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Citations for Promega :
1301 - 1350 of 1464 citations for 2x SYBR Green qPCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The isolated RNAs (20 ng) were reverse-transcribed and quantitative PCR (qPCR) were perform using GoTaq Probe 1-Step RT-PCR system (Promega) in a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: ... Gene expression was assessed by qPCR using primers listed in the supplemental material Table S6 and the GO Taq PCR kit (Promega). Gene expression was quantified using SYBR Green (Roche ...
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bioRxiv - Immunology 2023Quote: ... cDNA was generated from 20-100 ng of RNA using the GoTaq 2-step RT-qPCR System (Promega, cat# A6110). qPCR was performed with SYBR Green on a StepOnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2024Quote: The copy number of re-integrant strains was determined by extracting the genomic DNA and performing quantitative PCR (qPCR) using GoTaq polymerase (Promega) and a StepOnePlus real-time PCR machine (ThermoFisher) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... QRT-PCR reactions were performed by using the GoTaq®1-Step RT-qPCR System (Promega Scientific, Madison, WI, USA) with Bio-Rad CFX Connect (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2022Quote: ... and proteins were subjected to overnight digestion with mass spec grade Trypsin/Lys-C mix (Promega). Following digestion ...
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bioRxiv - Plant Biology 2021Quote: ... 8 µL of a mix consisting of 2 µL deoxyribonucleotide triphosphate (dNTPs, Promega, Madison, Wisconsin, US), 4 µL 5x RT-buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... packaging mix was co-transfected with lentiviral vector in HEK293FT using FuGENE HD Transfection Reagent (Promega). Media containing transfection reagent was replaced with complete growth media after 24 hours ...
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bioRxiv - Biochemistry 2021Quote: ... and proteins were subjected to overnight digestion with Mass Spec Grade Trypsin/Lys-C mix (Promega). The beads were then pulled down and the solutions containing the digested peptides were desalted using AssayMap C18 cartridges mounted on an AssayMap Bravo liquid handling system (Agilent Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription was conducted using GoScriptTM Reverse Transcription Mix and Random Primers (A2800, Promega, WI, USA) according to the manual ...
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bioRxiv - Plant Biology 2023Quote: ... proteins were alkylated with iodoacetamide and digested with Trypsin/Lys-C Mix (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... and digested overnight at 37°C with a 2 μg Trypsin/Lys-C enzyme mix (Promega). The next day ...
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bioRxiv - Microbiology 2023Quote: ... then digested with 4 μL of a trypsin/Lys-C Mix (0.5 μg/uL) (Promega # V5071) using a two-step in-solution digestion overnight at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and subjected to in-gel digestion with trypsin/Lys-C Mix (V5071, Promega, Madison, WI, USA) at 37°C for 12 h ...
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bioRxiv - Biochemistry 2023Quote: ... A mixture of trypsin and Endo-Lys-C (Trypsin/Lys-C Mix, Mass Spec Grade, Promega) was used for digestion of the sample ...
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bioRxiv - Biochemistry 2023Quote: ... A mixture of trypsin and Endo-Lys-C (Trypsin/Lys-C Mix, Mass Spec Grade, Promega) was used for digestion of the sample ...
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bioRxiv - Molecular Biology 2023Quote: ... 1/20 of the cDNA was amplified by PCR using the GoTaq DNA polymerase mix (Promega) with specific antigenome primers (Appendix Table S1) ...
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bioRxiv - Microbiology 2021Quote: ... Positive constructs were identified by colony PCR using the segment-specific PCR primers and Taq green mastermix (Promega) with the following cycle profile ...
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bioRxiv - Genomics 2019Quote: ... PCR was prepared using 5X GoTaq Green Reaction Buffer and GoTaq DNA Polymerase (Promega, Madison, WI, cat# M3005), 10 mM dNTPs ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Real-Time PCR Diagnostic Panel17 and a protocol for quantifying the SARS-CoV-2 subgenomic E gene RNA (E SgRNA)18 using the GoTaq® Probe 1-Step RT-qPCR System (Promega). For quantification of SARS-CoV-2 using the nCoV assay ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Real-Time PCR Diagnostic Panel17 and a protocol for quantifying the SARS-CoV-2 subgenomic E gene RNA (E SgRNA)18 using the GoTaq® Probe 1-Step RT-qPCR System (Promega). For quantification of SARS-CoV-2 using the nCoV assay ...
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bioRxiv - Microbiology 2022Quote: ... then diluted 1:5 with nuclease-free water before 2 µL was used as template in a 10 µL GoTaq 1-Step RT-qPCR (Promega, A6020) reaction (69) ...
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bioRxiv - Neuroscience 2021Quote: ... reverse transcription of viral RNA followed by quantitative PCR was performed with GoTaq® Probe 1-Step RT-qPCR System (Promega) on a 7500 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Amplification reactions were run in a 20 μL volume using the GoTaq 1-Step RT-qPCR System (Promega, Madison WI, USA) and the recommended manufacturer’s instructions as described in Cervera et al ...
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bioRxiv - Plant Biology 2022Quote: ... 6.5 μl of 1:100 diluted cDNA per reaction were used with the GoTaq® qPCR Mastermix (Promega GmbH, Madison, USA) in a total volume of 15 μl ...
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bioRxiv - Cancer Biology 2023Quote: ... washed with PBS and inflammasome activation was assessed using qPCR or inflammasome caspase-1 activity was measured using Caspase-Glo® 1 inflammasome assay kit (G9951; Promega) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: The viral RNA derived from the lung and nasal turbinate samples was quantified using a protocol for quantifying the SARS-CoV-2 sub-genomic E gene RNA (sgE)29 using the GoTaq® Probe 1-Step RT-qPCR System (Promega).
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bioRxiv - Molecular Biology 2024Quote: ... and gene expression was determined by quantitative PCR (QuantStudio 6 Pro Real-Time PCR System) with GoTag qPCR System (Promega, A6002). Actb and Rplp0 were used as housekeeping genes ...
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bioRxiv - Plant Biology 2022Quote: ... 100 ng total DNA was added to a 20 μl PCR containing 10 μl GoTaq® Master Mixes (Promega, USA) and 0.1 μM of each primer pair ...
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bioRxiv - Microbiology 2021Quote: Peptides from cell-surface/biofilm-ECM-associated proteins were released via a Trypsin/Lys-C mix (Promega) at a final concentration of 50 ng µL-1 ...
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bioRxiv - Microbiology 2020Quote: ... polymerases and Gibson mix reagents were used according to the manufacturer’s instructions (Promega and New England Biolabs). Plasmids were verified by PCR and Sanger sequencing (Eurofins).
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized from 800 ng total RNA using GoScriptTM Reverse Transcription Mix using oligo(dT) (Promega). Real-time quantitative PCR was performed by the Taq-Man (rice ...
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bioRxiv - Microbiology 2020Quote: ... The reaction was stopped by the addition of 5.5μL of a mix containing 5% SDS and 0.25M EDTA and deproteinized with proteinase K (Promega) for 1hour at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... samples were adjusted to 3 mM EDTA and digested with 0.5 μg Trypsin/LysC mix (Promega #V5073) for 1h at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Instrument quality control was monitored using the Promega 6×5 LC-MS/MS Peptide Reference Mix (Promega) before and after each MS experiment run ...
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bioRxiv - Synthetic Biology 2021Quote: 1μl of 50μM oligo d(T)20 (IDT) and 1μl of 10mM dNTP mix (Promega cat #U1240) were combined with 1 μg of RNA before adding dd H20 up to a final volume of 13μl ...
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bioRxiv - Immunology 2021Quote: ... 50 mM Tris-Cl (pH 8) prior to digestion with Trypsin/Lys-C Mix (Promega, Madison, WI) at a ratio of 1:25 (enzyme ...
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bioRxiv - Systems Biology 2023Quote: ... then proteins were digested with 1 μg of mass spectrometry grade Trypsin/Lys-C enzyme mix (Promega) in 50 μL of digestion buffer (100 mM ABC ...
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bioRxiv - Plant Biology 2023Quote: ... and dNTP mix at a final concentration of 0.5 mM of each dNTP (Cat. No. U1511; Promega). Semi-quantitative RT–PCR amplifications were performed on 1 μl of cDNA in a 12.5-μl reaction volume to start with ...
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bioRxiv - Molecular Biology 2023Quote: ... on-column trypsin digestion was performed by adding by mass spectrometry grade trypsin/Lys-C mix (Promega) in a 50 mM NH4HCO3 buffer overnight at 37 °C.
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bioRxiv - Cell Biology 2023Quote: ... Samples were adjusted to 3 mM EDTA and digested with 1.0 μg Trypsin/LysC mix (Promega #V5073) for 1 h at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids containing rsbU or rsbT/rsbUY28I were subjected to PCR mutagenesis using GoTaq DNA polymerase mix (Promega) supplemented with 2mM additional MgCl2 ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were adjusted to 3 mM EDTA and digested with 0.5 μg Trypsin/LysC mix (Promega #V5073) for 1h at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR mixture was at a volume of 10 μL containing 5 μL SYBR Premix Ex Taq (Promega, Madison, USA), 0.5 μM each of the primers ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1 µl of this crude lysate was used for PCR in a 10 µl GoTaq Green reaction (Promega M7123) with 0.25 µM of primers ...
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bioRxiv - Genomics 2022Quote: ... 2 µl of yeast lysate was used as a template in a 10 µl GoTaq Green reaction (Promega M7123) with 0.25 µM of primers ...
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bioRxiv - Epidemiology 2019Quote: ... The 16S rDNA touchdown PCR was performed in 20 µL reactions containing 1× GoTaq® Green Mastermix (Promega, USA), 0.4 µM of each primer SpirRNAF and SpirRNAR (S2 Table ...
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bioRxiv - Cancer Biology 2022Quote: PCR to amplify the region flanking the editing site of COPA (Ile164Val) was performed in a total volume of 50μl containing 1x Green Go TaqR Flexi Buffer (Promega), 2 mM MgCl2 solution ...
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bioRxiv - Microbiology 2023Quote: ... USA) was performed using primer 27F on a V1-V3 16S rRNA gene colony-PCR amplicon (GoTaq Green, Promega) of primers 27F and 519R ...
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bioRxiv - Genomics 2023Quote: ... 2 µL of yeast lysate was used as a template in a 10 µL GoTaq Green reaction (Promega M7123) with 0.25 µM of primers ...