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Citations for Promega :
3151 - 3200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... CellTiter 96 aqueous one solution cell proliferation assay kit was from Promega (Madison, USA).
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... Infectivity was determined after 3 days by luminescence using a Promega GloMax plate reader following addition of BrightGlo reagent (Promega #E2620) to Calu-3 cells or FLuc Assay buffer (Nanolight #318 ...
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bioRxiv - Microbiology 2024Quote: ... Luminescence (relative luminesce units, RLU) and Absorbance (OD595) of sample triplicates supplemented with NanoGlo Live Cell Assay System (Promega) as recommended by the manufacturer ...
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bioRxiv - Microbiology 2024Quote: ... rabbit anti-Halo (1:300, Promega), rabbit anti-H3K9me3 (1:300 Abcam ...
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bioRxiv - Microbiology 2024Quote: ... colonoids were imaged with phase-contrast light microscopy (Keyence BZ-X800) for counting or lysed for viability assays using CellTiter-Glo 3D (Promega) and a luminometer (Bio-Tek Synergy HTX).
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bioRxiv - Microbiology 2024Quote: ... Nanoluciferase-based bioluminescence resonance energy transfer (BRET) was measured using NanoBRET™ Nano-Glo® Substrate on a GloMax® Discover Multimode Microplate Reader (Promega).
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bioRxiv - Biochemistry 2024Quote: ... 100 μL of culture supernatant was removed from each well and replaced with 100 μL of ONE-Glo luciferase reagent (Promega, Madison, WI, Cat# E6110). The plates were gently mixed by rocking for 10 min at rt ...
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bioRxiv - Microbiology 2024Quote: ... and RNase ONE Ribonuclease (Promega) to deplete host and bacterial nucleic acid ...
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bioRxiv - Bioengineering 2024Quote: ... and the luminescence was read on a luminometer (Promega) after 100 µL of luciferin solution (Promega ...
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bioRxiv - Bioengineering 2024Quote: ... after 100 µL of luciferin solution (Promega) was added well by well by an autosampler ...
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bioRxiv - Microbiology 2024Quote: ... USA) and 10 μl of GoTaq 1-Step RT-qPCR (Promega, Madison, WI, USA). Thermal cycling was performed at 50°C for 15 min for reverse transcription ...
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bioRxiv - Microbiology 2024Quote: ... following manufacturer’s instruction (Promega, Wisconsin, USA). The G-Luc values were normalized to that of cell viability and plotted as mean (+ SD) ...
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bioRxiv - Microbiology 2024Quote: ... Cell viability was measured using a commercially available kit [CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega, Madison, USA)] ...
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bioRxiv - Microbiology 2024Quote: ... Recombinant pNZ8037 plasmid carrying wild-type or mutated ptnCD genes was isolated using PureYieldTM Plasmid Mini-prep System (Promega, USA). Targeted changes in the ptnCD genes were introduced following the Site Directed Mutagenesis Cornell iGEM 2012 Protocol (http://2012.igem.org/wiki/images/a/a5/Site_Directed_Mutagenesis.pdf) ...
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bioRxiv - Neuroscience 2024Quote: HEK239T cells were transfected using Fugene6 (Promega) with a DNA-to-Fugene ratio of 1:3 for 18-24 hours.
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bioRxiv - Evolutionary Biology 2024Quote: ... while gene targets were cloned into psi-check2 vector (Promega). All constructs were sequenced to confirm their identities ...
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bioRxiv - Microbiology 2024Quote: Cells or tissues were lysed with Passive Lysis Buffer (Promega, USA) before centrifugation for 10 minutes at 13,000g followed by a ten times dilution in Phosphate-buffered saline (PBS) ...
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bioRxiv - Bioengineering 2024Quote: ... Proteins were subsequently digested with trypsin (sequencing grade, Promega, Madison, WI), at an enzyme/substrate ratio of 1:50 ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA was extracted from bacterial cells grown overnight in MOKA-rich medium using the Wizard genomic DNA purification kit (Promega) or as described (62 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were processed at different times post inoculation (pi) according to manufacturer’s instructions (Caspase-Glo® 3/7 Assay kit, Promega, USA) to determine caspase 3/7 activity ...
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bioRxiv - Microbiology 2024Quote: ... 1 µg trypsin (Promega, Fitchburg, USA) was used to digest 50 μg of total solubilized protein from each sample ...
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bioRxiv - Immunology 2024Quote: ... qRT-PCR was performed in triplicate using SYBR Green Master Mix (Promega, US) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... After dehydration enough trypsin (6ng/ul, Promega Sequencing grade) was added to just cover the gel pieces and the digestion was allowed to proceed overnight (∼16 hrs. ...
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bioRxiv - Bioengineering 2024Quote: ... after 100 µL of luciferin solution (Promega) was added well by well by an autosampler ...
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bioRxiv - Bioengineering 2024Quote: ... 20 µL of undiluted sample was loaded onto a white 96 well-plate and the luminescence was read on a luminometer (Promega) after 100 µL of luciferin solution (Promega ...
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bioRxiv - Microbiology 2024Quote: In vitro toxicity was assessed in HeLa cells (ATCC® CCL-2) using the ApoTox-GloTM Triplex Assay (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... luminescence activity was analyzed by the Nano-Glo® HiBiT Lytic Detection System (Promega). Based on the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Cells were lysed and luciferase activity was measured using the DualLuciferase reporter assay system (Promega) on a Cytation 5 Multi-Mode Reader (BioTek ...
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bioRxiv - Microbiology 2024Quote: ... CellTiter-Glo® 2.0 Cell Viability Assay (Promega) was used to investigate the cytotoxicity of AEVs on THP1-Blue cells ...
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bioRxiv - Microbiology 2024Quote: ... The sample was processed according to the SP3 protocol36 and digested overnight with trypsin (Promega, enzyme/protein 1:50). Peptides were desalted using SBD-RPS tips as previously described37 ...
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bioRxiv - Genetics 2024Quote: ... The reaction was performed in a 50-μL mixture containing 1.25 U GoTaq DNA polymerase (Promega), 200 μM dNTPs ...
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bioRxiv - Developmental Biology 2024Quote: A DeadEnd Colorimetric TUNEL assay kit (Promega - G7360) was used to detect apoptosis in Hmgcr knockdown cells ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were digested with 1.5 µg of trypsin (Promega) at 47°C for 2h ...
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bioRxiv - Genetics 2024Quote: ... 13ng of pCMV-VP16 (EV) or pCMV-LXRα-VP16 and 13 ng of GAL4-Co-factor vector and 13ng of pRL-TK (Promega, 2241) using Lipofectamine 3000 ...
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bioRxiv - Developmental Biology 2024Quote: ... COL1A1 and THBS2 probes were generated using a Roboprobe kit (Promega; ref P1460), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and reverse transcription (RT) was performed using the M-MLV Reverse Transcriptase (Promega). The genomic DNA of mouse tissue was extracted using DNeasy Blood & Tissue Kit (Qiagen) ...
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bioRxiv - Bioengineering 2024Quote: ... 1 µg of total RNA was reverse-transcribed at 42 °C for 90 min and 72 °C for 15 min using the moloney murine leukemia virus (M-MLV) reverse transcriptase (Promega). Each cDNA sample was diluted 8-folds and used for qRT-PCR with the specific primer pairs of each gene listed in Supplementary Table 1 ...
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bioRxiv - Microbiology 2024Quote: ... cells were lysed and subjected to luciferase assays (Promega). Results were expressed as NLRs calculated as follows ...
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bioRxiv - Molecular Biology 2024Quote: ... cell viability is measured using CellTiter Fluor (Promega) and cellular luciferase is measured using Quanti-Luc ...
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bioRxiv - Molecular Biology 2024Quote: ... samples were incubated with 80 μL of 2 M urea in 50 mM Tris-HCl (pH 7.5) containing 1 mM DTT and 0.5 μg trypsin (Promega) at 25°C with shaking for pre-digestion ...
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bioRxiv - Molecular Biology 2024Quote: ... equal amounts of (strand specific) cDNA was used with Ready Go Taq Polymerase (Promega).
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bioRxiv - Molecular Biology 2024Quote: ... and 1 U/μl RNAsin Plus (Promega) and flash frozen in LN2 (Hopes et al ...
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bioRxiv - Neuroscience 2024Quote: ... Synthesis of cDNA was performed using the ImProm-IITM Reverse Transcription System (Promega). Primers used were as follow ...
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bioRxiv - Molecular Biology 2024Quote: ... The GoTaq qPCR Master Mix (Promega, Madison, USA) was used following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... TFs mRNA levels were evaluated by reverse transcription followed by real-time quantitative PCR (RT-qPCR) using the GoTaq 1-Step RT-qPCR System (Promega, Madison, USA) and primers listed in S5 Table ...
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bioRxiv - Molecular Biology 2024Quote: ... Equal amounts of cDNA was used with Ready Go Taq Polymerase (Promega). To ensure specific DNA was amplified ...
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bioRxiv - Molecular Biology 2024Quote: ... an extra 1-hour room temperature incubation in 1:2500 anti-Mouse IgG-HRP secondary antibody (Promega, W4021) + 1% BSA + TBS was performed prior to Clarity Western ECL detection.
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bioRxiv - Molecular Biology 2024Quote: ... The renilla and firefly luciferase activities were measured using Dual-Glo Luciferase Assay System (Promega) after 16 h of transfection ...
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bioRxiv - Microbiology 2024Quote: ... The luciferase activity of cell lysates was measured using a firefly luciferase assay kit (Promega, USA), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The cells were lysed with lysis reagent (Promega) and the cell lysate was collected ...