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Citations for Promega :
5051 - 5100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: T cell cytotoxicity induced by BiTEs and BiTE-sialidase fusion proteins was measured by lactate dehydrogenase (LDH) release using CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, G1780). 10000 tumor cells and 50000 hPBMCs per well in 100 μL media were exposed to different treatments and incubated in 96 well plates at 37 °C (unless different ratio was specified elsewhere) ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-GFAP (G5601, Promega), guinea pig anti-Ctip 2 (325005 ...
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bioRxiv - Neuroscience 2023Quote: ... PCR analyses of Ambra1 KO gene were performed as described previously(Dere et al., 2014) using GoTaq® G2 Flexi DNA polymerase (Promega). For Ambra1 WT allele ...
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bioRxiv - Neuroscience 2023Quote: ... The following antibodies were used for immuohistochemistry: mouse anti-β-gal (Z3781, Promega), chicken anti-NeuN (266006 ...
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bioRxiv - Neuroscience 2023Quote: ... GoTaq® G2 Flexi DNA polymerase (Promega) with forward primer ...
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bioRxiv - Pathology 2023Quote: ... First-strand cDNA was synthesized with 2.0 µg RNA using M-MLV reverse transcriptase (Promega, Madison, WI, USA). RT-qPCR was performed as reported previously (Cao et al. ...
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bioRxiv - Neuroscience 2023Quote: ... was performed by incubating the proteins first with endoproteinase Lys-C (Wako, #125-05061,)) in 8 M urea for 4h at 37°C followed by an overnight trypsin digestion (Promega #V511A,) in 2 M urea at 37°C ...
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: Programmed cell death was determined in retinal cryosections by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) using the DeadEnd Fluorometric TUNEL system following the manufacturer’s instructions (Promega, Madison, WI, USA). Next ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were lysed for enzymatic cAMP measurement assay using a commercial kit and following manufacturer’s instructions (Promega, #V1501).
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bioRxiv - Pathology 2023Quote: ... firefly luciferase and Renilla luciferase were measured using the Dual-Glo Luciferase Assay (Promega, Cat 377 No. E2920). The luminescence was read on a microplate reader (SpectraMax ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... pTargeT mammalian expression vector and P450-Glo CYP3A4 Assay (Luciferin IPA) were obtained from Promega (Madison, WI). RNAiso and random hexamers were from Takara (Shiga ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cells were washed with PBS and lysed with 50 μL of lysis buffer (Promega). Twenty-five μL of the lysates were transferred to a white plate and mixed with the same volume of Nano-Glo® Luciferase reagent ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cells were transfected with 1 or 1.5 μg plasmid DNA using FuGene HD (Promega, #E2311) according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The cytotoxicity of NBCoV small molecules in the different cell types was performed in parallel with the neutralization assay and evaluated using the colorimetric CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) (Promega, Madison, WI) following the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... and trypsinized (0.5 μg/μl trypsin (Promega) in 50 mM ammonium bicarbonate ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Pseudoviruses capable of single-cycle infection were prepared by transfecting 8×106 HEK293T/17 cells with a proviral backbone plasmid and an envelope expression vector by using FuGENE HD (Promega, Madison, WI) and following the manufacturer’s instructions as previously described46 ...
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bioRxiv - Plant Biology 2023Quote: Dual-luciferase assay was completed using a Dual-Luciferase Reporter Assay System kit (Promega, Madison, USA) by following the manufacturer’s instructions as described previously (Li et al ...
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bioRxiv - Plant Biology 2023Quote: ... The firefly luminescence (LUC) from samples was immediately recorded for 5.0 sec on a GloMax 20/20 luminometer (Promega, Madison, USA) followed by 10-sec interval ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cells were assayed for lactate dehydrogenase activity using CytoTox 96 cytotoxicity assay kit (Promega, G1780). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Luminescence was assessed with the Nano-Glo® HiBiT Lytic Detection System (Promega, N3040) in 384-well plate format and in technical triplicates ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The cytotoxicity of MLNP was evaluated by MTS assay using CellTiter 96® AQueous reagents according to the manufacturer’s protocol (Promega, Madison, WI, USA) and as previously described45 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... coli strains were performed using the PureYield™ Plasmid Miniprep System (Promega Corp., Madison, WI) according to manufacturer protocols ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The concentration at which 50% of cell growth was inhibited (GI50) was determined using Cell TiterGlo (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... on a Maxwell 48 RSC instrument (Promega, Madison, WI) as previously reported [54] ...
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bioRxiv - Pathology 2023Quote: ... and amniotic fluid using the Viral Total Nucleic Acid Purification Kit (Promega, Madison, WI) on a Maxwell 48 RSC instrument (Promega ...
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bioRxiv - Pathology 2023Quote: ... The final reaction mixtures contained 150 ng random primers (Promega, Madison, WI), 600 nM each primer and 100 nM probe ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... HiBiT reagent was prepared according to manufacturer directions (Promega Corp.). Using a multichannel pipette ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... opaque 96-well plates and reagents were added according to the manufacturer’s protocols for the Nano-Glo® HiBiT Extracellular Detection System (Promega Corp.). After a 10-minute incubation step ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cell viability was assessed after 72 h with the CellTiter-Glo assay (Promega, G7573). Survival curves and area under the curve (A.U.C. ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 4 h later 5 µl/well of NanoBRET™ Nano-GloR Substrate (10 µl/ml in DMEM no phenol red, Promega) was added and 460 nm donor and 618 nm acceptor signals were read within 10 min of substrate addition using CLARIOstar microplate reader (Mandel) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Cells used for cAMP experiments were produced by PEI-transfection of HEK 293 cells with the commercially available plasmid pGloSensorTM-22F cAMP (Promega, GU174434). Those cells were additionally PEI-transfected with a plasmid containing the information for the adenosine A1 receptor under control of a CMV promoter ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The following day cells were trypsinized and seeded in 384-well white plate (20 µl/well) in DMEM F12 (no phenol red, 4% FBS) +/-HaloTag® NanoBRET™ 618 Ligand (1 µl/ml, Promega) and +/-compounds (DMSO concentration in each sample was kept the same) ...
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bioRxiv - Molecular Biology 2023Quote: ... and PCR screening was performed using GoTaq Flexi DNA polymerase (Promega, Wisconsin, United States) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 0.5 μg of sequencing grade trypsin (Promega) was added to each sample and incubated at 37°C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... 1μg samples of purified RNA were reverse transcribed into cDNA with M-MLV Reverse Transcriptase (Promega, Madison, WI, USA). 25ng samples of cDNA were then used in SYBR Green real-time PCR reactions on a Step-One real-time PCR machine (Applied Biosystems) ...
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bioRxiv - Neuroscience 2023Quote: ... 1x Protease inhibitor cocktail (50x in 100% Ethanol, G6521, Promega), 0.2 U/µl RNasin Plus (N2615 ...
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bioRxiv - Neuroscience 2023Quote: ... 0.2 U/µl RNasin Plus (N2615, Promega), 0.1% Triton X-100 ...
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bioRxiv - Cell Biology 2023Quote: ... qPCR reactions were performed in a 384-well format using GoTaq qPCR Master Mix (Promega) and run on an ABI PRISM 7900HT real time PCR system (Applied Biosystems) ...
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bioRxiv - Cell Biology 2023Quote: ... Library was quantified by QauntiFluor ssDNA System (Promega). Sequencing of the prepared DNB was conducted on the MGIseq system (MGI ...
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bioRxiv - Cell Biology 2023Quote: ... The double stranded library is quantified using QauntiFluor ONE dsDNA System (Promega). The library is circularized at 37 °C for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... luminescence activity was detectected using Promega OneGlo Luciferase Reagent (Promega). Luminescence values were normalized to DMSO control and data was analyzed using GraphPad Prism.
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bioRxiv - Cell Biology 2023Quote: ... CellTiterGlo assay (Promega) was performed after 6 days according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... Protein was then digested with 2.5 μg Lys-C (Wako, Japan) for 4 h at 37°C and 2 μg trypsin (Promega, V5113) for an additional 4 h at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... and 1cµg/20 µl of RNA was reversely transcribed to cDNA using MMLV reverse transcriptase (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Terminal deoxynucleotidyltransferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) staining of paraffin-embedded tissue sections was done with the DeadEnd Fluorometric TUNEL System (G3250, Promega). TUNEL-positive areas from three fields (magnification×10 ...
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bioRxiv - Cell Biology 2023Quote: ... qPCR reactions were completed using GoTaq qPCR Master Mix (Promega) with the following primer pairs ...
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bioRxiv - Cell Biology 2023Quote: ... The RNase inhibitor RNasin Plus (Promega) was used at 40 U/ml.
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bioRxiv - Cell Biology 2023Quote: ... or 40 U/ml RNase inhibitor (RNasin Plus; Promega) in lysis buffer followed by Western blotting ...
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bioRxiv - Cell Biology 2023Quote: ... double digested Capn4 PCR product was ligated with double digested pCWX200 or pLexA using the LigaFAst Rapid DNA Ligation System (Promega, M8226). The constructs were transformed into E ...
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bioRxiv - Immunology 2023Quote: ... 105 cells per target population were sorted and kept on ice until further processing using GSH-GloTM Glutathione Assay (Promega), strictly following manufacturer instructions ...