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Citations for Promega :
2251 - 2300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 0.5 μg/μl stock of trypsin (Promega, V5111) dissolved in 40 mM acetic acid was added to each sample for an enzyme:protein ratio of 1:100 ...
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bioRxiv - Microbiology 2024Quote: ... Contaminating DNA in the samples were removed through incubation at 37°C for 2 h using RNase-free DNase I (Promega, USA). All RNAs in the samples were converted into cDNA using a cDNA EcoDry Premix (Clontech ...
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bioRxiv - Microbiology 2024Quote: ... Luciferase assays were performed by Dual-Glo® Luciferase Assay System (Promega, Cat#: E2920). Firefly and Renilla luciferase activities were measured by Synergy2 (BioTek ...
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bioRxiv - Microbiology 2024Quote: ... and the Renilla luciferase control vector (Promega, Madison, WI) using Lipo8000™ transfection reagent (Beyotime ...
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bioRxiv - Plant Biology 2024Quote: ... using trypsin (10 ng/μl, V5113, Promega) to digest the phosphorylated OsPrx20-His and analyzed by the LC-MS/MS system (LTQ Orbitrap XL ETD ...
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bioRxiv - Plant Biology 2024Quote: ... the sample was digested with 400 ng trypsin (Trypsin Gold, Promega) at 37°C overnight ...
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bioRxiv - Genetics 2024Quote: ... dNTP mix (2.5mM each, Promega; U1515), ERCC RNA Spike-In Mix (1:3.2x107 Thermo Fischer Scientific 4456740 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 250 μM of each dNTP (Promega, Cat # R0192), 1mg/mL of bovine serum albumin (BSA ...
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bioRxiv - Immunology 2024Quote: ... plates were washed 5 times with wash buffer (PBS with 1% BSA (Capricorn Scientific) and 0.05% Tween-20 (Promega)) ...
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bioRxiv - Evolutionary Biology 2024Quote: DNA was extracted from the whole body of dissected beetles using the Wizard Genomic DNA Purification Kit (Promega). The concentration of extracted DNA was estimated using a Qubit fluorimeter (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... 100 U/mL RNasin® Ribonuclease Inhibitor (Promega)) ...
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bioRxiv - Microbiology 2024Quote: ... These fragments were cloned into expression plasmids based on a pCI-neo mammalian expression vector (Promega, Madison, WI, U.S.A.), e.g ...
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bioRxiv - Microbiology 2024Quote: ... Quantitative PCR was performed using the GoTaq qPCR SYBR mastermix (Promega) on a LightCycler 480 instrument (Roche) ...
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bioRxiv - Immunology 2024Quote: ... FuGENE® was from Promega, while cholera toxin B (CTB ...
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bioRxiv - Molecular Biology 2024Quote: ... and corresponding controls were reverse transfected in HepG2 cells in triplicates as described above and a glucose uptake assay (Glucose Uptake-GloTM Assay, Promega, Madison, US) was performed ...
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Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: HEK293 cells growing at 70% confluency in a 10-cm dish were transfected with wild-type human NOPR or NOPLight (3 μg DNA) and GloSensor-20F (Promega, 2.5 μg DNA) using 12 μL Lipofectamine 2000 (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 µL of CellTiter-Glo reagent (Promega) were added to each well ...
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bioRxiv - Cell Biology 2024Quote: ... 30 μL of FuGENE HD (Promega) was added for every mL of DNA solution ...
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bioRxiv - Cell Biology 2024Quote: ... 50 μL aliquots were taken and measured using the nano-glo luciferase assay system according to the manufactures protocol (Promega). For differential enrichment ...
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bioRxiv - Cell Biology 2024Quote: ... using FuGENE HD transfection reagent (Promega) as instructed by manufacturer ...
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bioRxiv - Cancer Biology 2024Quote: ... Luminescence was recorded using a GloMax® luminometer (Promega).
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bioRxiv - Cancer Biology 2024Quote: ... with 250 cells plated per well in 96-well flat-bottom plates in 100 ml medium and an equal volume of the CellTiter-Glo Luminescent Assay Reagent (#G7571; Promega) added at the indicated time-points ...
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bioRxiv - Cancer Biology 2024Quote: ... RS4;11 cells were transduced to express the luminescent reporter luciferase using pGL4.51[luc2/CMV/Neo] vector (Promega). Cell authentication was performed using short tandem repeat analysis (Idexx BioAnalytics ...
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bioRxiv - Cancer Biology 2024Quote: ... colony numbers were assessed using CellTiter-Glo 2.0 reagent (Promega #G9242) and luminescence was measured (POLARstar Optima plate reader ...
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bioRxiv - Cancer Biology 2024Quote: ... and wild-type Melan-a cells were transfected using Fugene transfection reagent (Promega Catalog # E5911). As a result ...
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bioRxiv - Bioengineering 2024Quote: ... Trypsin (Promega) was added at a 1:20 w:w ratio and digestions proceeded at room temperature overnight ...
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bioRxiv - Bioengineering 2024Quote: The primary antibodies used in this study were the following: rb-α-Nanoluc (non-commercial, Promega, 1:1000); rb-α-CD63 (ab68418 ...
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bioRxiv - Bioengineering 2024Quote: ... NanoGlo reagent (NanoGlo Luciferase Assay System, Promega) was prepared from NanoGlo lysis buffer and NanoGlo substrate solution according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were digested using 0.005 µg/µl trypsin (sequencing grade modified trypsin; Promega) in 40 mM NH4HCO3/10% ACN at 4°C for 20 minutes followed by 16 h at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... and 40U/ml RNAsin plus (Promega). Lysates were sonicated using a Bioruptor (Diagenode ...
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bioRxiv - Cancer Biology 2024Quote: ... (G5421, Promega, Belgium), and CellTiter® 3D Cell Viability Assay (G9682 ...
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bioRxiv - Biochemistry 2024Quote: ... reduced and digested with trypsin (mass spectrometry grade; Promega Corporation) at a trypsin:protein ratio of 1:20 and peptides were purified using C18 tips (Pierce C18 Tips ...
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bioRxiv - Microbiology 2024Quote: ... Peptide digestion was initiated by adding 25 µL of 50 mM ammonium bicarbonate buffer (pH 8) containing 2 µg trypsin (Sequencing Grade Modified Trypsin, Promega, Cat. no. V5117) directly on top of the column and incubating overnight at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... Cell viability was assessed using CellTiter-Glo® Luminescent Cell Viability Assay (Promega, Cat. no. G7570) as per manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... Fugene HD transfection reagent (Promega; catalogue number: E231A) was used for transient transfections ...
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bioRxiv - Cancer Biology 2024Quote: ... Afer 24 h levels of firefly luciferase and renilla luciferase in each well were measured using the DualGlo Luciferase Assay System (Promega), and the ratio of firefly luciferase to renilla luciferase was calculated.
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bioRxiv - Cancer Biology 2024Quote: ... Samples were then diluted to 1 M urea and subjected to digestion with trypsin (Promega, 1:50 w/w) overnight at 37⁰C ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 ng of reporter vector plus 50 ng of the renilla luciferase plasmid was transfected to cells using FuGENE 6 (Promega); cells were then treated with DMSO ...
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bioRxiv - Cancer Biology 2024Quote: ... Reporter assays were performed using a dual luciferase reporter assay system (Promega) by Fluoroskan Ascent FL fluorometer (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2024Quote: ... after which 20 µL 1 mg/mL trypsin (Promega, Madison, WI) was added to each sample ...
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bioRxiv - Cell Biology 2024Quote: ... alkylated with 2-iodoacetamide and digested with Endopeptidase Trypsin (sequencing grade, Promega) overnight ...
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bioRxiv - Cell Biology 2024Quote: pabp2 and gfp (used as control) dsRNA was prepared from gel eluted (Promega, A9282) PCR product using T7 RNA polymerase (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then treated with or without 100 µM dNTPs mix (Promega) and were harvested 0 ...
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bioRxiv - Cell Biology 2024Quote: ... qPCR was performed using the Gotaq qPCR Master Mix (Promega) with the StepOne Real Time PCR (Applied Biosystems ...
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bioRxiv - Microbiology 2024Quote: RNA was incubated at 95°C for 2 min and on ice for 2 min before 3.3 x RNA folding buffer (100 mM HEPES pH 8.0, 100 mM NaCl and 10 mM MgCl2) and RNasin Plus RNase Inhibitor (Promega) was added to a final volume of 10 μl and incubated for 20 min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... monolayers were lysed in IP lysis buffer (Promega) and incubated at room temperature for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... followed by digestion with Endoproteinase LysC (Wako Chemicals) and trypsin (Promega). Reaction was halted by addition of trifluoracetic acid and peptides were solid phase extracted (46 ...
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bioRxiv - Plant Biology 2024Quote: ... DNA was dissolved in milliQ water and treated with RNase (Promega) for 30 minutes at 37 ◦C.
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bioRxiv - Molecular Biology 2024Quote: ... ATRX 5ʹ-TGAAACTTCATTTTCAACCAAATGCTC-3ʹ and 5ʹ-ATCAAGGGGATGGCAGCAG-3ʹ All PCR reactions were performed using GoTaq® G2 DNA polymerase kit from Promega following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... Trypsin (Promega, Madison, WI, USA) and Lys-C (Wako ...