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Citations for Promega :
1 - 50 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The proteins were digested overnight at 37ºC in 1.5 M urea and 66 mM ammonium bicarbonate with modified trypsin (Promega) at a 1:50 (M/M ...
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bioRxiv - Microbiology 2024Quote: Measurement of ATP content in mother cell was performed using the BacTiter-Glo Microbial Cell Viability Assay (Promega). As guided by the instructions ...
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bioRxiv - Microbiology 2024Quote: ... followed by reverse transcription to complementary DNA (cDNA) using 30 enzyme units(U) of the Moloney Murine Leukemia Virus reverse transcriptase (MMLV-RT) (Promega, WI, USA). The extension products were then analysed using the ABI3730xl DNA Analyzer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... The presence of released LDH in the supernatant was assessed using the Cytotox 96 Non-Radioactive Cytotoxicity Assay (Promega, G1780). The extended protocol can be found in the supplementary materials.
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bioRxiv - Microbiology 2024Quote: ... 50 µL of NanoGlow substrate (Promega) was added to each well before measuring in a luminometer (MicroLumat Plus ...
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bioRxiv - Microbiology 2024Quote: ... Luminescence was measured using Bright-Glo Luciferase Assay System (Promega, Madison, WI, USA) in black 96-well plates using VICTOR® Nivo™ Plate Reader (PerkinElmer ...
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bioRxiv - Microbiology 2024Quote: ... Flp-In T-REX HeLa cells were transfected with 1.8 μg of pOGG4 plasmid and 200 ng of pcDNA5/FRT/TO plasmid encoding the relevant transgene cassette using FuGENE HD transfection reagent (Promega). Cells with a stably integrated transgene were selected with 150 μg/mL hygromycin for 4-5 days ...
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bioRxiv - Microbiology 2024Quote: ... baylyi ADP1 was first digested with RQ1 DNase (Promega, WI, USA), followed by reverse transcription to complementary DNA (cDNA ...
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bioRxiv - Microbiology 2024Quote: ... and the luciferase activity was measured using the dual-luciferase reporter assay (Promega, cat# E1910). For experiments involving the transfection of viral proteins (Fig ...
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bioRxiv - Microbiology 2024Quote: ... The Dual-Glo Luciferase Assay System (Promega) was used to determine the relative activity of firefly luciferase to Renilla luciferase ...
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bioRxiv - Microbiology 2024Quote: ... 20 µg of the plasmid was co-transfected with 2.5µg of pGL4.75 Renilla luciferase control vector (Promega) into DT40 AIDR/puro UNG-/- cells ...
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bioRxiv - Immunology 2024Quote: ... Lactate in flow-through medium was quantified using the L-lactate assay kit (Biomedical Research Service & Clinical Application, #A-108) and GloMax microplate reader (Promega) as suggested by the manufacturer ...
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bioRxiv - Immunology 2024Quote: ... 18-24 hours later the supernatant was harvested for cytotoxicity evaluation by measuring LDH release with CytoTox 96 nonradioactive cytotoxicity assay kit (Promega, #G1780), followed the protocol suggested by the manufacturer.
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bioRxiv - Biochemistry 2024Quote: ... 3ng/μL trypsin (Trypsin Gold, V5280, Promega, USA) and 0.01% enhancer (ProteaseMAX ...
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bioRxiv - Immunology 2024Quote: ... AP20187 dimerizer was added in cell culture media to a final concentration of 500 nM for 5 hours before taking the supernatants for LDH assay (G1780, Promega, Madison, WI). Cell death was measured on a scale with the media without cells set to 0% and cells lysed for 1 hour in 1% Triton X-100 set to 100%.
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bioRxiv - Biochemistry 2024Quote: ... and 0.01% enhancer (ProteaseMAX, V2071, Promega, USA) and 1 % DMSO) ...
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bioRxiv - Immunology 2024Quote: ... cDNA was generated with the ImPromII reverse transcription system (Promega #A3800). Quantitative PCR was performed using PerfeCTa qPCR FasMix II (QuantaBio #95119 ...
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bioRxiv - Immunology 2024Quote: ... 7.5% PEG-8000 (Promega, #V3011), 2 U/µL Maxima H-minus reverse transcriptase ...
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bioRxiv - Immunology 2024Quote: ... and reverse-transcribed by GoScript reverse transcription system (Promega). The qRT-PCR mixture consisted of 1 µL of cDNA template ...
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bioRxiv - Immunology 2024Quote: ... The CellTiter-Glo 2.0 assay (Promega) was then used to quantify ATP as a proxy for cell viability at 24h ...
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bioRxiv - Immunology 2024Quote: ... then lysed with Lysis Buffer (Promega) for 15 min on mechanical horizontal rotator ...
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bioRxiv - Microbiology 2024Quote: ... The proteins were resuspended in 100 mM NH4HCO3 and digested with trypsin (Promega, UK) at an enzyme-to-substrate ratio of 1:20 and protease max 0.1% (Promega ...
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bioRxiv - Microbiology 2024Quote: ... at an enzyme-to-substrate ratio of 1:20 and protease max 0.1% (Promega, UK). Digestion was carried out overnight at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: Luciferase reporter with minimal promoter (pGL4.23, Promega) and Renilla luciferase plasmid (pGL4.74 ...
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bioRxiv - Cancer Biology 2024Quote: ... The luciferase assays were carried out with Dual-Luciferase Reporter Assay System (E1910, Promega)
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bioRxiv - Cancer Biology 2024Quote: ... The plates were incubated for 72h before the CellTiter-Glo® 2.0 Cell Viability Assay (Promega: G924A) was performed following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Core Kit (Promega, Wisconsin, USA), following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Authenticity of cell lines was evaluated by detection of ten genetic loci using the GenePrint® 10 System (Promega) and cross referencing to ATCC or internal genetic profiles.
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bioRxiv - Cancer Biology 2024Quote: ... Analyses were carried out using Promega PCR master mix (Promega) on an LC-480 Real-Time PCR system (Roche) ...
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bioRxiv - Cancer Biology 2024Quote: ... we quantified the luciferase and Renilla signals using the dual-luciferase reporter gene assay kit (Promega, E1960), following the protocol provided by the manufacturer.
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bioRxiv - Cancer Biology 2024Quote: ... and Renilla luciferase plasmid (pGL4.74, Promega) were co-transfected into 293T cells ...
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bioRxiv - Cancer Biology 2024Quote: ... and 0.01% Digitonin (Promega #G9441) and incubated on ice for 3 min ...
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bioRxiv - Cancer Biology 2024Quote: Glutathione was measured using the GSH-Glo Assay from Promega (V6911) according to the manufacturer protocol ...
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bioRxiv - Cancer Biology 2024Quote: Total cellular ATP levels were measured using the CellTiter-Glo® Luminescent Cell Viability Assay kit (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and the GSH/GSSG ratio were measured using the GSH/GSSG-Glo assay kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and NADP/NADPH-Glo Assay kit (Promega), respectively ...
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bioRxiv - Cancer Biology 2024Quote: ... the ROS-Glo H2O2 Assay Kit (Promega #G8820) was used according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Cellular NAD+/NADH and NADP+/NADPH ratios were measured using the NAD/NADH-Glo Assay (Promega) and NADP/NADPH-Glo Assay kit (Promega) ...
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bioRxiv - Cancer Biology 2024Quote: ... following intraperitoneal injections of 150 mg/kg D-luciferin (Promega, Alexandria, NSW, Australia) and under anaesthesia with isoflurane ...
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bioRxiv - Cancer Biology 2024Quote: In cellulo Rluc-RNA reporter assays were performed using the Dual-Luciferase Reporter Assay System Kit (Promega) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2024Quote: ... and ReliaPrep RNA Tissue Miniprep System (Z6111, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... on a Maxwell® 16 MDx (Promega). RNF43 exome sequencing was performed using a RNF43 Custom Panel (Illumina) ...
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bioRxiv - Cancer Biology 2024Quote: ... and ReliaPrep RNA Tissue Miniprep System (Z6111, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Genomic DNA was isolated from the 102 human FFPE colorectal tissue samples using the Maxwell® RSC Blood DNA Kit (Promega) on a Maxwell® 16 MDx (Promega) ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were incubated with 0.5x CellTox Green cytotoxicity dye (Promega, G8741), cisplatin (obtained from the Clinical Pharmacy Services ...
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bioRxiv - Cancer Biology 2024Quote: ... a multiplexed caspase/viability assay was performed as described before [5] using the Multiplex Assay ApoLive-Glo (Promega, G6411) kit.
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bioRxiv - Cancer Biology 2024Quote: ... senescent and diapause-like SK-Mel-103 and A549 cells using CellTiter-Glo Luminescent Cell Viability Assay (Promega, #G7571) following manufacturer’s instructions or Crystal Violet (Merck ...
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bioRxiv - Cancer Biology 2024Quote: Quantitative real-time PCR (RT-qPCR) was performed using GoTaq PCR Master Mix (Promega, #A6002) and specific primers listed below ...
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bioRxiv - Cancer Biology 2024Quote: ... The CellTiter-Glo Luminescent Viability Assay (Promega G7571) was used to measure cell viability at 24 ...
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bioRxiv - Cancer Biology 2024Quote: ... 20 mg of tissue was lysed with Passive Lysis 5X Buffer (Promega #E1941), and the lysate was incubated with H2O2 Substrate Solution at room temperature for 1 hour ...