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Citations for Promega :
3051 - 3100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The urea concentration in the lysate was reduced to 2 M with the addition of 50 mM NH4HCO3 and the samples were subjected to overnight trypsin digestion (Trypsin Gold, MS Grade, Promega Corporation, WI, USA). Following digestion ...
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bioRxiv - Plant Biology 2024Quote: ... adapted ReliaPrep RNA extraction kit (Promega, Z6012) and subjected to quality control ...
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bioRxiv - Pathology 2024Quote: ... DNA was extracted from tails and/or kidneys using Maxwell DNA purification kits (Cat # AS1120; Promega). The information of primers for PCR is shown in Supplemental Table I ...
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bioRxiv - Pathology 2024Quote: Total RNA was extracted from kidneys using Maxwell® RSC simplyRNA Tissue Kits (Cat # AS1340; Promega) in accordance with the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... The RNA samples were treated with DNAse (RQ1 Rnasa-Free Dnase, Promega, Madison, WS, USA) and retrotranscribed with FireScript KIT Reverse Transcriptase (Solis BioDyne ...
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bioRxiv - Cell Biology 2024Quote: TUNEL staining was performed as previously described using the DeadEnd kit (Promega, Madison, WI, USA, Cat # G3250) (Wubben et al. ...
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bioRxiv - Immunology 2024Quote: ... and RNasin® Ribonuclease Inhibitor (#N2615, Promega; 1:1000)) ...
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bioRxiv - Microbiology 2024Quote: ... (Promega) 0.4 mM dNTP Mix (Promega) ...
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bioRxiv - Microbiology 2024Quote: ... 0.4 mM dNTP Mix (Promega), 4 μM of each primer (final concentrations) ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... digested with sequencing-grade trypsin (Promega, V5113), and eluted according to manufacturing protocols ...
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bioRxiv - Biochemistry 2024Quote: ... The well plates were incubated at 30 °C and the optical density at 560 nm was measured using a plate reader (Promega ProMax). We defined a growth inhibition of at least 50 % in comparison to the growth in the DMSO-supplemented medium as the cut-off to determine the MIC.
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bioRxiv - Biophysics 2024Quote: ... The luciferase activities were measured by using a ONE-Glo Luciferase Assay System (Promega, Madison, WI) and an Ensight Multimode Plate Reader (PerkinElmer ...
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bioRxiv - Biophysics 2024Quote: ... in which the interaction between a Gα subunit and a Gβγ subunit was monitored by the NanoBiT system (Promega). Specifically ...
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bioRxiv - Biophysics 2024Quote: ... Proteins were digested to peptides by adding 25 µL of 50 mM ammonium bicarbonate containing 5 µg of trypsin (Promega) and incubating at 37 °C for 18 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... Cellular viability was determined using CellTiter-Glo assay (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: ... The next day cells were treated with compounds and assessed for cell growth 5 days later using CellTiterGlo (Promega).
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bioRxiv - Cancer Biology 2024Quote: ... Firefly luciferase activity was measured via dual luciferase assay reporter system (Promega) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... 100 µg/mL RNAse A (Promega)) for 2 h at RT ...
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bioRxiv - Developmental Biology 2024Quote: The efficacy of all MOs was verified in western blots following in vitro transcription and translation (TNT-coupled Wheat Germ Extract kit, Promega) of pCMV-Sport6-marcks.S ...
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bioRxiv - Developmental Biology 2024Quote: ... PVDF membranes were subsequently incubated with Streptavidin Alkaline Phosphatase (1:5000; Promega) to visualize proteins in Transcend Chemiluminescence Substrate (Promega) ...
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bioRxiv - Cell Biology 2024Quote: ... Protein lysates from the single cells were digested with 2 ng of Trypsin (Promega, Cat. V5280), dissolved in 1 μl of 100 mM TEAB pH 8.5 containing Benzonase (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... using FuGene® HD (Promega, PR-E2311) transfection reagent ...
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bioRxiv - Cancer Biology 2024Quote: ... RealTime Glo (Promega) cell viability assay was used to compare unstimulated and stimulated T-cells as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and 100uL of Bright-Glo luciferase assay reagent (Promega PRE2620) was added according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were was lysed and Cell lysates were subjected to dual reporter luciferase assays according to the manufacturer’s instructions (Promega).
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bioRxiv - Cancer Biology 2024Quote: ... The plate was then left at room temperature for 7 min before reading luminescence according to the BrightGlo manufacturer’s instructions (Promega PRE2620 ...
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bioRxiv - Cancer Biology 2024Quote: ... 800bp or 300bp human HNF4α promoter into pGL3 basic vector (Promega) between XhoI and HindIII ...
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bioRxiv - Cancer Biology 2024Quote: ... CellTiter Glo (Promega) was used to measure ATP levels on days 0 ...
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bioRxiv - Cancer Biology 2024Quote: ... Apoptosis was measured similarly by determining active caspases 3/7 via CaspaseGlo 3/7 (Promega) on day 3 after plating ...
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bioRxiv - Developmental Biology 2024Quote: ... the samples were further diluted to 2 M urea with 100 mM TEAB and trypsin (Trypsin Glod, Promega) was added at a ratio of 1:50 and the samples were incubated at 37°C over night ...
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bioRxiv - Cell Biology 2024Quote: ... 2 μL of 50% Cell-Titer Glo (Promega No G7573) diluted in water is added to the cells and luminescence measured on an EnVision Plate Reader (Perkin Elmer)
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bioRxiv - Developmental Biology 2024Quote: ... pGL3-PDE6B-146-luc was produced by amplifying a 146 bp promoter region of PDE6B (81) from H9 hESC genomic DNA and inserting into a pGL3-SV40 (Promega, primers #7,8), then removing the SV40 promoter (primers #9,10 ...
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bioRxiv - Cell Biology 2024Quote: ... the Qubit 2.0 using the Promega Quantifluor ONE kit (Promega, WI, USA) and the AATI Fragment Analyzer (Agilent Technologies Inc. ...
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bioRxiv - Cell Biology 2024Quote: ... and 250 ng of mass spectrometry grade trypsin (Promega, V528A) was added ...
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bioRxiv - Cell Biology 2024Quote: ... 5 regions of ieCTNNB1 and the region containing the mutation site were respectively cloned into pGL3- promoter vector (Promega). HEK293T ...
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bioRxiv - Cell Biology 2024Quote: ... Promega Dual Glo test kit (Promega, E1910) was used to measure luciferase activity according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... BP1425-500) at 37 °C and the plasmid was isolated from single colonies using the PureYield Plasmid Midiprep kit (Promega, Cat no. A2495), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... precipitated proteins were resuspended in 0.1% RapiGest with 6 μL of sequencing-grade trypsin (Promega, 0.5 μg/μL) added to each sample and digested overnight at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... Triglycerides were quantified by a luciferase-based assay (Promega, J3160)
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bioRxiv - Cancer Biology 2024Quote: ... 6X Cell Titer Blue solution (Promega Corporation, Madison, WI) was added into wells with cells and wells with plain media as a no cell control ...
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Tumor microenvironment acidosis favors pancreatic cancer stem cell properties and in vivo metastasisbioRxiv - Cancer Biology 2024Quote: ... lysates were acquired with Passive Lysis Buffer (Promega, #E1910), diluted in ddH2O ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal volume of Cell-Titer Glo Reagent (Cell-Titer Glo kit, Promega) was added ...
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bioRxiv - Cancer Biology 2024Quote: ... platform and extraction kits (Promega, AS1390), following manufacturer’s instructions ...
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Tumor microenvironment acidosis favors pancreatic cancer stem cell properties and in vivo metastasisbioRxiv - Cancer Biology 2024Quote: ... and luciferase activity assessed by Dual Luciferase Reporter Assay (Promega, #E1910) in a Varioskan LUX microplate reader (Thermo Fischer Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... tumoral area was macrodissected prior DNA extraction using Maxwell 16 FFPE LEV DNA Purification Kit (Promega, Madison, WI, USA). DNA from the 60 non-CCHD carotid bodies were previously extracted and passed internal quality control at the VHIO’s laboratory before sequencing ...
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bioRxiv - Cancer Biology 2024Quote: ... RNase I (Promega) treatment was performed to cleave single-stranded RNA regions that were not hybridized with first-strand cDNA ...
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bioRxiv - Cancer Biology 2024Quote: ... their genomic DNA was extracted using the Wizard Genomic DNA Purification Kit (Promega, Madison, WI, USA), according to the manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: ... were supplemented with 1 µL of 1M ammonium bicarbonate (AmBic) and digested with 2 µL of 0.05 µg/µL sequencing grade trypsin (Promega) in 100 mM AmBic ...
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bioRxiv - Biochemistry 2024Quote: ... 1 µg of protein was diluted 5 times with 50 mM ABC and trypsin was added with a 1:20 trypsin to sample ratio (Promega, V5117). Protein were digested 24 h at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: ... 20 µg of protein was diluted 10 times with 50 mM ABC (no Guanidine) and trypsin was added with a 1:20 trypsin to sample ratio (Promega, V5117). Protein were digested overnight at 37 °C ...