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Citations for Promega :
1551 - 1564 of 1564 citations for 2x SYBR Green qPCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Photoreceptor disc incisures form as an adaptive mechanism ensuring the completion of disc enclosurebioRxiv - Cell Biology 2023Quote: ... Samples containing 20 µg of protein were mixed with 0.5 µg BSA (used as an internal standard in this analysis) and cleaved with 1 µg trypsin/LysC mix (Promega, Madison, WI) using the SP3 beads protocol described in (Hughes et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... Digestion was performed in the S-Trap overnight at 37°C using Trypsin/Lys-C Mix Mass Spec Grade (Promega, Walldorf, Germany), followed by elution in 50 mM TEAB ...
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bioRxiv - Genomics 2023Quote: ... 6.10^5 S2 cells were transfected with a mix consisting of 100ng of luciferase plasmid and 5ng of Renilla plasmid per well using FuGENE® HD (Promega, #E2311). At least tree biological l replicates were measured per construct ...
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bioRxiv - Developmental Biology 2024Quote: ... The transfection mix included 0.5 μg of pGL3-enhancer plasmids with firefly luciferase gene sequences driven by an SV40 promoter (Promega, catalog no. E1761), 0.02 μg of pRL-TK Renilla luciferase (Promega ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated with compound at 37°C for 72h before multiplexed quantitation of toxicity using CellTox green™ and cell viability using Cell-TiterGlo® (both assay kits from Promega Corp, Madison WI). As a positive control for toxicity 24h prior to cessation of the assay 0.5 mM NaN3 was added to half of the vehicle wells to induce cell death ...
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bioRxiv - Physiology 2020Quote: ... The beads were resuspended in 10 μl of reverse transcriptase mix (100□U, SuperScript II, Invitrogen; 10 U, RNAsin, Promega, Madison, Wisconsin, USA), 1□×□Superscript II First-Strand Buffer ...
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bioRxiv - Plant Biology 2020Quote: ... approximately 0.6 mg IgG-enriched proteins were first digested on the beads with mass spectrometry-grade Trypsin/Lys- C Mix (Promega, Madison, WI, USA) at a 1:50 (enzyme/substrate ...
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bioRxiv - Genomics 2021Quote: ... We added tagmentation buffer (1x TD buffer, 0.015 µL TTE Mix V50 [Vazyme, #TD501], 0.625x protease inhibitor cocktail [Promega, #G6521], and 1 mM MgCl2) to reach a volume of 10 µL per well and then incubated the plates at 55 °C for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... then dipped in a well of a 96-well PCR plate filled with 20 µl of a gyrB PCR mix (4 µl of 5x GoTaq buffer (Promega, Madison, WI, USA), 0.4 µl of 10mM dNTP ...
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bioRxiv - Microbiology 2022Quote: ... then 1 µL was transferred to a PCR tube containing 500 nM of each forward and reverse primer and 1X GoTaq mix (M7123, Promega, Madison, WI, USA) in 20 µL total volume ...
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bioRxiv - Cell Biology 2022Quote: Digoxin-labeled RNA probes were in vitro transcribed as per manufacturers’ instructions (DIG RNA labeling Mix, Roche; T7 polymerase, Promega; SP6 Polymerase, Promega) and purified by G-50 column (GE Healthcare) ...
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bioRxiv - Cell Biology 2023Quote: Affinity purified proteins retained on beads were resuspended in 100 µL of NH4HCO3 at 25 mM containing 2 µg of Trypsin/Lys-C mix (Mass Spec Grade, Promega, Madison, WI, USA). Digestion was performed under agitation at 37°C during 4h ...
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bioRxiv - Molecular Biology 2024Quote: ... Seventy micrograms of protein were digested overnight using the filter-aided sample preparation (FASP) method 23 with Trypsin/Lys-C mix (Promega, Madison, WI, USA) (enzyme-to-protein ratio 1:35 ...
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bioRxiv - Immunology 2022Quote: ... and digested over-night with Lys-C-Trypsin mix (1:100 enzyme to protein ratio) and trypsin (Promega; 1:50 enzyme to protein ratio). Following the digestion step ...