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Citations for Promega :
1901 - 1950 of 2370 citations for DNA Sequencer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Each PCR product was digested with XhoI and BamHI and ligated with the T4 DNA ligase (Promega, Cat #: M1794) into a XhoI and BamHI pre-digested pcDNA3.1-mCherry-HIS vector ...
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bioRxiv - Plant Biology 2023Quote: ... The size of the amplified PCR products was ascertained by utilizing a 100 bp GeneRulers™ DNA ladder (Promega). PCR products were sent to Genewiz/ Azenta company for DNA sequencing in both directions.
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bioRxiv - Cell Biology 2023Quote: ... cells were plated on culture plates and transfected the next day with DNA constructs using FugeneHD reagent (#E2311, Promega), according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... Cells were seeded onto acid-washed coverslips at a density of 3 × 104 cells per coverslip for 24 h before transfection with 1μg of plasmid DNA using 3 μl Fugene HD transfection reagent (Promega). HaloTagged AP-2 σ2 was visualized by adding Janelia Fluor 646-HaloTag ligand (Promega) ...
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bioRxiv - Genomics 2023Quote: ... NGS of nanobiopsied mitochondria was compared to lysate obtained from single-cells collected by LCM (29,50) and control genomic DNA (gDNA) (Promega), sequenced using conventional sequencing methods (13).
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bioRxiv - Molecular Biology 2023Quote: ... Based on the migration of the amplified bands in relation to the industry-standard 100-bp DNA ladder (Promega), the size of the bands was further estimated (in nucleotide base pairs).
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bioRxiv - Molecular Biology 2023Quote: ... 1μl of the cDNA was used for PCR amplification reactions using using GoTaq® G2 DNA Polymerase (Promega, #M7845) in the standard 25µl reaction following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... a qPCR was performed using 40 ng digested genomic DNA as template with the GoTaq qPCR master mix (Promega) according to manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... 500 ng of plasmid DNA encoding for SthCsm-complex and guide RNA was mixed with 1.5 μL of FuGENE HD reagent (Promega), incubated for 15 min ...
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bioRxiv - Bioengineering 2023Quote: ... DNA libraries were used as templates for in vitro transcription by RiboMAX Large-scale RNA production system-T7 (Promega). The reaction mixture was incubated at 37°C for 2 h ...
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bioRxiv - Genomics 2023Quote: ... The platform also permits a high-throughput automated genomic DNA isolation from 48 samples in 40 minutes (Promega, 2020) making this system compatible with larger sample sets.
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bioRxiv - Cell Biology 2023Quote: DNA was prepared from bacterial cultures grown at 37°C using a Midiprep kit (PureYield Plasmid Midiprep System, Promega) according to the manufacturer’s instructions ...
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The tetrapeptide sequence of IL-1β regulates its recruitment and activation by inflammatory caspasesbioRxiv - Immunology 2023Quote: ... the indicated plasmids were mixed with a control RFP vector to a total of 1.0 μg DNA in 65 μL Opti-MEM and transfected using FuGENE HD (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... Protein was precipitated by the addition of 200 μl Protein Precipitation Solution (Promega Wizard HMW DNA Extraction Kit A2920). Using a wide bore tip the samples were mixed by drawing up contents from the bottom of the tube and then expelled on the side of the tube 5 times ...
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bioRxiv - Microbiology 2023Quote: ... Total genomic DNA was isolated from overnight cultures of Ag1 and Ag2 using the Genome Wizard kit (Promega, WI), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... Reference allele enhancer sequences for hZRS and hs737 were obtained via PCR cloning from human genomic DNA (Promega, G304A). Primers used for each enhancer sequence are outlined in Table S2 ...
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bioRxiv - Plant Biology 2023Quote: ... gRNA-targeted regions were amplified from genomic DNA isolated from the T1 plants with GoTaq Green Master Mix (Promega) using the primers g1-seqF/g1-seqR and g2-seqF/g2-seqR for gRNA1 and gRNA2 ...
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bioRxiv - Molecular Biology 2023Quote: All amplification except for use in cloning was performed with GoTaq® G2 DNA Polymerase (Promega Corporation, Madison, USA) following manufacturer’s instructions and buffers ...
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bioRxiv - Cancer Biology 2023Quote: IDH1 and NNT promoter sequences were amplified from genomic DNA of human primary melanocytes by PCR and cloned into pGL4.12 (Promega). Mutagenesis of E-boxes was performed using a QuikChange Site-Directed Mutagenesis Kit (Stratagene) ...
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bioRxiv - Biochemistry 2024Quote: Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (E1910, Promega corporation, Madison, WI), and subsequently used to infect suspension cultures of Sf9 cells (2 × 106 cells/ml ...
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bioRxiv - Immunology 2023Quote: Transient transfection was performed using 1 or 2 µg plasmid DNA and 3.5 or 7 µl FuGENE HD transfection reagent (Promega) per 5x 105 cells ...
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bioRxiv - Molecular Biology 2023Quote: ... S0ng of parental plasmid was amplified with complementary 35nt-long forward and reverse primer pairs (0.25uM each) containing the desired nucleotide substitution (at nt position 17) in the presence of 1.25U Pfu DNA polymerase (Promega) and 200uM dNTPs (each ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The plasmid DNA was purified from 4-mL overnight cultures by use of the Wizard Plus MiniPrep kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... the indicated plasmids were mixed with an empty vector to a total of 2.0 μg DNA in 125 μL Opti-MEM and transfected using FuGENE HD (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... were transfected 2 days after plating with 1 μg of total DNA with FuGENE 6 transfection reagent (E2691; Promega) at a ratio 1:3.
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bioRxiv - Microbiology 2024Quote: ... Cells were then transfected with plasmid DNA (4µg per plasmid per flask) using FuGENE HD (1:3 ratio; Promega) and cultured at 28°C.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Total RNA was purified of genomic DNA contamination using DNase (RQ1 RNAse-free DNAse, Cat. # M610A, Promega, Madison, WI) and reverse transcribed using the High Capacity cDNA Reverse Transcription Kit (Cat.# 4368814 ...
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bioRxiv - Molecular Biology 2024Quote: ... Contaminating genomic DNA was removed by RQ1 DNase (M6101) treatment according to the manufacturer’s instructions (Promega Corporation, WI USA). RNA concentration was determined using Qubit (Q32855 ...
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bioRxiv - Neuroscience 2023Quote: ... DNA were purified using the Wizard® SV Gel and PCR Clean-Up System protocol (Promega, Madison, Wisconsin, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Purification and assembly of DNA fragments were performed using a Wizard SV Gel and PCR Clean-up System (Promega) and a NEBuilder HiFi DNA assembly cloning kit (New England Biolabs) ...
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bioRxiv - Immunology 2024Quote: Extracellular DNA release was quantified from the supernatant of NETosis-stimulated neutrophils using QuantiFluor dsDNA system (Promega; Madison, WI) per manufacturer instructions ...
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bioRxiv - Synthetic Biology 2024Quote: DNA purification from agarose gel was done using Wizard SV Gel and PCR Clean-Up System Kit from Promega as per manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2024Quote: DNA purification from PCR mixture was done using Wizard SV Gel and PCR Clean-Up System Kit from Promega as per manufacturer’s instructions.
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bioRxiv - Plant Biology 2020Quote: ... PCRs were used to assay for wild-type or T-DNA insertion alleles in 20 μL reactions using GoTaq Green Master Mix (Promega) using manufacturer’s instructions and 30 cycles as follows ...
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bioRxiv - Biochemistry 2021Quote: ... Genomic DNA was eliminated by incubating 10 μL of isolated RNA with 1 U of RQ1 RNase-free DNase I (Promega) for 30 min at 30°C ...
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bioRxiv - Biochemistry 2021Quote: ... Genomic DNA was eliminated by incubating 2 μg of RNA with 2 U of RQ1 RNase-free DNase I (Promega) for 30 min at 30°C ...
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bioRxiv - Microbiology 2021Quote: ... The resulting libraries were checked for their quality using the High-sensitivity DNA chip using the Agilent 2100 Bioanalyzer (Waldbroon, Germany) and quantified using the QuantiFluor One dsDNA kit (Promega). Paired-end (2x300bp ...
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bioRxiv - Microbiology 2021Quote: Promoter fusions to lacZ were constructed by amplification of promoter regions from genomic DNA with the specified primers (Table S4) and ligated into pGEM-T Easy (Promega) for amplification and collection ...
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bioRxiv - Genetics 2021Quote: ... a 599 bp long construct was PCR amplified from genomic DNA isolated from the tail of a Pahenu2 homozygous animal using GoTaq G2 HotStart Green Master Mix (Promega). Primers are listed in Suppl ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and from the interior tissue (at least 2 mm away from the outermost edge of the specimen) using the Wizard Genomic DNA Purification Kit (Promega), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... DNA fragments of approximately the correct size were extracted from 1% agarose gels and ligated into the pGEMT-Easy (Promega) subcloning vector ...
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bioRxiv - Genomics 2020Quote: DNA was extracted from the fin clips of the challenged fish using a commercial kit (Wizard Genomic DNA Purification Kit, Promega), following the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... instrument was configured with the Standard Elution Volume (SEV) hardware and using the Maxwell 16 Cell DNA purification kit (Promega). For total RNA isolation ...
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bioRxiv - Neuroscience 2021Quote: Radioactive U1 and U7 snRNAs were generated by run-off transcription with T7 polymerase from linearized plasmid DNA templates in the presence of m7G cap analog (Promega) using the MEGAshortscript T7 kit (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: Preserved tissue (10-15 mg) was used for nucleic acid purification by employing the Wizard genomic DNA kit (Promega, USA) and following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: DNAseI treated total RNA from mESCs was used to synthesize complementary DNA (cDNA) using random primers (Promega Cat no. C1181), dNTP mix (LAROVA Cat no ...
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bioRxiv - Molecular Biology 2020Quote: ... ATFS-11-100(R/R)::GFP and Su91-69::GFP) were synthesized in reticulocyte lysate in the presence of [35S]methionine (T7 Quick for PCR DNA, Promega). Import into isolated mitochondria was performed in import buffer (3 % (w/v ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmids were eluted with RNase free water and used as templates for in vitro gene expression (E. coli S30 Extract System for Circular DNA, Promega).
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bioRxiv - Molecular Biology 2019Quote: ... of linearized DNA were used as templates for in vitro transcription in the T7 RiboMAX Large Scale RNA Production System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA was extracted from selected clones as well as in the parental clone using the Wizard Genomic DNA Purification Kit (Promega). NGS was performed using Nextera XT DNA Library Preparation kit and the NextSeq 500 sequencing systems (Illumina ...