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Citations for Promega :
4701 - 4750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... in-gel digestion was performed on the PNS and enriched peroxisomal membranes using 300 ng trypsin (sequencing grade modified trypsin V5111; Promega) after reduction with 10 mmol/L dithiothreitol and alkylation with 55 mmol/L iodoacetamide proteins as described previously (Wolters et al. ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μl Oligo-dT 500 μg/ml (Promega), 0.2 μl Random hexamers 500 μg/ml (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... and PCR nucleotide mix (Promega, #U144B) were used to synthesise cDNA from 1 mg of RNA ...
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bioRxiv - Cell Biology 2023Quote: ... oligo (dT) 15 primers (Promega, #C1101), and PCR nucleotide mix (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... HaloTags were visualized with JaneliaFluor549 or 646-conjugated Halo Ligands (Promega; Madison, WI). Labeling of HaloTags was performed on live cells for 0.5-2 h at 30°C at a final concentration of 100 μM.
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bioRxiv - Cell Biology 2023Quote: ... and proteins were further digested by the addition of 2 μg trypsin (Promega #V5113) for 4 hours at room temperature on a shaker ...
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bioRxiv - Cell Biology 2023Quote: Constructs for expression in rabbit reticulocyte lysate (RRL) were based on the SP64 vector (Promega). For in vitro translations ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1 µL RNasin (Promega), 0.1 µL SP6 polymerase (New England Biolabs ...
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bioRxiv - Cell Biology 2023Quote: ... the PCR product was cloned into pGEMTeasy (Promega A1380). The mutagenesis of the EPIYA sites were done one after the other using the kit Quick change II site-directed mutagenesis (Aligent #200523) ...
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bioRxiv - Cell Biology 2023Quote: ... samples were proteolyzed with 0.5µg of Trypsin (Promega, sequencing grade) for 16h at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Three µl of 6X loading buffer (Promega, Madison, WI) were added to each reaction and samples were run on a 1% agarose-TAE gel ...
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bioRxiv - Cell Biology 2023Quote: ... Test proteins were generated in vitro using the TNR quick-coupled transcription-translation kit (Promega). For radiolabeling ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were treated with DNase post RNA extraction and purified using an RNA purification kit (Promega). RNA concentrations were assessed using a Nanodrop as well as a Qubit RNA analyzer for sample quality and RIN scores ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were then subjected to digestion with LysC (Wako) at 37°C for 2 hours and trypsin (Promega) overnight at 37°C at a 1:50 enzyme:protein ratio ...
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bioRxiv - Cell Biology 2023Quote: ... we generated pGEMT-col1a2 by cloning the zebrafish col1a2 sequence from a self-made zebrafish cDNA library into the pGEMT-easy vector using pGEM®-T Easy Vector Systems (Promega). Subsequently ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5 μg pMD2.G and 1.0 μg psPAX2 using Fugene HD (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... RNasin (Promega, #N251S), oligo (dT ...
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bioRxiv - Cell Biology 2023Quote: Genomic DNA was extracted using the Wizard Genomic DNA Purification Kit (Promega). Approximately 2×106 cells were lysed using nuclei lysis solution and mechanical trituration by pipetting ...
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bioRxiv - Cell Biology 2023Quote: ... the PCR product was cloned into pGEMTeasy (Promega A1380). The cagA fragment was digested with KpnI and EagI and subcloned into the lentivirus entry vector pEF-1α/pENTR (Addgene ...
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bioRxiv - Genomics 2023Quote: ... The reaction was stopped with the addition of 150 μL IP Elution Buffer (1% SDS, 0.1 M NaHCO3) and 2 μL Proteinase K (Promega, Cat # MC5005), then incubated at 65 °C overnight to reverse crosslinks ...
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bioRxiv - Developmental Biology 2023Quote: ... The obtained products were gel purified and cloned into the pGEM-T Easy Vector (Promega). Individual clones were sequenced and analyzed.
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with HaloTag TMR Ligand (Promega) at a final concentration of 100 nM for 30 min incubation ...
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bioRxiv - Cell Biology 2023Quote: ... the HaloTag-tagged SUV420H2 expression vector (FHC26822; Promega) was used as a template for PCR amplification of the target regions ...
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bioRxiv - Cell Biology 2023Quote: ... the two luciferases were measured sequentially using the dual luciferase assay kit (Promega Corp, Madison, WI, USA) and a BMG FLUOstar OPTIMA microplate reader ...
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bioRxiv - Cell Biology 2023Quote: Plasmids containing a promoter with binding sites for specific transcription factors to be measured driving firefly luciferase expression were co-transfected with a renilla luciferase reporter plasmid with a CMV promoter (Promega Corp, Madison, WI, USA) using TransIT reagent (0.25 µl/well ...
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Mitochondrial Apolipoprotein MIC26 is a metabolic rheostat regulating central cellular fuel pathwaysbioRxiv - Cell Biology 2023Quote: ... cDNA synthesis from 5 µg RNA was performed using the GoScriptTM Reverse Transcriptase Kit (Promega). Next ...
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Mitochondrial Apolipoprotein MIC26 is a metabolic rheostat regulating central cellular fuel pathwaysbioRxiv - Cell Biology 2023Quote: ... Cellular glucose uptake was measured using Glucose Uptake-GloTM Assay kit (Promega), according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... and were co-transfected with PB constructs (550 ng) and pBase plasmid (550 ng) using FuGENE HD Transfection (Promega E2311), following the protocol for reverse transfection ...
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bioRxiv - Cell Biology 2023Quote: ... After addition of 4 μl of RNAsin Plus ribonuclease inhibitor (Promega N2611), the samples were spun down ...
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bioRxiv - Cell Biology 2023Quote: ... followed by polyA enrichment using the PolyATtract® mRNA Isolation Systems (Promega). 9 μg of polyA RNA (1 μg/μl ...
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bioRxiv - Cell Biology 2023Quote: ... complete EDTA-free protease inhibitors and RNAsin (Promega N2611). Cells and allowed to swell by incubation at 4°C for 10 min and lysed with 20-25 strokes of a Dounce homogenizer ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were then subjected to proliferation assay using CellTiter 96® Non-Radioactive Cell Proliferation Assay (MTT) (Promega, Madison, USA) with protocol provided.
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bioRxiv - Developmental Biology 2023Quote: ... and the Reverse transcription kit (Promega) was used to make cDNA following manufacturer indications ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Extracted DNA was quantified on a Quantus flourometer (Promega, Maddison, WI, USA) using QuantiFlour dsDNA reagents ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the luciferase assay was performed using the dual luciferase reporter assay system (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we homogenized samples for 10 seconds in Maxwell RSC Homogenization Buffer (Promega, Madison, USA) with 2% 1-thioglycerol using a Bio-Gen PRO200 homogenizer (PRO Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and (iii) FEMTO Pulse genomic DNA analysis (Promega Corporation ...
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bioRxiv - Developmental Biology 2023Quote: ... 50 μg mL-1 Proteinase K (Promega V3021), both ...
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bioRxiv - Developmental Biology 2023Quote: ... The activity of luciferases was measured by the kit of Dual-Luciferase Reporter Assay System (Promega, E1960).
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bioRxiv - Evolutionary Biology 2023Quote: ... The BRh coding region was cloned into pGEM-T easy vector (Promega). A short oligonucleotide sequence encoding the 1D4 epitope of bovine rhodopsin (STTVSKTETSQVAPA ...
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bioRxiv - Cell Biology 2023Quote: ... 1.0 μg of RNA was reverse transcribed using random hexamer primers (Promega, Tokyo, Japan) and ImProm II reverse transcriptase (Promega) ...
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bioRxiv - Cell Biology 2023Quote: ... with psPAX2 and VSV-G plasmids using the FuGENE HD transfection reagent (Promega; E2311) to produce lentivirus ...
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bioRxiv - Cell Biology 2023Quote: The HaloTag-tagged KDM4D expression vector (FHC06842; Promega), the PB533-based H2B-Halo expression vector (Uchino et al. ...
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bioRxiv - Cell Biology 2023Quote: ... plated in a 24-well glass-bottom plate were incubated with Janelia Fluor 646 HaloTag Ligand (Promega) for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... FuGENE HD (Promega) was used according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... 50 ng of random primers (Promega, C118A), 0.5 mM dNTPs (NEB ...
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bioRxiv - Evolutionary Biology 2023Quote: ... with 2% 1-thioglycerol using a Bio-Gen PRO200 homogenizer (PRO Scientific, Oxford, USA) and then extracted RNA using the Maxwell RSC simplyRNA Tissue Kit (Promega). We quantified extracts using a Quant-IT RNA Assay Kit ...
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bioRxiv - Microbiology 2023Quote: ... and FuGENE HD (Promega) reagent respectively ...
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bioRxiv - Cell Biology 2023Quote: ... 2013) and purified using either endotoxin free mini-preps or midi-preps according to manufacturer protocol (Promega, Madison, WI). Repair cassettes were amplified using primers designed to be homologous to the C-terminal genomic DNA surrounding the STOP codon ...
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bioRxiv - Cell Biology 2023Quote: ... the TUNEL assay was performed using the DeadEnd Fluorometric TUNEL System (Promega, USA) as per the manufacturer’s instructions ...