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Citations for Promega :
4801 - 4850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... and digested with trypsin and Lysyl endopeptidase (Promega) in a buffer containing 40 mM ammonium bicarbonate ...
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bioRxiv - Genomics 2023Quote: ... DNA was quantified using a Quantus Fluorometer (Promega). All the following steps involving handling DNA were performed with wide-bore pipet tips ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Genomic DNA of the evolved clones was isolated using the Wizard® Genomic DNA Purification Kit (Promega) and short-read sequenced using the Illumina HiSeq platform (Microbial Genomic Sequencing Center ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The proteins were subsequently digested overnight with molecular grade trypsin enzyme (Promega) using a ratio of 1:50µg (w/w ...
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bioRxiv - Genetics 2023Quote: ... 10ng of Renilla vector (Promega) was co-transfected and used for normalization ...
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bioRxiv - Genetics 2023Quote: ... 12.5 µL of Promega Master Mix (Cat# M7502 Promega), 6.5 µL of Molecular Biology Grade water (ref#46-000-CI Corning) ...
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bioRxiv - Genetics 2023Quote: ... SIRT5 WT and variants were purified by Ni2+-affinity resin (#V8823, Promega), and eluted with 25 mM Tris/HCl pH 8.0 ...
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bioRxiv - Genetics 2023Quote: ... and cloned the PCR products into JM109 competent cells using pGEM®-T Easy Vector Systems (Promega Corporation, Madison, WI, USA). Transformed cells were amplified and Sanger sequenced at the Genomics Facility ...
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bioRxiv - Developmental Biology 2023Quote: ... Trypsin Gold (Promega, Madison, WI, USA, Cat# V5820) was added to each sample in a 1:50 ratio (wt/wt ...
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bioRxiv - Microbiology 2023Quote: ... The three PCR fragments were ligated by PCR using primers P1/P3 and cloned into pGEM®-T easy vector (Promega). Plasmid was then digested with NotI ...
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bioRxiv - Microbiology 2023Quote: ... the Nluc and Fluc activities were measured using the Nano-Glo Dual-Luciferase Reporter Assay System (Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: LDH-Glo Cytotoxicity Assay (Promega, WI, USA, Cat. J2380) was performed according to manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... 1 μL of 10 mM dNTPs (Promega, cat. no: U1515), and 0.5 μL of Phusion Polymerase (NEB ...
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bioRxiv - Genetics 2023Quote: ... ∼0.75 kb amplicons were cloned using the pGEM-T Easy vector system (Promega; Madison, WI) following manufacturer’s protocol and the plasmids were then transformed into TOP10 cells (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... 10 U RNAsin (Promega), 1× Superscript II First-Strand Buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... The luciferase reporter assay system (Promega) was used according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... PCR products were cleaned with ExonucleaseI (ExoI)(Fermentas; Pittsburgh, PA) and Shrimp Alkaline Phosphatase (SAP) (Promega; Madison, WI) incubated at 37°C for 30 minutes and 80°C for 15 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... Firefly and Renilla Luciferase activities were quantified using the dual reporter assay kit (Promega) according to the manufacturer’s instructions on an Infinite 200 Pro-luminometer (TECAN) ...
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bioRxiv - Developmental Biology 2023Quote: ... the dual luciferase reporter assay kit (Promega) was used ...
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bioRxiv - Genetics 2023Quote: ... 3,500,000 cells were seeded in 10 cm plates (2-4 per replicate) and transfected with FuGENE® 6 Transfection Reagent (Promega, E2692). In one tube ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were then digested with trypsin (Promega) at 1:50 trypsin/protein ratio for 90 min at 47°C ...
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bioRxiv - Developmental Biology 2023Quote: ... 293T cells were harvested and luciferase and renilla activities were detected using the Dual-luciferase reporter assay kit (Promega) in a Glowmax 20/20 luminometer (Promega) ...
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bioRxiv - Developmental Biology 2023Quote: ... The Carm1-reporter or its mutated reporters was co-transfected into 293T with the pRL-TK plasmid (Promega) expressing renilla for normalization ...
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bioRxiv - Developmental Biology 2023Quote: ... and 0.5 U/ml of RNAsin (Promega). The beads were then resuspended in 10 ml of reverse transcriptase mastermix [100 U SuperScript II (Invitrogen) ...
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bioRxiv - Developmental Biology 2023Quote: ... and RNase Inhibitor (0.25 U/µl; Promega). 25 µl of GFP-Trap beads (ProteinTech ...
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bioRxiv - Developmental Biology 2023Quote: ... and according to the manufacturer’s instructions (Dual-Glo Luciferase Assay System, Promega). One-way ANOVA with Tukey’s multiple comparisons tests were performed in GraphPad Prism version 8 to evaluate statistical significance.
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bioRxiv - Developmental Biology 2023Quote: ... Firefly and Renilla luciferase activities were measured 24 h after transfection with the Dual-Glo luciferase assay system (Promega) using a Luminometer (Berthold Technologies).
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bioRxiv - Developmental Biology 2023Quote: ... or pGEM-T (Promega) vectors and eight positive colonies/gene transferred to 750 ul of ddH2O and heated to 90 C to liberate the plasmids ...
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bioRxiv - Cell Biology 2023Quote: ... We then mixed the cell solution with 80 μL of NanoGlo luciferase assay reagent (Promega, Cat. No. N1110), transferred it to a 96-well plate well (PerkinElmer,Cat ...
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bioRxiv - Cell Biology 2023Quote: ... followed by dual-luciferase assay (Promega, E1960). Renilla luciferase (RL ...
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bioRxiv - Cell Biology 2023Quote: ... luciferase activities were measured using dual-luciferase assay (Promega, E1960). RL values were normalized against FL levels to determine the effect of the tethered protein on the expression of the target mRNA.
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bioRxiv - Evolutionary Biology 2023Quote: ... we prepared a 100 uL GoTaq (Promega, USA, product #M3001) polymerase chain reaction (PCR) ...
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bioRxiv - Cell Biology 2023Quote: ... we mixed 2500 ng of plasmid DNA with FuGene transfection reagent (Promega) and transfected 1 million Sf9 cells seeded in a 6 well plate ...
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bioRxiv - Cell Biology 2023Quote: NAD(P)+ and NAD(P)H concentrations were quantified with NAD(P)- and NAD(P)H-Glo assay kits (Promega) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... with 50 μl LARII (Luciferase Assay Reagent; Promega) and 25 μl Stop&Glo solution supplemented with the Stop&Glo substate (Promega).
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bioRxiv - Cell Biology 2023Quote: ... and lysed with 133 μl 1xPLB (Passive Lysis Buffer; Dual-Luciferase Reporter Assay System, Promega). 10 μl of each sample was analyzed using a GloMax Discover Microplate Reader (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... Dynabeads-Ab-Ag complexes were resuspended in 100 µl NH4HCO3 and digested by adding 0.2 μg of trypsin-LysC (Promega) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Subsequent digestion was performed with Trypsin/LysC solution [100ng/μl 1:1 Trypsin (Promega):LysC (FujiFilm ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with 49 ng of a TP1-Luciferase (Kurooka et al., 1998; Minoguchi et al., 1997) and 1 ng Renilla-Luciferase (pRL-TK, Promega) using Lipofectamine™ 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... 10 μl of each sample was analyzed using a GloMax Discover Microplate Reader (Promega) with 50 μl LARII (Luciferase Assay Reagent ...
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bioRxiv - Cell Biology 2023Quote: ... Cell viability was assessed by MTS using CellTiter 96® Aqueous One Solution Cell Proliferation Assay (Promega). For the transwell migration study ...
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bioRxiv - Cell Biology 2023Quote: ... Transfections were performed with FuGENE according to the manufacturer’s protocol (Promega; E2311). The cell culture medium was collected ...
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bioRxiv - Cell Biology 2023Quote: ... and Z-vad-fmk from Promega. No mycoplasma contamination confirmed by MycoBlue Mycoplasma Detector (Vazyme).
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bioRxiv - Cell Biology 2023Quote: ... Samples were then acidified with 12-21% aqueous phosphoric acid prior to digestion with Trypsin/Lys-C (Promega) and then added immediately to an S-Trap column (Protifi ...
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bioRxiv - Cell Biology 2023Quote: ... pHTC HaloTag® CMV-neo vector was purchased from Promega, pXT7-lyn-mKATE-E2A-phb-EGFP was kindly assembled by Frédéric Rosa.
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bioRxiv - Cell Biology 2023Quote: ... Dechorionated embryos were incubated with HaloTag 647 ligand (Promega) at 28°C for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... Cells expressing HaloTag were treated with JF646 HaloTag ligand (Promega; 1 μM final concentration in growth medium ...
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bioRxiv - Cell Biology 2023Quote: ... using the ECL Western Blotting Substrate (#W1015, Promega); or SuperSignal West Pico PLUS (#34577 ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were then subjected to digestion with LysC (Wako) at 37°C for 2h and trypsin (Promega) overnight at 37°C at a 1:50 enzyme:protein ratio ...
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bioRxiv - Cell Biology 2023Quote: ... cells were lysed using the Bright-Glo™ Luciferase Assay System (Promega). Subsequently ...