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Citations for Promega :
1451 - 1480 of 1480 citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... 2 x 105 U2OS cells were transfected with 2 µg of sgRNA and Cas9 expressing plasmid using Fugene HD reagent (Promega, Madison, WI, USA) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids with the arsC-1Fas and arsC-2Fas correct sequences were purified by miniprep (Wizard® Plus SV Minipreps, Promega, cat. n° A1360) and double digested with XhoI and HindIII (Thermo Scientific™ ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were transfected pCAG-Lgr6 or control plasmid (100 ng/ml or as noted) using the Fugene HD transfection reagent (Promega, Madison, WI, USA). Subsequent cell culturing and treatment is as noted for individual assays ...
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bioRxiv - Microbiology 2021Quote: In vitro transcribed (IVT) RNA standards were generated from the aforementioned plasmids using the T7 RiboMAX™ Express Large-Scale RNA Production System (Promega, Madison, WI). The concentration of each IVT RNA standard was measured by Nanodrop and the molecular weight was used to calculate the expected number of molecules per μL ...
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bioRxiv - Neuroscience 2020Quote: ... COS-7 cells were transiently transfected with 2μg plasmid (MuSK-HA, or GFP-Vangl2 alone, or in combination) using Fugene® 6 reagent (Promega, catalog no. E2311), according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2020Quote: ... Capped cRNAs were in vitro transcribed from linearized plasmid DNA templates using RiboMAX™ Large Scale RNA Production System (Promega, Madison, WI, USA). The maintenance of X ...
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bioRxiv - Neuroscience 2022Quote: ... The expression plasmids were constructed based on pCS2+ (see the section of Zebrafish) and were transiently transfected by using FuGENE HD (Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: These recombinant plasmids were co-transfected with the components of Dharmacon™ Trans-Lentiviral packaging kit into HEK293FT cells using FuGENE® HD (Promega Corp.) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... Germany)-labeled antisense riboprobe was synthesized from the plasmid containing the full or partial length open reading frame of the cDNAs using SP6 or T7 polymerase (Promega, Madison, WI, USA) after restriction enzyme digestion (New England Bio Labs ...
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bioRxiv - Microbiology 2023Quote: ... Standard calibration curves were performed using a 10-fold serial dilution (103 to 108 molecules) of standard pGEM-T Easy plasmids (Promega, Madison, WI, USA) containing target sequences from Escherichia coli K12 ...
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bioRxiv - Biochemistry 2023Quote: ... AGO1/2/3-/- cells were transfected with WT-AGO2-GFP or NES-AGO2-GFP plasmids with OptiMEM and FuGENE HD Transfection Reagent (Promega, Cat. No. E2311) with media change to full media at 24 hours post-transfection ...
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bioRxiv - Microbiology 2023Quote: ... As a qPCR standard the recombinant plasmid pBELX-1 (Bellanger et al., 2014) was extracted using the Wizard plus SV Miniprep DNA Purification System (Promega, Madison, WI, USA) according to the manufacturers’ instructions and linearized by restriction enzyme BamHI (Promega ...
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bioRxiv - Microbiology 2024Quote: HeLa cells were transfected with plasmids encoding ByeA or ByeAC197A according to the FuGENE transfection protocol (FuGENE® HD Transfection Reagent, Promega Cat. E2311) as advised by the manufacturer ...
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bioRxiv - Immunology 2024Quote: ... cells was transfected with 250ng of each of 2 pX330 constructs (containing Cas9 and 1 sgRNA) and 500 ng of the plasmid template with FuGene HD transfection reagent (Promega Corporation, Madison, WI) in the recommended conditions ...
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bioRxiv - Microbiology 2024Quote: ... an inhibition test was performed by mixing the soil DNA extracts with a control plasmid DNA (pGEM-T Easy Vector, Promega, Madison, WI, USA) or water ...
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bioRxiv - Biochemistry 2024Quote: ... Kc167 cells were seeded in 6-well plates and transfected (3 µg DNA per well) with the JHRE reporter or control (EGFP-pIEx-4) plasmids using the standard FuGENE-HD transfection protocol (Promega, Madison, WI, USA). About 48 h post-transfection ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Comparison of VUF15485-induced β-arrestin2 recruitment to human and mouse ACKR3 was also measured by NanoLuc complementation assay (NanoBiT, Promega Corporation, Madison, WI, USA) in HEK293T cells (Dixon et al. ...
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bioRxiv - Microbiology 2021Quote: ... Standard calibration was performed from a 10-fold serial dilution (103 to 108 molecules) of standard pGEM-T Easy plasmids (Promega, Madison, WI, United States) containing target sequences from Escherichia coli K12 ...
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bioRxiv - Synthetic Biology 2021Quote: ... a fairly large batch of transformed TOP10 cells was grown in 50 ml of LB and a plasmid DNA stock was isolated with the Wizard® Plus Midipreps system (Promega, catalog no. A7640). The poly(A ...
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bioRxiv - Neuroscience 2020Quote: ... The cells were co-transfected with 1 μg of the experimental firefly luciferase construct and 0.2 μg internal pRL control plasmid expressing Renilla luciferase driven by the CMV promoter (Promega, Madison, WI, USA; Cat# E2261). 24 hours after transfection ...
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bioRxiv - Cell Biology 2021Quote: HEK293 T-REx SNAPf-GLP-1R-SmBiT cells were seeded in 12-well plates and co-transfected with 0.1 μg β-arrestin-2 fused at the N-terminus to LgBiT (LgBiT-β-arrestin-2; Promega, plasmid no. CS1603B118) and either 0.1 μg mini-Gs-Venus or mVenus supplemented with 0.8 μg pcDNA 3.1.
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bioRxiv - Microbiology 2019Quote: ... purified amplicons were inserted into plasmids and ligation reactions (10 μ1) were set up as per manufacturer’s instructions (Promega pGEM-T Easy vector system). After ligation ...
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bioRxiv - Molecular Biology 2020Quote: ... purified amplicons from each single-plex TaqMan® qPCR were cloned into a plasmid vector using the pGEM®-T Easy Vector System I kit (Cat.# A1360, Promega, Walldorf, Germany) and One Shot® TOP10 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were then co-transfected with either 0.3 μg/well of Smad4 firefly luciferase reporter plasmid constructs (pLuc366 or pLuc207) or the control pGL3-Basic vector (Promega, San Luis Obispo, USA). The renilla luciferase plasmid was also co-transfected to correct for variations in transfection efficiency (45 ng/well) ...
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bioRxiv - Plant Biology 2021Quote: ... AccuPrep® Plasmid Mini Extraction Kit (Bioneer Corporation, Republic of Korea) and Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, USA) were used for plasmid and PCR product extraction and purification ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were transfected with 1 μg of DNA (1 μg of an L1-expressing plasmid or 0.5 μg of the L1-expressing plasmid and 0.5 μg of either a pCMV-3Tag-8-Barr control or ISG-expressing plasmid) using 3 µL of FuGENE HD transfection reagent (Promega, Madison, WI, United States) and 100 µL of Opti-MEM (Gibco ...
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bioRxiv - Immunology 2023Quote: ... stable HEK293 EBNA cell lines were generated using the sleeping beauty transposon system.91 Expression constructs were co-transfected with a transposase plasmid (10:1) into the HEK293 EBNA cells using FuGENE® HD transfection reagent (Promega GmbH, Madison, USA) in DMEM/F12 supplemented with 6% FBS ...
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bioRxiv - Cell Biology 2023Quote: Plasmids containing a promoter with binding sites for specific transcription factors to be measured driving firefly luciferase expression were co-transfected with a renilla luciferase reporter plasmid with a CMV promoter (Promega Corp, Madison, WI, USA) using TransIT reagent (0.25 µl/well ...
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bioRxiv - Biochemistry 2022Quote: ... cells reached 50-60% confluence and were transfected with 350 ng of ADGRL3 + 350 ng 22F Glosensor reporter plasmid (E2301; Promega – a gift from R. Lefkowitz lab) + 9 ng of β2-adrenergic receptor + 2.8 uL of transfection reagent Fugene 6 ...
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bioRxiv - Pathology 2022Quote: ... The infectious BSMV RNAs were transcribed in vitro from linearized plasmids γ-TaPDS, γ-TaCBS, γ-TaGSTU6, γ, α, and β (Petty et al., 1989) using the RiboMAX Large Scale RNA Production System-T7 kit (Promega, USA) and the Ribo m7G Cap Analog (Promega) ...