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Citations for Promega :
1251 - 1300 of 1464 citations for 2x SYBR Green qPCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Proteins were digested for 18 h with trypsin/Lys-C Mix (Promega Corporation, USA) and 10 μg of each sample was used for proteome analysis ...
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bioRxiv - Cell Biology 2023Quote: ... and cut finely prior to in-gel digestion with Trypsin/Lys-C Mix (Promega) at 37 °C for 12 h ...
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bioRxiv - Cell Biology 2023Quote: ... 25U of RNAsin ribonuclease inhibitor and 500µM dNTP deoxynucleotide mix (all reagents from Promega). Gene expression of CD90 ...
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bioRxiv - Plant Biology 2023Quote: ... Samples were digested in fresh digestion buffer containing 1 μg Typsin/LysC mix (Promega) and 0.01% ProteaseMAX (Promega ...
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bioRxiv - Molecular Biology 2024Quote: ... An equal amount of extension mix consisting of avian myeloblastosis virus reverse transcriptase (Promega), 5x AMV buffer and 40 μM dNTPs was added ...
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bioRxiv - Cancer Biology 2022Quote: Efficacy of compounds was validated by applying the real-time CellTox™ Green Cytotoxicity assay (Promega). Assays were performed according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 µL of PBS containing 4 μg/mL Hoechst and 1/10000 CellTox Green Dye (Promega) were were dispensed per well 1 h prior to imaging at Cytation5 image cytometer or Opera Phenix (Perkin Elmer ...
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bioRxiv - Microbiology 2020Quote: ... or as changes in cell membrane integrity using CellTox Green Cytotoxicity Assay (cat.no. G8743, Promega, USA). Experiments were performed according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... 20 µL of PBS containing 10 μg/mL Hoechst and 1/4000 CellTox Green Dye (Promega) were dispensed per well 8 h prior to imaging at Opera Phenix (Perkin Elmer ...
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bioRxiv - Immunology 2023Quote: ... The assay was performed at 16hr post infection using the CellTox Green Cytotoxicity Assay Kit (Promega). Fluorescence readings were recorded as triplicates using a Tecan Infinite M1000 Quadruple Monochromator Microplate Reader ...
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bioRxiv - Cancer Biology 2023Quote: ... organoids were stained using Hoechst 33342 (1 μg/mL) and CellTox Green (Promega, 1/20 000) dyes for 8 h prior to imaging at Opera Phenix (Perkin Elmer ...
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bioRxiv - Genomics 2024Quote: ... (i) quantification of DNA samples of multiple sexed green toads (QuantiFluor® dsDNA System kit, Promega), (ii ...
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bioRxiv - Microbiology 2020Quote: ... qPCR was then performed to detect viral gene ORF26 and luciferase from the carrier plasmid using GoTaq polymerase (Promega) and the ΔΔCT method ...
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bioRxiv - Microbiology 2023Quote: ... RNA reverse transcription and cDNA amplification was then carried out by the GoTaq 1-Step RT-qPCR System (Promega), using established primers (31) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µL per input or ChIP sample was used in a qPCR using the GoTaq® Mastermix (#M7133, Promega) and primer pairs for the amplification of the Ifnb1 promoter region (ChIP_IFNb1_fwd 5’-GCCAGGAGCTTGAATAAAATG ...
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bioRxiv - Physiology 2023Quote: ... The obtained cDNA was used as the template for quantitative real-time PCR (qRT-PCR) using qPCR Mastermix (Promega). mRNAs expression levels were normalized to actin-5C mRNA used as the internal control The relative level for each gene was calculated using the 2-DDCt method (Hulf et al. ...
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bioRxiv - Cell Biology 2023Quote: ... The obtained cDNA was used as the template for quantitative real-time PCR (qRT-PCR) using qPCR Mastermix (Promega). mRNAs expression levels were normalized to actin-5C mRNA used as the internal control ...
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... Orsay Virus RNA1 level was quantified by qPCR using 1 μl of 1/5 diluted cDNA (GoTaq Promega A6001) and run on QuantStudio 3 Real Time PCR system ...
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... Orsay virus RNA1 level was quantified by qPCR using 1 μl of 1/5 diluted cDNA (GoTaq Promega A6001) and run on QuantStudio 3 Real Time PCR system (RNA1 qPCR primers GW194 and GW195)23.
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested overnight at 37 °C with Trypsin/Lys-C Mix (Promega, Charbonnières, France) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... proteins on the beads were digested by adding 400 ng trypsin/Lys-C mix (Promega) for 16 h at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Reaction mix was diluted twice with nuclease-free water (Nuclease-Free water, Cat# P119C, Promega) and 1 ul of amplified dsDNA was used for gene specific TaqMan Assay using the manufacture protocol for TaqMan™ Fast Advanced Master Mix (Cat# 4444964 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins on the beads were digested with 200 ng trypsin/Lys-C mix (Promega, V5072) for 16 h at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... 1x protease inhibitor mix (Benzamidine & PMSF)) supplemented with 7 U/ml DNase I (M610A, Promega) and lysed by Dounce homogenization and incubation on ice for 30 min ...
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bioRxiv - Microbiology 2022Quote: ... Neutravidin-bound proteins were digested on bead with 400 ng Trypsin/Lys-C mix (Promega) at 37 °C overnight with shaking ...
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bioRxiv - Systems Biology 2022Quote: ... 500 µg of proteins were digested with trypsin/Lys-C mix (V5071 or V5072, Promega) overnight at 37 °C with a 1:50 enzyme to protein ratio ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins bound to beads were digested with 1 μg of Trypsin/Lys-C mix (Promega) for 3 h at 37 °C and then supplemented with and additional amount of 150 ng Trypsin/Lys-C for overnight digestion on an end-over-end rotation apparatus at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative PCR was performed in a BioRad C1000 Touch thermocycler using GoTaq mix (Promega #A6001) [32] ...
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bioRxiv - Biochemistry 2022Quote: ... smegmatis Complex I (8 μg) was digested with MS-grade trypsin/Lys-C mix (Promega) at a 25:1 (w/w Complex I:protease ratio in 25 mM Tris-HCl ...
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bioRxiv - Plant Biology 2022Quote: ... the 40 µl in vitro translation reactions contained 20 µM 19 amino acid mix (Promega) and 0.45 µCi/µl of [35S]-methionine (Hartmann Analytics) ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were adjusted to 3mM EDTA and digested with 0.2μg Trypsin/LysC mix (Promega #V5073) for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... and then digested overnight with 300 ng of Trypsin/Lys-C Mix (Promega, Madison, WI). For on-bead digestion following SA pull-down ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and digestion with Trypsin and Lys-C (Trypsin/Lys-C Mix, Mass Spec Grade Promega) for 14 hours at 37° C.
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bioRxiv - Cancer Biology 2023Quote: ... we added 500 ng of trypsin/Lys-C Mix (CAT# V5072, Promega, Madison, WI, USA) to the beads soaked in 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cancer Biology 2023Quote: ... In this time the Fugene HD mix (8.6 μl Fugene HD (Promega, Madison, WI, USA) in 110 μl Opti-MEM was prepared and mixed thoroughly ...
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bioRxiv - Biochemistry 2023Quote: ... followed by 500 ng of trypsin/Lys-C Mix (CAT# V5072, Promega, Madison, WI, USA) and mixed gently at 37°C overnight to digest proteins ...
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bioRxiv - Systems Biology 2023Quote: ... 1 µL of enzyme mix (lysC & trypsin, 10 ng/µL in water, Promega, Cat. V5072) was dispensed into each well by using the pL-volume dispensing function of the cellenONE at 20°C and 85% humidity ...
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bioRxiv - Zoology 2023Quote: ... The mix contained 12.5 µL of GoTaq® G2 Hot Start Polymerase (Promega,, Wisconsin, USA), 1 µL of degenerated primers at 10 µM (HAN-L-F1 and HAN-L-R1 for primary PCR ...
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bioRxiv - Plant Biology 2021Quote: ... We added 300μl of Proteinase K Buffer 2X (200 mM Tris-HCl pH7.5; 100 mM NaCl; 20 mM EDTA; RNasin PROMEGA 20 U/μl; 0.04 mg/ml Proteinase K) and incubated for 1 h at 55 °C ...
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bioRxiv - Biochemistry 2021Quote: ... The dried resin was resuspended with 50 mM Tris pH 7.5 (2x resin volume) and 200 ng of trypsin was added (Promega; made up to 100 ng·μL-1 in H2O), the samples were digested overnight shaking at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... Samples were ground to fine powder using pestles on dry ice and then 100 μl 2x cell culture lysis buffer was added (Promega, E1531; with 150 mM Tris-Hcl pH7.5). 50 μl of leaf extract was mixed with 50 μl luciferase substrate (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR amplification was performed in 25 μl reactions using 5 μl X Green GoTaq Flexi Buffer (Promega), 1.5 μl MgCl2 (25mM ...
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bioRxiv - Biophysics 2020Quote: ... samples from homozygous HaloTag-TEV mice were incubated overnight with 5 μM HaloTag Oregon Green ligand (Promega) in Phosphate-buffered saline (PBS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Compound cytotoxicity in feline cells was assessed using the commercially available kit (CellTox Green Cytotoxicity Assay, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1ul of yeast lysate was used as template in a 10 µl GoTaq Green reaction (Promega M7123) with 0.25 µM of primers ...
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bioRxiv - Neuroscience 2021Quote: ... Grapefruit Integropectin cytotoxicity was assessed by CellTox™ Green Cytotoxicity Assay (Promega Corporation, Madison, WI 53711 USA). At the end of treatment ...
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bioRxiv - Cancer Biology 2020Quote: ... cell viability and cytotoxicity were measured with CellTiter-Glo®2.0 and CellTox™-Green assays (Promega) respectively according to the manufacturer’s instructions with a ClarioStar plate reader (BMG Labtech) ...
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bioRxiv - Microbiology 2021Quote: High molecular weight DNA was extracted using the Maxwell® RSC PureFood GMO and Authentication Kit as for qPCR (Promega). The high molecular weight DNA was sent to the Functional Genomic Center Zurich (FGCZ ...
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bioRxiv - Pathology 2021Quote: RT-qPCR (SARS-CoV-2 and MS2 viral genome detection) were performed with the GoTaq 1-step qRt-PCR kit (Promega) using 3.8µL of extracted RNA and 6.2µL of RT-qPCR mix that contains 250nM of each primer and 75nM of probe ...
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bioRxiv - Microbiology 2022Quote: ... standard curves were generated for each qPCR run from known copy numbers of a linearized pGEM-T Easy plasmid (Promega) containing the amplified PCR product to calculate the number of absolute copies in the samples.