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Citations for Promega :
951 - 1000 of 1480 citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The inserts and plasmid backbones were purified with Promega Wizard SV gel and PCR Cleanup System (Promega). Purified inserts and backbones were mixed in a molar 3:1 ratio ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 0.5 µg (HEK293A, HEK293T, HEK293AΔARRB1/B2) or 2 µg (HEK293AΔGs, HEKS293AΔGi) Glo-22F cAMP plasmid DNA (Promega) and 2 µg MOCK DNA (HEK293A ...
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bioRxiv - Synthetic Biology 2024Quote: ... and the lentiviral packaging plasmids pMD2.G (170ng) and pCMV-dR8.91(1.33μg) using 10μl of Fugene HD (Promega #E2312) according to manufacturer protocols ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells expressing the transporters were additionally transfected with a plasmid coding for NanoLuc (N1411, Promega, Madison, WI) and selected with 40 μg/ml hygromycin B (InvivoGen ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were transfected with 20 ng/well of psiCHECK-2 plasmid (psiCHECK-2 Vector (V0) (Promega, C8021) or let-7a-mi6 targeting six regions of the 3ill UTR ...
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bioRxiv - Molecular Biology 2023Quote: Plasmids were transfected into HepG2-NTCP cells using either polyethylenimine (PEI) or FuGENE HD Transfection Reagent (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... 16/32-cell embryos were co-injected with 5 pg of pRL-TK:renilla luciferase plasmid (Promega E2241) + 80 pg of the pGL4.23 luc2/miniP HumanPDX1 enhancer:firefly luciferase plasmid (wt or 6RFX motif mutant ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids containing rsbU or rsbT/rsbUY28I were subjected to PCR mutagenesis using GoTaq DNA polymerase mix (Promega) supplemented with 2mM additional MgCl2 ...
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bioRxiv - Genetics 2023Quote: ... which were introduced by amplification of the plasmid with the indicated mutation primers followed by DpnI (Promega) digestion ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids were sequenced to confirm the integrity of the construct and purified with a PureYield kit (Promega) for injection ...
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bioRxiv - Biochemistry 2024Quote: ... plasmids were performed with the transfection reagent FuGENE® HD (Promega, Ref: E2311, Promega GmbH, Walldorf, Germany) at a 2:1 FuGENE® HD:DNA ratio according to the manufacturer protocol ...
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bioRxiv - Microbiology 2024Quote: ... 20 µg of the plasmid was co-transfected with 2.5µg of pGL4.75 Renilla luciferase control vector (Promega) into DT40 AIDR/puro UNG-/- cells ...
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bioRxiv - Cancer Biology 2019Quote: ... rat or cynomolgus ICOS as target cells were co-incubated with ADCC reporter cells expressing human FcγRIIIa (V158; Promega) at a 5:1 ratio ...
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bioRxiv - Biochemistry 2020Quote: ... Human β2AR was fused at its N-terminus to the ACP- or Halo7-tag protein (NEB and Promega, respectively) at the cDNA level ...
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bioRxiv - Molecular Biology 2020Quote: ... The promoter area of human Rpl28 gene was cloned at HindIII/NcoI site of the pGL3-basic (Promega, E1751) to make pGL3-reporters ...
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bioRxiv - Genomics 2022Quote: Approximately 500-bp regions of DNA containing rs80282103 and rs11154336 were amplified from purified human genomic DNA (Promega, #G1521) by PCR using engineered restriction sites to allow directional cloning into the multiple cloning region of the pGL4.23[luc2/minP] luciferase reporter vector (Promega ...
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bioRxiv - Biophysics 2022Quote: ... pHalo-SMARCA4 expresses human SMARCA4 with HaloTag fused to the N-terminus under a CMVd1 promoter (Promega ORF FHC12075). pHalo-MED26 expresses human MED26 fused with a HaloTag at the N-terminus and was a kind gift from Joan Conaway’s lab ...
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bioRxiv - Cancer Biology 2022Quote: ... a 3kb region of the promoter containing HIC1 binding sites was amplified by PCR from human genomic DNA and cloned into the pGl4.26-luc vector (Promega); primers are listed in supplementary Table S1 ...
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bioRxiv - Molecular Biology 2022Quote: ... The human Gβ1 with a C-terminal 15-amino acid polypeptide linker was followed by a HiBiT (peptide 86, Promega), and the scFv16 was modified with an N-terminal GP67 signaling peptide and a C-terminal 8× histidine tag ...
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bioRxiv - Microbiology 2023Quote: ... and Sp-EVs towards human endothelial cells was accessed by the CellTiter-Blue® (CTB) Cell Viability Assay (Promega), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... After 30 minutes of incubation at 37°C in 5% CO2 we added 50,000 ADCC-RL cells (Jurkat cell line expressing luciferase gene under the control of the NFAT response element and stably expressing human FcγRIIIa V158; Promega) in 50 µl RPMI 1640 medium with 6% low-IgG serum (Promega) ...
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bioRxiv - Neuroscience 2023Quote: ... We amplified the regions from C57Bl/6 tail snip DNA or from human male genomic DNA (Promega catalog # G1471) using FastPhusion 2x Master Mix (Thermo Fisher catalog # F548L) ...
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bioRxiv - Cell Biology 2023Quote: ... TTBK2 KO cell lines were seeded on glass coverslips (Matrigel-coated for hPSCs) and the next day transfected with 0.5μg TTBK1-HaloTag® human ORF in pFN21A (FHC12512, Promega) or pglap1-TTBK2 (“GFP-TTBK2” ...
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bioRxiv - Genomics 2023Quote: ... Reference allele enhancer sequences for hZRS and hs737 were obtained via PCR cloning from human genomic DNA (Promega, G304A). Primers used for each enhancer sequence are outlined in Table S2 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cell surface levels of HiBiT-tagged human S1PR1 were monitored using a Nano Glo HiBiT Extracellular Detection System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: IDH1 and NNT promoter sequences were amplified from genomic DNA of human primary melanocytes by PCR and cloned into pGL4.12 (Promega). Mutagenesis of E-boxes was performed using a QuikChange Site-Directed Mutagenesis Kit (Stratagene) ...
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bioRxiv - Molecular Biology 2024Quote: ... and the equivalent region in the human (−207/+5) were cloned into pGL3-basic Luciferase reporter-vector (E1751, Promega) using the forward primer containing XhoI restriction site ...
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bioRxiv - Molecular Biology 2020Quote: ... These plasmids were then transfected in H1299 shPKM2 cells using FuGENE HD transfection reagent (Promega, Madison, WI, USA) and cultured in DMEM for 48 h (Fig ...
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bioRxiv - Microbiology 2019Quote: Plasmid pGEMt was used for the ligation of purified PCR products according to manufacturer’s protocol (Promega A1360, USA). The reaction mixture includes 5µl of buffer 2X ...
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bioRxiv - Molecular Biology 2021Quote: Cells were transfected with CRISPR–Cas9 and donor plasmids (Table 2) using FuGENE HD Transfection Reagent (Promega, #E2311) in a 6-well plate following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... 2.0 ug DNA of expression plasmids were mixed 150 μL Opti-MEM and transfected using FuGENE HD (Promega) at a DNA to FuGENE HD ratio of (1:3) ...
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bioRxiv - Cell Biology 2022Quote: For plasmid transfections cells were grown to 40-50% confluency and transfections were performed using FuGene6 (Promega, E2692) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... The total amount of DNA in the transfections was kept constant by supplementing with empty pCI plasmid (Promega). Plasmids encodings HCN1-4 ...
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bioRxiv - Biochemistry 2019Quote: ... HEK cells at a density of 3 million were transfected with 3.5μg each of CCR2 and the luciferase-based cAMP biosensor (pGloSensorTM-22F plasmid; Promega) plasmids ...
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bioRxiv - Cell Biology 2019Quote: ... Plasmids used for transfection were purified with Wizard Plus SV Minipreps DNA Purification System (Promega, Madison, WI, USA) or NucleoBond Xtra Midi (Macherey-Nagel ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were then transfected with 1500 ng pcDNA-FLAG-HA-sscGAS plasmid complexed with FuGene 6 reagent (Promega) according to manufacturer’s instructions or treated with FuGene 6 alone as a negative control ...
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bioRxiv - Immunology 2021Quote: ... The RARα open reading frame (NP_000955) was sub- cloned from the original plasmid (pFN21AE1591, Promega KK, Tokyo, Japan) into the pFC14K plasmid (Promega) ...
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bioRxiv - Neuroscience 2020Quote: ... pCAG-DCC:TDTOMATO and pCAG-DCCkanga:TDTOMATO plasmids (1 µg) were transfected into the plated U251 cells using FuGENE 6 (Promega) in Opti-MEM (Gibco ...
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bioRxiv - Bioengineering 2020Quote: ... Wizard Mini-Preps Plasmid DNA purification and SV Gel Clean-up kits were purchased from Promega (MyBio, Ireland). The O2-sensitive phosphorescent probe Pt-Glc was synthesized as described before [82] ...
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bioRxiv - Neuroscience 2020Quote: Transfected neuronal cultures with the NF-κB reporter Firefly Luciferase plasmid (Cat. N° E1980, Promega, Madison, WI, USA), were stimulated with NMDA/glycine for 60 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were transiently transfected with 0.1 ug of an individual plasmid with Fugene HD reagent (Promega, Madison, WI). 48 hours later ...
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bioRxiv - Systems Biology 2020Quote: Renilla control plasmid (3630 bp) was derived from another commercial vector pGL4.70 with the hRluc renilla gene (Promega) by insertion of a moderate-strength P transposase (pTran ...
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bioRxiv - Neuroscience 2020Quote: ... cells were plated in 24-well plates and transfected with 0.25 μg plasmid DNA and 1.25 μL FuGENE (Promega). Primary neuronal culture was prepared as described previously (40) ...
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bioRxiv - Microbiology 2019Quote: ... 48 hours later cells were transfected with a plasmid encoding HA of the FPV mutant using Fugene (Promega). 24 hours later cells were labelled for 2 hr with 200 µCi/ml [3H]-palmitic acid (9,10-3H(N) ...
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bioRxiv - Microbiology 2020Quote: ... The KAN PCR amplicon with compatible ends was ligated into the plasmid backbone using T4 DNA ligase (Promega). The resultant pEXP5/Kan vector was linearized using XbaI and Butters gp31FLAG was ligated into the plasmid for transformation into chemically competent BL21 cells ...
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bioRxiv - Microbiology 2021Quote: Plasmid pAMD375 was constructed using oligonucleotides JW10881 and JW10882 to amplify the NanoLuc luciferase gene from pNL1.1 (Promega). The resulting DNA fragment was cloned into pAMD001 (40 ...
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bioRxiv - Molecular Biology 2022Quote: RepEx-PCR-generated ~100x CAG repeats were inserted into a dual-luciferase reporter plasmid modified from pmirGLO (Promega) between firefly luciferase (FLuc ...
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bioRxiv - Synthetic Biology 2022Quote: ... with a pHR’SIN:CSW transgene expression vector and the viral packaging plasmids pCMVdR8.91 and pMD2.G using FuGENE HD (Promega, #E2312). Primary T cells were thawed the same day and after 24 h in culture ...
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bioRxiv - Microbiology 2022Quote: ... virus was removed and cells were transfected with 2 µg of plasmid DNA pUCSP-A24Rcd using FuGeneHD (Promega) following manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2022Quote: ... pH 8.0) with 2-5µg plasmids pre-digested by XcmI and 50 µg of herring sperm carrier DNA (Promega), then 700 µL of yeast transformation buffer (40% (w/v ...