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Citations for Promega :
2551 - 2600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... both enzymes were purchased from Promega. The reactions were stopped by adding 1% trifluoroacetic acid (TFA ...
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bioRxiv - Biochemistry 2024Quote: ... The firefly and renilla luminescence were quantified using a Dual-Glo® Luciferase Assay System (Promega, USA) and the firefly/renilla luminescence ratios calculated.
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bioRxiv - Biochemistry 2024Quote: ... Trypsin was obtained from Promega (V511 sequencing grade). LysC was obtained from Wako (125-05061 sequencing grade) ...
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bioRxiv - Biochemistry 2024Quote: HEK293T cells seeded in 12-wells plates coated with poly-D-lysine were transfected with 500 ng of empty vector pHTN HaloTag CMV-neo (Promega G7721) or pCEFL-HaloTag-P-Rex1 constructs and co-transfected with 500 ng of SRE-firefly luciferase and 50 ng of Renilla luciferase plasmids ...
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bioRxiv - Developmental Biology 2024Quote: ... The luminescent signal was measured after 45 min using GloMax® Discover Microplate Reader (Promega, Madison, USA), kindly provided by the Department of Zoology (190z) ...
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bioRxiv - Molecular Biology 2024Quote: ... Six washes were performed before adding the trypsin (Promega) at 1/20 ratio and incubation for 2 h at 47°C ...
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bioRxiv - Plant Biology 2024Quote: ... Transfected cells were cultured for 16-18 h followed by analysis of relative luciferase (LUC) activity using the Dual-Luciferase Reporter Assay System (Promega), which detects the activities of firefly LUC and the internal control Renilla reniformis (REN) ...
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bioRxiv - Bioengineering 2024Quote: ... and at 580-640nm (Glomax, Promega).
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bioRxiv - Bioengineering 2024Quote: ... and 1000 U/mL RNase inhibitor (Promega, N2615), 0.001 M DTT ...
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bioRxiv - Molecular Biology 2024Quote: ... 100U/mL RNase inhibitor (Promega) and EDTA-free proteinase inhibitor (Roche)) ...
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bioRxiv - Biochemistry 2024Quote: ... using 100 mM ammonium bicarbonate (pH 8.0) and 0.5 μg of Trypsin gold (Promega). Digested fractions were split 50/50 ...
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bioRxiv - Cancer Biology 2024Quote: ... The dye was released from cell inserts using 30% acetic acid and absorbance was measured using GloMax Explorer (Promega) microplate reader at 590 nm as a readout for cell migration.
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bioRxiv - Biophysics 2024Quote: ... cells were transiently transfected with 2 µg DNA by lipofection (FuGENEⓇ HD, Promega, Madison, WI).
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bioRxiv - Cancer Biology 2024Quote: ... and concentration was measured using the Quantus™ Fluorometer (Promega, E6150) with QuantiFluor® ONE dsDNA dye (Promega ...
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bioRxiv - Cancer Biology 2024Quote: ... with QuantiFluor® ONE dsDNA dye (Promega, E4870). Rotating incubation was done using a Tube Revolver Rotator from Thermo Scientific™ (88881002).
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bioRxiv - Physiology 2024Quote: ... then it was reverse transcribed into cDNA using AMV reverse transcriptase (Promega). Finally ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA concentration was measured using a Quantus™ Fluorometer (Promega, E6150) with QuantiFluor® ONE dsDNA dye (Promega ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were digested in-gel overnight at 37°C with Sequencing Grade Modified Trypsin (Promega; cat. #V5111) diluted in 200uL of digestion buffer (40 mM ammonium bicarbonate ...
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bioRxiv - Cell Biology 2024Quote: ... cells were gently washed once with 1X DPBS and MTS reagent of 20 μL (Cell Titer-96 Aqueous One Solution, Promega, USA) in 80 μL of cell culture media for 3 h (37°C and 5% CO2) ...
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bioRxiv - Biochemistry 2024Quote: Kinase assays were performed using the ADP-Glo Kinase Assay kit (Promega) as follows ...
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bioRxiv - Biochemistry 2024Quote: ... was then incubated with nucleosome substrate (300 nM) for 30 minutes at room temperature following addition of 500 µM ATP (Promega) in a reaction volume of 20 µL (in 50 mM Tris-Cl pH 7.6 ...
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bioRxiv - Biochemistry 2024Quote: ... RNasin Plus RNase inhibitor (Promega), and nuclease-free water (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... NanoBRET NanoGlo detection system (Promega, N1662) and NanoGlo Live cell assay system (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... and NanoGlo Live cell assay system (Promega, N2012) were used for NanoBRET and split Nanoluciferase complementation Systematic Protein Affinity Strength Modulation (SPASM ...
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bioRxiv - Biochemistry 2024Quote: ... The translation mixtures were incubated at 30°C for 90 mins followed by the dual-luciferase reporter assay (Promega). The 2μl translation products were added into a white 384-well microplate (PerkinElmer) ...
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bioRxiv - Biochemistry 2024Quote: Cell-free translation (CFT) was performed using wheat germ extract (Promega) supplemented with Complete Amino Acid Mixture (Promega) ...
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bioRxiv - Biochemistry 2024Quote: ... coli KRX cells (Promega) in phosphate-buffered Terrific Broth (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... supplemented with Complete Amino Acid Mixture (Promega), potassium acetate (Promega) ...
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bioRxiv - Biochemistry 2024Quote: ... Nano-Glo HiBiT lytic detection system (Promega) was diluted as per the manufacturers’ instructions and 5 µl were added to each well by multichannel pipette ...
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bioRxiv - Biochemistry 2024Quote: ... Oligo(dT) Protocol (Promega, A2791). RT-qPCR was set up in Thermo Fisher Scientific QuantStudio™ 5 Real-Time PCR System and the results were analyzed using Design and Analysis software 2.6.0 ...
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bioRxiv - Biochemistry 2024Quote: ... CellTiter-Glo reagent (cat#G7572) was obtained from Promega GmbH (Walldorf ...
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bioRxiv - Biochemistry 2024Quote: ... and then digested overnight at 37 °C and shaking at 1000 rpm (TC) with trypsin (Sequencing Grade Modified Trypsin V5113, Promega) and LysC (Lysyl Endopeptidase 129-02541 ...
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bioRxiv - Biochemistry 2024Quote: ... fluorofurimazine was obtained from Promega as a ready-to-use poloxamer-407 (P-407 ...
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bioRxiv - Biochemistry 2024Quote: ... Furimazine was purchased as part of the Nano-Glo Assay kit (Promega), fluorofurimazine was obtained from Promega as a ready-to-use poloxamer-407 (P-407 ...
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bioRxiv - Biochemistry 2024Quote: ... we measured luciferase activity using the Dual-Glo Luciferase Assay Kit (Promega). All measurements were performed in technical triplicate ...
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bioRxiv - Biochemistry 2024Quote: ... nanoLuc signal was assessed using the Nano-Glo Luciferase Assay System (Promega) according to the manufacturer’s instructions and the signal was quantified on a Hidex Sense plate reader with 1-second reads and an open filter setting ...
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bioRxiv - Plant Biology 2024Quote: ... according to the manufacturer’s instructions and treated with RQ1 RNase-free DNase (Promega). cDNA was synthesized with ImProm-II™ Reverse Transcription System (Promega) ...
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bioRxiv - Cell Biology 2024Quote: ... Apoptosis was measured using RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay kit (Promega) following manufacturer’s instructions.
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bioRxiv - Biochemistry 2024Quote: ... and 2 µL of 500 ng/µL Trypsin Platinum (Promega, Madison, WI, USA). Thereafter ...
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bioRxiv - Genomics 2024Quote: ... (34) DNA template and unused depletion probes were removed through addition of 4 μL RNase-free DNAse (Promega) with 5 μL 10 mM CaCl2 in 30 μL Nuclease-free water and incubated at 37 °C for 30 min ...
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bioRxiv - Systems Biology 2024Quote: ... Trypsin Gold (V5280, Promega) was added to a final concentration of 20 ng/µL ...
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bioRxiv - Genomics 2024Quote: The promoter of bovine RHO was cloned into a pGL3 luciferase reporter vector (Promega E1741). HEK293 cells were transfected with plasmids expressing the RHO reporter vector ...
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bioRxiv - Genomics 2024Quote: ... Luciferase activity was performed 48 hrs following ASO transfection with the Firefly Luciferase assay system (Promega E4030), following the manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... 50 µL of Cell Titer Glo (Promega) was added to each well in the 96 well plate ...
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bioRxiv - Cancer Biology 2024Quote: ... Trypsin (Promega MS grade) was then added at a ratio of 1:50 to the total protein mass in the sample ...
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bioRxiv - Cancer Biology 2024Quote: ... and digested overnight at room temperature with 1 µg/µL endoproteinase Lys-C (Wako) followed by a digestion with sequencing-grade trypsin (Promega) at a final concentration of 1 ng/μL 6 h at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... The proteins were then digested by trypsin (sequencing-grade, Promega) at 1:50 ratio at 37 °C overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... Medium was aspirated and discarded before 30 µl undiluted CellTiter-Glo (Promega) solution was added to each well ...
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Tumor microenvironment acidosis favors pancreatic cancer stem cell properties and in vivo metastasisbioRxiv - Cancer Biology 2024Quote: ... and 0.3 µg pRL-TK (Promega) employing Lipofectamine 3000 (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... Trypsin (Promega) was added to a 1/100 trypsin to protein ratio and the samples were incubated overnight at 37°C ...