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Citations for Promega :
4751 - 4800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Cellular ATP levels were quantified using the CellTiter-Glo® assay (Promega, G7570). The reagent was mixed at a 1:1 ratio with supernatant from the treatment plate ...
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bioRxiv - Developmental Biology 2023Quote: ... 10 U μl-1 RNAsin (Promega), 1Å∼ Superscript II First-Strand Buffer ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 500 ng of RNA were reverse-transcribed using M-MLV Reverse Transcriptase and Random Hexamer primers (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The cells that remained attached were analyzed with the CellTiter-Glo Luminescent Cell Viability Assay (Promega).
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bioRxiv - Cell Biology 2023Quote: ... and then 4 hours in mass-spectrometry-grade trypsin (Promega). The missed cleavage rate for samples was an average of 3.9%-21.9% ...
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bioRxiv - Developmental Biology 2023Quote: ... 16/32-cell embryos were co-injected with 5 pg of pRL-TK:renilla luciferase plasmid (Promega E2241) + 80 pg of the pGL4.23 luc2/miniP HumanPDX1 enhancer:firefly luciferase plasmid (wt or 6RFX motif mutant ...
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bioRxiv - Developmental Biology 2023Quote: ... and cloned into the pGL4.23 firefly luc2/miniP vector (Promega E8411). 16/32-cell embryos were co-injected with 5 pg of pRL-TK:renilla luciferase plasmid (Promega E2241 ...
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bioRxiv - Developmental Biology 2023Quote: ... or pGEM-T vector system (Promega®), and sequences were validated by Sanger sequencing ...
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bioRxiv - Developmental Biology 2023Quote: Carm1 promoter region (-630 to +15) was cloned to pGL4 luciferase reporter vector (Promega) to be the Carm1-reporter ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested with 1 μg of lysyl endopeptidase (Wako) at room temperature overnight and were further digested overnight at room temperature with 1 μg trypsin (Promega). The resulting peptides were desalted with an HLB column (Waters ...
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bioRxiv - Genetics 2023Quote: Extracted genomic DNA from HLA-iPSCs and PBMCs was analyzed for 24 polymorphic STR markers using GenePrint 24 system (Promega, Madison, USA) following manufacturer’s protocol and were amplified using PCR and followed by ABI capillary electrophoresis ...
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bioRxiv - Genetics 2023Quote: ... PCR products were purified using the Wizard SV Gel and PCR Clean-Up System (Promega) in line with the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Lysates were then incubated with Luciferase substrate (Promega), and luciferase activity was measured for 5s at a Tecan Infinity luminometer.
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bioRxiv - Immunology 2023Quote: ... tailed with complete TRAC and TRBC1 gene segments amplified from human genomic DNA (Promega), respectively ...
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bioRxiv - Cell Biology 2023Quote: ... followed by trypsin (Promega, V5111) at a 1:50 (enzyme:protein ...
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bioRxiv - Cell Biology 2023Quote: Nanoluc activity was measured using NanoGlo luciferase assay reagents (Promega N1150) following manufacture’s instruction ...
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bioRxiv - Cell Biology 2023Quote: ... RLU reading was performed with a microplate reader (Promega Glomax Multi Detection) using the luminescence protocol.
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bioRxiv - Cell Biology 2023Quote: ... HaloTag7 (G1891; Promega), 3×FLAG ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were collected and analyzed using a Luciferase Assay System (Promega). Luminescence was measured using a Varioskan Flash (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... The amplified fragments were subcloned into pGEM-T Easy Vector (Promega). The digested inserts were labeled with [α-32P] dCTP and hybridized individually onto nylon membranes (Hidema et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Fixed cells were permeabilized using 0.5% Triton X-100 (Promega, Cat#H5142). Primary antibodies were added at 1:250 dilution in 1X Animal-Free Blocking Solution (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2023Quote: ... into the pGEM-T vector (Promega A1360). The dCdk8-EGFP rescue construct ...
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bioRxiv - Cell Biology 2023Quote: ... HaloTag (Promega, N2701), OMP2529 (the signal sequence for mitochondria outer membrane targeting ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting supernatant was diluted at 1:50 with PBS and used for luminescence measurement with the Nano-Glo Luciferase Assay System (cat. #N1110, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... freshly added protease inhibitor cocktail 1x (Promega)) ...
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bioRxiv - Immunology 2023Quote: Cells in a 96 well plate were lysed with Glo-Lysis buffer (Promega, 50 µl) and 25 µl cell lysates were mixed with 25 µl Bright-Glo or Nano-Glo (both Promega) ...
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bioRxiv - Immunology 2023Quote: ... Four digestion strategies were employed: digestion with Trypsin (Tryp) only (Trypsin Gold, Mass Spectrometry Grade, Promega Cat 5280), Chymotrypsin (Ct ...
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bioRxiv - Immunology 2023Quote: ... and AspN (AspN, Sequencing Grade, Promega Cat V1621).
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bioRxiv - Immunology 2023Quote: ... Commercially available luciferase reagent (Bright-Glo; Promega, Madison, WI) was added and luminescence was measured using the Synergy LX multilabel plate reader (Agilent ...
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bioRxiv - Immunology 2023Quote: ... Cellular viability was measured using Cell Titer-Glo (Promega). Secondary screen for IFNB1 mRNA induction was performed similarly in 293T cells seeded in 48-well plates ...
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bioRxiv - Immunology 2023Quote: ... and then resuspended in 30 µL PNK dephosphorylation mix (1X PNK buffer (Promega), 0.5 µL RNaseIN ...
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bioRxiv - Immunology 2023Quote: ... equal amounts (∼500 µg) of each sample were treated with RNaseIN as control (Promega, 40 U), or an RNase cocktail (per sample ...
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bioRxiv - Immunology 2023Quote: ... 1 µL T4 PNK (Promega), 4 µL PEG400) ...
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bioRxiv - Immunology 2023Quote: was done using the CellTiter Glo Kit (Promega).
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bioRxiv - Immunology 2023Quote: ... the indicated plasmids were mixed with an empty vector to a total of 2.0 μg DNA in 125 μL Opti-MEM and transfected using FuGENE HD (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... This was followed by addition of 1 μL M-MLV RT (Promega) to initiate reverse transcription in a 20 μL reaction of the M-MLV buffer ...
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bioRxiv - Genomics 2023Quote: ... purified Homo sapiens DNA (Promega, Madison, WI) with a microbial community standard (Zymo Research ...
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bioRxiv - Immunology 2023Quote: ... Cells were then lysed and Firefly and Renilla luciferase activities were determined with the Dual-Luciferase Reporter Assay System (Promega) and an EnVision plate reader (PerkinElmer ...
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bioRxiv - Immunology 2023Quote: ... after intraperitoneal luciferin (Promega, Madison, WI) injections according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Luciferase activity was detected using BrightGlo (Promega, Madison, WI). Measured relative light units (RLU ...
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bioRxiv - Immunology 2023Quote: ... and cDNA was synthesized using M-MLV reverse transcriptase (Promega, Madison, WI). Real time quantitative PCR analysis was performed using a QuantStudio 3 real time PCR system (Applied Biosystems ...
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bioRxiv - Genetics 2023Quote: ... then diluted to 1M urea and digested with trypsin (Promega, 1:50 w/w) overnight at 37°C ...
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bioRxiv - Genetics 2023Quote: ... gDNA was removed from RNA samples using RQ1 DNAse (Promega, Madison, Wisconsin). mRNA-seq libraries were prepared using 50-100 ng of RNA (with a RIN score greater than five) ...
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bioRxiv - Genetics 2023Quote: ... 65µL of Luciferase Assay Reagent (Promega) was added in unison to the lysates and luminescence activity in counts per second were immediately recorded on a Victor X4 plate reader (Perkin Elmer) ...
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bioRxiv - Genetics 2023Quote: ... and 0.45% Tween-20) with Proteinase K (20mg/ml, Promega, Cat. No. MC5005) added just before use ...
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bioRxiv - Genetics 2023Quote: ... This fragment was cloned into the pGL3 luciferase reporter vector (Promega) by standard cloning methods ...
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bioRxiv - Genetics 2023Quote: Forty-eight hours after transfection the cells were washed once with 0.5mL sterile PBS and 75uL of 1x Reporter Lysis Buffer (Promega) supplemented with protease inhibitor cocktail lacking EDTA (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... 50 µL of ATDC5 Complete Media and 10 µL of CellTiter 96 AQueous One Solution (Promega G3580) were added to each well ...
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bioRxiv - Genetics 2023Quote: ... Additional 140 uL of lysis buffer and 40uL of proteinase K (20mg/ml, Promega, Cat. No. MC5005) were added ...
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bioRxiv - Genetics 2023Quote: ... 35µL of 1x β-galactosidase assay buffer (Promega) was added in unison to plated lysates and mixtures were incubated at 37°C for 40 min ...