Labshake search
Citations for Promega :
1001 - 1050 of 1480 citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2022Quote: ... pMD2.G and the lentiviral gRNA plasmid at a 3:1:5 mass ratio using FuGENE HD (Promega) in Opti-MEM (Thermo Fisher Scientific) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Luciferase activity was measured 24 hours after plasmids transduction by lysing the cells in the Passive Buffer (Promega) and combining equal volumes of cell lysates with the Bright-Glo™ Reagent (Promega) ...
-
bioRxiv - Microbiology 2021Quote: ... HepG2 cells were co-transfected with the firefly reporter vectors and the Renilla luciferase plasmid pRL-TK (Promega) as an internal control ...
-
bioRxiv - Microbiology 2021Quote: ... Plasmid DNA was isolated from each clone using Wizard® Plus SV Miniprep kit (Promega, Madison, WI, USA) and a total of 72 individual clones were sequenced with pJET1.2-F and pJET1.2-R primers at Eurofins Genomics Germany GmbH.
-
bioRxiv - Molecular Biology 2020Quote: ... plasmid pGL4.32 containing a firefly luciferase cassette under NF-κB response elements was obtained from Promega (Cat# E8491). For overexpression of ubiquitin ...
-
bioRxiv - Microbiology 2020Quote: ... duplicate wells were transfected with 1 µg HDM_Spike_RBD_B7-1 plasmids and 1 µg of Transfection Carrier DNA (Promega, E4881) using BioT reagent (Bioland Sci ...
-
bioRxiv - Microbiology 2021Quote: ... 293T cells were co-transfected with 1.5 mg of spike expression plasmids in pCDNA3 and 0.5 mg pmCherry-N1 using Fugene 6 and following the manufacturer’s instructions (Promega). Vero cells were treated with CellTracker™ Green CMFDA (5-chloromethylfluorescein diacetate ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... NanoBiT plasmids pBiT1.1-N_[TK/SmBiT] and pBiT2.1-N_[TK/LgBiT] were purchased from Promega (cat n° N2014).
-
bioRxiv - Molecular Biology 2019Quote: 1875 ng of circular plasmids were incubated at 37°C with 50 U of T3 RNA Polymerase (Promega) in a transcription buffer (40 mM Tris-HCl pH 7.9 ...
-
bioRxiv - Biochemistry 2019Quote: ... cells were co-transfected with firefly luciferase plasmid [pGL4.37 E364A for ARE(antioxidant response element):luciferase assays (Promega); pSGG containing the human Nrf2 3ʹ–UTR sequence (from Prof ...
-
bioRxiv - Bioengineering 2020Quote: ... This purified plasmid was used as a template for in vitro transcription with a Ribomax transcription kit (Promega) to generate RNA of ~1488nt ...
-
bioRxiv - Biochemistry 2019Quote: ... 0.2 μg pLEXm plasmid encoding WT or mutant KAP1 and 0.4 ng plasmid encoding Renilla luciferase using 1.5 μl of FuGENE 6 (Promega). Luciferase activity was measured 48 h post-transfection in cell lysates with the Dual Luciferase assay kit (Promega ...
-
bioRxiv - Molecular Biology 2020Quote: ... 300 ng CBS-VN (venus N-terminal) and 200 ng pmCherry-C1 plasmids) by using Fugene HD (Promega). At 24 h post-transfection ...
-
bioRxiv - Molecular Biology 2021Quote: ... 32μg SIV4+ (49)and with or without 1μg of pcDNA3.1 Vpx expression plasmid using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... were co-transfected with 0.5 μg of spike expression plasmids in pCDNA3 using Fugene 6 and following the manufacturer’s instructions (Promega). Cell-cell fusion was measured using an Incucyte and determined as the proportion of green area to total phase area ...
-
bioRxiv - Developmental Biology 2021Quote: ... A 2kb DNA fragment upstream of TSS of Snail1 promoter (ENSGAL00000008018) was cloned into pGL3 basic plasmid (Promega) using PWO polymerase (Roche ...
-
bioRxiv - Genomics 2020Quote: ... PCR oligonucleotides contained additional sequences complementary to Bgl II restriction endonuclease digested pGL3-promoter plasmid (Promega, Southampton, UK) for subsequently cloned using the InFusion HD Cloning Kit protocol (Takara Bio ...
-
bioRxiv - Molecular Biology 2021Quote: ... the entire open reading frame was inserted into the plasmid backbone from pFN24A HaloTag CMVd3 Flexi Vector (Promega) so that the ORF was under a CMV-d3 minimal expression promoter ...
-
bioRxiv - Microbiology 2021Quote: ... virus inoculum was removed and cells were then transfected with 2 µg of plasmid DNA using FuGeneHD (Promega) according to the manufacturer’s recommendations ...
-
bioRxiv - Neuroscience 2020Quote: 80,000 HEK293T cells were plated in 500μl HEK Media and 24 hrs later 500ng total plasmid DNA was transfected with 1.5μl FuGene HD (Promega) as follows ...
-
bioRxiv - Genetics 2020Quote: ... Cells were transfected with 130 ng of the assayed plasmid and 16 ng of pGL4.73 [hRluc/SV40] (Promega) containing Renilla luciferase to correct for transfection efficiency ...
-
bioRxiv - Developmental Biology 2022Quote: ... Cells were transfected with the Piggybac plasmid plus transposase at a 3:1 ratio using Fugene HD (Promega) and selected with G418 (300 µg/mL ...
-
bioRxiv - Cancer Biology 2022Quote: ... cells were co-transfected with 50 ng of the Renilla luciferase transfection control plasmid pRL-CMV (Promega #E2261) and 1 μg of either the ERα reporter plasmid XETL (here referred to as ERE-Luc ...
-
bioRxiv - Cancer Biology 2022Quote: ... 1mg of the plasmid was transfected to 293T cells (ATCC #CRL-11268) using FuGENE 6 Transfection Reagent (Promega) to produce the retroviruses ...
-
bioRxiv - Cell Biology 2022Quote: ... β-arrestin 2 fused at the N-terminus to LgBiT (LgBiT-β-arrestin 2; Promega, plasmid no. CS1603B118) was chosen as it has previously been used successfully with other class B GPCRs ...
-
bioRxiv - Cell Biology 2022Quote: ... and cloned upstream of the luciferase coding sequence of the pGL3-Basic plasmid (Promega, Madison, WI, USA, # E1751). The SAM68 expression plasmid was generated by insertion of the complete SAM68 coding sequence ...
-
bioRxiv - Developmental Biology 2022Quote: ... The amplicons of N1 (183 bp) and N2 (187 bp) were constructed into the pGL3-basic plasmid (Promega).
-
bioRxiv - Molecular Biology 2023Quote: ... RNA for microinjection was in vitro synthesized using as a template the plasmids previously linearized with BamHI (Promega) and transcribed using mMESSAGE mMACHINE SP6 kit (Invitrogen) ...
-
bioRxiv - Molecular Biology 2022Quote: ... the cells were transfected with 15 μg of an L1-expressing plasmid (pJM101/L1.3, pJM101/L1.3FLAG or pALAF008) using 45 μL of FuGENE HD (Promega) in 1,500 μL of Opti-MEM ...
-
bioRxiv - Molecular Biology 2022Quote: ... Digested plasmids and gBlock DNA fragments were purified by Wizard SV Gel and PCR clean-up system (Promega) and transformed in Stbl3 competent cells ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The two plasmids were transfected at a ratio of 20 ng:20 ng per well using ViaFect (Promega), employing a transfection agent to plasmid ratio of 3:1 μL:μg.
-
bioRxiv - Microbiology 2023Quote: Transfection of DNA plasmids into different mammalian cells was conducted using FuGENE® HD (Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... DF1 cells were transfected with the different RCAS retroviral plasmids using FuGENE 6 Transfection reagent (Promega, Cat. E2691), accordingly to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2022Quote: ... Plasmids were prepared by mini or maxi preps using commercially available kits (Qiagen, UK and Promega, UK, respectively) following manufacturers’ instructions ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were transfected with the following plasmids and amounts per well: 25 ng SRE-Luc reporter (E134A, Promega), 75 ng Gαi or Gαi QL in pcDNA3.1+ ...
-
bioRxiv - Biochemistry 2023Quote: ... In brief: full-length kinases were obtained as plasmids cloned in frame with a terminal NanoLuc fusion (Promega) as specified in Table S3 ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with plasmid DNA by the calcium phosphate or lipofection method using FuGene 6 (Promega, WI) (Yoshida et al. ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... The following amounts of DNA were used per well: 60 ng of pGloSensor-22F cAMP plasmid (Promega, E2301), 100 ng of receptor or empty vector (control ...
-
bioRxiv - Cell Biology 2023Quote: Immortalized DMDΔ52 myoblasts were co-transfected with 1.5ug of each plasmid containing the selected sgRNAs using ViafectTM (Promega) transfection reagent (1:5 ratio) ...
-
bioRxiv - Synthetic Biology 2024Quote: Pantropic VSV-G pseudotyped lentivirus was produced via transfection of Lenti-X 293T cells with a modified pHR’SIN:CSW transgene expression vector and the viral packaging plasmids pCMVdR8.91 and pMD2.G using Fugene HD (Promega #E2312). Primary T cells were thawed the same day ...
-
bioRxiv - Synthetic Biology 2024Quote: ... and the lentiviral packaging plasmids pMD2.G (170ng) and pCMV-dR8.91 (1.33μg) using 10ul of Fugene HD (Promega #E2312), according to manufacturer procedure ...
-
bioRxiv - Genomics 2023Quote: ... cells were transfected with 1µg L1 plasmid per well using 3µL FuGENE HD transfection reagent (Promega, Cat# E2311) and 97µL Opti-MEM (Life Technologies ...
-
bioRxiv - Neuroscience 2023Quote: ... The HdAd plasmid was linearized with PmeI and then transfected (Profection Mammalian Transfection System, Promega, Madison, WI, USA) into 116 producer cells ...
-
bioRxiv - Developmental Biology 2023Quote: ... The Carm1-reporter or its mutated reporters was co-transfected into 293T with the pRL-TK plasmid (Promega) expressing renilla for normalization ...
-
bioRxiv - Neuroscience 2024Quote: ... The WT and mutated 5’UTR sequences were ligated into a linearized reporter plasmid (psiCHECK-2 vector, Promega). The entire sequences of all the plasmids were validated by Sanger sequencing ...
-
bioRxiv - Cancer Biology 2023Quote: ... CDC20 and ANAPC4 knockouts were obtained with sgRNA plasmids transfected using FuGENE® HD Transfection Reagent (Promega, E2311), followed by selection using puromycin (Invitrogen ...
-
bioRxiv - Microbiology 2024Quote: ... Cells were transfected with 500 ng of each plasmid using 0.4 µl of Fugene 6 according to the manufacturer’s instructions (Promega) for 40 hours ...
-
bioRxiv - Microbiology 2024Quote: ... Cells were transfected with 500 ng of each plasmid using 1.5 µl of Fugene HD according to manufacturer’s instructions (Promega) for 40 hours ...
-
bioRxiv - Molecular Biology 2020Quote: ... African green monkey kidney (Vero) or human liver (HepG2) cells were performed via MTT assay using the CellTiter 96 Non-Radioactive Cell Proliferation (Promega) kit as previously described55 ...
-
bioRxiv - Molecular Biology 2021Quote: DRD1 Gs-mediated Gs-cAMP accumulation assays with HEK293T (ATCC CRL-11268) were performed using cells transiently expressing human DRD1 and the cAMP biosensor GloSensor-22F (Promega). Cells were seeded (20,000 cells/35 μL/well ...