Labshake search
Citations for Promega :
2151 - 2200 of 2516 citations for DNA Sequencer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... The identity of all cell lines was confirmed by DNA finger printing analysis utilizing the GenePrint® 10 System (Promega). BT-474 and MDA-MB-453 cells were cultivated in DMEM medium (Pan-Biotech ...
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bioRxiv - Cancer Biology 2019Quote: ... Tumor tissue was manually microdissected from the slides and FFPE-DNA and RNA was isolated with the Maxwell system (Promega) according the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... Amplicons were purified using Qiagen PCR Cleanup (Cat no. 28104) and pooled with pTK nanotag reporter vector with T4 DNA ligase (Promega) and BsmBI (NEB ...
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bioRxiv - Microbiology 2021Quote: ... n=4 vaginal) and rUTI (n=3 urine) cohorts belonging to B2 phylogenetic group was extracted (Wizard Genomic DNA Purification Kit, PROMEGA) and sequenced by Illumina NovaSeq 2×150 nt ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Genomic DNA was extracted from a small piece of muscle tissue by using a Maxwell RSC Blood DNA Kit with a Maxwell RSC Instrument (Promega). A shotgun DNA library was constructed using a TruSeq Nano DNA High Throughput Library Prep Kit (Illumina) ...
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bioRxiv - Cell Biology 2020Quote: ... −2784 bp from the start codon to +688 bp from the stop codon) was amplified from Arabidopsis genomic DNA by PCR and integrated into pGEM-T Easy vector (Promega). First ...
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bioRxiv - Cell Biology 2019Quote: ... Hana 3A cells were authenticated using polymorphic short tandem repeat (STR) at the Duke DNA Analysis Facility using GenePrint 10 (Promega) and shown to share profiles with the reference (ATCC) ...
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bioRxiv - Biophysics 2021Quote: ... were plated into six-well tissue culture plates containing collagen-coated glass cover slips 12 to 24 h before being transfected with 1 μg of the indicated plasmid DNA using FuGENE 6 transfection reagent (Promega) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: ... Chromosomal DNA was extracted from cultures of cells at both mid-exponential growth phase and (non-replicating) stationary growth phase using the Wizard genomic DNA extraction kit (Promega), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... After 1 h incubation at 37°C the sample was cooled down and mixed with 30 Weiss Units of T4 DNA Ligase (Promega) and incubated for 4 h in a water bath at 16°C ...
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bioRxiv - Genomics 2021Quote: 293T cells were co-transfected with plasmid DNAs of a retroviral vector and the helper vectors using FuGENE HD (Promega). Medium was collected 24 ...
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bioRxiv - Microbiology 2020Quote: ... were then added to the 3’ ends of all fragments by incubation of 3 µg of size-selected DNA fragments with the recombinant terminal deoxynucleotidyl transferase (rTdT, 30 U/µL, Promega) at 37°C for 1H ...
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bioRxiv - Microbiology 2020Quote: ... glacialis A3 by filtering cells onto a 3-μm polycarbonate filter and using the Wizard SV Genomic DNA Purification kit (Promega) following the manufacturer’s instructions and quantified on a Qubit 3.0 fluorometer using the DNA high sensitivity assay kit (Thermo-Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... paraffin-embedded (FFPE) tissue slides using Maxwell® RSC Blood DNA Kit and Maxwell® RSC RNA FFPE Kit (Promega) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2021Quote: ... the sequences containing putative NRSE motifs were amplified by PCR from the human genomic DNA and then subcloned into the pGEM-T Easy vector (Promega). To generate plasmid templates for the mutated probes ...
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bioRxiv - Immunology 2021Quote: ... cells were transfected with a DNA mixture containing 50 ng of firefly luciferase reporter plasmid (Promega pGL4.31 [luc2P/Gal4UAS/Hygro]), 5 ng of Renilla luciferase plasmid (Promega pRL-CMV) ...
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bioRxiv - Microbiology 2021Quote: ... HEK293T cells were transiently transfected with plasmid DNA pHIV-1 NL4·3Δenv-Luc and Spike-Δ19-D614G by using Profection mammalian transfection kit (Promega Inc) following the instructions in the kit manual ...
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bioRxiv - Genetics 2020Quote: ... The DNA templates used for DIG-labeled STX18-AS1 RNA probe in vitro syntheses (Supplementary file) with T3 RNA polymerase (Promega) were amplified from the coding sequence of STX18-AS1 from human foetal hearts cDNA (obtained from HDBR) ...
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bioRxiv - Cell Biology 2020Quote: OCA2 enhancer region was cloned from mouse genomic DNA into KpnI and XhoI sites of the pGL3 promoter vector (E1751, Promega). MITF proximal promoter was cloned into SacI and HindIII sites of the PGL4 promoter vector (E6651 ...
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bioRxiv - Neuroscience 2022Quote: The scATAC-seq peaks that overlapped obesity-associated SNPs were PCR amplified from human genomic DNA (see primers in Supplementary Table 2) and cloned into the pGL4.23 plasmid (Promega, E84111). The associated SNPs were then introduced into these plasmids by PCR amplification with primers containing the associated variants ...
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bioRxiv - Genetics 2022Quote: ... The amplification was performed in 20 μL of reaction (2XPCR FailSafeTM PreMix E from Biosearch Technologies, 1.25 U of GoTaq DNA Polymerase (Promega), < 1μg DNA template and 0.5 μM of each specific primers ...
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bioRxiv - Cancer Biology 2022Quote: ... This was followed by a lysis step and DNA extraction was performed automatically with the Maxwell® RSC Instrument (Promega) by running the program Maxwell® RSC FFPE Plus DNA Kit (AS1720) ...
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bioRxiv - Cancer Biology 2022Quote: ... and a membrane GFP (75 ng as control for transfection) using FuGENE HD transfection reagent (1µg DNA/1µL FuGENE) (Promega, WI, USA). Next ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA was purified from the gel fragments using the Wizard SV Gel and PCR Clean Up kit (Promega, Fitchburg, USA) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2022Quote: ... Genome editing efficiency was checked by PCR on genomic DNA from the transfected population with the GoTaq G2 polymerase (Promega) using primers surrounding the 210 bp deleted region ...
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bioRxiv - Molecular Biology 2022Quote: ... sequence was amplified from human genomic DNAs and used as in vitro transcription template in the reaction using Riboprobe System-T7 Kit (P1440, Promega) in the presence of 0.4μL 5-(3-Aminoallyl)-uridine-5’-triphosphate labeled with ATTO 680(Aminoallyl-UTP-ATTO-680 ...
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bioRxiv - Genomics 2022Quote: ... A single colony was used to inoculate LB Broth and genomic DNA extracted from 1 mL using a Promega Wizard kit according to the manufacturer’s instructions (Promega, USA). DNA was quantified using the Qubit 3 and Nanodrop (Thermofisher ...
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bioRxiv - Molecular Biology 2022Quote: ... U2OS reporter cell lines were simultaneously co-transfected with siRNA using Dharmafect1 (Dharmacon) and DNA (RFP and I-Sce1 endonuclease expression constructs) using FuGene6 (Promega), respectively ...
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bioRxiv - Immunology 2022Quote: ... the indicated plasmids were added to a total of 2.0 ug DNA (with pLEX307 RFP as the filler plasmid) in 125 uL Opti-MEM and transfected using FuGENE HD (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... The primer and DNA marker were end-labeled with 10 μCi of [γ-32P]ATP by T4 polynucleotide kinase (Promega) and then used for reverse transcription with avian myeloblastosis virus (AMV ...
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bioRxiv - Microbiology 2022Quote: Salmonella strains were cultured overnight at 37°C in LB broth and DNA was extracted using a Maxwell RSC 48 instrument (Promega) and associated Maxwell RSC cultured cells DNA kit (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... Further taxonomic affiliation was carried out on these clones after PCR amplification of the rDNA genes using the same primers as above and Pfu DNA polymerase (Promega), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Hana 3A cells were authenticated using polymorphic short tandem repeat (STR) at the Duke DNA Analysis Facility using GenePrint 10 (Promega) and shown to share profiles with the reference (ATCC) ...
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bioRxiv - Molecular Biology 2023Quote: ... For ACE2 overexpression HEK293T cells were transfected for 24 h with human ACE2 (pCG1-hACE2, 200 ng DNA/well) using JetPrime (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR fragments were purified over gel and the DNA was recovered using the Wizard SV Gel and PCR Clean-Up System (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... RNA was transcribed from 1 ug of MIuI-linearized DNA using T7 RiboMAX Express Large Scale RNA Production System (Promega). Template DNA was degraded using RQ1 DNAse for 30 min at 4°C and RNA was purified using RNAeasy mini kit (Qiagen) ...
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bioRxiv - Microbiology 2023Quote: ... HEK293T cells were transiently transfected with plasmid DNA pHIV-1 NL4·3Δenv-Luc and Spike-Δ19-D614G by using profection mammalian transfection kit (Promega Inc.). After 48 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... The indicated DNA oligonucleotide and oligonucleotide ‘HDVrt’ were mutually extended upon one another using three thermocycles with GoTaq Hot-start DNA polymerase (Promega). This generated a double-stranded DNA comprising the DNA sequence of the desired RNA oligonucleotide ...
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bioRxiv - Genomics 2022Quote: ... DNA used for microarray was isolated from frozen cell pellets (3×106-7×106 cells) using the Maxwell RSC Cultured Cells DNA Kit on a Maxwell RSC 48 instrument (Promega). DNA was genotyped at the Children’s Hospital of Philadelphia’s Center for Applied Genomics using the Infinium Omni2.5-8 v1.3 BeadChip (Illumina ...
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bioRxiv - Neuroscience 2022Quote: ... Templates for RNA FISH probes were amplified by PCR from genomic DNA (Table S3) and cloned into pGEM-T Easy (Promega) or pCRII-TOPO ...
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bioRxiv - Molecular Biology 2022Quote: ... we amplified FES exons 9 to 11 with intervening sequences and SH3BP2 exons 9 to 11 with intervening sequences from human genomic DNA (Promega), and cloned the products into pcDNA3.1(+ ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR Tag analysis was performed either by adding 1.8 μL of yeast lysate used as template DNA to 6.25 μL of 2X GoTaq® Green Master Mix (Promega), 4.75 μL of nuclease-free water and 400 nM of each primer or by adding 1 µl of yeast lysate to 5 µl of DreamTaq Green PCR Master Mix (2X ...
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bioRxiv - Molecular Biology 2022Quote: The 3’ UTR of the human MAD2L1 with predicted miR-binding site was amplified from HCT116 genomic DNA and cloned to the psiCHEK2 located downstream of the Renilla translational stop codon into XhoI/NotI restriction enzymes (Promega). 3’UTR mutant was obtained by using the Site Directed Mutagenesis (New England Biolabs) ...
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bioRxiv - Zoology 2024Quote: ... The amplicons-containing were excised after gel-electrophoresis and extracted DNA by using Wizard SV Gel and PCR Clean-Up System (Promega). The amplicon DNAs were further analyzed for Sanger sequencing with the QuantumDye Terminator Cycle Sequencing Kit (Tomy Digital Biology ...
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bioRxiv - Microbiology 2024Quote: ... The quality of the DNA was estimated using Nanodrop and the concentration was measured using the QuantiFluor ONE dsDNA System (Promega).
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bioRxiv - Molecular Biology 2024Quote: ... Tail DNA from offspring was genotyped for the presence of the BAC transgene by analyzing 100 ng of tail DNA with the GoTaq PCR reaction kit (Promega) according to the manufacturer’s instruction by adding forward primer 5′-GAGGCTCGGGTGCGGCAGCTC −3′ and reverse primer 5′-GTGCAGTGTGAGCACCTGCTGTC-3′ for PCR with an initial denaturation for 3 min at 95 °C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Genomic DNA was isolated and purified using the Wizard Genomic DNA Purification Kit protocol for Gram negative bacteria at one-third volume (Promega), then quantified using SYBR Green I Nucleic Acid Stain (Invitrogen ...
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bioRxiv - Immunology 2023Quote: In situ nick-end labeling of nuclear DNA fragmentation was performed with a TUNEL apoptosis detection kit (DeadEnd Fluorometric TUNEL system, Promega) according to the supplier’s instructions.
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bioRxiv - Microbiology 2024Quote: Aliquots of the final populations were thawed and the gDNA was extracted using the Wizard Genomic DNA Purification Kit (Promega). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... Deletion mutants of Caskin2 and Caskin2-4A were generated by site-directed mutagenesis with the PCR-based overlap extension method using Pfu DNA polymerase (Promega), and fragments containing the different mutations were exchanged with corresponding fragments in the pUC19-CASKIN2-GFP plasmid ...