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Citations for Promega :
4651 - 4700 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Cell viability was assayed with CellTiter-Glo® (Promega) or Alamar Blue (resazurin).
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bioRxiv - Cell Biology 2023Quote: The activity of the proteasome’s β5 sites was determined either by Succinyl(Suc)-LLVY-AMC (7-amido-4-methylcoumarin) fluorogenic substrate or by the Proteasome-Glo™ assay (Promega), a luciferase coupled assay ...
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bioRxiv - Biophysics 2023Quote: ... and FuGENE 6 transfection reagent (Promega, USA), propagated ...
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bioRxiv - Cell Biology 2023Quote: HaloTag-Ligand-Amine (Promega, #P6741) was reconstituted in DMF (Sigma-Aldrich ...
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bioRxiv - Genomics 2023Quote: ... according to manufacturer protocol and a RNAse inhibitor mix (0.04 U/µl SUPERaseIN RNAse Inhibitor [AM 2696, Thermo Fisher]; 0.04 U/µl RNAsin Plus RNAse Inhibitor [N2615, Promega]) was added ...
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bioRxiv - Neuroscience 2023Quote: ... These reactions were assembled to a final volume of 20 μL using 5X Green GoTaq® Reaction Buffer and dNTPs (Promega) with laboratory-purified DNA Taq Polymerase ...
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bioRxiv - Genetics 2023Quote: ... Eluted proteins were digested with sequencing-grade trypsin (Promega). Peptide mixtures were separated using Evosep One (Evosep Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... before the bands corresponding to the homozygous amplicon size were excised and purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, Madison, Wisconsin, USA) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... All PCR reactions were performed using GoTaq Green Master Mix (Promega, M7123) with 0.25 ng total cDNA per reaction ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Comparison of VUF15485-induced β-arrestin2 recruitment to human and mouse ACKR3 was also measured by NanoLuc complementation assay (NanoBiT, Promega Corporation, Madison, WI, USA) in HEK293T cells (Dixon et al. ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cells were pre-incubated in Hanks’ balanced salt solution with increasing concentrations of compound for 60 minutes before stimulation with 10 nM CXCL12 and addition of 2 µM Renilla Luciferase substrate coelenterazine-h (Promega). After 20 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were then digested with 0.1 μg trypsin (Promega) at 37°C overnight ...
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bioRxiv - Microbiology 2023Quote: ... proteins were digested overnight with 0.63 µg trypsin (Promega). Tryptic digestion was stopped by adding formic acid to a final concentration of 1.8% (v/v) ...
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bioRxiv - Immunology 2023Quote: ... followed by goat anti-mouse immunoglobulin conjugated to alkaline phosphatase (Promega S3721, dilution 1:7500). Focuses of infection were revealed using NBT/BCIP reagent (Promega) ...
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bioRxiv - Immunology 2023Quote: ... Transfections were performed with FuGene6 (Promega), following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... and yields were quantified using with the QuantiFluor dsDNA system (Promega, cat#E2670) in a 96-well microplate format.
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bioRxiv - Plant Biology 2023Quote: ... 500 ng-1 µg of the purified template DNA library was used for the in vitro transcription reaction (T7 RiboMAX™ Express Large Scale RNA Production System, Promega). Unincorporated nucleotides were removed using mini Quick Spin RNA Columns (Roche) ...
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bioRxiv - Plant Biology 2023Quote: ... were performed using GoTaq G2 Green Master Mix (Promega, cat#M7822). Secondary validation of genotyping reactions was performed as needed using the Quick-DNA Plant/Seed Miniprep kit (Zymo Research ...
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bioRxiv - Microbiology 2023Quote: ... The Veritas Microplate Luminometer (Promega) was used to measure and record luminescence.
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bioRxiv - Microbiology 2023Quote: ... and random 9-mers in the presence of RNasin (Promega). qPCR was then performed on a Quantstudio 3 using the indicated targets and with iTaq Universal SYBR Green (BioRad) ...
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bioRxiv - Microbiology 2023Quote: ... cells were reactivated in the presence of 10 nM JF646 Haloligand (Promega) with doxycycline and sodium butyrate as above ...
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bioRxiv - Microbiology 2023Quote: ... and reverse transcribed using AMV RT (Promega) and random 9-mers in the presence of RNasin (Promega) ...
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Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55BbioRxiv - Neuroscience 2023Quote: ... On-column digestion was performed by incubating 60 µL (1.5 µg) Trypsin/Lys-C Mix (Mass Spec Grade; Promega, UK) in 50 mM TEABC solution at pH 8 on the samples on a thermomixer at 47°C for 1 hour before reducing the incubation temperature to 22°C for overnight digestion ...
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bioRxiv - Immunology 2023Quote: ... Quantitative PCRs (qPCRs) were performed with GoTaq ® qPCR Master mix (Promega) following the standard cycling conditions suggested by the manufacturer in a StepOnePlus real time PCR system (Applied Biosystems) ...
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bioRxiv - Cell Biology 2023Quote: ... The cell pellet was resuspended in 100 μl fresh TDB and HaloTag ligand (either in-house labelled Alexa647 ligand or Halo-Tetramethylrhodamine (TMR, Promega, USA) was added to a final concentration of 500 nM (Alexa647 ...
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bioRxiv - Cell Biology 2023Quote: ... The Halo tag sequence was amplified from the pFC20A (HaloTag®) T7 SP6 Flexi® vector (Promega, USA). All amplified fragments were then combined using Gibson assembly (Gibson assembly cloning kit ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: β-arrestin2 recruitment to human chemokine receptors in response to 1 µM VUF15485 or 200 nM positive control chemokines was also monitored by NanoLuc complementation assay (NanoBiT, Promega) (Dixon et al. ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lines were transfected with plasmids using Viafect (Promega) in 6 well plates according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription was performed using AMV RT (Promega) in the presence of RNasin (Promega) ...
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bioRxiv - Plant Biology 2023Quote: ... Plasmid pFL61 harbouring the construct RiSKC3-GFP under the control of the PGK promoter was purified from StellarTM bacteria using the PureYield™ Plasmid Miniprep System (Promega). The yeast transformation protocol was described by (50).
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bioRxiv - Plant Biology 2023Quote: ... DNA fragments were excised from the gel by means of x-tracta™ Gel Extractor (Promega) and purified using Wizard® SV Gel and PCR Clean-Up System (Promega) ...
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bioRxiv - Cancer Biology 2023Quote: ... and luciferase assays were performed using a dual luciferase assay kit (Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55BbioRxiv - Neuroscience 2023Quote: ... or HaloLink Resin (Promega) for 1-2 h (20 µl of packed resin/ 1 mg of lysate) ...
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bioRxiv - Cell Biology 2023Quote: ... Janelia Fluor (JF) Ligand-647 (Promega) was added to the imaging media at a final concentration of 75nM ...
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bioRxiv - Biochemistry 2023Quote: ... Zc3h18-3F-mAID mES cells were transfected with pB-MYC-mZC3H18x-BSD vectors along with a piggyBAC transposase expressing vector (pBase) in a 1:1 ratio using Viafect (Promega). Cell pools were selected with BSD for ∼ 7-10 days or until negative control cells no longer survived ...
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bioRxiv - Physiology 2023Quote: ... The GoTaq Green Master Mix (Promega, M7123) was used for PCR genotyping ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then unfixed overnight in 150 mM NaCL and 60C and 50 ug/mL protease K (Promega). DNA was then extracted using a single column of QIAamp DNA Blood Mini Kit (Qiagen) ...
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bioRxiv - Microbiology 2023Quote: ... in the presence of RNasin (Promega). qPCR was performed with iTaq Universal SYBR Green (BioRad ...
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bioRxiv - Microbiology 2023Quote: ... 10 nM JF646 Haloligand (Promega). 24 hours post reactivation ...
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bioRxiv - Immunology 2023Quote: ... Double stranded RNA (dsRNA) was synthesized from purified T7-tagged PCR amplicons using the T7 RiboMax Express Large-Scale RNA production system (Promega) according to the manufacturer’s instructions and purified as previously described [33] ...
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Treatment with furosemide indirectly increases inhibitory transmission in the developing hippocampusbioRxiv - Neuroscience 2023Quote: ... blots were incubated with Enhanced luminol-based Chemiluminescence substrate (Promega), and the exposure was captured using the ImageQuant 800 system (Amersham) ...
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bioRxiv - Neuroscience 2023Quote: The PCR fragment were ligated into a pGEM-T Easy Vector System (Promega), transformed into chemically competent cells and sequenced ...
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bioRxiv - Molecular Biology 2023Quote: ... 1ng Luciferase Spike-In RNA (Promega), and 1X protease inhibitor cocktail ...
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bioRxiv - Cell Biology 2023Quote: ... proteinase K digestions were stopped by 10 mM phenylmethanesulfonyl fluoride (final concentration) (Protease Inhibitor Cocktail, Promega) on ice for 20 min ...
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bioRxiv - Biochemistry 2023Quote: ... The gel pieces were saturated with 250 ng trypsin (Promega) in 50 μL 10 mM ammonium bicarbonate ...
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bioRxiv - Immunology 2023Quote: ... Renilla luciferase internal control vector pRL-TK and the dual luciferase reporter assay kit were from Promega (Madison, WI). Phusion PCR enzyme ...
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bioRxiv - Plant Biology 2023Quote: ... 10 µL of 5× Green GoTaq® Reaction Buffer (Promega), 1 µL of dNTP Mix (10 mM each of dTTP ...
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bioRxiv - Plant Biology 2023Quote: ... 0.25 µL of GoTaq® DNA Polymerase (5 U/µL, Promega) and 28.25 µL of ultra-pure water were added into the tubes ...
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bioRxiv - Plant Biology 2023Quote: ... 0.25 µL of GoTaq® DNA Polymerase (5 U/µL, Promega), and 27.75 µL of ultra-pure water ...
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bioRxiv - Plant Biology 2023Quote: ... 10 µL of 5× Colorless GoTaq® Reaction Buffer (Promega), 1 µL of dNTP Mix (10 mM each of dTTP ...