Labshake search
Citations for Promega :
3001 - 3050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2024Quote: Escherichia coli HB101 (Promega, Madison, WI, USA) was used for plasmid construction and cultivated in LB medium (Bertani 1951 ...
-
bioRxiv - Biochemistry 2024Quote: ... Trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, WI, USA) was digested and incubated at 37°C for 16 h ...
-
bioRxiv - Biochemistry 2024Quote: ... and 100 μg of K562 cell tryptic digest (Promega) were added to 100 μL and 500 μL of 0.02% DMNG containing 0.1% TFA ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... Sequencing-grade modified trypsin (Promega) of 1:100 (w/w ...
-
bioRxiv - Genomics 2024Quote: ... DNA was extracted using Wizard® HMW DNA Extraction Kit (Promega) following the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2024Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
-
bioRxiv - Neuroscience 2024Quote: Split nanoluc fragments LgBit and SmBit (Promega) were cloned N-terminally to human CISD1 and point mutations introduced by site-directed mutagenesis using the Q5 Site-Directed Mutagenesis kit (NEB) ...
-
bioRxiv - Genetics 2024Quote: ... DNA was extracted using the Maxwell® RSC Blood DNA Kit (Promega) in accordance with the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: The IL2 URR was cloned into Xho I and Hind III sites of PGL4 Luc vector (Promega) and then ∼500 bp individual sequence representing IL2-IL21 intergenic CNS at −46 ...
-
bioRxiv - Molecular Biology 2024Quote: ... After AvrLm4-7 PCR amplification with external primers (Gautier et al., 2023) the PCR products were cloned into pGEM®-T Easy Vector System (PROMEGA) using standard protocols ...
-
bioRxiv - Systems Biology 2024Quote: ... cerevisiae protein lysate (V7461, Promega) to generate three samples ...
-
bioRxiv - Immunology 2024Quote: ... Reactions were performed with the GoTaq Probe 1-Step RT-qPCR System (Promega) following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cells were lysed in Reporter lysis buffer (Promega, Cat: E397A). The samples stored at -80°C were thawed and kept at RsT for 20 min ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... cell viability was assessed utilizing CellTiter-Glo® luminescence assay (Promega, #G755A) as per manufacturer’s instructions and measured with the SPARK 10M reader (Tecan) ...
-
bioRxiv - Pathology 2024Quote: ... primers are listed in Table S1) using the Green Master Mix (Promega) specific primers.
-
bioRxiv - Immunology 2024Quote: ... PCR was done on gDNA from at least 6 individual larvae using GoTaq Green Master Mix (Promega) (Supp Fig 1A ...
-
bioRxiv - Molecular Biology 2024Quote: ... Proteins were digested by adding 5 μL of sequencing grade trypsin (0.1 mg/mL) (Promega) for three h at 37 °C ...
-
bioRxiv - Biochemistry 2024Quote: ... Cells were then maintained for an additional 40 h and collected for Dual-Luciferase assays (Promega: E2920). In brief ...
-
bioRxiv - Genomics 2024Quote: ... and psPAX2 was used for packaging in 293T cells using FuGENE® HD Transfection Reagent (Promega, cat no. E2311). Lentivirus was concentrated using Lenti-X Concentrator (Takara Bio cat no ...
-
bioRxiv - Biochemistry 2024Quote: ... pH 8.0) containing 2 nM 11S and 1/250 Nano-Glo Luciferase Assay Reagent (Promega) was added to the cells and background luminescence read for 5 min ...
-
bioRxiv - Biochemistry 2024Quote: ... 100 μL of CellTiter-Glo 2.0 Reagent (Promega, G9241) was added to each well ...
-
bioRxiv - Biochemistry 2024Quote: ... Trypsin Gold (Promega, Fitchburg, WI, USA) was added at the ratio of 1:50 for enzyme digestion ...
-
bioRxiv - Biochemistry 2024Quote: Radioactively labelled proteins were synthesized in the presence of [35S]methionine in a standard or in a transcription and translation coupled reticulocyte lysate system (Promega). Mitochondria (final concentration 0.5 mg/ml ...
-
bioRxiv - Biochemistry 2024Quote: ... ffLuciferase activity in the lysate was determined by a luciferase assay (Promega, E1500) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2024Quote: ... passive lysis buffer (Promega, E194A) was added ...
-
bioRxiv - Biochemistry 2024Quote: ... ffLuciferase activity was determined from the lysate’s supernatant by a luciferase assay (Promega, E1500) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2024Quote: ... Protein was digested using sequencing-grade trypsin (Promega, V511) at 1:50 (20 µg trypsin:1 mg protein ...
-
bioRxiv - Biochemistry 2024Quote: ... Mass spectrometry-grade Trypsin/Lys-C mix (Promega) was used for digestion with an overnight incubation at 37°C ...
-
bioRxiv - Biochemistry 2024Quote: ... was incubated overnight at room temperature with 0.5 μg of sequencing-grade trypsin (Promega) in 0.1 M ammonium bicarbonate in a final volume of 20 μl ...
-
bioRxiv - Biochemistry 2024Quote: ... and 0.5% aprotinin] supplemented with 0.04 U/μL RNasin Plus RNase Inhibitor (Promega) and the cell suspension was passed 10 times through a 30-gauge needle and centrifuged ...
-
bioRxiv - Biochemistry 2024Quote: ... HaloTag-Oregon Green ligand (Promega), HaloTag-TMR ligand (Promega) ...
-
bioRxiv - Biochemistry 2024Quote: ... HaloTag succinimidyl ester (O2) ligand (Promega), HaloTag-Oregon Green ligand (Promega) ...
-
bioRxiv - Biochemistry 2024Quote: ... Trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, WI, USA) was digested and incubated at 37°C for 16 h ...
-
bioRxiv - Biochemistry 2024Quote: ... HaloTag-biotin ligand (Promega), horseradish peroxidase (HRP ...
-
bioRxiv - Biochemistry 2024Quote: ... Halo-γ-TuNA was then coupled to Halo Magne beads (Promega) and used to pull endogenous γ-TuRC out from X ...
-
bioRxiv - Biochemistry 2024Quote: ... incubated with ProTEV Plus protease (Promega), and dialyzed into Septin Storage Buffer (50 mM HEPES ...
-
bioRxiv - Biochemistry 2024Quote: ... ⑤ Add trypsin (Trypsin Gold, Promega, Fitchburg, WI, USA) for digestion according to trypsin:protein ratio of 1:50 and water bath at 37°C for 12-16 h ...
-
bioRxiv - Biochemistry 2024Quote: ... subtilis genomic DNA containing RsbT/RsbU was amplified by PCR using GoTaq DNA polymerase mix for thirty cycles without modification from the manufacturers protocol (Promega). DNA sequencing revealed that inserts had on average one to two mutations per product ...
-
bioRxiv - Biochemistry 2024Quote: ... then 20 µl Dual-Glo reagent (Promega) and the relative luminescence units were measured using an Envision microplate reader (PerkinElmer ...
-
bioRxiv - Biochemistry 2024Quote: ... The tryptic yeast proteome was obtained from Promega, which was reduced ...
-
bioRxiv - Biochemistry 2024Quote: ... with a mixture of trypsin and LysC (Promega) at an enzyme to substrate ratio of 1:50 ...
-
bioRxiv - Biochemistry 2024Quote: ... □ trypsin (Trypsin Gold, Promega, Trypchburg, WI, USA) was added at a ratio of 1:50 trypsin:protein for digestion and kept in a water bath overnight at 37 °C ...
-
bioRxiv - Biochemistry 2024Quote: ... Samples were digested at 47 °C for two hours with 600 ng of mass spectrometry grade Trypsin/LysC mix (Promega, V5113). The digested peptides were then eluted with two 35 µl increments of 0.2% formic acid in water and two more 40uL increments of 80% acetonitrile with 0.2% formic acid in water ...
-
bioRxiv - Neuroscience 2024Quote: ... cells were incubated with Fugene 6 (Promega) and plasmids for 12 hours ...
-
bioRxiv - Microbiology 2024Quote: Total RNA was extracted from WBC by using Eastep Super Total RNA Extraction Kit (Promega, China) according to the manufacturer’ s instruction ...
-
bioRxiv - Microbiology 2024Quote: Cell viability assays were performed with CellTiter-Glo 2.0 (Promega) according to the manufacturer’s instructions.
-
bioRxiv - Microbiology 2024Quote: ... the cells were infected with SFV-FFLuc (MOI=0.1) and lysed at 48 hpi using 1X Passive Lysis Buffer (PLB; Promega). FFLuc levels were measured using a Luciferase Assay System (Promega ...
-
bioRxiv - Microbiology 2024Quote: ... Relative luciferase intensities were determined using a Dual-luciferase reporter assay Kit (Promega), according to standard operating procedures.
-
bioRxiv - Synthetic Biology 2024Quote: ... This approach incorporated NanoLuc luciferase assays from Promega for the quantitative assessment of regulatory element functionality ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... 2 µL of DNA was amplified in a 25 µL mixture containing 12.5 µL of GoTaq® Green Master Mix 2X (Promega, Madison, Wisconsin, United States), 2.5 μL of each primer at 10 μM DFA (5’-GAYTTYGCNAGYYTNTAYCC-3’ ...