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Citations for Promega :
1751 - 1800 of 2370 citations for DNA Sequencer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... Wizard Mini-Preps Plasmid DNA purification and SV Gel Clean-up kits were purchased from Promega (MyBio, Ireland). The O2-sensitive phosphorescent probe Pt-Glc was synthesized as described before [82] ...
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bioRxiv - Cell Biology 2021Quote: ... 2mL of Sf9 cells at 0.5×106 cells/mL were transfected with 2µg of fresh bacmid DNA and FuGene HD transfection reagent (Promega) at a 3:1 transfection reagent to DNA ratio according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... The identity of all cell lines was confirmed by DNA fingerprinting analysis utilizing the GenePrint 10 System (Promega).
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bioRxiv - Zoology 2021Quote: ... Sections were digested in a mixture of 97% Nuclei Lysis Solution (Promega; from the Wizard DNA isolation kit) and 3% 20mg/ml Proteinase K (Promega) ...
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bioRxiv - Neuroscience 2020Quote: ... cells were plated in 24-well plates and transfected with 0.25 μg plasmid DNA and 1.25 μL FuGENE (Promega). Primary neuronal culture was prepared as described previously (40) ...
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bioRxiv - Genomics 2021Quote: ... The RNA was separated via phenol-chloroform extraction and any residual DNA was degraded with RQ1 DNase (Promega). To further purify the RNA ...
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bioRxiv - Neuroscience 2020Quote: One µl of bisulfite-converted DNA was subjected to PCR using the G2 Green Mix (Promega, Mannheim – Germany) with bisulfite-specific primers at temperatures and concentrations as indicated in Appendix 3 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 4 hours after plating cells were transfected with 1μg of histone H3.3-HaloTag®Fusion Vector DNA (Promega) + 0.1 μg of NSD2 PWWP1-NanoLuc® Fusion Vector DNA (C-terminal ...
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bioRxiv - Microbiology 2020Quote: ... The KAN PCR amplicon with compatible ends was ligated into the plasmid backbone using T4 DNA ligase (Promega). The resultant pEXP5/Kan vector was linearized using XbaI and Butters gp31FLAG was ligated into the plasmid for transformation into chemically competent BL21 cells ...
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bioRxiv - Microbiology 2022Quote: ... Ten nanograms of template DNA was added to 5 µl Green GoTaq Reaction Mix Buffer (Promega, Madison, WI), 1.25 units GoTaq DNA Polymerase (Promega ...
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bioRxiv - Microbiology 2022Quote: ... virus was removed and cells were transfected with 2 µg of plasmid DNA pUCSP-A24Rcd using FuGeneHD (Promega) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... and the amplified DNA fragments were purified using Wizard SV Gel and PCR Clean-Up System (Promega #A9282). DIG-labelled RNA probes were synthesized using T7 or SP6 RNA polymerase and DIG RNA labeling Mix (Roche #11277073910) ...
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bioRxiv - Cell Biology 2022Quote: ... pH 8.0) with 2-5µg plasmids pre-digested by XcmI and 50 µg of herring sperm carrier DNA (Promega), then 700 µL of yeast transformation buffer (40% (w/v ...
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bioRxiv - Biochemistry 2021Quote: Linear DNA templates were generated by PCR using appropriate primers and transcribed into mRNA with T7 polymerase (Promega). In vitro translation was performed in rabbit reticulocyte lysate (Promega ...
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bioRxiv - Microbiology 2021Quote: Forty-nine confirmed Bp isolates were subjected to DNA extraction using the Wizard Genomic Extraction Kit (Promega, USA). For whole genome sequencing (WGS) ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid DNA was isolated from each clone using Wizard® Plus SV Miniprep kit (Promega, Madison, WI, USA) and a total of 72 individual clones were sequenced with pJET1.2-F and pJET1.2-R primers at Eurofins Genomics Germany GmbH.
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bioRxiv - Microbiology 2020Quote: ... duplicate wells were transfected with 1 µg HDM_Spike_RBD_B7-1 plasmids and 1 µg of Transfection Carrier DNA (Promega, E4881) using BioT reagent (Bioland Sci ...
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bioRxiv - Genetics 2022Quote: ... The DNM1 amplification was performed with specific primers pair (DNM1_Forward 5’-GGGTCTTGTACGGAGCAGGG-3’ and DNM1_Reverse 5’-GAGTCAGATAGTAAGGGCAAGCAC-3’) using Pfu DNA polymerase (Promega). After the first amplification to select DNM1 fragment of 761 bp ...
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bioRxiv - Genetics 2022Quote: ... clones with the correct insert size were isolated using the Wizard Plus SV Minipreps DNA Purification System (Promega) and sequenced to verify their identity ...
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bioRxiv - Microbiology 2022Quote: ... which was performed at Baseclear with the Wizard HMW DNA Extraction kit (Promega Benelux B.V., Leiden, The Netherlands). The genome of the fast-growing isolate Pfast was sequenced using Illumina Novaseq and ONT GridiON at Baseclear (Leiden ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Samples were treated with RNAse before extraction and DNA concentration was measured using the Quantifluor dsDNA system (Promega). For each control treatment (1/8 MIC and no antibiotic ...
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bioRxiv - Cancer Biology 2022Quote: ... The first step of PCR was carried out with 1 µl of cDNA and DNA polymerase GoTaq (Promega) according to the manufacturer’s instructions and consisted of 95 °C for 5 min and 15 cycles of 95 °C for 30 seconds ...
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bioRxiv - Bioengineering 2019Quote: ... a DNA fragment of 1191 bp was amplified using the pair of primers F_GFPseq / R_T0ter (Supplementary Table S6) with the GoTaq Flexi DNA polymerase (Promega) by colony PCR following manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... PCR was prepared using 5X GoTaq Green Reaction Buffer and GoTaq DNA Polymerase (Promega, Madison, WI, cat# M3005), 10 mM dNTPs ...
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bioRxiv - Synthetic Biology 2020Quote: ... A PCR reaction was performed with 0.5 U of GoTaq DNA Polymerase in GoTaq Buffer (both from Promega) supplemented with primers and dNTPs to a final volume of 20 µL ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNA samples prepared from E13.5-E14.5 whole embryos were used to amplify the target probes using GO Taq DNA polymerase (Promega). PCR products were cloned into pGEM-T Easy vector (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... zyxin(-/-) MEFs were transfected with 5 μg total DNA using the Fugene® 6 transfection reagent (E2691, Promega) and plated on glass coverslips.
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bioRxiv - Molecular Biology 2021Quote: ... Any residual genomic DNA was removed by treating RNA with RQ1 Rnase-free DNase (Promega, Madison, WI, USA) at 37°C for 30 min ...
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bioRxiv - Neuroscience 2020Quote: Genomic DNA was extracted from tail tissue using the Wizard SV genomic purification system (Promega Biotech, Beijing, China) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... PCR was performed in 15 µl reactions using 2x GoTaq DNA Polymerase master mix (Promega Cat. No. M3008) with 5 ng of gDNA and 500 nM of each primer ...
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bioRxiv - Cancer Biology 2019Quote: ... Complementary DNA (cDNA) was synthesized using M-MLV (Moloney murine leukemia virus) reverse transcriptase (Promega, Madison, WI, USA), and amplified using SYBR Green qRT-PCR SuperMix-UDG reagents (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... The DNA was extracted from the excised agarose using a PCR product purification kit (Promega, Madison, Wisconsin, USA), following the manufacturer’s instructions for agarose-embedded DNA ...
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bioRxiv - Microbiology 2019Quote: ... and the resulting 889 bp fragment was cloned in the pBB-MCS1 vector using T4 DNA Ligase (Promega). This plasmid MCS1_dam was then digested with AatII and SacI enzymes to obtain a DNA fragment of 2194 bp containing a chloramphenicol resistance gene and the dam gene controlled by the Plac promoter ...
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bioRxiv - Bioengineering 2020Quote: ... PCR reactions were carried out with 100 ng genomic DNA or cDNA in the GoTaq Master Mix (Promega) according to the manufacturer’s instruction ...
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bioRxiv - Genomics 2021Quote: ... T6 and T15 of each triplicate of each condition using the Wizard Genomic DNA Purification kit (Promega, A1125) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... were transcribed and translated using the TNT T7 Quick for PCR DNA Coupled Transcription/Translation System (Promega, USA). The reactions contained 10 µL of TNT ...
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bioRxiv - Cell Biology 2021Quote: ... 800 ng of total RNA were freed from genomic DNA using the RQ1 RNase-Free DNase Kit (Promega). Reverse transcription was performed using 145,4ng of the total DNase-treated RNA using the TaKaRa PrimeScript RT Master Mix Kit ...
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bioRxiv - Biochemistry 2021Quote: Cells were transfected for 48 h with 15 µg DNA per 15 cm dish using Fugene HD (Promega) following the manufacturer’s instructions using plasmids encoding full-length FLAG-tagged MeiP26 protein ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 μg of DNA-free RNA was then used for reverse transcription using M-MLV Reverse Transcriptase (Promega) in the presence of RiboLock ...
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bioRxiv - Developmental Biology 2021Quote: ... A 2kb DNA fragment upstream of TSS of Snail1 promoter (ENSGAL00000008018) was cloned into pGL3 basic plasmid (Promega) using PWO polymerase (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... 2mL of Sf9 cells at 0.5×106 cells/mL were transfected with 2μg of fresh bacmid DNA and FuGene HD transfection reagent (Promega) at a 3:1 transfection reagent to DNA ratio according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Positive PCR products were purified with the ReliaPrep™ DNA Clean-Up and Concentration System (Promega, Madison, WI). Genomic libraries were constructed with the Nextera XT DNA Sample Preparation kit (Illumina Inc. ...
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bioRxiv - Microbiology 2020Quote: ... and clones of the recombinant vector was obtained using Wizard® Plus SV Minipreps DNA Purification kit (Promega). The pACD4K-C vector contains a T7 promoter and ...
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bioRxiv - Microbiology 2021Quote: ... virus inoculum was removed and cells were then transfected with 2 µg of plasmid DNA using FuGeneHD (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2020Quote: 80,000 HEK293T cells were plated in 500μl HEK Media and 24 hrs later 500ng total plasmid DNA was transfected with 1.5μl FuGene HD (Promega) as follows ...
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bioRxiv - Genomics 2021Quote: ... HMW DNA was resuspended in 10 mM Tris pH 8 and quantified by Quantus™ Fluorometer (Promega Inc). The DNA size and integrity were assessed on TapeStation™ instrument (Agilent ...
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bioRxiv - Microbiology 2020Quote: ... gDNA extraction was carried out directly on the pellet cells with the Wizard Genomic DNA Purification Kit (Promega) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The resulting fragments were ligated to Nextflex adapters (Bio Scientific) with the use of T4 DNA ligase (Promega). The libraries were amplified using an AT-rich optimized KAPA protocol using KAPA HiFi HotStart ready mix (KAPA Biosystems) ...
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bioRxiv - Molecular Biology 2022Quote: ... Digested plasmids and gBlock DNA fragments were purified by Wizard SV Gel and PCR clean-up system (Promega) and transformed in Stbl3 competent cells ...
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bioRxiv - Immunology 2022Quote: ... MCMV-DNA was quantified by real-time PCR using BIO-RAD CFX with GoTaq qPCR Master Mix (Promega) and primers specific for MCMV glycoprotein B (gB ...