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Citations for Promega :
2301 - 2350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... and cloned with the pGEM®-T Vector Systems (Promega). For each band ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and viability was measured using RealTime Glo (#G9711, Promega) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Cloning of the different PCR products was performed as described in the pGEM®-T Vector system according to the instructoŕs instructions (Promega). Sequencing was performed at LGC Genomics (Berlin ...
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bioRxiv - Neuroscience 2024Quote: ... GoTaq G2 Flexi DNA Polymerase enzyme (Promega, M7806) was used to amplify Daam1-MIC’s flanking exons and PCR assays were performed with 25 cycles ...
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bioRxiv - Plant Biology 2024Quote: ... 2 µL ROX (CRX reference dye, Promega, C5411) were added to 1 mL SybrGreen as a passive reference dye that allows fluorescent normalization for qPCR data ...
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bioRxiv - Plant Biology 2024Quote: ... Products were cleaned with the Wizard® SV Gel and PCR Clean-Up System (Promega) and cloned with the pGEM®-T Vector Systems (Promega) ...
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bioRxiv - Plant Biology 2024Quote: Stem-loop PCR and 5’-RLM-RACE PCR amplification products were purified using The Wizard® SV Gel and PCR Clean-Up System (Promega). Cloning of the different PCR products was performed as described in the pGEM®-T Vector system according to the instructoŕs instructions (Promega) ...
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bioRxiv - Evolutionary Biology 2024Quote: The Dual luciferase reporter assay kit (Promega, USA) was used to measure the reporter gene expression ...
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bioRxiv - Evolutionary Biology 2024Quote: ... this regulatory sequence was constructed into a pGL3-promoter vector (Promega), which contains the SV40 enhancer and firefly luciferase ...
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bioRxiv - Synthetic Biology 2024Quote: ... and activity was measured with Luciferase Assay System (Promega, Madison, WI) using vendor-suggested procedures ...
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bioRxiv - Microbiology 2024Quote: ... DNA bands at ∼390bp were excised from gel and purified using Wizard™ SV Gel and PCR Cleanup System (Promega A9282) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... BrdU incorporation was detected by measuring absorbance at 450 nm using a GloMax plate reader (Promega).
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bioRxiv - Genomics 2024Quote: ... RNA was extracted with the Maxwell RSC Plant RNA kit (Promega, Madison, WI, USA) using the Maxwell® RSC device according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... The ground material was used for genomic DNA extraction with the RSC Plant DNA Kit (Promega, Madison, WI, USA) using the Maxwell® RSC device according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: Total RNA was purified from 10-15 midguts using the ReliaPrep RNA Tissue Miniprep System (Promega). cDNA was made from 100 or 200 ng of RNA using PrimeScript RT Reagent Kit (TaKaRa) ...
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bioRxiv - Immunology 2024Quote: ... LDH activity was quantified in all cell supernatants using the Cytox96 non-radioactive cytotoxicity assay (Promega). Absorbances at 492 and 620 nm were measured with a Tecan Microplate Reader and reported as percentages of the value measured in the supernatant of cells treated with 1% triton for 10 minutes (total LDH).
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bioRxiv - Microbiology 2024Quote: ... Lanes containing proteins were excised in two bands and subjected to a manual in-gel digestion with modified porcine trypsin (Trypsin Gold, Promega). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... the BacTiter-Glo microbial cell viability assay kit (Promega, USA) was employed for this purpose ...
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bioRxiv - Microbiology 2024Quote: ... 20 μM of EnduRen live-cell substrate (Promega, E6481) was added to the cells in DMEM and incubated for at least 1 hour before detection using the Varioskan LUX Multi-well Luminometer (Thermo Fisher Scientific).
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bioRxiv - Immunology 2024Quote: ... The cDNA was synthesized from 2μl of the eluted RNA using M-MLV reverse transcriptase (Promega) and random hexamer primers ...
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bioRxiv - Microbiology 2024Quote: ... cloned into pGEM-3Z (Promega) using In-Fusion technology (Takara ...
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LRBA balances antigen presentation and T-cell responses via autophagy by binding to PIK3R4 and FYCO1bioRxiv - Immunology 2024Quote: Human LRBA was cloned in seven different fragments into pCIneo FLAG vectors (Promega). These plasmids were kindly provided by Dr ...
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bioRxiv - Immunology 2024Quote: ... an equal volume of One-Glo reagent (Promega, E6120) was added to the culture medium for readout ...
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bioRxiv - Microbiology 2024Quote: Plasmids used as standard to normalize the qPCR data were obtained by blunt end ligation of the flavivirus qPCR products into pGEM-3Z (Promega), using SmaI (NEB ...
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bioRxiv - Microbiology 2024Quote: ... and detected with a GloMax 20/20 Luminometer (Promega) or Varioskan LUX Multi-well Luminometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Intracellular luciferase activity (Relative light units, RLU) was measured using the Bright-Glo Luciferase Assay Kit (Promega, E2620) and detected with a GloMax 20/20 Luminometer (Promega ...
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bioRxiv - Microbiology 2024Quote: ... and PCR (Promega #M7806) using primers flanking the sequence targeted by the sgRNA (Table S2 ...
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bioRxiv - Microbiology 2024Quote: ... reverse transcription reactions were performed by adding 0.5 µl (corresponding to 5 U) of AMV Reverse Transcriptase (Promega), 0.1 µl dNTP mix (10 mM) ...
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bioRxiv - Immunology 2024Quote: Cytokine or chemical inhibitor doses were selected based on available EC50 data and toxicity profiles with untreated macrophages (Trypan Blue staining or MTS assay; CellTiter 96 Aqueous One Solution Cell Proliferation Assay Promega, Madison, WI, USA). Only inhibitor doses resulting in >90% cell viability were utilized ...
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bioRxiv - Immunology 2024Quote: ... Triglyceride-Glo™ Assay and Cholesterol/Cholesterol Ester-Glo™ Assay kits (Promega; Madison, WI, USA) were used to quantify triglycerides and cholesterol ...
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bioRxiv - Genomics 2024Quote: ... The plate was then incubated at room temperature for 10 min to stabilize the luminescent signal and recordings were taken on a GloMax Discover System (Promega).
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bioRxiv - Genomics 2024Quote: ... The column was washed twice with 150 µL of loading buffer and gently centrifuged before overnight digestion at 37°C with 0.1 µg/µL of trypsin (Promega) in 50 mM ammonium bicarbonate ...
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bioRxiv - Genetics 2024Quote: ... YFP-negative first instar larvae of the progeny of gwnull- 1/PBac(681.P.FSVS-1)PMCACPTI001995 or gwnul1-2/PBac(681.P.FSVS-1)PMCACPTI001995 were collected 38 hr after egg laying and were lysed in Passive lysis buffer (Promega). After centrifugation ...
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bioRxiv - Plant Biology 2024Quote: ... and size-selected using the ProNex Size-Selective Purification System (Promega). cDNA libraries were then PCR-amplified with 13 cycles and the final library quality was assessed using a TapeStation System (Agilent Technologies) ...
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bioRxiv - Biochemistry 2024Quote: ... Luciferase activity assays were performed using Dual-Luciferase Reporter Assay System (Promega, Cat#E1910) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... 2 U RNase-free DNase (Promega), 1 mM CaCl2 ...
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bioRxiv - Biophysics 2024Quote: ... we used the luciferase assay system from Promega (Promega, Cat. No.: E1501). To do so ...
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bioRxiv - Biochemistry 2024Quote: ... MCF10A cells were grown in a 10 cm dish until they reached 70-80% confluency and then were transfected with CBF:H2B-Venus using manufacturer’s protocol (FuGene HD, Promega, E2311). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR fragment was directionally cloned downstream of the Renilla luciferase ORF in the psiCHECK-2 vector (Promega, Madison, US) using the Quick Ligase kit (M2200 ...
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bioRxiv - Molecular Biology 2024Quote: ... Renilla and Firefly luciferase signals were measured using the Dual-Glo® luciferase reporter gene assay (Promega, Madison, US). Each experiment was performed three times.
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bioRxiv - Molecular Biology 2024Quote: ... Reporter activity was evaluated 48 hours after transfection using a dual-luciferase assay according to the manufacturer’s protocol (Promega). Firefly luciferase measurements were normalized to the phRL-TK activity ...
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bioRxiv - Biophysics 2024Quote: ... we used the luciferase assay system from Promega (Promega, Cat. No.: E1501). To do so ...
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bioRxiv - Biophysics 2024Quote: ... we stained the cells with 1 ml of a 1:2,000 TMR Halo-ligand solution (Promega, Cat. No.: G8251) to fluorescently label the Halo-tagged proteins ...
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bioRxiv - Biophysics 2024Quote: ... prior to incubation with the primary anti-Halo-Tag antibody (Mouse monoclonal antibody, Promega, Cat. No.: G9211). For incubation with the primary anti-RBPJ antibody (Rat monoclonal antibody ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA samples were subjected to reverse-transcription (RT) using the Go Script reagent (Promega) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The estimation of protein concentration was done using BCA protein assay (Promega #PI-23222, PI-23224). Samples were diluted using 1 X sample buffer (4 X stock ...
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bioRxiv - Cancer Biology 2024Quote: ... or combination using the CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega). Plates were read using a CLARIOStar microplate reader (BMG Labtech).
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were digested with sequencing-grade modified trypsin (Promega, Madison, WI) overnight at 37 °C ...
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bioRxiv - Bioengineering 2024Quote: ... Using the GloMax 96 Microplate Luminometer (Promega), 25 μl NanoGlo reagent were added to each well by automatic injection and immediately analyzed.
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bioRxiv - Biochemistry 2024Quote: ... they were transfected with indicated plasmids using FuGENE 6 transfection reagent (Promega). After another 24h ...