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Citations for Promega :
2501 - 2550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... membranes were reblotted to detect β-actin expression using primary (Cell Signal #3700) and secondary-HRP (Promega #W402B) antibodies and developed as described.
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bioRxiv - Microbiology 2024Quote: ... single transconjugant colonies were subjected to DNA extraction using Wizard Genomic DNA Purification Kit (Promega), and the DNA was used for PCR amplification using the PCRBIO HS Taq Mix Red polymerase ...
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bioRxiv - Immunology 2024Quote: ... cells were lysed with cell culture lysis reagent (Promega), and luciferase activity was measured by Centro XS3 LB 960 (Berthold).
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bioRxiv - Immunology 2024Quote: ... 50 μl of Bright-Glo Luciferase reagent (Promega E2620) was added to each well ...
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bioRxiv - Genetics 2024Quote: ... GoTaq Master Mix (Promega, Wisconsin, United States) was used according to manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... 1x or 2x TAE (Tris base, acetic acid, ethylenediaminetetraacetic acid; Promega) buffered agarose gel electrophoresis was used to separate the DNA ...
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bioRxiv - Microbiology 2024Quote: ... RNA was treated with DNAse I (Promega) to degrade contaminating DNA ...
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bioRxiv - Immunology 2024Quote: ... using the Steady-Glo® Luciferase Assay System (Promega Corporation) read on a Promega GloMax Discover machine ...
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bioRxiv - Cell Biology 2024Quote: Fugene 6 was obtained from Promega (E2691). Lipofectamine 2000 was obtained from Thermo-Fisher Scientific (11668019) ...
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bioRxiv - Neuroscience 2024Quote: ... Plates were incubated for 48 h and cell viability assay performed (Promega CellTiter-Glo 2.0). Luminescence was recorded using a SynergyH4 microplate reader (Agilent Technologies ...
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bioRxiv - Immunology 2024Quote: ... and cells were treated with 3µg/ml LPS O111:B4 and 0.25% (vol/vol) Fugene HD Plus (Promega) to cause transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... and digested with Lys-C (1/200 weight of protein; Fujifilm Wako) and sequencing grade modified trypsin (1/100 weight of protein; Promega, Madison, WI, USA) at 37 °C overnight in 0.1 % Rapigest ...
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bioRxiv - Cell Biology 2024Quote: ... using Fugene-HD reagent (Promega E2311). Three days later ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% Digitonin (Promega) and 50x EDTA-free Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing grade modified trypsin was purchased from Promega. All the MS grade solvents ...
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bioRxiv - Microbiology 2024Quote: ... were digested with a modified MS grade trypsin (Promega; 1:5 enzyme/protein) overnight at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were seeded onto acid-washed coverslips at a density of 3 × 104 cells per coverslip for 24 h before transfection with 1μg of plasmid DNA using 3 μl Fugene HD transfection reagent (Promega). HaloTagged AP-2 σ2 was visualized by adding Janelia Fluor 646-HaloTag ligand (Promega) ...
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bioRxiv - Cell Biology 2024Quote: ... HaloTagged AP-2 σ2 was visualized by adding Janelia Fluor 646-HaloTag ligand (Promega). Ligand (100 nM ...
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bioRxiv - Biophysics 2024Quote: ... 1% unmethylated Lambda DNA (Promega #D1521) was spiked into genomic DNA to monitor bisulfite conversion efficiency ...
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bioRxiv - Cancer Biology 2024Quote: ... CellTiter 96 non-radioactive cell proliferation assay kit (Promega, G4000) was used to determine cell growth and the manufacturer’s instructions were followed ...
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bioRxiv - Developmental Biology 2024Quote: ... Lysates were diluted 1:10 with Milli-Q water and processed for PCR using GoTaq G2 Flexi DNA Polymerase kit (Promega). Reactions were placed in a thermocycler on a touchdown program ...
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bioRxiv - Cell Biology 2024Quote: ATP levels in conditioned media and serum were measured by RealTime-Glo Extracellular ATP Assay kit (Promega, Madison, WI).
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bioRxiv - Neuroscience 2024Quote: ... Total RNA was extracted using the Reliaprep RNA Cell Miniprep System (Promega, Madison, WI, USA) and reverse transcribed using ReverTra Ace (Toyobo ...
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bioRxiv - Neuroscience 2024Quote: ... 200 ng of RNA was used for cDNA synthesis following Goscript Reverse Transcriptase (Promega #237815) protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... secondary antibodies: anti-rabbit HRP or anti-mouse HRP (Promega, 1:10000).
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bioRxiv - Molecular Biology 2024Quote: ... and 40 U/ml RNasin plus (Promega)) supplemented with 1% Triton X-100 and 0.5% Na deoxycholate ...
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bioRxiv - Molecular Biology 2024Quote: ... Firefly luciferase activity was measured using Dual-Luciferase Reporter Assay System (Promega, USA Cat#1910). The transfected cells were harvested in Passive lysis buffer (PLB ...
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bioRxiv - Cancer Biology 2024Quote: ... an equal volume of luciferase substrate (Bright-Glo, Promega) was added to each well ...
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bioRxiv - Bioengineering 2024Quote: ... The obtained PCR product was purified with the Wizard® SV Gel and PCR Clean-Up System (A928, Promega) and blunt end ligation was performed followed by electroporation of freshly made competent cells [36] to yield 3.6×107 total transformants ...
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bioRxiv - Cell Biology 2024Quote: cAMP levels were determined using the cAMP-GloTM Max Assay (Promega, Madison ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were digested overnight at 37°C with 0.2 μg trypsin (Promega). Formic acid was then added to 2% and the samples were incubated at 37°C for 30 min to cleave RapiGest ...
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bioRxiv - Microbiology 2024Quote: ... Cells were lysed 24 hours after transfection in passive lysis buffer (Promega) and firefly and Renilla luciferase activity were measured ...
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bioRxiv - Physiology 2024Quote: ... Reagents from the Promega GoTaq qPCR Master Mix kit (A6001, Promega) were used to perform qPCR ...
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bioRxiv - Cell Biology 2024Quote: ... S2 cells were transfected at 60% confluency with FuGENE® (Promega) with selected pUASTattB-based constructs together with pAct5c-GAL4 ...
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bioRxiv - Cancer Biology 2024Quote: ... and bioluminescence was measured with GloMax Explorer (Promega) plate reader.
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bioRxiv - Cancer Biology 2024Quote: ... caspase 3/7 activity was measured using Caspase-Glo® 3/7 Assay System- (Promega, G8091) per manufacture’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... CellTiter-Glo reagent (Promega) was mixed at a 1:1 ratio with cells and luminescence was measured on a Veritas luminometer at 0 ...
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bioRxiv - Molecular Biology 2024Quote: ... CellTiter-Glo reagent (Promega) was mixed at a 1:1 ratio with cells and luminescence was measured on a Veritas luminometer at 0 ...
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bioRxiv - Biochemistry 2024Quote: Constructs of nanobody variants were transformed into Single step (KRX) E.coli strain (Promega) at the end of molecular cloning ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed with TBST and incubated either with anti-mouse or anti-rabbit IgG horseradish peroxidase-conjugated secondary antibody (Promega, 1:10000). Chemiluminescence images were captured by the BioRad ChemiDoc detection system.
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bioRxiv - Cancer Biology 2024Quote: ... with QuantiFluor® ONE dsDNA dye (Promega, E4870). Temozolomide was purchased from Sigma-Aldrich (T2577).
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bioRxiv - Cell Biology 2024Quote: ... Renilla and Firefly luminescence was measured by Dual-Luciferase Reporter Assay (Promega, Hampshire, England) (Supplementary Methods) ...
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bioRxiv - Cell Biology 2024Quote: ... Luciferase assays were performed using the Dual Luciferase Reporter Gene Assay System (Promega) according to the instructions provided by the manufacturer ...
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bioRxiv - Physiology 2024Quote: ... Lysates were diluted using one volume of dilution buffer (10 mM Tris pH 8, 0.4% NP40, 5 mM CaCl2, 2 U/mL RQ1 DNAse (Promega)) and then incubated with anti-Flag or anti-p400 antibodies coupled to agarose beads (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... Viral titers were measured by infecting MDCK cells and measuring Nanoluciferase activity 8 h later with the Nano-Glo Luciferase Assay System (Promega)(52).
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Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2024Quote: Genomic fragment containing the murine Rorc 3’UTR was cloned into psiCHECK2 luciferase reporter plasmid (Promega). This construct was also used to generate the let-7 ʻseed’ deletion mutant derivative using the QuikChange Multi Site Mutagenesis Kit (catalog 200514-5 ...
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Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2024Quote: ... Reporter activity was detected with the Dual-Luciferase Reporter Assay System (Promega).
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bioRxiv - Cancer Biology 2024Quote: ... Luciferase activities were conducted 24 hours after transfection in the cells treated with or without the TEAD inhibitor CP1 using the dual-luciferase reporter kit (cat. E1910, Promega, Madison, WI, USA). Assays were conducted in triplicates and quantified using PerkinElmer EnVision plate reader ...
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bioRxiv - Genomics 2024Quote: ... 100 μL of CellTiter-Glo reagent (Promega) was then added to each well containing cell suspension ...
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bioRxiv - Microbiology 2024Quote: ... In vitro mRNA synthesis was then carried out at 37 °C for 4 h in T7 RiboMAX Large Scale RNA Production System kit (Promega) according to the manufacturer’s recommendations ...