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Citations for Promega :
1 - 50 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... PCR products were sub-cloned into the pGEM-T Easy vector (Promega, A1360) and sequenced ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 μl of CellTiter-Blue (Promega) was added and cells were incubated for 90 minutes at 37°C before measuring fluorescence using an EnVision plate reader (PerkinElmer) ...
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bioRxiv - Cancer Biology 2024Quote: ... and luciferase activity was measured in triplicate using the dual luciferase reporter kit (Promega) and a Glo-Max 96 Multidetector system (Promega) ...
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bioRxiv - Plant Biology 2024Quote: ... Following by reverse transcription to cDNA using the GoScript Reverse Transcription System (Promega, A5001). Real-time quantitative PCR (RT-qPCR ...
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bioRxiv - Plant Biology 2024Quote: ... Real-time quantitative PCR (RT-qPCR) was performed using the GoTaq QPCR Master Mix (Promega, A6001) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... 54] and trypsin (sequencing grade, Promega) was added in an enzyme to protein ratio 1:50 for overnight digestion at 37°C ...
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bioRxiv - Plant Biology 2024Quote: ... 4 µl of the RNA from input and supernatant and 7 µl of the pre-eluates and eluates were digested with RQ1 DNase I (Promega, Walldorf, Germany) and cDNA synthesis was carried out with Superscript IV reverse transcriptase (Thermo Fischer ...
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bioRxiv - Physiology 2024Quote: ... (Promega, W4011).
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bioRxiv - Neuroscience 2024Quote: ... Supernatant was removed and pellet was resuspended in 400 µL of sort buffer (1 mM EDTA 0.2 U/µL RNase inhibitor (Promega, N211B), 2% BSA (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... and 0.2 U/µL RNase inhibitor (Promega, N211B), 2% BSA (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclei were resuspended in 35 µL of reaction buffer (0.2 U/µL RNase inhibitor (Promega, N211B), 2% BSA (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... and RNasin Plus RNase Inhibitor (Promega N2611) at 60 U/mL for RNA experiments ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... and nanoluc substrate (Promega, 1:200) diluted in 1X passive lysis buffer (Promega) ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... diluted in 1X passive lysis buffer (Promega). Luminescence of the fractions was measured with a luminometer (Promega ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... Luminescence of the fractions was measured with a luminometer (Promega, GloMax Explorer). EV associated peak fractions were 2-fold serially diluted in PBS and analyzed by nanoparticle tracking analysis (Nanosight NS300 ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... Fractions were complemented using LgBiT (Promega, 1:200 dilution) and nanoluc substrate (Promega ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... Luminescence of the fractions was measured with a luminometer (Promega, GloMax Explorer). EV-associated HiBiT-Arc was identified in gradient fractions 15-17 as these fractions have a density consistent with the known densities of EVs (1.10-1.15g/mL) ...
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bioRxiv - Neuroscience 2024Quote: ... After cDNA conversion with a GoScript™ Reverse Transcriptase kit (#A5001, Promega), quantitative real-time PCR was performed on a Bio-rad CFX96 Real-time system ...
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bioRxiv - Plant Biology 2024Quote: ... following the instructions of the manufacturer (Promega). Two nanograms of cDNA were used as template in each qRT-PCR ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells were lysed with 0.05% aqueous SDS and luminescence was measured using the GloMax 20/20 Luminometer (Promega) [15].
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bioRxiv - Neuroscience 2024Quote: ... corresponding HRP-conjugated secondary antibodies (Anti-Rabbit, #W4011 and Anti-Mouse #W4021, both from Promega) were incubated (1:4,000 ...
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bioRxiv - Neuroscience 2024Quote: ... and digested with trypsin (sequencing grade, Promega) at 1:100 (enzyme ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting bands were gel purified and cloned into pGEM T-easy (Promega) and sent out for Sanger sequencing.
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bioRxiv - Neuroscience 2024Quote: ... and 50U/ml of RNasin (Promega, cat# N2611), centrifuged and nuclei were resuspended in 1 ml of washing buffer ...
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bioRxiv - Plant Biology 2024Quote: Steady-state kinetic analyses were performed in triplicate using the UDP-Glo Glycosyltransferase Assay kit (Promega) to monitor the released UDP according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... and incubated for 1 h at room temperature before measuring luminescence using a GloMax Explorer plate reader (Promega). A standard curve was used for quantification of the UDP produced ...
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bioRxiv - Plant Biology 2024Quote: ... or the MagneHis Protein Purification System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... The soluble GST and His fusion proteins were purified using the MagneGST Protein Purification System (Promega, USA) or the MagneHis Protein Purification System (Promega ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins captured on magnetic beads were digested with 1.5 mg/mL trypsin (Promega Cat# V5111) in 50 mM ammonium bicarbonate overnight at 37 °C ...
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bioRxiv - Plant Biology 2024Quote: ... RNA extraction was performed using an RSC 48 RNA extraction robot (Maxwell) and the Maxwell RSC Plant RNA kit (Promega). Although the RNA yield for some of the 64 samples was low ...
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bioRxiv - Plant Biology 2024Quote: ... Magne Halo Tag Beads (Promega) were used to purify and capture the produced protein ...
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bioRxiv - Plant Biology 2024Quote: ... according to the instructions: stock oligo(dT)15 primer (C1101, Promega) concentration was 100 ng/μl ...
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bioRxiv - Plant Biology 2024Quote: ... TUNEL assay is performed using the DeadEnd Fluorometric TUNEL System (Promega). Following incubation ...
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bioRxiv - Plant Biology 2024Quote: ... The secondary PCR product was cloned in pGEMT-Easy vector (Promega Madison, USA), and Sequencing was performed using the nos promoter’s AP2 and 5’ junction (Eurofins Genomics India Pvt ...
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bioRxiv - Plant Biology 2024Quote: ... PCR products were purified for cloning into the pGEMT vector (Promega, Madison, WI, USA). A minimum of 10 clones per PCR product were sequenced to characterize the edited alleles in the transgenic plants.
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bioRxiv - Neuroscience 2024Quote: ... and the luminescence was quantified by the GloMax Luminometer plate reader (Promega).
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bioRxiv - Neuroscience 2024Quote: The CellTiter-Glo assay (Promega; Madison ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was extracted from FACS purified iPSC-neurons and frozen mouse brain tissue using the ReliaPrep RNA cell kit and ReliaPrep RNA tissue extraction kit (Promega) respectively.
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bioRxiv - Pathology 2024Quote: ... Lys-C (Promega) was added in an enzyme:substrate ratio of 1:75 for 4 hours at 25 °C ...
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bioRxiv - Neuroscience 2024Quote: ... The luminescence readings were acquired using a GloMax Microplate Reader (Promega). The percentage of viability was determined by normalizing the raw luminescence values to those of the vehicle condition (DMSO-treated wells ...
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bioRxiv - Neuroscience 2024Quote: ... viability was assessed with an ATP-based chemiluminescent assay (Cell Titer-Glo®, Promega; Cat#G7570) following the manufacturer’s instructions ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... plated in 6-well dishes were centrifuged at 1000xg for 10 minutes and suspended in 5x Passive Lysis buffer (Promega). Each data point represents an individual well ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... and nanoluc substrate (Promega, 1:200) diluted in 1X passive lysis buffer (Promega) ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... Arc-Hibit signal was identified by complementation with LgBiT (equal volumes) and measurement of Nanoluc-mediated luciferase [JDS1] (Nano-Glo Luciferase Assay, Promega, 565nm) on a microplate reader (SpectraMax iD3).Total Arc in the cell lysate is calculated by dividing the luciferase value of Arc cell lysate by the % of Arc lysate sample used from total sample collected ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were visualized with AttoPhos (Promega) and scanned with an FLA-5000 fluorescent image analyzer (Fujifilm) ...
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Activation of XBP1s attenuates disease severity in models of proteotoxic Charcot-Marie-Tooth type 1BbioRxiv - Neuroscience 2024Quote: ... GoTaq qPCR Master Mix (Promega, Madison, WI, USA). Gene expression changes were normalised to 36B4 or Ppia gene expression by using the ΔΔCT method.
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bioRxiv - Synthetic Biology 2024Quote: Purified NanoLuc protein (Promega REF G9711) was diluted to 10 µM in 0.1 mg/ml BSA solution ...
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bioRxiv - Synthetic Biology 2024Quote: ... using the Nano-Glo Luciferase Assay system (Promega REF N1110), by dispensing the Nano-Glo assay reagents at an equal volume to the protein synthesis reaction.
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then washed 3x with TBS-T and incubated for 2 hours at room temperature with either anti-rabbit (1:5000, Promega W4011) or anti-mouse HRP (1:5000 ...
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bioRxiv - Molecular Biology 2024Quote: ... A 1 μg aliquot of trypsin (Promega) was added to each 100 μg preparation of reduced and alkylated proteins and incubated at 37℃ for 18 h ...