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Citations for Promega :
1351 - 1400 of 1564 citations for 2x SYBR Green qPCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... Orsay virus RNA1 level was quantified by qPCR using 1 μl of 1/5 diluted cDNA (GoTaq Promega A6001) and run on QuantStudio 3 Real Time PCR system (RNA1 qPCR primers GW194 and GW195)23.
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bioRxiv - Physiology 2023Quote: ... The obtained cDNA was used as the template for quantitative real-time PCR (qRT-PCR) using qPCR Mastermix (Promega). mRNAs expression levels were normalized to actin-5C mRNA used as the internal control The relative level for each gene was calculated using the 2-DDCt method (Hulf et al. ...
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bioRxiv - Cell Biology 2023Quote: ... The obtained cDNA was used as the template for quantitative real-time PCR (qRT-PCR) using qPCR Mastermix (Promega). mRNAs expression levels were normalized to actin-5C mRNA used as the internal control ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested overnight at 37 °C with Trypsin/Lys-C Mix (Promega, Charbonnières, France) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... proteins on the beads were digested by adding 400 ng trypsin/Lys-C mix (Promega) for 16 h at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Reaction mix was diluted twice with nuclease-free water (Nuclease-Free water, Cat# P119C, Promega) and 1 ul of amplified dsDNA was used for gene specific TaqMan Assay using the manufacture protocol for TaqMan™ Fast Advanced Master Mix (Cat# 4444964 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins on the beads were digested with 200 ng trypsin/Lys-C mix (Promega, V5072) for 16 h at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... 1x protease inhibitor mix (Benzamidine & PMSF)) supplemented with 7 U/ml DNase I (M610A, Promega) and lysed by Dounce homogenization and incubation on ice for 30 min ...
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bioRxiv - Microbiology 2022Quote: ... Neutravidin-bound proteins were digested on bead with 400 ng Trypsin/Lys-C mix (Promega) at 37 °C overnight with shaking ...
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bioRxiv - Systems Biology 2022Quote: ... 500 µg of proteins were digested with trypsin/Lys-C mix (V5071 or V5072, Promega) overnight at 37 °C with a 1:50 enzyme to protein ratio ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins bound to beads were digested with 1 μg of Trypsin/Lys-C mix (Promega) for 3 h at 37 °C and then supplemented with and additional amount of 150 ng Trypsin/Lys-C for overnight digestion on an end-over-end rotation apparatus at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative PCR was performed in a BioRad C1000 Touch thermocycler using GoTaq mix (Promega #A6001) [32] ...
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bioRxiv - Biochemistry 2022Quote: ... smegmatis Complex I (8 μg) was digested with MS-grade trypsin/Lys-C mix (Promega) at a 25:1 (w/w Complex I:protease ratio in 25 mM Tris-HCl ...
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bioRxiv - Plant Biology 2022Quote: ... the 40 µl in vitro translation reactions contained 20 µM 19 amino acid mix (Promega) and 0.45 µCi/µl of [35S]-methionine (Hartmann Analytics) ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were adjusted to 3mM EDTA and digested with 0.2μg Trypsin/LysC mix (Promega #V5073) for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... and then digested overnight with 300 ng of Trypsin/Lys-C Mix (Promega, Madison, WI). For on-bead digestion following SA pull-down ...
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bioRxiv - Systems Biology 2023Quote: ... 1 µL of enzyme mix (lysC & trypsin, 10 ng/µL in water, Promega, Cat. V5072) was dispensed into each well by using the pL-volume dispensing function of the cellenONE at 20°C and 85% humidity ...
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bioRxiv - Cancer Biology 2023Quote: ... we added 500 ng of trypsin/Lys-C Mix (CAT# V5072, Promega, Madison, WI, USA) to the beads soaked in 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and digestion with Trypsin and Lys-C (Trypsin/Lys-C Mix, Mass Spec Grade Promega) for 14 hours at 37° C.
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bioRxiv - Biochemistry 2023Quote: ... followed by 500 ng of trypsin/Lys-C Mix (CAT# V5072, Promega, Madison, WI, USA) and mixed gently at 37°C overnight to digest proteins ...
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bioRxiv - Cancer Biology 2023Quote: ... In this time the Fugene HD mix (8.6 μl Fugene HD (Promega, Madison, WI, USA) in 110 μl Opti-MEM was prepared and mixed thoroughly ...
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bioRxiv - Zoology 2023Quote: ... The mix contained 12.5 µL of GoTaq® G2 Hot Start Polymerase (Promega,, Wisconsin, USA), 1 µL of degenerated primers at 10 µM (HAN-L-F1 and HAN-L-R1 for primary PCR ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins on the beads were digested by adding 200 ng trypsin/Lys-C mix (Promega) at 37 °C overnight ...
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bioRxiv - Plant Biology 2021Quote: ... We added 300μl of Proteinase K Buffer 2X (200 mM Tris-HCl pH7.5; 100 mM NaCl; 20 mM EDTA; RNasin PROMEGA 20 U/μl; 0.04 mg/ml Proteinase K) and incubated for 1 h at 55 °C ...
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bioRxiv - Biochemistry 2021Quote: ... The dried resin was resuspended with 50 mM Tris pH 7.5 (2x resin volume) and 200 ng of trypsin was added (Promega; made up to 100 ng·μL-1 in H2O), the samples were digested overnight shaking at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... Samples were ground to fine powder using pestles on dry ice and then 100 μl 2x cell culture lysis buffer was added (Promega, E1531; with 150 mM Tris-Hcl pH7.5). 50 μl of leaf extract was mixed with 50 μl luciferase substrate (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR amplification was performed in 25 μl reactions using 5 μl X Green GoTaq Flexi Buffer (Promega), 1.5 μl MgCl2 (25mM ...
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bioRxiv - Biophysics 2020Quote: ... samples from homozygous HaloTag-TEV mice were incubated overnight with 5 μM HaloTag Oregon Green ligand (Promega) in Phosphate-buffered saline (PBS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Compound cytotoxicity in feline cells was assessed using the commercially available kit (CellTox Green Cytotoxicity Assay, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1ul of yeast lysate was used as template in a 10 µl GoTaq Green reaction (Promega M7123) with 0.25 µM of primers ...
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bioRxiv - Neuroscience 2021Quote: ... Grapefruit Integropectin cytotoxicity was assessed by CellTox™ Green Cytotoxicity Assay (Promega Corporation, Madison, WI 53711 USA). At the end of treatment ...
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bioRxiv - Cancer Biology 2020Quote: ... cell viability and cytotoxicity were measured with CellTiter-Glo®2.0 and CellTox™-Green assays (Promega) respectively according to the manufacturer’s instructions with a ClarioStar plate reader (BMG Labtech) ...
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bioRxiv - Microbiology 2021Quote: High molecular weight DNA was extracted using the Maxwell® RSC PureFood GMO and Authentication Kit as for qPCR (Promega). The high molecular weight DNA was sent to the Functional Genomic Center Zurich (FGCZ ...
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bioRxiv - Pathology 2021Quote: RT-qPCR (SARS-CoV-2 and MS2 viral genome detection) were performed with the GoTaq 1-step qRt-PCR kit (Promega) using 3.8µL of extracted RNA and 6.2µL of RT-qPCR mix that contains 250nM of each primer and 75nM of probe ...
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bioRxiv - Microbiology 2022Quote: ... standard curves were generated for each qPCR run from known copy numbers of a linearized pGEM-T Easy plasmid (Promega) containing the amplified PCR product to calculate the number of absolute copies in the samples.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... QRT-PCR reactions were performed by using the GoTaq®1-Step RT-qPCR System (Promega Scientific, Madison, WI, USA) with Bio-Rad CFX Connect (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2024Quote: The copy number of re-integrant strains was determined by extracting the genomic DNA and performing quantitative PCR (qPCR) using GoTaq polymerase (Promega) and a StepOnePlus real-time PCR machine (ThermoFisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... The isolated RNAs (20 ng) were reverse-transcribed and quantitative PCR (qPCR) were perform using GoTaq Probe 1-Step RT-PCR system (Promega) in a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: ... Gene expression was assessed by qPCR using primers listed in the supplemental material Table S6 and the GO Taq PCR kit (Promega). Gene expression was quantified using SYBR Green (Roche ...
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bioRxiv - Immunology 2023Quote: ... cDNA was generated from 20-100 ng of RNA using the GoTaq 2-step RT-qPCR System (Promega, cat# A6110). qPCR was performed with SYBR Green on a StepOnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Immunology 2022Quote: ... and proteins were subjected to overnight digestion with mass spec grade Trypsin/Lys-C mix (Promega). Following digestion ...
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bioRxiv - Plant Biology 2021Quote: ... 8 µL of a mix consisting of 2 µL deoxyribonucleotide triphosphate (dNTPs, Promega, Madison, Wisconsin, US), 4 µL 5x RT-buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... packaging mix was co-transfected with lentiviral vector in HEK293FT using FuGENE HD Transfection Reagent (Promega). Media containing transfection reagent was replaced with complete growth media after 24 hours ...
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bioRxiv - Biochemistry 2021Quote: ... and proteins were subjected to overnight digestion with Mass Spec Grade Trypsin/Lys-C mix (Promega). The beads were then pulled down and the solutions containing the digested peptides were desalted using AssayMap C18 cartridges mounted on an AssayMap Bravo liquid handling system (Agilent Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription was conducted using GoScriptTM Reverse Transcription Mix and Random Primers (A2800, Promega, WI, USA) according to the manual ...
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bioRxiv - Plant Biology 2023Quote: ... proteins were alkylated with iodoacetamide and digested with Trypsin/Lys-C Mix (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 1/20 of the cDNA was amplified by PCR using the GoTaq DNA polymerase mix (Promega) with specific antigenome primers (Appendix Table S1) ...
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bioRxiv - Systems Biology 2023Quote: ... and digested overnight at 37°C with a 2 μg Trypsin/Lys-C enzyme mix (Promega). The next day ...
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bioRxiv - Microbiology 2023Quote: ... then digested with 4 μL of a trypsin/Lys-C Mix (0.5 μg/uL) (Promega # V5071) using a two-step in-solution digestion overnight at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and subjected to in-gel digestion with trypsin/Lys-C Mix (V5071, Promega, Madison, WI, USA) at 37°C for 12 h ...