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Citations for Promega :
2351 - 2400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... The following viability assay kits were used: CellTiter-Glo® Luminescent Cell Viability assay (G7571, Promega, Belgium), CellTiter 96® Aqueous Non-Radioactive Cell Proliferation Assay ...
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bioRxiv - Cancer Biology 2024Quote: ... and CellTiter® 3D Cell Viability Assay (G9682, Promega, Belgium). Normalization of the ATP assays was done by Pierce BCA Protein assay kit (23227 ...
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bioRxiv - Bioengineering 2024Quote: ... the effluent was collected and analyzed for lactate and glutamate production using the Lactate-GloTM (Promega, #J5021) and Glutamate-GloTM (Promega ...
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bioRxiv - Cancer Biology 2024Quote: ... using an automated RNA/DNA isolation instrument (Maxwell, Promega). cDNAs were synthesized using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted using the Maxwell® RSC simplyRNA Cells and RNase-free DNase Kit (Promega; AS1390) using an automated RNA/DNA isolation instrument (Maxwell ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was assessed by using CellTiter-Glo 2.0 reagent (G9242, Promega). After 48-72 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... Renilla-Glo Luciferase assay (Promega) was performed as per manufacture instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNAs were isolated using Maxwell® RSC Cultured Cells DNA Kit (Promega; AS1620) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Cell Titer Glo 2.0 Cell Viability Assay (Promega) was performed on primary cortical neurons following manufacturer instructions including a standard curve from 0.1 nM to 100 μM ATP in each plate for standardization ...
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bioRxiv - Neuroscience 2024Quote: ... 1.5 µg of total RNA was converted into single-stranded cDNA using the ImPromII reverse transcriptase kit (Promega) with random primers (0.5 µg/ml) ...
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bioRxiv - Neuroscience 2024Quote: ... in addition to the transgene of interest using FuGene HD (Promega). Media was harvested at 48- and 72-hours post-transfection ...
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bioRxiv - Neuroscience 2024Quote: ... 10μg/ml AcBSA (Promega), 100mM KCl in 1X PBS ...
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bioRxiv - Neuroscience 2024Quote: ... For experiments cells were plated onto poly-D-lysine coated glass coverslips supplemented with 1 μM all-trans retinal and transfected using FuGENE® HD (Promega, Madison, USA) the next day ...
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bioRxiv - Neuroscience 2024Quote: ... Reverse transcription was performed with 1 μg RNA using reverse transcriptase (Promega) and random hexamers for cDNA synthesis ...
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bioRxiv - Physiology 2024Quote: ... Protein digestion was performed with Lysyl Endopeptidase (enzyme:substrate 1:100, 125-05061, Wako Pure Chemical Industries) and trypsin (enzyme:substrate 1:50, Promega V5113) overnight at 37°C with agitation (1,000 rpm) ...
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bioRxiv - Plant Biology 2024Quote: RNA was extracted with the SV Total RNA Isolation System (Promega) then RNA was treated with DNase I (RNase-free ...
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bioRxiv - Plant Biology 2024Quote: ... After the DNAse treatment with RQ1 DNAse (Promega, USA), 1 µg or total RNA was used for the cDNA synthesis (GoScript ™ Reverse Transcription System ...
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bioRxiv - Plant Biology 2024Quote: ... 25 µg of phloem sap and 50 µg of leaf tissue protein extract were then digested in solution using a Trypsin/Lys-C mixture (Mass Spec Grade, Promega, Madison, WI, USA) according to the instruction manual ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... NanoGlo Live Cell Assay System substrate was then added and donor emission (450/8 nm BP filter) and acceptor emission (600 nm LP filter) were immediately measured on a GloMax Discover plate reader (Promega). BRET binding signal was defined as acceptor/donor ratio ...
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bioRxiv - Microbiology 2024Quote: ... 4 μg of each of the 3 HIV-envelope expression plasmids were co-transfected with 8 μg of backbone plasmid pSG3ΔEnv in HEK 293T cells using FuGene 6 transfection reagent (Promega, catalog # E5911). Virus was harvested 48 h post-transfection ...
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bioRxiv - Microbiology 2024Quote: ... PBMCs viability was confirmed using a CytoTox96 non-radioactive cytotoxicity assay kit (Promega). For cytokine quantification experiments ...
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bioRxiv - Microbiology 2024Quote: ... 12.5 µL GoTaq master mix (Promega, Madison, WI, USA) and 1 µL (10 ng µL−1 ...
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bioRxiv - Microbiology 2024Quote: ... and the luciferase signal was developed using the Nano-Glo luciferase assay system (Promega) per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Plasmids were isolated by Wizard® SV minipreps by protocol (Promega). GmPGIP gene sequences were confirmed against the original G ...
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bioRxiv - Plant Biology 2024Quote: ... 1 mM DTT) optimized for TEV protease cleavage (ProTEV Plus, Promega, V6101). Samples were adjusted to a final volume of 10 mL with 100U TEV protease and incubated at 4°C for overnight ...
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bioRxiv - Cell Biology 2024Quote: ... the GSH/GSSG-Glo Assay (Promega) was conducted according to the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then treated overnight with 2000 U/mL of universal Type I IFN (PBL Bioscience) and lysed at 24 h post treatment using Passive Lysis Buffer (Promega). Samples were processed and luciferase activity was measured using the Dual-Luciferase Assay System (Promega ...
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bioRxiv - Cell Biology 2024Quote: ... the CellTox Green assay (Promega) was performed ...
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bioRxiv - Cell Biology 2024Quote: Cell viability was assessed using the CellTiter-Glo luminescent cell viability assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and the luciferase activity was measured using the Dual-Luciferase reporter assay system (Promega). Measurements were acquired by a BioTek synergy HTX multimode reader (Agilent).
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bioRxiv - Cell Biology 2024Quote: ... 15 μl of methyl thiazolyl tetrazolium (MTT, Promega, Madison, WI, USA.) was added to each well and incubated for another 4 hours ...
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bioRxiv - Cell Biology 2024Quote: ... cell viability was measured using the RealTime-Glo™ MT Cell Viability Assay (Promega Cat. #G9713) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and further digested with trypsin (Promega) at an enzyme-to-substrate ratio of 1:50 (w/w ...
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bioRxiv - Cell Biology 2024Quote: ... the Firefly and Renilla Luciferase activity was measured in triplicate for each sample using the Dual-Luciferase Reporter Assay System (Promega, Australia). Luciferase activity was expressed as a ratio of Renilla to Firefly activity (Van Etten et al. ...
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bioRxiv - Cell Biology 2024Quote: ... LDH was measured using the CytoTox 96 Non-Radioactive Cytotoxicity Assay (Promega, cat. no. G1780). The protocols for BUN and LDH assay were modified to a 384 well microplate format with no efflux dilution.
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bioRxiv - Cell Biology 2024Quote: ... 8.62 µl ViaFect (Promega E4981) and 103 µl of Opti-MEM (Gibco 31985062) ...
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bioRxiv - Cell Biology 2024Quote: ... 20µL of CellTiter-96®Aqueous and One Solution MTS assay (Promega; #G3580) was added to each well using a multichannel pipette (Transferpette ...
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bioRxiv - Cell Biology 2024Quote: ... they were transfected with either control plasmid or Flag-GSK-3α WT human plasmid using Fugene 6 (Promega, #E2693) at a 3:1 ratio (DNA:Fugene 6) ...
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bioRxiv - Cell Biology 2024Quote: ... DNA was cleaned up with Promega Wizard SV gel and PCR Cleanup System (Promega). The inserts and the backbone were mixed in a 3:1 ratio and incubated with the 2x NEBuilder HiFi DNA assembly enzyme mix (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then suspended into 4mL of culture and Oregon Green HaloTag reagent (Promega #G2801) was added to a final concentration of 2uM ...
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bioRxiv - Cell Biology 2024Quote: ... Oligonucleotides encoding guide RNAs targeting M18BP1 (5’- TTGTACTGAAAAAATCATCA-3’) were cloned into pX459-v2 and co-transfected using FuGENE 6 (Promega) with pUC19 containing a 1528 base pair stretch containing the mutated sequence of the locus of interest and homology arms ...
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bioRxiv - Cell Biology 2024Quote: ... Trolox was purchased from Millipore Halo Tag ligand was purchased from Promega. Salmon sperm DNA was purchased from Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... RQ1 RNase-Free DNase (Promega, M6101) and clean-up performed using Monarch® RNA Cleanup Kit (Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were stained for 50 min with Janelia dye 646 HaloTag ligand (Promega, GA1120) and Hoechst 33342 ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins on the beads were digested by adding 200 ng of a trypsin/Lys-C mix (Promega) at 37°C overnight ...
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bioRxiv - Cell Biology 2024Quote: Goat polyclonal anti-luciferase was purchased from Promega (cat. G7451) and used for immunostaining and immunoblotting ...
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bioRxiv - Cell Biology 2024Quote: ... the reaction mixtures for in vitro transcription were treated with RQ-1 RNase-free DNase I (Promega). Synthesized mRNAs were treated with phenol–chloroform to remove protein components ...
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bioRxiv - Cell Biology 2024Quote: ... the 5′-end of each mRNA was capped using Ribo m7G Cap Analog (Promega), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... 20 µL of CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega #G3582) reagent was added to each well upon completion of experiment duration ...
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bioRxiv - Cell Biology 2024Quote: ... mRNAs were synthesized using RiboMAXTM Large Scale RNA Production Systems-T7 (Promega, Madison, WI, USA) using the corresponding plasmid as a template as previously described (Yamagata et al. ...