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Citations for Promega :
1401 - 1450 of 1464 citations for 2x SYBR Green qPCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: ... The 25 µL polymerase chain reaction (PCR) mixture was the same for both mtDNA genes using 5x Green GoTaq® Flexi buffer (Promega), 0.2 mM dNTPs ...
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bioRxiv - Plant Biology 2020Quote: ... All PCR amplifications were assembled on ice in 25 μl reactions using 5 μl of 5X Green GoTaq buffer (Promega M3001), 0.5-1 μl of each primer (10 μM) ...
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bioRxiv - Zoology 2022Quote: ... and the other for assessing DNA content (to calibrate the ATP content by the number of sperm cells) using CellTox™ Green Cytotoxicity Assay kit (Promega). After the samples were prepared according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCRs were performed in a final volume of 25 µL with 12.5 µL of GoTaq Green Mastermix (Promega, Madison WI, USA), 9.5 µL of water ...
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bioRxiv - Cancer Biology 2024Quote: The target sites for each crRNA were PCR amplified from genomic DNA extracted from tumor-bearing lungs using GoTaq Green® Mastermix (Promega) and the following primer pairs:
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bioRxiv - Neuroscience 2023Quote: ... These reactions were assembled to a final volume of 20 μL using 5X Green GoTaq® Reaction Buffer and dNTPs (Promega) with laboratory-purified DNA Taq Polymerase ...
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bioRxiv - Molecular Biology 2020Quote: ... purified amplicons from each single-plex TaqMan® qPCR were cloned into a plasmid vector using the pGEM®-T Easy Vector System I kit (Cat.# A1360, Promega, Walldorf, Germany) and One Shot® TOP10 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... The fragment sizes provided by the Fragment Analyzer were compared to the degradation indices obtained from real-time quantitative polymerase chain reaction (qPCR) with PowerQuant System (Promega Corporation, Madison, WI, USA). After one year of storage in − 20 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 μl of Reverse Transcription Mix (10 μl of 10 mM dNTPs and 40 μl of 5X MMLV RT Buffer Promega), 1 μl of 10 mM ddTTP for the sequencing reaction and 1 μl of MMLV Reverse Transcriptase RNAse H minus (Promega ...
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bioRxiv - Systems Biology 2020Quote: ... pH 8.0 and digested for 16 hours on a shaker at 37 °C with a 1:40 ratio of Trypsin/Lys-C mix (Promega). Each sample was de-salted using HLB plates (Oasis HLB 30μm ...
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bioRxiv - Biophysics 2020Quote: ... 1 mM EGTA, 5% glycerol) and freshly supplemented reagents (1 mM DTT, 1 mM ATP, 1 mM PMSF, protease inhibitor mix (Promega) and 1% Tween 20) ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were then diluted to 6 M urea with 50 mM Tris-HCl pH 8 and Trypsin/Lys-C mix (Promega) was added to an enzyme:substrate ratio of 1:25 (w/w ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were then diluted to 4 M urea with 50 mM Tris-HCl pH 8 and Trypsin/Lys-C mix (Promega) was added to an enzyme:substrate ratio of 1:250 (w/w ...
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bioRxiv - Microbiology 2022Quote: ... was added to a final concentration of 3 mM followed by the addition of 5 µl of T7 Enzyme Mix and 50 U RNasin RNase Inhibitor (Promega). To produce transcripts for mock transfections the GTP concentration was increased to 7.5 mM and cap analogues were not added ...
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bioRxiv - Biochemistry 2019Quote: A commercial K562 cell digest supplemented with the 6 x 5 LC-MS/MS Peptide Reference Mix (QC4Life Reference Standard, Promega) was used as quality control (QC ...
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bioRxiv - Microbiology 2020Quote: ... polymerases and Gibson assembly mix reagents were used to manipulate DNA fragments and plasmids according to manufacturer instructions (New England Biolabs and Promega).
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bioRxiv - Microbiology 2020Quote: ... All samples were digested overnight at 37 °C using a Trypsin/Lys-C Mix (mass spec grade, Promega, Madison, WI) (enzyme/substrate of 1:100 w/w ...
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bioRxiv - Cell Biology 2022Quote: Digoxin-labeled RNA probes were in vitro transcribed as per manufacturers’ instructions (DIG RNA labeling Mix, Roche; T7 polymerase, Promega; SP6 Polymerase, Promega) and purified by G-50 column (GE Healthcare) ...
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bioRxiv - Biochemistry 2023Quote: ... Samples containing 20-30 pmol rhodopsin were mixed with 100-250 fmol BSA and cleaved with 1 μg trypsin/LysC mix (Promega) using the SP3 beads protocol described in [29].
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bioRxiv - Molecular Biology 2023Quote: ... Reduction, alkylation and in-gel digestion were performed as described (Shevchenko et al., 2006) except that Trypsin/LysC mix (Promega) was used instead of trypsin ...
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bioRxiv - Cell Biology 2022Quote: ... Tris 50mM pH8.0 and samples were heated 5 min at 90°C before digestion in a mix of 500 ng of LysC (Promega) at 37°C for 2h ...
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bioRxiv - Evolutionary Biology 2023Quote: ... in which proteins were reduced (DTT) and alkylated (IAA) before the digestion with Trypsin and Lys-C (Trypsin/Lys-C Mix, Mass Spec Grade Promega) for 14 hours at 37° C ...
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bioRxiv - Cancer Biology 2023Quote: ... The following day 100 μl of each sample was transferred to a white 96-well plate and 100 μl of the the mix of Bright-GloTM Luciferase Assay (Promega) was added to each well ...
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bioRxiv - Neuroscience 2024Quote: ... the bead and protein mixtures were washed twice with 80% ethyl alcohol and once with 100% ACN before being resuspended in 95 µL NH4HCO3 prior to overnight on-bead digestion (enzyme–protein ratio of 1:20) at 1 000 rpm at 37 °C using modified porcine trypsin/Lys-C Mix (Mass Spectrometry Grade, Promega). The digestion was stopped using trifluoroacetic acid (TFA ...
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bioRxiv - Molecular Biology 2024Quote: ... 40 µL of digestion solution (1.25 mM TCEP, 5 mM chloroacetamide, 0.2 µg trypsin/Lys-C mix (Promega, cat#V5073), in 100 mM HEPES pH 8.5 ...
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Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55BbioRxiv - Neuroscience 2023Quote: ... On-column digestion was performed by incubating 60 µL (1.5 µg) Trypsin/Lys-C Mix (Mass Spec Grade; Promega, UK) in 50 mM TEABC solution at pH 8 on the samples on a thermomixer at 47°C for 1 hour before reducing the incubation temperature to 22°C for overnight digestion ...
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bioRxiv - Developmental Biology 2020Quote: ... primers were used to amplify the PCR product (fwd 5’-GCTGTFATAGGGTGGAGGTG-3’, rev 5’GCTATCAACGCCATTGTGAA-3’) using 1X GoTaq Green (Promega, Madison, WI) with a final primer concentration of 0.2uM ...
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bioRxiv - Molecular Biology 2023Quote: ... HAP1 and HAP1-dEGFPY66S cells were imaged in imaging medium (FluoroBrite™ DMEM, 10% FBS, 2.5 mM CaCl2) supplemented with 2 µL CellTox™ Green (Promega, G8741) and 5 µL Annexin V-AV647 (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... The PCR reaction mixture was prepared for a 10 μL reaction volume using a GoTaq® DNA Polymerase PCR mix (Promega): 2 μL 5X Green GoTaq® Reaction Buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... The PCR reaction mixture was prepared for a 10 μL reaction volume using a GoTaq® DNA Polymerase PCR mix (Promega): 2 μL 5X Green GoTaq® Reaction Buffer ...
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bioRxiv - Genetics 2021Quote: ... and then resuspended in tagmentation reaction mix for 30 min (2.5uL TD1, 1X TD Buffer, Illumina Nextera DNA, FC-121-1030; .25uL 1% digitonin, Promega G9441; 1xPBS;) gently shaking (300rpm on Eppendorf thermomixer ...
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bioRxiv - Neuroscience 2021Quote: The protein lanes were excised from the gels and proteolytic digestion with trypsin/lys-C mix (V5073, Promega, Southampton, United Kingdom) was performed as described (Shevchenko et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... In vitro translation reactions were carried out with 5 ul of either supplemented rabbit reticulocyte lysate or oocyte extract in total volume of 20 µl containing 10 µM amino acid mix (Promega, L4461), 0.5 units SUPERase•In ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 μL of cell lysate was distributed to mix with 25 μL of Nano-Glo Luciferase Assay System (Promega, Cat.# N1120), and another 25 μL of cell lysate was mixed with 25 μL of ONE-Glo Luciferase Assay System (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... 8uL of reaction was mixed with 8uL of Nano-Glo buffer/substrate mix following the Nano-Glo Luciferase Assay System (Promega N1110) instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were digested at 47 °C for two hours with 600 ng of mass spectrometry grade Trypsin/LysC mix (Promega, V5113). The digested peptides were then eluted with two 35 µl increments of 0.2% formic acid in water and two more 40uL increments of 80% acetonitrile with 0.2% formic acid in water ...
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bioRxiv - Bioengineering 2024Quote: ... were taken for overnight digestion with mass spec grade Trypsin/Lys-C Mix (Promega, Madison, WI, cat. No. V5071, 1:20). Samples were reduced with TCEP (final concentration 5 mM ...
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bioRxiv - Cancer Biology 2024Quote: ... cell viability and cytotoxicity were measured with CellTiter-Glo Luminescent Cell Viability Assay with CellTox Green Cytotoxicity Assay (Promega kits G7570 and G8741).
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bioRxiv - Microbiology 2020Quote: ... cells were transfected according to manufacturer instruction with a transfection mix containing a ratio of 1 µg of plasmid for 2 µl FuGene HD transfection reagent (Promega, REF E2311) diluted in DMEM without FCS ...
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bioRxiv - Molecular Biology 2021Quote: ... 5’int-F and 24nt-6stp-R primers and 2) 3’KI: 24nt-6stp-F and 3’int-R using the GoTaq DNA polymerase mix (Promega, Madison, WI) and 200 nM of each primer ...
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bioRxiv - Neuroscience 2021Quote: ... 3 biological replicas were collected to extract the total RNA and the cDNA was synthetized using GoScript™ Reverse Transcription Mix (Promega A2790). Primer pairs used for the qPCR are listed in Table S10.
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bioRxiv - Microbiology 2019Quote: ... Reverse transcription was performed with GoScript™ reverse transcriptase mix random hexamers according to the manufacturer’s protocol (A2800; Promega AG, Dübendorf, Switzerland) using 200 ng of total RNA ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation with 100 μL 50mM ammonium bicarbonate (MilliporeSigma Cat. #A6141) addition of 1 μg trypsin/lysC mix (Promega, Cat. # V5071) at 37 °C for 16 hours ...
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bioRxiv - Microbiology 2022Quote: ... The PCRs were performed in a final volume of 20 μl of amplification mix containing 1 U of GoTaq polymerase (Promega, Madison, WI), 1× GoTaq buffer ...
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bioRxiv - Zoology 2022Quote: ... The mixture was diluted with 150 μL of 50 mM Tris-HCl pH8.0 and digested by adding 500 ng Trypsin/Lys-C mix (Promega, Madison, WI, USA) overnight at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then digested overnight at 35ºC using Trypsin/Lys-C (Trypsin/Lys-C Mix, Mass Spec Grade, Promega, Cat No: V5072); the enzyme-substrate ratio of 1:70) ...
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bioRxiv - Systems Biology 2023Quote: ... proteins were precipitated with isopropanol50 and digested overnight at 37°C with 1:50 enzyme : protein ratio by Trypsin/Lys-C Mix (Promega GmbH, Germany). Resulting peptides were desalted on MicroSpin column (The Nest Group ...
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bioRxiv - Molecular Biology 2023Quote: ... Japan) at 37 C for 2 h before digestion overnight with 1 ug Trypsin/Lys-C mix (V5071, Promega, Madison, WI, USA) at 37 C ...
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bioRxiv - Cell Biology 2023Quote: ... Samples containing 5-10 µg of protein were mixed with 0.25-0.5 µg BSA (used as an internal standard in this analysis) and cleaved with 1 µg trypsin/LysC mix (Promega, Madison, WI) using the SP3 beads protocol described in (Hughes et al. ...
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Photoreceptor disc incisures form as an adaptive mechanism ensuring the completion of disc enclosurebioRxiv - Cell Biology 2023Quote: ... Samples containing 20 µg of protein were mixed with 0.5 µg BSA (used as an internal standard in this analysis) and cleaved with 1 µg trypsin/LysC mix (Promega, Madison, WI) using the SP3 beads protocol described in (Hughes et al. ...