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Citations for Promega :
1801 - 1850 of 2370 citations for DNA Sequencer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... All PCR reactions were run using 2 µL of extracted DNA in GoTaq® PCR Master Mix (Promega) and the following cycling protocol ...
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bioRxiv - Microbiology 2023Quote: Transfection of DNA plasmids into different mammalian cells was conducted using FuGENE® HD (Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with plasmid DNA by the calcium phosphate or lipofection method using FuGene 6 (Promega, WI) (Yoshida et al. ...
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bioRxiv - Developmental Biology 2023Quote: KO pool cells were collected and gDNA was extracted using Wizard® Genomic DNA Purification Kit (#A1120, Promega) following the manufacturer’s instruction ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The following amounts of DNA were used per well: 60 ng of pGloSensor-22F cAMP plasmid (Promega, E2301), 100 ng of receptor or empty vector (control ...
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MET functions in tumour progression and therapy resistance are repressed by intronic polyadenylationbioRxiv - Molecular Biology 2023Quote: ... PCR reactions were performed using 10 ng of cDNAs (RNA equivalent) and the GoTaq DNA polymerase (Promega, # M8295). For each gene ...
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bioRxiv - Synthetic Biology 2023Quote: ... strain ADP were extracted using Wizard™ Genomic DNA Purification Kits following the standard protocol provided by Promega.
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bioRxiv - Genomics 2023Quote: ... DNA was isolated using an automated magnetic bead purification method (Hamilton Microlab STAR liquid handling machine and Promega ReliaPrep Large Volume HT gDNA isolation system ...
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bioRxiv - Microbiology 2023Quote: ... The size of any positive IS2404 detection was assessed against 2 µL of 100 bp DNA Ladder (Promega) and run at 50 V for 1.5 hrs before being visualised with an EZ Gel Documentation System (Bio-Rad) ...
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bioRxiv - Genomics 2023Quote: ... Transfection of plasmid DNA (2.5μg vector + 1μg of transposase plasmid) was performed using the FuGENE transfection reagent (Promega #E5911) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA integrity was assessed by electrophoresis and genomic DNA was eliminated with RQ1 DNAse (Promega, Madison, WI, USA) and subsequent chloroform extraction and ethanol precipitation ...
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bioRxiv - Plant Biology 2024Quote: ... 1 µL of extract was used as a DNA template for 1× Gotaq® Green Master Mix (Promega) 25 μL volume PCRs ...
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bioRxiv - Molecular Biology 2023Quote: ... Ligation of the digested pGEM-T easy vector and 5’ fragments was carried out overnight at 4 °C using 0.5 µl (1.5 U) of T4 DNA Ligase (Promega). pGEM-T easy vector containing the 5’ fragment (5’-pGEM-T ...
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bioRxiv - Genetics 2024Quote: 2000ng DNA (fixed or non-fixed) was mixed with 10μL Brilliant III SYBR Green qPCR Master Mix (Promega) and the DNA melting behaviour was analysed in the CFX96 Touch Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: Correct integration of the homology template at the desired location was assessed by PCR (GoTaq DNA Polymerase, Promega) on extracted genomic DNA (Promega Wizard DNA Extraction ...
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bioRxiv - Neuroscience 2024Quote: ... Specific amplicons were prepared using several primer pairs (Table 1) in combination with GoTaq© DNA Polymerase (Promega).
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bioRxiv - Molecular Biology 2024Quote: ... each WT ZNF410 peak was amplified by PCR from genomic DNA and cloned into pGL4.24 vector (Promega, #E8421), digested by KpnI and XhoI ...
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bioRxiv - Cancer Biology 2024Quote: ... The cell identity was confirmed through K562 STR DNA fingerprinting (https://www.atcc.org/products/ccl-243) by GenePrint 10 System Kit (Promega).
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bioRxiv - Cancer Biology 2023Quote: ... according to the manufacturer’s instructions and PCR amplification was performed using the GoTaq Flexi DNA polymerase kit (Promega). Organoid genotype was confirmed by Sanger sequencing ...
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bioRxiv - Cancer Biology 2021Quote: ... The digested vector (200 ng) was ligated with 1 µL of the oligonucleotides (0.45nmol/μl) using T4 DNA ligase (Promega) for 3 hrs ...
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bioRxiv - Microbiology 2020Quote: ... Cytosine tails (C-tails) were added to 1 µg sheared DNA using terminal deoxynucleotidyl transferase (TdT, Promega, Madison, WI). Transposon containing fragments were amplified in a PCR containing DNA from the TdT reaction as template and primers specific to the ColE1 site on the 5’ end of the transposon ...
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bioRxiv - Microbiology 2019Quote: ... 500 nM of each primer (5’-TCCTGCTCAACTTCCTGTCGAG-3’ and 5’-CACAGGTCAAACCTCCTAGGAATG-3’) and 0.1 µL hot-start Taq DNA polymerase (Promega) in 20 mM Tris−HCl pH 8.3 ...
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bioRxiv - Microbiology 2022Quote: ... DNA from the saliva samples (n=67, one missing) was extracted using the ReliaPrep Blood gDNA Miniprep (Promega, USA). For microbiome profiling ...
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bioRxiv - Molecular Biology 2019Quote: ... Complementary DNA (cDNA) was synthesized from 1 μg of total RNA using random primers and Reverse Transcriptase System (Promega). The cDNA was used as a template for the real-time PCR amplification using PowerUp SYBR® Green Master Mix and a StepOne Plus system (Applied Biosystem ...
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bioRxiv - Cell Biology 2019Quote: ... was amplified from mouse genomic DNA and cloned upstream of luciferase cassette in KpnI/HindIII site of pGL4.23 (Promega) using primers listed in Supplementary table 1 ...
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bioRxiv - Biochemistry 2019Quote: ... Mutations were verified by sequencing (Macrogen) and DNA for transfection was purified using the PureYield Plasmid MaxiPrep System (Promega).
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bioRxiv - Neuroscience 2019Quote: ... Amplified products were visualized with reference to DNA ladders (either: Hyperladder I, Bioline, London, UK, or 1kb ladder, Promega), using GeneSnap (Syngene ...
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bioRxiv - Molecular Biology 2019Quote: ... were plated in T25 flasks and transiently transfected with 2.5 µg plasmid DNA per flask using FuGENE HD transfection reagent (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... DNA-free RNA (500 µg) was used for reverse transcription using the Moloney Murine Leukemia Virus Reverse transcriptase (Promega, Madison ...
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bioRxiv - Synthetic Biology 2019Quote: Two to three colonies of TOP10 transformed cells were selected to verify through PCR with GoTaq DNA Polymerase (Promega) if the plasmids they contained were the desired constructions ...
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bioRxiv - Bioengineering 2019Quote: ... Linearized plasmid DNA was purified using the Wizard® SV Gel and PCR Clean-up Kit (Promega, Madison, WI) in preparation for transfection into Jurkat cells.
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Low basal expression and slow induction of IFITM3 puts immune cells at risk of influenza A infectionbioRxiv - Immunology 2019Quote: HEK293 IFITM3−/− cells were plated in a 6-well dish for 24 hours prior to transfection of IFITM3-pcDNA3.1 plasmid DNA using FuGene 6 reagent (Promega) at a ratio of 3:1 ...
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bioRxiv - Microbiology 2021Quote: ... 5 μL of DNA or 5 μL of cell lysate were mixed with 12.5 μL of GoTaq Colorless Master Mix (Promega), 1 μL of forward and reverse primer (5 μM ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from high titer lysates using the Wizard® Clean-Up Kit (cat. # A7280, Promega, WI, USA). Sequencing libraries were constructed with the NEBNext® UltraTM II DNA Library Prep kit (New England Biolabs ...
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bioRxiv - Bioengineering 2020Quote: ... The screened colony was later cultivated in YPD media and genomic DNA was extracted with Wizard genomic kits (Promega). Then the genomic samples were used as template for PCR verification of the integrated gene with gene-specific primers.
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bioRxiv - Cancer Biology 2020Quote: ... and DNA was digested using RQ1 RNase-Free DNase kit according to manufacturer’s instructions (Promega, Madison, WI, Cat#: M6101). Finally ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from cultures using the ReliaPrep gDNA tissue Miniprep system following the manufacturer’s guidelines (Promega, USA). This DNA was screened for Brucella DNA using Brucella specific primers (Table 1 ...
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bioRxiv - Plant Biology 2021Quote: ... This amplicon was introduced into pSKX1_talFMAI1 between the PvuI and HindIII restriction sites using T4 DNA ligase (Promega®) according to manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2020Quote: ... Absolute quantification was obtained with DNA fragment from the Map gene cloned in pGEM®-T Easy vector (Promega) with 10 to 108 copies of the plasmid added on each PCR plate ...
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bioRxiv - Microbiology 2020Quote: ... The radioactive DNA probes used were prepared using Prime-a-Gene Labeling System kit (U1100, Promega, Madison, WI, USA), and radiolabeled dCTP (PerkinElmer ...
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bioRxiv - Cancer Biology 2020Quote: ... Presence or absence of transcript was detected by a regular PCR using GoTaq® G2 DNA Polymerase (Promega, M7841) from 100ng cDNA and visualized on an agarose gel.
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bioRxiv - Cell Biology 2021Quote: RNA extraction from FFPE specimens of chRCC was performed using the Maxwell® 16 Tissue DNA Purification Kit (Promega). RNA quality was assessed with the RNA Qubit™ RNA HS Assay Kit (ThermoFisher) ...
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bioRxiv - Neuroscience 2022Quote: ... wherein the 3’ UTR of Prr7 (NM_0010302964) was amplified from mouse DNA and cloned into the pmiRGLO dual-luciferase expression vector (Promega). Mutations in miRNA binding sites conserved across mammals were introduced by site-directed mutagenesis using Pfu Plus! DNA Polymerase (Roboklon).
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bioRxiv - Genomics 2022Quote: ADGRG6_3 and ADGRG_4 sequences were PCR amplified from human genomic DNA and cloned into the pGL4.23 plasmid (Promega, E8411). Human preadipocytes ...
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bioRxiv - Cell Biology 2021Quote: ... Viral RNA was detected using the CDC 2019-Novel Coronavirus Real-Time RT-PCR Diagnostic Panel as per Centre for Disease and Control’s optimised protocol (Integrated DNA Technologies) for use with Go-Taq 1-step RT-qPCR Master Mix (Promega). Gene expression profile and SARS-CoV-2 N subgenomic RNA expression were determined with a standard qPCR cycle (50°C for 2 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Standard procedures for DNA digestion and ligation were used in conditions recommended by the enzyme manufacturer (Promega or Fermentas). All DNA sequencing was carried out by Beckman Coulter or Eurofins Genomics.
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bioRxiv - Cancer Biology 2021Quote: ... Quantification of the libraries was done using Quantus Fluorometer and QuantiFluor Double Stranded DNA System (Promega, Madison, Wisconsin, USA). Libraries were run in the rapid run flow cell and were paired-end sequenced (2×76bp ...
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bioRxiv - Microbiology 2021Quote: ... Ten nanograms of template DNA was added to 5 μl Green GoTaq Reaction Mix Buffer (Promega, Madison, Wisconsin, USA), 1.25 units of GoTaq DNA Polymerase (Promega ...
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bioRxiv - Genomics 2019Quote: ... 2 μL of extracted genomic DNA was added to 25 μL of GoTaq Green Master Mix (Promega, Madison, WI) and 1 μL of 10 μM universal primers 8F (5’- AGAGTTTGATCCTGGCTCAG-3’ ...
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bioRxiv - Microbiology 2019Quote: ... The 1400 bp long DNA fragments were purified (Wizard®SV Gel and PCR Clean-Up System, Promega, Germany) and sequenced using the same 27f and 1492r primer pair ...