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Citations for Promega :
1701 - 1750 of 2516 citations for DNA Sequencer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... was digested with the corresponding enzymes and ligated with amplified genes using T4 DNA ligase (Promega).
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bioRxiv - Genetics 2022Quote: ... the genomic DNA was used as a template for the PCR reactions using Go-Taq (Promega). The cycling consisted in initial denaturation ...
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bioRxiv - Microbiology 2022Quote: ... 1 μL of ligation product was amplified using PCR with a GO Taq DNA polymerase (Promega) and Illumina primers (P7 5’-CAAGCAGAAGACGGCATACGAGATAGACCGGGGACTTATCATCCAACCTGT-3’ ...
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bioRxiv - Microbiology 2022Quote: ... Positive cells were multiplied and their plasmid extracted using the Wizard Minipreps DNA purification system (Promega). Plasmids were electroporated into WT B ...
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bioRxiv - Immunology 2022Quote: ... 0.5 μl 10 μM Reverse primer and 0.125 U/μl GoTaq DNA Polymerase (Promega, Madison, WI) were mixed to make a 25 μl PCR reaction mix (key resource table) ...
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bioRxiv - Biochemistry 2022Quote: ... Product length was determined with respect to ladder product sizes of Lambda x HindIII DNA (Promega). The front (fastest migrating population ...
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bioRxiv - Plant Biology 2022Quote: ... Diagnostic PCR was performed using GoTaq G2 Flexi DNA polymerase according to a manufacturer’s protocol (Promega). Primers were designed using Primer3 0.4.0 software and synthesized by Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Plasmids were purified from these cultures using a Wizard Plus SV Miniprep DNA Purification kit (Promega). All inserted sequences were confirmed by Sanger sequencing using M13 forward and reverse primers (M13F and M13R ...
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bioRxiv - Microbiology 2022Quote: Viral genomes or gDNA from infected amoebas were purified using Wizard genomic DNA purification kit (PROMEGA). To determine the amplification kinetic ...
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bioRxiv - Plant Biology 2023Quote: ... DNA fragments were excised from the gel by means of x-tracta™ Gel Extractor (Promega) and purified using Wizard® SV Gel and PCR Clean-Up System (Promega) ...
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bioRxiv - Neuroscience 2024Quote: Complementary DNA (cDNA) was synthesized from RNA using the ImProm-II Reverse Transcription System (Promega, #A3800) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 1/20 of the cDNA was amplified by PCR using the GoTaq DNA polymerase mix (Promega) with specific antigenome primers (Appendix Table S1) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cells were transfected with 1 or 1.5 μg plasmid DNA using FuGene HD (Promega, #E2311) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... was used to transcribe luciferase mRNA from the linear luciferase DNA template (1,800 bases, Promega, UK). Generally ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified BAC and plasmid DNA were transfected into HEK293 cells with FuGene HD transfection reagent (Promega) in a 1:3 DNA:FuGene HD ratio ...
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bioRxiv - Biochemistry 2023Quote: ... Purified DNA was ligated into a pGEM-T Easy pre-linearized vector (Promega, catalog no. A1360) and transformed into DH5α competent cells ...
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bioRxiv - Biochemistry 2023Quote: ... site directed mutagenesis was performed using Pfu DNA polymerase as per manufacturer’s instructions (Promega, Cat. # M7741). The resulting sequence verified plasmids having the desired mutation were individually transformed into E ...
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bioRxiv - Neuroscience 2023Quote: Complementary DNA (cDNA) was synthesized from RNA using the ImProm-II Reverse Transcription System (Promega, #A3800) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were transfected with 500 ng DNA at approximately 70% confluence using ViaFect transfection reagent (Promega) and assayed 24 hours after transfection ...
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bioRxiv - Microbiology 2023Quote: DNA extraction was performed using a magnetic bead-based protocol on an automated Maxwell machine (Promega). Whole-genome sequencing of 62 (of total 66 ...
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bioRxiv - Plant Biology 2023Quote: ... Genomic DNA was then digested out using the RQ1 RNase-Free DNase (Cat. No. M6101; Promega). cDNAs were obtained from 400 ng of RQ1-treated RNA using 1 μl of GoScript Reverse Transcriptase (Cat ...
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bioRxiv - Molecular Biology 2023Quote: ... Both DNA and RNA luminescence assays were performed using the Dual-Glo Luciferase assay system (Promega) following the manufacturer’s instructions ...
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DNA Recognition and Induced Genome Modification by a Hydroxymethyl-γ Tail-Clamp Peptide Nucleic AcidbioRxiv - Genetics 2023Quote: ... genomic DNA (gDNA) was harvested using the Promega ReliaPrepTM gDNA Tissue Miniprep System (Promega, Madison, WI). Similarly ...
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bioRxiv - Molecular Biology 2022Quote: ... Insertion of nucleotides into expression vectors was done with T4-DNA ligase (#M1804, Promega, Madison, USA) via EcoRI/NotI (#R6017/#R6435 ...
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bioRxiv - Cell Biology 2023Quote: ... then transfected with 4 μg pLZRS-GCaMP6 DNA plus 12 μl FuGENE 6 (Promega Cat. #E2691) in 800 μl of Opti-MEM (Thermo-Fisher Cat ...
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bioRxiv - Genomics 2023Quote: ... The single-stranded DNA generated using this method was purified using PCR purification kit (A9285; Promega) and quantified using Nanodrop ...
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bioRxiv - Cell Biology 2023Quote: HeLa cells were transfected using 2 µg of plasmid DNA and 6 µL of FuGENE (Promega) for gene editing or using a NEPA21 electroporation system (Nepa Gene ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Purification was performed to remove adaptors and primer dimers using the Wizard Genomic DNA Kit (Promega) and quality was verified by measuring the quantity and length of the fragments ...
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bioRxiv - Neuroscience 2023Quote: ... Mutations additionally identified with an AAF suggestive of a germline event in control DNA samples (Promega genomic control and unrelated individual’s brain tissue) ...
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bioRxiv - Neuroscience 2023Quote: ... 1.25µl was used for the PCR reaction employing GoTaq G2 DNA Polymerase (M7845, Promega, Dübendorf, Switzerland) with primers (Microsynth ...
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bioRxiv - Immunology 2023Quote: ... The linearized plasmid was then purified using the Wizard Plus SV Miniprep DNA Purification System (Promega), and saRNA was transcribed in vitro using HiScribe T7 High Yield RNA Synthesis Kit (NEB) ...
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bioRxiv - Biochemistry 2023Quote: ... 0.02 µg of plasmid and 2 µg of carrier DNA were combined with FuGENE HD (Promega) at a 3:1 ratio and added per well of a 6-well plate seeded with 70% confluent HEK293 cells ...
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bioRxiv - Genomics 2024Quote: ... and plasmids were purified using a plasmid DNA miniprep kit according to manufacturer’s instructions (Promega A1460). For synthesis of sense and antisense riboprobes ...
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bioRxiv - Microbiology 2023Quote: Swabs designated for microbial culture were spun down using DNA IQ Spin Baskets (Promega, Madison, WI). A portion of each sample was serially diluted with 1X phosphate buffered saline and plated onto Tryptic Soy Agar (TSA ...
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bioRxiv - Molecular Biology 2023Quote: ... Both PCRs were performed by using the GoTaq® Master Mix DNA polymerase (Promega, Madison, WI), 10 ng of gDNA and in a 10 µl reaction volume ...
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bioRxiv - Physiology 2023Quote: ... The gDNA was isolated from cell pellets using the Wizard Genomic DNA Purification kit (Promega, A1120). Preparation and Illumina short read sequencing (PE 150 ...
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bioRxiv - Genomics 2024Quote: ... (i) quantification of DNA samples of multiple sexed green toads (QuantiFluor® dsDNA System kit, Promega), (ii ...
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bioRxiv - Microbiology 2023Quote: ... Complimentary DNA was prepared from 1 µg of RNA using a Reverse Transcription Systems Kit (Promega). Quantitative PCR (qPCR ...
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bioRxiv - Synthetic Biology 2024Quote: ... Successful DNA assembly was verified first by colony PCR using GoTaq® Green Master Mix (Promega) and then with double restriction digestion with EcoRI-HF (NEB ...
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bioRxiv - Physiology 2024Quote: ... Plasmid and genomic DNA (gDNA miniprep kits were purchased from Promega. E. coli NEB 5α (NEB) was used as a host for plasmids ...
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bioRxiv - Developmental Biology 2024Quote: ... and then reverse-transcribed into complementary DNA (cDNA) with the GoScript Reverse Transcription System (Promega, A5003). cDNA was used as the template for the quantitative PCR assay using 2×SYBR Green qPCR Master Mix (Bimake ...
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bioRxiv - Genetics 2024Quote: ... The reaction was performed in a 50-μL mixture containing 1.25 U GoTaq DNA polymerase (Promega), 200 μM dNTPs ...
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bioRxiv - Plant Biology 2020Quote: ... and ligated with XbaI- and XhoI-digested pYL156 (Liu et al., 2002) using T4 DNA ligase (Promega). Ligations were transformed into house-made chemically competent E .coli DH10B ...
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bioRxiv - Cell Biology 2020Quote: ... genomic DNA was sheered and subjected to bisulfite conversion using the MethylEdge Bisulfite Conversion kit (Promega, N1301) using a bead-based automated protocol ...
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bioRxiv - Microbiology 2021Quote: ... and a 348-bp FoMV CP fragment was amplified using GoTaq DNA polymerase (Promega, Madison, WI, USA). The cDNA was diluted 40 times and then 2 ul were used for the RT-qPCR ...
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bioRxiv - Genomics 2022Quote: ... coli isolates from Vietnam was extracted using the Wizard Genomic DNA purification kit (Promega, Madison, WI, USA) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... All nucleotide sequences were amplified by PCR from PXO99A genomic DNA and cloned into pGEM-T (Promega). These intermediate constructs were verified by sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... For PCR genotyping with primers listed in Supplementary Table S5 and the GoTaq G2 DNA Polymerase (Promega) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... DNA plasmids or dsRNAs were transfected into the cells using FuGene HD transfection reagent (Promega, Cat #PRE2311) according to the manufacturer’s protocol.
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bioRxiv - Biochemistry 2020Quote: ... The reverse transcriptase M-MLV RT as well as the T4 DNA ligase were obtained from Promega. The pGEM-T Vector kit was purchased from Promega ...