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Citations for Promega :
1851 - 1900 of 2370 citations for DNA Sequencer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Cells were transfected with 2 μg of FLAG-tagged β2 or β1 adrenergic receptor DNA using Fugene 6 (Promega) and seeded at a density of ∼70,000 cells.ml−1 on 18 mm glass coverslips 4-8 hours after transfection.
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bioRxiv - Cancer Biology 2019Quote: ... cDNA was stored at −20°C until analysed by polymerase chain reaction using GoTaq®G2 DNA polymerase (Promega) according to the manufacturers protocol ...
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bioRxiv - Genetics 2019Quote: ... we ligated amplified DNA from individuals homozygous for each allele into the multiple cloning site of the pGL4.23 (Promega) minimal promoter luciferase reporter vector in both the forward and reverse orientations with respect to the genome ...
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bioRxiv - Synthetic Biology 2020Quote: ... at 54°C for 10 min and the resulting cDNA amplified with GoTaq Flexi DNA polymerase (Promega; Madison, WI) for 8 PCR cycles in the same reaction with a final volume of 100 μL ...
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bioRxiv - Neuroscience 2021Quote: ... in which a 1309-bp genomic DNA fragment of the murine Gpr19 (−1083 to +226)-luciferase reporter (luc2P, Promega) was cloned into a vector engineered to contain the PB IRs and internal sequences necessary for efficient chromosomal integration (Nakanishi et al. ...
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bioRxiv - Zoology 2020Quote: ... Other sections were digested in a mixture of 97% Nuclei Lysis Solution (Promega; from the Wizard DNA isolation kit) and 3% 20mg/ml Proteinase K (Promega) ...
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bioRxiv - Molecular Biology 2020Quote: gDNA samples for library preparation were extracted from overnight LB cultures using the Wizard Genomic DNA Purification kit (Promega) as described above ...
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bioRxiv - Molecular Biology 2020Quote: ... and 0.5 ml (approximately 2 × 109 cells) were used for gDNA extraction with the Wizard Genomic DNA Purification kit (Promega), which typically yielded 50 μl of 0.5-1.5 μg/μl gDNA.
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bioRxiv - Microbiology 2020Quote: ... viral particles were purified by cesium chloride gradient centrifugation 82 and DNA extracted with Wizard Prep Columns (Promega, Corp.). Viral metagenome short-read libraries were prepared using the NexteraXT kit (Illumina ...
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bioRxiv - Neuroscience 2019Quote: ... and mouse Cbln2 E2 fragments were PCR-amplified from genomic DNA of individual species and inserted into pGL4.24 vector (Promega). The sequences of the PCR primers and synthetic oligonucleotides are listed in Supplementary Table 1.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The following amounts of DNA were used per well: 50 ng of pGloSensor™ −20F cAMP Plasmid (E1171, Promega), 125 ng of untagged Gαi1-WT ...
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bioRxiv - Cell Biology 2019Quote: ... cells were grown on Precision cover glass in 24-well plates or into glass bottom imaging dishes and transfected with 0.5 μg DNA construct complexed with Fugene6 (Promega) following the manufacturer’s instruction ...
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bioRxiv - Immunology 2019Quote: ... the indicated plasmids were mixed with an empty vector to a total of 2.0 μg DNA in 125 μl in Opti-MEM and transfected using FuGENE HD (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: ... genes were isolated from C57BL/6 mouse genomic DNA by standard PCR and inserted into the pGL4.10 vector (Promega).
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bioRxiv - Bioengineering 2019Quote: ... Ligations of the digested plasmid backbones and PCR products occurred overnight at 16°C using T4 DNA ligase (Promega) prior to transformation into chemically competent Novablue E ...
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bioRxiv - Genomics 2021Quote: ... DNA quantity and quality was verified with the Quantus fluorometer assay (QuantiFluor ONE dsDNA Dye, Promega, Madison, WI, USA) and Femto Pulse® Automated Pulsed Field (Agilent Technologies ...
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bioRxiv - Molecular Biology 2020Quote: Outward facing PCR reactions were performed on 6ng of random primed cDNA using the GoTaq G2 DNA polymerase (Promega) following the manufacturer’s recommendations in the Techne TC-3000 PCR thermal cycler (initial denaturation of 2 min 95 °C ...
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bioRxiv - Physiology 2020Quote: ... Blocked membranes were incubated for 2 hours with DNA probe synthesized using a Prime-a-Gene® kit (Promega) and washed in mild and stringent Southern wash buffers ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were harvested 48 hours post-transfection and genomic DNA was extracted using the Wizard® SV Kit (Promega). Genomic targeting efficiency for each gRNA was determined through annealing and digestion with T7 Endonuclease I (NEB ...
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bioRxiv - Microbiology 2021Quote: ... seriolae gDNA was extracted from 5-day old cultures using the Wizard® Genomic DNA Purification Kit (Promega, USA) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Confirmed heavy and light chain plasmid DNA were co-transfected in 293T cells (ATCC) using Fugene transfection reagent (Promega) in 24 well plates for preparing antibody supernatant for initial screening for their expression and antigen specificity as detailed in the following section ...
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bioRxiv - Bioengineering 2021Quote: ... on a 3.5 cm glass-based dish were transfected with 1.0 μg of EBFP (plasmid DNA) using 3 μL of FuGENE HD Transfection Reagent (Promega) in 10 μL of Opti-MEM (Life Technologies Corporation) ...
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bioRxiv - Pathology 2021Quote: ... The DNA concentration of each PCR product was determined using a QuantiFluor™-ST fluorescent quantitative system (Promega, USA), and mixed with the appropriate proportion based on sequencing requirements ...
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bioRxiv - Genomics 2020Quote: ... we cloned human DNA sequences (Coriell) containing the reference allele upstream of the minimal promoter in the luciferase reporter vector pGL4.23 (Promega) using the enzymes Sac I and Kpn I ...
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bioRxiv - Neuroscience 2021Quote: ... all DNA constructs were prepared from bacterial cultures grown at 37 °C using PureYield™ Plasmid Midiprep System (Promega) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... Marker genes in isolated DNA were PCR-amplified using GoTaq Master Mix (Cat. No. M5133, Promega, Madison, WI, USA) as described in Caporaso et al.[24] PCR products were cleaned and normalized using the SequalPrepTM Normalization Plate Kit (Cat ...
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bioRxiv - Microbiology 2021Quote: ... Translation of the DNA library was performed using T7 RiboMAX™ Express Large-Scale RNA Production System (Promega, USA), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: The total amount of enriched DNA released from the dMDA droplets was measured with Quantus™ Fluorometer (Promega Inc.) and fragment size distribution was inspected on TapeStation™ (Agilent ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from filter paper punches using the manufacturer’s protocol for Promega Maxwell DNA IQ Casework Sample kit (Promega) or Qiagen DNA Blood kit (Qiagen) ...
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bioRxiv - Neuroscience 2022Quote: ... the double stranded DNA HDR donor was prepared in a step-wise manner in pGEM-T Easy vector (Promega) by first cloning in approximately 800bp long homology arms that were amplified from C57BL6/N-derived genomic DNA (Left arm ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA from peripheral blood leukocytes was extracted using the Maxwell® RSC automatic extractor (Promega, Madison, Wisconsin, WI, USA), following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Semiquantitative PCR reactions were carried out with 1–2 μL of cDNA and 0.2 μL of GoTaq DNA Polymerase (Promega) in a total reaction volume of 30 μL according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Regions of interest were amplified in a PCR reaction with GoTaq G2 Flexi DNA polymerase (Promega, Madison, WI, USA), and PCR-amplified samples were run on a 1,2% TBE-agarose gel for size separation ...
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bioRxiv - Cancer Biology 2022Quote: ... a 3kb region of the promoter containing HIC1 binding sites was amplified by PCR from human genomic DNA and cloned into the pGl4.26-luc vector (Promega); primers are listed in supplementary Table S1 ...
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bioRxiv - Cell Biology 2022Quote: ... were transfected with 2 μg of fresh bacmid DNA and 6 μL of FuGene HD transfection reagent (Promega, E2311) according to the manufacture’s instruction ...
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bioRxiv - Cell Biology 2022Quote: ... and complementary DNA synthesis was performed using M-MLV reverse transcription in the presence of RNasin RNase inhibitor (Promega). PCR primers were all intron spanning ...
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bioRxiv - Genomics 2022Quote: ... Single nuclei were sorted into 384 well plates containing 1 µl M-Digestion Buffer containing proteinase K and ~0.05 pg Lambda DNA isolated from dcm+ E.Coli (Promega #D1501).
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bioRxiv - Genetics 2022Quote: ... The obtained DNA fragment containing the gtr1Q86L allele was cloned into the pGEM-T vector (Promega, Madison, WI, USA), verified by Sanger sequencing and used to co- transform protoplasts of the F ...
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bioRxiv - Synthetic Biology 2022Quote: ... The presence and orientation of DNA fragments were confirmed by colony PCR using GoTaq Green Master Mix (Promega, USA), whereas the sequence of the genetic construct was checked by Sanger sequencing reactions (Macrogen Inc. ...
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bioRxiv - Microbiology 2022Quote: ... [32P]-dCTP labelled hmbA and 18S rRNA gene-specific DNA molecules were used as gene probes using the random hexanucleotide-primer kit following the supplier’s instructions (Promega). As a loading control ...
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bioRxiv - Immunology 2022Quote: ... the indicated plasmids were mixed with an empty vector to a total of 2.0 µg DNA in 125 µL Opti-MEM and transfected using FuGENE HD (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... the indicated plasmids were mixed with an empty vector to a total of 2.0 µg DNA in 125 µL Opti-MEM and transfected using FuGENE HD (Promega) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2022Quote: Complementary DNA was prepared from total RNA extracted from indicated samples using the ImProm-II Reverse Transcription System (Promega). qRT-PCR analysis was performed using a master mix with ROX reference dye (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... were transfected with 320 ng 12x MS2-tagged repeat expression construct and 80 ng CMV-BFP plasmid DNA (for labeling transfected cells) mixed with 1.6 μl FuGENE HD (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 1 mL of cells was then processed using the Promega Wizard® genomic DNA preparation kit (Promega, WI, USA) to obtain genomic DNA ...
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Throat colour polymorphism in relation to sex and body size of the Litter skink, Lankascincus fallaxbioRxiv - Zoology 2022Quote: ... black and white throat colored individuals using Promega Wizard® genomic DNA purification kit (Promega Corporation, Madison, Wisconsin, USA) following manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were cultured for 24 h with a complex of DNA and FuGENE® HD Transfection Reagent (#E2311; Promega) at a ratio of 1:3 prepared in Opti-MEM reduced serum medium (#31985070 ...
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bioRxiv - Microbiology 2023Quote: ... lactis CICC 6242 strain producing nisin A was extracted using a “Wizard Genomic DNA Purification” kit (Promega, Madison, USA). The nisA and nisRK genes were amplified through a polymerase chain reaction using the primers listed in Table 1 [29] ...
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bioRxiv - Genomics 2022Quote: ... Quantification of DNA was measured using fluorescence intensity of Quantifluor One dsDNA dye (Promega Quantifluor One dsDNA dye; E4871), inside a 0.5 milliliter thin-walled PCR tube ...
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bioRxiv - Microbiology 2023Quote: ... and plasmid extractions were done using the Wizard Plus SV Minipreps DNA Purification System according to the manufacturer’s protocol (Promega). The pk18mobsacB vector contains a kanamycin resistance cassette and the sacB gene ...