kit (Promega, J2380) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 2 ng of each purified epicPCR product was amplified using qacE_F (0.4 μM) and AP28_short (0.4 μM) primers in GoTaq polymerase master mix (Promega, United States) under the following thermocycling conditions ...
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bioRxiv - Cell Biology 2023Quote: ... HEK-293 were transfected with NLuc/target fusion constructs using FuGENE HD (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Carrier DNA was encoded in pFN5K vectors (Promega). For cellular BRET target engagement experiments ...
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bioRxiv - Cell Biology 2023Quote: ... tracer K10 (Promega, 0.13 µM). To measure BRET ...
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bioRxiv - Cell Biology 2023Quote: ... PLK2 or PLK3 fusions were encoded in pFN31K expression vectors (Promega). Carrier DNA was encoded in pFN5K vectors (Promega) ...
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bioRxiv - Cell Biology 2023Quote: ... Broad spectrum profiling on 192 kinases was performed using the K192 NanoBRET Target Engagement assay (Promega) with tracer K10 using the published protocol [24].
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bioRxiv - Cell Biology 2023Quote: ... NanoBRET NanoGlo Substrate and Extracellular NLuc Inhibitor (Promega) were added according to the manufacturer’s recommended protocol ...
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bioRxiv - Cell Biology 2023Quote: ... NLuc/target fusion constructs were diluted into Transfection Carrier DNA (Promega) at a mass ratio of 1:9 (mass/mass) ...
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bioRxiv - Cell Biology 2023Quote: ... Cell-Titer Glo (Cat# G7572) was ordered from Promega (Madison, WI). Dimethyl sulfoxide (Cat# 276855 ...
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bioRxiv - Cell Biology 2023Quote: CTG assay was performed according to the package instructions from Promega: 100µL of CTG was added to each well for a 1:1 CTG dilution in RPMI ...
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bioRxiv - Cell Biology 2023Quote: ... using the Dual-Luciferase Reporter Assay System (Promega) and corrected for the luciferase activity of the internal control plasmid ...
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bioRxiv - Cell Biology 2023Quote: ... with pRL-CMV encoding Renilla luciferase (Promega) and luciferase reporter constructs for either gamma-interferon activation site (GAS ...
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bioRxiv - Cell Biology 2023Quote: ... Trypsin Gold (Promega, Madison, WI, USA); Glutathione Sepharose 4B beads (GE Healthcare ...
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bioRxiv - Cancer Biology 2023Quote: ... Caspase-Glo 3/7 assay (Promega) was used according to the manufacturer’s manual ...
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bioRxiv - Cancer Biology 2023Quote: ... Livers were incubated using Luciferase Cell Culture Lysis 5 x Reagent (Promega, E1531) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... (2) DNA template degradation by the DNaseI free (Promega), and (3 ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA was isolated using the ReliaPrep™ FFPE gDNA Miniprep system (Promega) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Bangalore using GenePrint 10 (Promega Corporation), version 3.0.0 of the SoftGenetics ...
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bioRxiv - Cancer Biology 2023Quote: ... using the FUGENE6 transfection reagent (Promega, Madison, WI, USA) in Opti-MEM medium (ThermoFisher) ...
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bioRxiv - Cell Biology 2023Quote: ... TMR-Halo-ligand (G8251; Promega) and Janelia Fluor HaloTag Ligands (GA1120 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells expressing HaloTag constructs were incubated overnight with 10-100 nM Janelia Fluor 646 HaloTag ligand (Promega) (61) ...
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bioRxiv - Cell Biology 2023Quote: ... using a luciferase assay kit (#E1500 Promega, USA) as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... and luciferase activity was measured with a GloMax Navigator microplate luminometer (Promega, USA) using a luciferase assay kit (#E1500 Promega ...
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bioRxiv - Cell Biology 2023Quote: ... the beta-galactosidase enzyme activity was measured using a kit (#E2000, Promega, USA). Luciferase readings were divided with beta-galactosidase readings to normalize for transfection differences ...
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bioRxiv - Cell Biology 2023Quote: ... The HIV-1NL4.3 LTR was cloned into pG5luc (Promega) upstream of the firefly luciferase gene ...
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bioRxiv - Cell Biology 2023Quote: ... The Dual Glo-reporter assay (Promega) was used to measure LTR activation via the Firefly luciferase readout along with signal normalization by Renilla luciferase ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were plated into 96 well plates (3000 cells per well) and cell viability was assessed with a luminescence-based assay (CellTiter Glo, Promega, Cat # G7572) at 48 hours on a SpectraMax M3 plate reader (Molecular Devices) ...
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bioRxiv - Cancer Biology 2023Quote: ... Viability was determined after 5 days using CellTiterGlo reagent (Cat #G9683, Promega) and detected using FluoStar Optima Plate Reader (21999 ...
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bioRxiv - Cell Biology 2023Quote: ... cells from the bottom chamber were collected with Passive Lysis Buffer (Promega), luminescence was measured and DNA extraction was performed as described elsewhere.
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bioRxiv - Cell Biology 2023Quote: ... were analyzed with the addition of luciferin (100mg/mL) or furimazine (Nano-Glo® Luciferase, Promega) diluted 1:200 to 1:500 in 1X PBS ...