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Citations for Promega :
2451 - 2500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... PCRs were performed under standard conditions for High Performance GoTaq® G2 Flexi DNA Polymerase (Promega; M7801), with the addition of 5x combined enhancer solution [35] per reaction ...
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bioRxiv - Genomics 2024Quote: ... 1µl PGL3 Basic Vector (Promega, USA, Cat No: E1751) plasmid was added into all prepared standards ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR products were visualized on 1% agarose gels (90 V, 30-45 minutes) against a 1 kb DNA ladder (Promega). Sanger sequencing was performed by the sequencing unit at the Max Planck Institute for Evolutionary Biology ...
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bioRxiv - Genomics 2024Quote: ... The master mixtures consisted of 1 x Taq&Go G2 Hot Start colorless PCR Master Mix (Promega), 0.5 μM of each primer ...
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bioRxiv - Immunology 2024Quote: ... Anti-Rabbit IgG HRP (1:20,000 Promega W401B). Western blot quantification was done by using ImageStudioLite.
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bioRxiv - Genomics 2024Quote: ... Total genomic DNA was extracted from bacterial cultures using the Wizard® HMW DNA Extraction Kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... We used GoTaq G2 (Promega) with denaturation 94°C (2 min) ...
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bioRxiv - Evolutionary Biology 2024Quote: Total RNA was extracted from the whole body of each individual (male and female nymphs) using ReliaPrep RNA Tissue Miniprep Systems (Promega, Madison, WI, USA). The quality and quantity of the extracted RNA were evaluated using a NanoVue spectrophotometer (GE Healthcare Bio-Sciences ...
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bioRxiv - Genomics 2024Quote: ... [59] followed by a 1,653 nt firefly luciferase reporter sequence derived from plasmid pGL4.10 (Promega). Similarly ...
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bioRxiv - Cell Biology 2024Quote: ... and dsRNA was synthesised using the T7 RiboMAX express system (P1700, Promega). In a 6-well plate ...
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bioRxiv - Cell Biology 2024Quote: ... The monoclonal cells were screened for successful incorporation in the genomic locus of the fluorescent tag by PCR using GoTaq DNA Polymerase (Promega). The genome-edited cells were confirmed through imaging ...
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bioRxiv - Cell Biology 2024Quote: ... JF646-HaloTag ligand was purchased from Promega. JFX650-HaloTag ligand ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA was extracted using Eastep® Super Total RNA Extraction Kit (Promega). The PrimeScript RT Master Mix Kit (TaKaRa ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell growth was quantified using the Cell Titer-Glo Luminescent Cell Viability Assay (Cat# G7570, Promega) as described below.
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bioRxiv - Cancer Biology 2024Quote: ... Trypsin (Promega), Urea (Sigma) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell content was measured on Days 1-6 using Cell Titer-Glo Luminescent Cell Viability Assay (Cat# G7570, Promega). Luminescence was measured at an integration time of 0.5 second per well ...
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bioRxiv - Cancer Biology 2024Quote: ... or via CellTiter-Glo (Promega; G968A). Representative images were captured using an EVOS FL Auto Imaging System (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2024Quote: ... G17-1 cell proliferation was measured by MTS assay (Cell Titer 96 Aqueous One, Promega, Madison, WI, USA).
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bioRxiv - Cancer Biology 2024Quote: ... Protein digestion was carried out with sequencing grade modified Trypsin (Promega) at 1/50 protease/protein (wt/wt ...
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bioRxiv - Cancer Biology 2024Quote: Cell viability was measured by CellTiter-Glo bioluminescence (Promega, catalog no. G7572), and analyzed using an IVIS imager.
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was assessed 7 days post-treatment using the CellTiter-Glo® 3D Cell Viability assay (Promega).
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bioRxiv - Cancer Biology 2024Quote: ... and treated with 100 nM HaloTag NanoBRET 618 Ligand (Promega) or equivalent volume of DMSO as a no acceptor negative control ...
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bioRxiv - Cancer Biology 2024Quote: ... cell viability was measured by addition of resazurin according to manufacturer’s instructions (Promega).
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bioRxiv - Cancer Biology 2024Quote: NanoBRET assays were performed as described by Promega. HEK293T cells were cultured as described above and transfected with a 1:10 ratio of Nluc-ERa:14-3-3σ-HaloTag plasmid for 48 hours using jetOPTIMUS transfection reagent (Polyplus) ...
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bioRxiv - Cancer Biology 2024Quote: ... after which the ERα activity was determined with a Dual-Luciferase Reporter Assay (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... pH 8.0 were added and proteins were digested with 1.5 μl trypsin (0.5 μg/μl; Promega) with constant shaking at 1,100 rpm and incubation at 37 °C on a ThermoMixer C incubator for 4 h ...
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bioRxiv - Physiology 2024Quote: ... further steps were performed according to the manufacturer’s protocol using proteases trypsin and Lys-C (2 h at 47° C, 1:15 and 1:30, respectively, Promega, USA). Peptides (20 μg ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The luciferase expression vector pGL4.10 was purchased from Promega (Madison, WI). Lentivirus vectors expressing shRNAs targeting CEBPB were obtained from the University of Minnesota Genomics Center (Sh1 ...
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bioRxiv - Neuroscience 2024Quote: ... The PCR fragment was subcloned into the pGEM-T vector (A1360, Promega) to generate pLM1 (for details ...
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bioRxiv - Cancer Biology 2024Quote: ... then 25 uL of CellTiter-Glo (Promega) was added to each well ...
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bioRxiv - Cancer Biology 2024Quote: ... The CellTiter-GLO kit from Promega (G7570) was utilized ...
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bioRxiv - Cancer Biology 2024Quote: 1,000 HCECs or M158 cells were plated per well of 96-well plates and proliferation was measured over time using CellTiter Blue (Promega) and a plate reader ...
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bioRxiv - Cell Biology 2024Quote: ... or HaloTag (Promega) were enriched by fluorescence- activated cell sorting (FACS ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was determined using CellTiterGlo reagent (Promega) following the manufacturer’s recommended protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... was used to determine cell viability using a Glomax Multi Detection System (Promega).
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bioRxiv - Cancer Biology 2024Quote: ... were transfected with GFP-H3.3 or GFP-H3K9M plasmids using FuGENE HD Transfection Reagent (Promega, cat# E2311). After 48 hrs ...
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bioRxiv - Cancer Biology 2024Quote: ... (Promega Cat#G9241). 100 ml of cell titer-glo was added to each well and incubated shaking in the dark at room temperature for 10 minutes before reading luminescence using a plate reader.
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bioRxiv - Cell Biology 2024Quote: ... ApoLive-Glo Multiplex Assay Kit (Promega, Madison ...
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bioRxiv - Cell Biology 2024Quote: ... 3 µl of nuclease free water (Promega, Cat No. P119E), and 5 µl of cDNA using the following program ...
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bioRxiv - Cell Biology 2024Quote: ... RT-qPCR analysis was done on a QuantStudio™ 5 Real-Time PCR System in a final volume of 20 µl containing 10 µl of GoTaq qPCR Master Mix (Promega, Cat No. PRA6001), 2 µl of the forward plus reverse primers (final primer concentration ...
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bioRxiv - Cell Biology 2024Quote: ... following manufacturer’s instructions using random primers (Promega). RT-qPCRs were carried out on a Quantstudio 3 Applied Biosystems real-time PCR system (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... 0.05 nM JF549 (GA1110; Promega) dye was mixed with 10 nM JF646 dye (GA1120 ...
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bioRxiv - Cancer Biology 2024Quote: ... or luminescence measurement using CellTiter-Glo (Promega; G968A).
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bioRxiv - Molecular Biology 2024Quote: ... coli BL21(DE3) pLysS cells (Promega). Cells were grown in Lysogeny broth to an OD 600 of 0.5-0.7 and protein expression was induced with 0.5 mM IPTG for 20 h at 16 °C ...
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bioRxiv - Neuroscience 2024Quote: ... the 130-kDa band was cut out as a gel piece and digested with trypsin (Promega) at 37°C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... 50 μg of protein per sample was digested with trypsin (Promega) overnight at 37°C using S-TRAP micro cartridges (Protifi LLC,) ...
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bioRxiv - Molecular Biology 2024Quote: ... and circRNAs were semi-quantitatively amplified using GoTaq® DNA Polymerase (Promega, M3005); 4µl of 1:2.5 diluted cDNA ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... Secondary antibodies were diluted in blocking buffer and incubated with the membrane for 60 min at room temperature (1:10,000 anti-rabbit-HRP, Promega #W401B). After secondary antibody incubation ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by in-gel digestion with trypsin (Promega) at 37°C for overnight ...
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bioRxiv - Microbiology 2024Quote: ... or the Magne-His purification system (Promega Corporation) for PlzARD-RD per manufacturer protocol ...