Labshake search
Citations for Promega :
1651 - 1700 of 2516 citations for DNA Sequencer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2020Quote: ... and amplified with one of the nine pairs of barcoded FAS_e5_BR_F and FAS_e5_BR_R primers (Baeza-Centurion et al., 2019) and GoTaq flexi DNA polymerase (M7806, Promega). Each pair of primers had a distinct 8-mer barcode sequence to distinguish the nine experimental replicates ...
-
bioRxiv - Genomics 2020Quote: ... 300ng of plasmid DNA for each biological replicate was transfected into HepG2 cells using FuGENE (Promega) in triplicate ...
-
bioRxiv - Biochemistry 2020Quote: ... bacmid DNA containing all four subunits was transfected into Sf9 cells using FuGENE® HD (Promega) per manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... and 4uL 5× GoTaq buffer and 1.25 U GoTaq DNA polymerase (Promega Corporation, Madison, WI, USA) according to the recommended protocol ...
-
bioRxiv - Plant Biology 2019Quote: ... The quality and quantity of extracted DNA was evaluated using a QuantiFluor® dsDNA System (Promega), and by running DNA samples on 1% agarose gel ...
-
bioRxiv - Physiology 2019Quote: ... Eluted DNA as well as pre-IP controls were amplified using GoTaq Green Master Mix (Promega) with primers designed for the promoter regions of Ucp1 and Sarcolipin ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... and the contaminating genomic DNA was removed by two rounds of DNase I treatment (Promega I). Once the absence of genomic DNA was confirmed (by using a 40 cycle long PCR and a primer developed to amplify the H111 genomic DNA ...
-
bioRxiv - Physiology 2019Quote: ... Plasmid DNA (6 µg) was mixed (1 : 3 ratio) with transfection reagent (Fugene 6; Promega, UK) in reduced serum media (OptiMEM ...
-
bioRxiv - Genomics 2019Quote: ... PCR was performed according to the manufacturer’s recommendations (GoTaq DNA polymerase protocol, Promega, Madison, WI, USA). All PCR products were purified using the EPPIC Fast kit (A&A Biotechnology ...
-
bioRxiv - Genomics 2020Quote: ... coli transformants using a Wizard® Plus SV Minipreps DNA Purification System (Promega, Madison, WI, USA). Both RNAi constructs containing Dg vATPase A R1 and R2 were verified by Sanger sequencing ...
-
bioRxiv - Genetics 2021Quote: ... The purified DNA was PCR amplified using 2× GoTaq Colorless Master Mix (Promega, Madison, WI, USA), and PCR product was extracted as above to eliminate primer-dimers ...
-
bioRxiv - Genomics 2021Quote: ... from freshly frozen brain tissues or the Wizard genomic DNA purification kit (Promega, Madison, WI, USA) from blood according to manufacturer instructions ...
-
bioRxiv - Microbiology 2021Quote: ... in the digestion solution and lysis buffer of a Wizard SV 96 Genomic DNA kit (Promega). The samples were centrifuged for 10 min at 16,000 g and the supernatant was transferred to the binding plate ...
-
bioRxiv - Microbiology 2020Quote: ... DNA was extracted with Maxwell® RSC cultured cells kit (Promega Pte. Ltd., Cat. No. AS1620). DNA was cleaned up and concentrated with 0.4× Agencourt AMPure XP beads (Beckman Coulter ...
-
bioRxiv - Biochemistry 2020Quote: ... subjected for mini-prep plasmid purification by Wizard® Plus Minipreps DNA Purification System (Promega, USA), and used as the template in subsequent PCR amplification of the given library ...
-
bioRxiv - Cancer Biology 2020Quote: ... DNA from FFPE material was extracted Maxwell® 16 LEV RNA FFPE Purification Kit (Promega, AS1260).
-
bioRxiv - Biochemistry 2020Quote: ... Point mutations were introduced by PCR mutagenesis using the Pfu DNA polymerase (Promega, Alexandria, VIC, Australia). Cells were transfected using Lipofectamine® 3000 (Thermofisher Scientific ...
-
bioRxiv - Systems Biology 2021Quote: Recovered DNA was quantified via fluorescent quantitation (using either the Invitrogen PicoGreen or Promega QuantIT system), and samples of sufficiently high concentration were also run on a 2% agarose gel for fragment size assessment ...
-
bioRxiv - Molecular Biology 2020Quote: ... Obtained DNA was purified using the Wizard SV Gel and PCR Clean up system (Promega, US) in a final volume of 50 μL ...
-
bioRxiv - Microbiology 2020Quote: ... and the pellets were used for gDNA isolation with the Wizard Genomic DNA Purification Kit (Promega) as described previously (Liu et al. ...
-
bioRxiv - Cancer Biology 2020Quote: ... Standards for quantification were created by serial dilution of pre-quantified BSC human male DNA (Promega). Amplification reactions were performed in duplicate using 96 well-plates using a CFX96 Touch Real-time PCR Detection System (Bio-Rad).
-
bioRxiv - Cancer Biology 2020Quote: The region of interest (750-850 bp) was PCR amplified from pooled male human DNA (Promega) and cloned into a STARRseq luciferase validation vector_ORI_empty plasmid (Addgene plasmid #99298 ...
-
bioRxiv - Cell Biology 2021Quote: ... Complementary DNA (cDNA) was synthesized following manufacturer’s instructions using the ImProm II Reverse Transcriptase system (Promega) from 500 ng of total RNAs for mycelial samples and 650 ng of total RNAs for sheath samples ...
-
HNRNPA2B1 controls an unfolded protein response-related prognostic gene signature in prostate cancerbioRxiv - Cancer Biology 2022Quote: ... DNA and siRNA transfections were performed as detailed in the figure legends using ViaFect (E4981, Promega) and RNAiMax (13778-075 ...
-
bioRxiv - Bioengineering 2022Quote: Genomic DNA was harvested from cells and tissues using the ReliaPrep gDNA Tissue Miniprep System (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... and pCMV-hyPBase was performed using 500 ng each DNA and 6 μl of FugeneHD (Promega) as per manufacturer’s instructions20,49,50 ...
-
bioRxiv - Biochemistry 2022Quote: ... Quality was assessed by the Agilent FEMTOpulse and DNA quantified by the Quantus dsDNA kit (Promega). DNA was processed to obtain a PacBio Sequencing-compatible library following the recommendations outlined in “Procedure & Checklist – Preparing HiFi Libraries from Low DNA Input Using SMRTbell Express Template Prep Kit 2.0” ...
-
bioRxiv - Cancer Biology 2022Quote: ... and transcribed into complementary DNA (cDNA) with the GoScript Reverse Transcription System (Promega, Madison, WI, USA). cDNA was then amplified with the SYBR Green Master kit (Roche ...
-
bioRxiv - Microbiology 2021Quote: ... DNA was extracted from fresh cultures of these 18 strains on an automated platform (Promega Maxwell) as previously described [23] ...
-
bioRxiv - Microbiology 2020Quote: ... 1 μg of genomic DNA from the corresponding isolates was digested by EcoRI (Promega, Madison, WI), the amplified DNA fragments were subjected to ethanol precipitation (65) ...
-
bioRxiv - Microbiology 2020Quote: Plasmid DNA was combined at a 3:1 ratio of FuGENE® 6 Transfection Reagent (Promega) to DNA and incubated 20 min at RT ...
-
bioRxiv - Cell Biology 2020Quote: Genomic DNA was extracted from primary fibroblasts using a commercial purification kit (Promega, Madison, WI, USA). DNA was store in TE (10 mM Tris ...
-
bioRxiv - Molecular Biology 2022Quote: ... The genomic DNA concentration was quantified using the Quantifluor® ONE dsDNA Dye System (E4971, Promega). Genomic DNA (5–20 ng ...
-
bioRxiv - Genomics 2019Quote: ... PCR was performed with gene-specific primer pairs (Table S15) using a DNA polymerase kit (Promega). The PCR conditions were 95 °C for 3 min ...
-
bioRxiv - Plant Biology 2020Quote: ... The final DNA concentration of each pool was measured using the Quantus™ Fluorometer (Promega, Germany) and an equal quantity of DNA (i.e. ...
-
bioRxiv - Pathology 2021Quote: ... with DNA isolated using an EPMotion and the ReliaPrepTM 96 gDNA Miniprep HT System (A2671, Promega). This DNA was used to confirm a sample’s chain of custody throughout the reprogramming process ...
-
bioRxiv - Microbiology 2021Quote: ... The supernatant was discarded and gDNA was extracted using the Wizard Genomic DNA purification kit (Promega) according to vendor’s protocol ...
-
bioRxiv - Immunology 2020Quote: ... Complementary DNA (cDNA) was synthesized from total RNA (500 ng) using M-MLV Reverse Transcriptase (Promega) as per the supplier’s protocol (1 hr ...
-
bioRxiv - Plant Biology 2019Quote: ... Enriched DNA fragments were amplified and cloned using the pGEM-T easy vector (Promega, Madison, USA) and transformed into XL1-BLUE Escherichia coli competent cells (Stratagene ...
-
bioRxiv - Microbiology 2019Quote: ... using the digestion solution and lysis buffer of a Wizard SV 96 Genome DNA kit (Promega). The samples were then centrifuged for 10 min at 16,000g and the supernatant was transferred to the binding plate ...
-
bioRxiv - Genetics 2019Quote: Nucleic acids were extracted from blood samples using the Wizard genomic DNA Promega Kit (Promega Corporation). Multiplex ...
-
bioRxiv - Genomics 2021Quote: ... was extracted from tissue samples using a commercial kit (Wizard R Genomic DNA Purification Kit, Promega) following manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2021Quote: ... Cells were transfected with 2 μg DNA using FuGENE® HD Transfection Reagent (REF E2311, Promega). After 24 hours ...
-
bioRxiv - Genetics 2021Quote: ... RT-PCR was performed by using GoTaq® DNA polymerase (Promega, 2800 Woods Hollow Road·Madison, USA) in an Applied Biosystems™ Veriti™ Thermal Cycler ...
-
bioRxiv - Microbiology 2019Quote: ... or mCherry were amplified by PCR (5’-CCGGGTACCATGGGCAGCAGCCATCATC and 5’-CGGGAATTCTTACTTGTACAGCTCGTCCAT primers; Pfu DNA polymerase, Promega) from the pRGrectac-NHis constructions (Fernández-Tresguerres et al. ...
-
bioRxiv - Microbiology 2020Quote: ... Fugene HD (Fugene HD [μL]:DNA [μg]:DMEM [μL] = 5:2:200;Promega, Madison, WI, USA) was used for transient transfection ...
-
bioRxiv - Molecular Biology 2021Quote: Plasmids for preparing DNA templates for in vitro transcription were assembled using the pGEM-11Zf(+) (Promega) backbone ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... and isolation of gDNA was carried out with a Wizard Genomic DNA Purification Kit (Promega, USA) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... any amount of DNA was removed using DNase (RQ1 RNase-Free DNase, Promega, Madison, Wisconsin, USA) and measured using Qubit (Invitrogen ...
-
bioRxiv - Microbiology 2022Quote: ... Bacteria were grown overnight in 5mL MB and DNA extracted using an extraction kit (Wizard, Promega) according to the manufacturer’s instructions ...