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Citations for Promega :
1351 - 1400 of 1480 citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: DNA constructs were transcribed and translated in vitro in pairs using equimolar plasmids in 70 μL of TNT® Quick Coupled Transcription/Translation System (Promega). RNaseOUT (0.5 ΜL ...
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bioRxiv - Cell Biology 2021Quote: ... Then a digoxigenin-labeled probe against sox846 was transcribed in vitro by using this linearised plasmid as a template and by following the instructions of a commercial in vitro Transcription System (Promega P1420). Templates for WT α-Tubulin-GFP (Addgene 56450) ...
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bioRxiv - Developmental Biology 2020Quote: ... The α-tub∷GFP (Chung et al., 2014) plasmid was injected at 5-10ng/μl after purification using the PureYield Midiprep kit (Promega, #A2492).
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bioRxiv - Cell Biology 2021Quote: ... HeLa cells were reverse-transfected at the day of seeding with 17µg of plasmid either coding for eGFP-RNF213 or eGFP-RNF213-ΔC using 51 µL of Fugene® HD (#E2311, Promega) for a ratio of 1:3 (µg cDNA / µL transfection reagent) ...
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bioRxiv - Molecular Biology 2021Quote: ... and cells with approximately 70-90% confluency in each plate were transfected with required plasmid(s) for each assay using Fugene 6 transfection reagent (Promega, USA) or 1 mg/ml PEI (polyethyleneimine).
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bioRxiv - Microbiology 2020Quote: ... The amplified signal peptide fragments were introduced into the plasmid pSUC2 (Prof. Sophien Kamoun, The Sainsbury Laboratory) using T4 DNA ligase (Promega, USA). The yeast invertase assay was used to validate the signal peptides (Jacobs et al ...
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bioRxiv - Microbiology 2020Quote: Plasmids encoding each vector were transfected into HEK293 cells seeded in 10 cm plates using Fugene 6™ (Promega, Madison, WI) or polyethyleneimine ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA fragments generated by PCR were cloned into the pGEM-T Easy plasmid vector using the pGEM®-T Easy Vector System (Promega) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... mRNA for each chimeric Lep protein was produced from plasmids using the RiboMAX RNA production system in accordance with the manufacturer’s instructions (Promega, Madison, WI). Lep proteins were then produced from mRNA by in vitro translation using rabbit reticulocyte lysate (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... of miR-148a putative binding sites reporter plasmids were constructed using the circMRPS35 and 3’-UTR of STX3 sequences in the psiCHECK2 vector (Promega, USA). For IRES activity analysis ...
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bioRxiv - Biophysics 2019Quote: ... MDA-MB-231 cells were stably transfected with the plasmids coding for 3-phosphoinositide-specific Akt-PH domain fused with CFP and the plasmids coding for Rac1 fused with YFP using FuGENE® HD transfection reagents (Promega). MCF-7 cells and MDA-MB-231 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Synthetic Biology 2019Quote: Measurements of luminescence intensity from the amber initiator plasmid system was performed as before15 using the NanoYGlo Luciferase Assay System kit (Promega, #N1110) with the following modifications ...
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bioRxiv - Cell Biology 2020Quote: Transfections were carried out with 1 μg of total DNA (500 ng for each construct or with empty expression plasmid) and 3 μl of FuGENE ® HD Transfection Reagent (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... 50 ng of an IFNβ promoter reporter plasmid (p125-luc) and 20 ng of a Renilla luciferase reporter (pRL-TK; Promega E2241) under control of a thymidine kinase promoter were co-transfected ...
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bioRxiv - Bioengineering 2021Quote: ... Plasmid DNA input was produced by midi prepping an overnight culture of 200 mL LB with the appropriate strain (Promega, A2492) and cleaned again using PCR cleanup (Promega ...
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bioRxiv - Immunology 2020Quote: EPC cells were seeded in 24-well plates overnight and cotransfected with various plasmids using FuGENE HD Transfection Reagent (Promega, #E2311) following the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2021Quote: ... YFP-NLS plasmid was digested by BamHI and HindIII and purified by Wizard® SV Gel and PCR Clean-Up System (Promega). Annealed oligonucleotides encoding UPF1’s NES with HindIII and BamHI overhangs at the end (UPF1-NES wt ...
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bioRxiv - Bioengineering 2022Quote: ... the mCherry coding sequence was PCR amplified from the plasmid pGFP-bait-MCS-NTR-mCherry using GoTaq® DNA Polymerase (Promega) with primers listed in Supplementary Table S3 ...
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bioRxiv - Molecular Biology 2022Quote: ... 250 ng of the pGL3-promoter vector (or the constructions derived from it) and 125 ng of the pCMV-β-gal plasmid (Promega) were used for transfection ...
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bioRxiv - Neuroscience 2022Quote: ... cRNAs were in vitro transcribed from linearized plasmid DNA templates using RiboMAXTM Large Scale RNA Production System (Promega, Madison, WI, USA). Xenopus oocytes were injected with 50 nl of RNase-free water containing 1.0 ng of cRNA (at a 1:1 molar ratio for heteromeric receptors ...
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bioRxiv - Molecular Biology 2023Quote: ... The PX459-DNMT1-gRNA plasmid was then used to transfect 293T cells using FuGENE HD Transfection Reagent (E2311, Promega, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... NLS-NSD2-mVenus WT and T1150A plasmids were transfected into 70 % confluent HEK293 SETD2 knockout cells using FuGENE® HD Transfection Reagent (Promega). NLS-mVenus empty vector was used as a negative control ...
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bioRxiv - Neuroscience 2022Quote: ... mP1/mP2 and the variants were purified from transformed bacteria grown in large-scale bacterial culture (50 mL Midiprep, PureYield™ Plasmid Midiprep System, Promega). The midipreps were made according to the manufacture’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... The total amount of transfected DNA was kept equal by supplementing with pCI plasmid DNA (Promega, Madison, United States of America). For electrophysiological recordings ...
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bioRxiv - Immunology 2022Quote: ... together with Jurkat cells (90,000 cells per well) stably transfected with mouse FcγRIV and NFAT-REluc2 expression plasmids (Promega, Madison, WI, USA). After incubation at 37°C for 6 hours ...
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bioRxiv - Microbiology 2022Quote: ... Transfection was achieved by adding 6 μg of plasmid DNA in 18 microliters of FuGENE® 6 Transfection Reagent (Promega # E2691); the obtained mixture was incubated in plain media for 20 minutes and then added to the pre-seeded culture dishes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Cells used for cAMP experiments were produced by PEI-transfection of HEK 293 cells with the commercially available plasmid pGloSensorTM-22F cAMP (Promega, GU174434). Those cells were additionally PEI-transfected with a plasmid containing the information for the adenosine A1 receptor under control of a CMV promoter ...
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bioRxiv - Molecular Biology 2023Quote: ... a mixture of the 3 Cas9-sgRNA plasmids (150ng/μl) was transfected into HEK293 cells at 70% confluency in fresh medium using FuGENE® HD Transfection Reagent (Promega). Two days after transfection ...
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bioRxiv - Microbiology 2023Quote: ... a plasmid serving as the template for homologous recombination of pCARD into the T4 genome was created by cloning the pCARD gene between 50 bp recombination overhang sequences (ordered from Twist Biosciences) in the pGEM-9Zf plasmid (Promega P2391). The recombination overhangs targeted the pCARD gene for insertion after the predicted promoter of the T4 IPI gene38 ...
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bioRxiv - Bioengineering 2023Quote: ... low passage cells were plated at a density of 15,000 cells per well in a 96-well plate and transfected at > 80% confluence with all-in-one PiggyBac plasmids containing CasRx or DjCas13d using ViaFect™ Transfection Reagent (E4981, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... cells were transfected at > 80% confluence with all-in-one PiggyBac plasmids containing different Cas13d orthologs using FuGENE® HD Transfection Reagent (E2311, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... low passage cells were plated at a density of 15,000 cells per well in a 96-well plate and transfected at > 80% confluence with all-in-one PiggyBac plasmids containing CasRx or DjCas13d using FuGENE® HD Transfection Reagent (E2311, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Mouse and human MIR122 promoter regions were amplified from genomic DNA and cloned in the pGL4.10[luc2] promoter-less luciferase plasmid (Promega, Walldorf, Germany,). Restriction sites for NheI ...
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bioRxiv - Immunology 2023Quote: ... cells were transfected with 12 µg of plasmid expressing the spike from various variants using Fugene-6 (Promega; catalog number: E2691) according to the manufacturer’s instructions and incubated at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... cells were transfected at > 80% confluence with all-in-one PiggyBac plasmids containing different Cas13d orthologs using FuGENE® HD Transfection Reagent (E2311, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Antisense probe was generated by cleavage of pSC-dmist plasmid with XbaI and in vitro transcribed with T3 polymerase (Promega P2083) using 1 µg DNA template according to the standard in vitro transcription protocol (see the full protocol at dx.doi.org/10.17504/protocols.io.ba4pigvn) ...
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bioRxiv - Biochemistry 2023Quote: ... U2OS PINK1 KO cells seeded at a density of 40,000 cells per 18mm glass coverslip in a 12 well plate were transiently transfected with 0.5 ug of plasmid DNA expressing WT-HA PINK1 under the control of a CMV weakened promoter using FuGene HD (Promega, E2311) at a ratio of 1:3 ...
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bioRxiv - Cell Biology 2023Quote: Activity of Hsf1 was determined by adding 50 µl of cell suspension (cells expressing the pSSA3HSE-lacZ plasmid) to 50 µl of β-Glo reagent (Promega) in a white 96-well plate ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultured in a 6-well plate and transfected with 10 µg of linearised plasmid expressing the SV40 Large T antigen using the FuGene Transfection Kit (Promega, #E2691) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: Notch reporter 4xcsl::H2B-mvenus plasmid(Nowotschin et al., 2013) was injected at 30ng/µl after purification using the PureYield Midiprep kit (Promega, #A2492). For antisense in situ hybridization probes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: A Human Embryonic Kidney 293 cell line (HEK 293; ATCC CRL-1573) stably transfected with luciferase-based pGlosensor-22F cAMP reporter plasmid (Glosensor, Promega Corp) has been described previously [GS2257] ...
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bioRxiv - Microbiology 2023Quote: A full-length CVB5 RNA was produced by in vitro transcription of an MluI-linearized plasmid using the T7 RiboMAX™ Express Large Scale RNA Production System (Promega). Then ...
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bioRxiv - Synthetic Biology 2023Quote: ... with a pLV-EF1a-IRES or pTwist-SFFV transgene expression vector and the viral packaging plasmids psPAX2 and pMD2.G using Fugene HD (Promega #E2312). Viral production was performed in 6-well tissue culture treated plates (Corning 3335) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected with pSpCas9(BB)-2A-Puro plasmid containing the sgRNA with Fugene HD transfection reagent (Promega Cat. No. E2311) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... The pCMV5D_HA-WDR44 plasmid was purchased from MRC PPU Reagents and Services (DU26855) and a pFN21A-Halo-KIAA0100 plasmid (which served as a PCR template) was purchased from Promega (FHC00016). GFP-Rab8 and GFP-Rab11 were kind gifts from Dr ...
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bioRxiv - Developmental Biology 2023Quote: Pou5f3-3xFLAG 12 and Nanog-HA (kind gift of Nadine Vastenhouw) expression plasmids were in-vitro translated using TnT® Quick Coupled Transcription/Translation Systems (Promega) according to the instructions ...
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bioRxiv - Microbiology 2024Quote: ... and simultaneously transfected with 0.4 μg of respective EGFP-Rab plasmids using FuGENE 6 transfection reagent (Cat. E269A, Promega, Madison, WI) per the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Plasmid pFL61 harbouring the construct RiSKC3-GFP under the control of the PGK promoter was purified from StellarTM bacteria using the PureYield™ Plasmid Miniprep System (Promega). The yeast transformation protocol was described by (50).
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bioRxiv - Cancer Biology 2021Quote: ... short tandem repeat analysis verified the identity of mouse (Bioassay Methods Group, National Institute of Standards and Technology) and human (Promega GenePrint 10 System) cells.
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bioRxiv - Microbiology 2019Quote: ... These stock solutions were then diluted to the indicated concentrations in sequencing-grade water and 10 ng commercial human DNA (Promega Corp cat#G3041) was added to all samples ...