Labshake search
Citations for Promega :
2051 - 2100 of 2516 citations for DNA Sequencer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... PCRs were used to assay for wild-type or T-DNA insertion alleles in 20 μL reactions using GoTaq Green Master Mix (Promega) using manufacturer’s instructions and 30 cycles as follows ...
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bioRxiv - Biochemistry 2021Quote: ... Genomic DNA was eliminated by incubating 10 μL of isolated RNA with 1 U of RQ1 RNase-free DNase I (Promega) for 30 min at 30°C ...
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bioRxiv - Biochemistry 2021Quote: ... Genomic DNA was eliminated by incubating 2 μg of RNA with 2 U of RQ1 RNase-free DNase I (Promega) for 30 min at 30°C ...
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bioRxiv - Microbiology 2021Quote: ... The resulting libraries were checked for their quality using the High-sensitivity DNA chip using the Agilent 2100 Bioanalyzer (Waldbroon, Germany) and quantified using the QuantiFluor One dsDNA kit (Promega). Paired-end (2x300bp ...
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bioRxiv - Microbiology 2021Quote: Promoter fusions to lacZ were constructed by amplification of promoter regions from genomic DNA with the specified primers (Table S4) and ligated into pGEM-T Easy (Promega) for amplification and collection ...
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bioRxiv - Genetics 2021Quote: ... a 599 bp long construct was PCR amplified from genomic DNA isolated from the tail of a Pahenu2 homozygous animal using GoTaq G2 HotStart Green Master Mix (Promega). Primers are listed in Suppl ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and from the interior tissue (at least 2 mm away from the outermost edge of the specimen) using the Wizard Genomic DNA Purification Kit (Promega), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... DNA fragments of approximately the correct size were extracted from 1% agarose gels and ligated into the pGEMT-Easy (Promega) subcloning vector ...
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bioRxiv - Genomics 2020Quote: DNA was extracted from the fin clips of the challenged fish using a commercial kit (Wizard Genomic DNA Purification Kit, Promega), following the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... instrument was configured with the Standard Elution Volume (SEV) hardware and using the Maxwell 16 Cell DNA purification kit (Promega). For total RNA isolation ...
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bioRxiv - Neuroscience 2021Quote: Radioactive U1 and U7 snRNAs were generated by run-off transcription with T7 polymerase from linearized plasmid DNA templates in the presence of m7G cap analog (Promega) using the MEGAshortscript T7 kit (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: Preserved tissue (10-15 mg) was used for nucleic acid purification by employing the Wizard genomic DNA kit (Promega, USA) and following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: DNAseI treated total RNA from mESCs was used to synthesize complementary DNA (cDNA) using random primers (Promega Cat no. C1181), dNTP mix (LAROVA Cat no ...
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bioRxiv - Molecular Biology 2020Quote: ... ATFS-11-100(R/R)::GFP and Su91-69::GFP) were synthesized in reticulocyte lysate in the presence of [35S]methionine (T7 Quick for PCR DNA, Promega). Import into isolated mitochondria was performed in import buffer (3 % (w/v ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmids were eluted with RNase free water and used as templates for in vitro gene expression (E. coli S30 Extract System for Circular DNA, Promega).
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bioRxiv - Molecular Biology 2019Quote: ... of linearized DNA were used as templates for in vitro transcription in the T7 RiboMAX Large Scale RNA Production System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA was extracted from selected clones as well as in the parental clone using the Wizard Genomic DNA Purification Kit (Promega). NGS was performed using Nextera XT DNA Library Preparation kit and the NextSeq 500 sequencing systems (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... The GAL1 promoter was amplified from yeast genomic DNA using primers 4588-1/XH5103 and ligated into pGEM-Teasy (Promega) to create pCB4554 ...
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bioRxiv - Cell Biology 2019Quote: ... Transfections were performed with 4 μg of DNA, 800 μL of OptiMEM (Invitrogen, and 12 μL of FuGENE 6 transfection reagent (Promega) per 10 cm dish ...
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bioRxiv - Genomics 2019Quote: All plasmids used in this study were prepared from cultures grown overnight in LB using the Wizard Plus SV Minipreps DNA Purification System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... Inverse PCR reactions were carried out with 2 μL of DNA solution in a final volume of 20 μL using the GoTaq enzyme (Promega). The PCR conditions were as follows ...
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bioRxiv - Microbiology 2019Quote: ... The genes of interest along with ∼700 bp of 3’ and 5’ flanking DNA were amplified and cloned into the vector pGEM-T Easy (Promega) to create pGEMlpg0277 ...
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bioRxiv - Microbiology 2019Quote: ... All DNA extractions were done with the Wizard® SV Gel and PCR Clean Up system kit (Promega, Madison, USA).
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bioRxiv - Evolutionary Biology 2019Quote: DNA was extracted from stickleback fin clips using the Wizard® SV Genomic Purification kit (Promega® Corp., Madison, USA) and quantified using Picogreen® ds DNA assay (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... A cDNA encoding Myc epitope-tagged human TRAPα was synthesized by Integrated DNA Technologies (IDT) and was subcloned into the pTarget mammalian expressing vector (Promega).
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bioRxiv - Evolutionary Biology 2019Quote: ... DNA quality and quantity were assessed using 1% agarose gel electrophoresis and QuantiFluor dsDNA System in a Quantus fluorometer (Promega). NextRAD libraries were constructed by SNPsaurus (SNPsaurus ...
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bioRxiv - Microbiology 2019Quote: DNA from fecal samples and a blank control were isolated with the use of the Maxwell 16 Instrument (Promega Corporation) following the manufacturer’s instructions for the low elution volume blood DNA system ...
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bioRxiv - Microbiology 2019Quote: ... Lifted cells were resuspended with an additional 50 µl of DMEM before adding to 50 µl Nuclei Lysis Buffer (Wizard Genomic DNA Purification kit; Promega) and frozen at −20°C overnight ...
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bioRxiv - Genomics 2019Quote: ... Tagmentation was performed on 250,000 HCT116 Parental and DKO cells using 700nM Tn5 Transposase assembled using preannealed Tn5ME-A_mC and Tn5ME-B_mC (Table S3) for 30 minutes at 37°C following the addition of 0.01ng of unmethylated Lambda DNA (Promega #D1521). We recommend performing a titration of Tn5 transposase to nuclei input to assay minimum amounts required as in Figure S5 ...
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bioRxiv - Plant Biology 2019Quote: ... DNA quality and quantity were assessed using 1% agarose electrophoresis and the QuantiFluor dsDNA System with a Quantus fluorometer (Promega). Nextera-tagmented reductively-amplified DNA (nextRAD ...
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bioRxiv - Plant Biology 2019Quote: ... 5 μL of 5× Green GoTaq® Flexi Buffer and 0.25 μL of 5U GoTaq® DNA Polymerase (Promega, USA) in a total volume of 25 μL ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and from the interior of the sponge tissue (<2mm from the exterior pinacoderm) and the seawater filter papers using the Wizard Genomic DNA Purification Kit (Promega).
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bioRxiv - Biochemistry 2020Quote: ... Bacmid DNA was prepared by isopropanol precipitation and transfected into Sf9 (Spodoptera frugiperda) insect cells using FuGENE HD transfection reagent (Promega). After two rounds of virus amplification in Sf9 cells ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were genotyped by extracting DNA from tail clippings with Extracta DNA Prep for PCR – Tissue (Quanta Biosciences) and specified products amplified using either GoTaq Green Mastermix (Promega) or 2x KAPA buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Genomic regions flanking the CRISPR target sites were PCR amplified with designed primers (Supplemental Table 3) using GoTaq DNA polymerase (Promega) and sent for Sanger sequencing to determine the insertion and deletion errors generated by CRISPR-Cas9 system in exon 2 of PARKIN ...
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bioRxiv - Bioengineering 2021Quote: ... Ligations of the digested plasmid backbones and PCR products occurred for 5-10 minutes at RT using T4 DNA ligase (Promega) prior to transformation into NEB® Turbo Competent E ...
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bioRxiv - Biochemistry 2021Quote: ... FN1-GFP together with pRSV40 Renilla (2% of total transfected DNA) were transiently transfected and lysed in Passive Lysis Buffer (Promega). Cell lysates were analyzed by Western blot while Renilla luciferase activity (Glomax luminometer ...
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bioRxiv - Neuroscience 2019Quote: ... in wells of a 6-well plate and transfected 24 hours later with 500-800 ng plasmid DNA using FUGENE 6 (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: Genomic DNA was extracted using HotSHOT (hot sodium hydroxide and tris) and PCR was performed using GoTaq green master mix (Promega) (Truett et al ...
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bioRxiv - Cell Biology 2021Quote: ... was prepared from tachyzoites of RH or RH ΔKu80 (here referred as Δku80) strains using the Wizard SV genomic DNA purification (Promega) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: A DNA fragment bracketing the proximal promoter and transcription start site of the Iqsec3 gene (–280 to +36) was PCR-amplified from mouse genomic DNA and subcloned into the pGL3-Basic vector (Promega). Full-length mouse Npas4 cDNA was subcloned in pcDNA3 (Gibco-Invitrogen) ...
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bioRxiv - Plant Biology 2021Quote: ... Halo fusion proteins were produced from 1.5 µg plasmid DNA in a 15 µL reaction using TNT SP6 High-Yield Wheat Germ Protein Expression System (Promega). Protein expression was confirmed by Western blot with Anti-HaloTag monoclonal Ab (1:2000 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Plasmids were resuspended in RNase-free water and used as templates for in vitro gene expression (E. coli S30 Extract System for Circular DNA, Promega). Reactions were set up by adding 1 μL of 0.25 mM amino acid mix ...
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bioRxiv - Plant Biology 2020Quote: ... 10 μL DNase I digested small RNA extracted from NF- and NF+ were reverse transcribed with random primers and the cDNA was used for PCR by using GoTaq flexi DNA polymerase (Promega). 100 ng total RNA extracted from Arabidopsis protoplast cells were reverse transcribed and used as positive control to indicate the proper size of amplified products ...
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bioRxiv - Microbiology 2020Quote: ... and digested for 2 hours at 37°C with ApaI and SpeI restriction enzymes and ligated with T4 DNA ligase (Promega) overnight at 16° to a double-digested pKNG101 plasmid ...
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bioRxiv - Microbiology 2021Quote: High molecular weight DNA was extracted using the Maxwell® RSC PureFood GMO and Authentication Kit as for qPCR (Promega). The high molecular weight DNA was sent to the Functional Genomic Center Zurich (FGCZ ...
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bioRxiv - Microbiology 2021Quote: ... Tubulin β (ML841/ML842) or actin (ML843/ML844) were used to amplify specific transcripts with the GoTaq DNA polymerase (Promega). PCR was performed with 21 cycles of denaturation (30 s ...
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bioRxiv - Microbiology 2020Quote: gDNA was extracted from stationary phase evolved cocultures following revival from frozen stocks using a Wizard Genomic DNA purification Kit (Promega). DNA fragment libraries were constructed for samples from shaking WT-based cocultures A-F and NifA*-based cocultures M-R at generation ∼146 using NextFlex Bioo Rapid DNA kit ...
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bioRxiv - Microbiology 2019Quote: ... Transient transfections were carried out for 16-20h with either 500 ng of construct DNA + 500 ng of pSFFV as filler using FugeneHD (Promega) or 1 µg of construct DNA using Polyjet transfection reagent (Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The PCR reaction was carried out in a 50 μl reaction volume containing 1.25 U GoTaq Flexi DNA Polymerase (Promega, USA), 10 μl of 5X Flexi Reaction Buffer (Promega ...