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Citations for Promega :
2651 - 2700 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... the sample was digested with 400 ng trypsin (Trypsin Gold, Promega) at 37°C overnight ...
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bioRxiv - Genetics 2024Quote: ... dNTP mix (2.5mM each, Promega; U1515), ERCC RNA Spike-In Mix (1:3.2x107 Thermo Fischer Scientific 4456740 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 250 μM of each dNTP (Promega, Cat # R0192), 1mg/mL of bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2024Quote: ... These fragments were cloned into expression plasmids based on a pCI-neo mammalian expression vector (Promega, Madison, WI, U.S.A.), e.g ...
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bioRxiv - Microbiology 2024Quote: ... Quantitative PCR was performed using the GoTaq qPCR SYBR mastermix (Promega) on a LightCycler 480 instrument (Roche) ...
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bioRxiv - Immunology 2024Quote: ... FuGENE® was from Promega, while cholera toxin B (CTB ...
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bioRxiv - Molecular Biology 2024Quote: ... and corresponding controls were reverse transfected in HepG2 cells in triplicates as described above and a glucose uptake assay (Glucose Uptake-GloTM Assay, Promega, Madison, US) was performed ...
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Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: HEK293 cells growing at 70% confluency in a 10-cm dish were transfected with wild-type human NOPR or NOPLight (3 μg DNA) and GloSensor-20F (Promega, 2.5 μg DNA) using 12 μL Lipofectamine 2000 (Thermo Fisher ...
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bioRxiv - Cell Biology 2024Quote: ... RNase-free DNase from Promega, SUPERaseIn from Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... 50 μL IVT reactions were carried out for 2 hours at 30°C using commercially available Nuclease-Treated Rabbit Reticulocyte Lysate (RRL, Promega) and reaction components were added in the following order ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 µL of CellTiter-Glo reagent (Promega) were added to each well ...
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bioRxiv - Cell Biology 2024Quote: ... 30 μL of FuGENE HD (Promega) was added for every mL of DNA solution ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were digested using 0.005 µg/µl trypsin (sequencing grade modified trypsin; Promega) in 40 mM NH4HCO3/10% ACN at 4°C for 20 minutes followed by 16 h at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... and 40U/ml RNAsin plus (Promega). Lysates were sonicated using a Bioruptor (Diagenode ...
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bioRxiv - Cancer Biology 2024Quote: ... (G5421, Promega, Belgium), and CellTiter® 3D Cell Viability Assay (G9682 ...
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bioRxiv - Biochemistry 2024Quote: ... reduced and digested with trypsin (mass spectrometry grade; Promega Corporation) at a trypsin:protein ratio of 1:20 and peptides were purified using C18 tips (Pierce C18 Tips ...
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bioRxiv - Microbiology 2024Quote: ... Peptide digestion was initiated by adding 25 µL of 50 mM ammonium bicarbonate buffer (pH 8) containing 2 µg trypsin (Sequencing Grade Modified Trypsin, Promega, Cat. no. V5117) directly on top of the column and incubating overnight at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... Cell viability was assessed using CellTiter-Glo® Luminescent Cell Viability Assay (Promega, Cat. no. G7570) as per manufacturer’s instructions.
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bioRxiv - Developmental Biology 2024Quote: ... RiboMAX™ Large Scale SP6 RNA polymerase kit (Promega, #P1280) was used for in vitro transcription on XbaI linearized pCS2+ vectors ...
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bioRxiv - Cell Biology 2024Quote: ... and the EGFP sequence was replaced with the Halo7 sequence (Promega). The linker sequence 5’-ACCGGTGGTGGGCGCGCCTCTGGTGGCGGATCCGGGGGT-3’ was incorporated between CD63 ...
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bioRxiv - Cell Biology 2024Quote: ... The DNA was extracted using the Wizard Genomic DNA Purification Kit (Promega, Madison, USA), according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2024Quote: ... An amount corresponding to 5 % of the pull-down volume was saved for Western blot analyses and 1 µg of trypsin gold (Promega) was then added to the beads for 16 h at room temperature in a thermomixer at 600 RPM ...
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bioRxiv - Genomics 2024Quote: ... (34) DNA template and unused depletion probes were removed through addition of 4 μL RNase-free DNAse (Promega) with 5 μL 10 mM CaCl2 in 30 μL Nuclease-free water and incubated at 37 °C for 30 min ...
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bioRxiv - Systems Biology 2024Quote: ... Trypsin Gold (V5280, Promega) was added to a final concentration of 20 ng/µL ...
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bioRxiv - Genomics 2024Quote: The promoter of bovine RHO was cloned into a pGL3 luciferase reporter vector (Promega E1741). HEK293 cells were transfected with plasmids expressing the RHO reporter vector ...
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bioRxiv - Genomics 2024Quote: ... Luciferase activity was performed 48 hrs following ASO transfection with the Firefly Luciferase assay system (Promega E4030), following the manufacturer instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... genomic DNA was extracted from embryos using the Wizard Genomic DNA Purification Kit (Promega) according to the manufacturer’s Animal Tissue protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... or CellTiter-Glo Luminescent Cell Viability Assay (Promega) was used to measure cell viability ...
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bioRxiv - Cell Biology 2024Quote: Total accumulated cytotoxicity (an integrated readout for apoptosis and late apoptotic necrosis) was monitored in real-time using the Cell-tox assay (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were incubated in culture medium without phenol red with 0.1 mM D-luciferin (VivoGlo™ Luciferin, Promega, Madison, WI, USA) (experimental medium) ...
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bioRxiv - Cancer Biology 2024Quote: ... and were authenticated using the PowerPlex® Fusion System (Promega) performed at the Hartwell Center at St ...
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bioRxiv - Cancer Biology 2024Quote: ... 25 µL of CellTiter-Glo (Promega Catalog# G9241) was then added to each well to measure viability using a Multidrop Combi and the plates were incubated for an additional 25 min at room temperature.
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bioRxiv - Cell Biology 2024Quote: ... 22 μl of nuclease-free water) (Illumina, #20034197 and Promega, #G9441) at 37°C for 30 minutes with 300 rpm shaking ...
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bioRxiv - Cancer Biology 2024Quote: ... and CellTiter-Glo 2.0 assay kit (Promega; G9241), respectively ...
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bioRxiv - Cancer Biology 2024Quote: Gene expression analysis was performed using GoTaq qPCR SYBR mastermix (Promega), according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Transfection solutions were made at a lipid (µg) to plasmid (µL) ratio of 3:1 using FuGene 6 (Promega) as the transfection reagent ...
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bioRxiv - Cancer Biology 2024Quote: ... cell viability was measured using CellTiter-Glo 3D (Promega, Madison, WI, United States). The luminescence was acquired with BioTek Synergy H1 ...
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bioRxiv - Cancer Biology 2024Quote: ... Luminescence was measured with a GloMax 96 luminometer (Promega).
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bioRxiv - Cancer Biology 2024Quote: ... clonogenicity was assessed by measuring luminescence using CellTiter-Glo 2.0 reagent (Promega #G9242) and POLARstar Optima plate reader according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... A colorimetric assay (Cell Titer Blue Cell Viability Assay, Promega, Madison, WI, US) that detects cellular metabolic activities was used to assess cell viability ...
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bioRxiv - Cell Biology 2024Quote: ... Then each supernatant was transferred into a new tube before the revelation with either Luciferase Assay Reagent (Promega) or with β galactosidase (βGal ...
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bioRxiv - Cell Biology 2024Quote: ... The collected sEVs were incubated with 50-100 nM (final concentration) HaloTag TMR ligand (Promega) or HaloTag SaraFluor650T (SF650T ...
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bioRxiv - Cell Biology 2024Quote: ... The insert was then cloned in the pKH3 plasmid using classical restriction-ligation with HindIII and XbaI restriction enzymes (Promega, Madison, USA) and the final vector sequence was validated by Sanger sequencing (Eurofins Discovery).
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bioRxiv - Cell Biology 2024Quote: ... cells were lysed in Reporter Lysis Buffer (Promega) and processed according to manufacturer’s instruction ...
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bioRxiv - Cell Biology 2024Quote: ... The cloning was done by classical restriction-ligation using SalI and XbaI restriction enzymes (Promega). The final vector sequence was validated by Sanger sequencing (Eurofins Discovery).
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bioRxiv - Cell Biology 2024Quote: ... Gene cassettes were generated by sequential cloning of the relevant fragments in the pGEM-T plasmid (Promega), which served as template to PCR-amplify the relevant linear cassettes ...
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bioRxiv - Cell Biology 2024Quote: ... washed with ammonium bicarbonate (0.1M) and proteolyzed (0.4µg of LysC (Wako) for 3 hours and 1.2µg of Trypsin (Promega) overnight) ...
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bioRxiv - Cancer Biology 2024Quote: Cell viability assays were performed using CellTiter-Glo 2.0 (Promega). Apoptosis was measured using Caspase-Glo 3/7 (Promega) ...
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bioRxiv - Cancer Biology 2024Quote: ... Apoptosis was measured using Caspase-Glo 3/7 (Promega). Early apoptosis and late apoptosis/necrosis were measured using Alexa Fluor 488 Annexin V/Dead Cell Apoptosis Kit (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... reduced (6.5mM DTT, 1 hour at 60°C), alkylated (54mM Iodoacetamide, 30min, RT) and digested with 1µg of Trypsin (Promega) (96) ...