Labshake search
Citations for Promega :
1201 - 1250 of 1558 citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: mRNA was synthesized in vitro with linearized template plasmids using the RiboMax Large Scale RNA Production System-T7 (Promega) supplemented with the Ribo m7G Cap Analog (Promega) ...
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bioRxiv - Cell Biology 2024Quote: 1μg of each plasmid or pcDNA3.1 empty vector was reverse transfected into 1x106 HEK293T cells using FUGENE HD (Promega) as per manufactures instructions ...
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bioRxiv - Microbiology 2023Quote: ... and plasmid extractions were done using the Wizard Plus SV Minipreps DNA Purification System according to the manufacturer’s protocol (Promega). The pk18mobsacB vector contains a kanamycin resistance cassette and the sacB gene ...
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bioRxiv - Molecular Biology 2023Quote: The human MEOX1 enhancer syntenic to the mouse Meox1 peak9/10 enhancer3 fragment along with the mouse Il1b peak5 and peak6 fragments were PCR amplified and cloned into the XhoI and HindIII sites in the Firefly luciferase plasmid pGL4.23 (Promega) using the Cold Fusion kit (System Biosciences Inc ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were transfected with three plasmids (Sox17 GFP, PX459-gRNA1, PX459-gRNA2) by incubation with FuGene HD (Promega, E2311) following a previously described protocol91 ...
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A systematic approach identifies p53-DREAM target genes associated with blood or brain abnormalitiesbioRxiv - Genetics 2023Quote: ... We used lipofectamine 2000 to transfect 106 NIH-3T3 MEFs with 3 μg of the luciferase plasmid with a WT DBS or its mutant counterpart and 30 ng of a renilla luciferase expression plasmid (pGL4.73, Promega) for normalization ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 million cells were seeded in a 10cm cell culture dish and were transiently transfected with 17 μg of plasmid cDNA (pcDNA 3.4 TOPO) and 53 μl Fugene HD (Promega) in 10 ml of HEK293 media ...
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bioRxiv - Molecular Biology 2023Quote: ... 500 ng of plasmid DNA encoding for SthCsm-complex and guide RNA was mixed with 1.5 μL of FuGENE HD reagent (Promega), incubated for 15 min ...
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bioRxiv - Cell Biology 2023Quote: The Foxo1/3/4 responsive luciferase reporter plasmid was constructed by inserting six FoxO REs (FoxO-responsive elements) into the pGL4.23 (Promega). The following sequence was used for the FoxO RE ...
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bioRxiv - Cell Biology 2023Quote: ... or 100-mm dishes were transfected with plasmids (2 μg /dish, 0.4 μg /well, or 10 μg /dish respectively) using FuGENE6 transfection reagent (Promega), according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... and 500 ng of lenti-viral transfer plasmids previously described along with 3 µl of Fugene 6 (E2692, Promega) transfection reagent ...
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bioRxiv - Cell Biology 2023Quote: DNA was prepared from bacterial cultures grown at 37°C using a Midiprep kit (PureYield Plasmid Midiprep System, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... pLenti-LgBiT-T2A-Blast was generated by subcloning the LgBiT coding sequence from the FRB-LgBiT plasmid (Promega N2016) into the lenti-dCas9-VP64-Blast plasmid.
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bioRxiv - Biochemistry 2023Quote: Cells stably expressing epitope-tagged NK1R were transiently transfected (Lipofectamine™ 3000 Transfection Reagent) with 1 μg of pGL4.29[luc2P/CRE/Hygro] or pGL4.30[luc2P/NFAT-RE/Hygro] plasmids (Promega Corp.), seeded in a white-walled 96-well flat clear bottom plate (Corning #3610) ...
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bioRxiv - Microbiology 2023Quote: ... Other plasmids used in this study include the pEGFP-N1 (Clonetech) and pRL-CMV vector (E226A) acquired from Promega. Cloning strategy for generating codon optimized SARS CoV-2 ORF7a in pCDNA-V5/His TOPO (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... and 250,000 cells were co-transfected with PB constructs (550 ng) and pBase plasmid (550 ng) using FuGENE HD transfection reagent (3.9 μl) (Promega), following the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2023Quote: ... Equilibrated cells were transfected with 0.8 μg of 5-HT1eR or 5-HT1FR and 8 μg of GloSensor plasmid (Promega), after mixing with 17.6 μl of PEI in OptiMEM (Gibco) ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 ng pCas9 mCherry Frame +1 and 100ng pCRISPaint HaloTag-Puro donor plasmids were transfected using ViaFect (Promega E498A). Three days post-transfection cells were sub-cultured into media supplemented with puromycin (0.8 µg/mL ...
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bioRxiv - Microbiology 2024Quote: ... These fragments were cloned into expression plasmids based on a pCI-neo mammalian expression vector (Promega, Madison, WI, U.S.A.), e.g ...
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bioRxiv - Cell Biology 2024Quote: ... Transfection of the different plasmids was made in a ratio 4:1:4 using lentiviral components pCMVR8,74 (Gag-Pol-Hiv1) and pMDG2 (VSV-G) vectors and the plasmid of interest respectively using FuGENE HD (Promega) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were seeded onto acid-washed coverslips at a density of 3 × 104 cells per coverslip for 24 h before transfection with 1μg of plasmid DNA using 3 μl Fugene HD transfection reagent (Promega). HaloTagged AP-2 σ2 was visualized by adding Janelia Fluor 646-HaloTag ligand (Promega) ...
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bioRxiv - Molecular Biology 2024Quote: ... The coding sequence of NanoLuc was amplified by PCR using the primers 5-NanoL and 3-NanoL from pNL1.1.CMV plasmid (Promega). The resulting fragment was digested with BamHI and NotI enzymes and cloned into pEGFP-N1 digested with the same enzymes ...
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bioRxiv - Cancer Biology 2024Quote: ... Transfection solutions were made at a lipid (µg) to plasmid (µL) ratio of 3:1 using FuGene 6 (Promega) as the transfection reagent ...
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bioRxiv - Molecular Biology 2024Quote: ... HeLa cells were co-transfected with 0.5 μg of “driver” plasmid (pTMO2F3) + 0.5 μg of pAluneoTet using 3 μL FuGENE® HD (Promega); the following day ...
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bioRxiv - Developmental Biology 2024Quote: ... the pCS2+empty and pCS2*HySp5 plasmids were incubated using the TNT Quick Coupled Transcription/Translation Systems (Promega L2080) and 1 µl was loaded on 10% SDS-PAGE.
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bioRxiv - Cell Biology 2024Quote: ... 125 ng of which was the reporter plasmid mixture (1:1 ratio of 8xGli-nanoluc/pGl3b to pGL4.53[luc2/PGK] (Promega)) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The plasmid DNA was purified from 4-mL overnight cultures by use of the Wizard Plus MiniPrep kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... and SERBP1 were amplified from human cDNA and cloned into the appropriate vectors (pACT or pBIND) provided by the CheckMate Mammalian Two-Hybrid kit (Promega E2440). The appropriate combination of edited pACT and pBIND vectors ...
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bioRxiv - Microbiology 2020Quote: ... or human IFN-β (1000 U/ml) and firefly luciferase activity was determined using the dual luciferase reporter assay system (Promega) on Cytation 3 (BioTek ...
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bioRxiv - Immunology 2022Quote: The constant regions of the human TCR genes were replaced with murine constant genes by overlapping PCRs as previously described,14 and the human/murine hybrid TCR genes were sub-cloned into the pGEM-4Z vector (Promega Corporation) for mRNA expression ...
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bioRxiv - Immunology 2022Quote: ... pneumoniae D39 or the isogenic mutants towards human epithelial cells was accessed using a CellTiter-Blue® (CTB) Cell Viability Assay (Promega), according to manufacturer instructions ...
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bioRxiv - Neuroscience 2024Quote: The short TMEM106B 3’ UTR and the long TMEM106B 3’ UTR were amplified from human genomic DNA (H1) and then cloned into the pmirGLO Dual-Luciferase Vector (Promega, E1330) using Gibson assembly ...
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bioRxiv - Cancer Biology 2024Quote: Genomic DNA was isolated from the 102 human FFPE colorectal tissue samples using the Maxwell® RSC Blood DNA Kit (Promega) on a Maxwell® 16 MDx (Promega) ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were blocked with PBS containing 5% skim milk and incubated with HRP-conjugated anti-human IgG1 antibodies (Promega, #W403B) overnight at 4 °C.
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bioRxiv - Microbiology 2023Quote: ... Standard curves were generated using purified genomic stocks (HSV-1 BAC, KSHV BAC, human genome Promega #G1471, mouse genome Promega #G3091), or purified plasmids (MHV68 ORF50) ...
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bioRxiv - Microbiology 2023Quote: ... Standard curves were generated using purified genomic stocks (HSV-1 BAC, KSHV BAC, human genome Promega #G1471, mouse genome Promega #G3091), or purified plasmids (MHV68 ORF50) ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK293TN cells were seeded in 6-well plates and transiently transfected with plasmid of choice using FuGene transfection reagent (Promega) and OptiMem (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were produced from pcDNA3-based plasmids at 30 °C for 90 min using the in vitro transcription & translation (IVTT) kit (Promega), following manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... HCT-OsTIR1 cells were co-transfected with these two constructs together with the PAF1-sgRNA expressing Cas9 plasmid using Fugene HD (Promega), and selected with 100 μg/ml Hygromycin B (Invivogen ...
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bioRxiv - Developmental Biology 2021Quote: ... The plasmids containing indicated CDS and tubP-GAL4 were co-transfected into the cells using FuGENE HD transfection reagent (E2311, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... 100 mm cell culture dishes were seeded with cells 24 hours prior to transfection and transfected the following day with the pCAGIG-Emx2 plasmid using FuGENE 6 transfection reagent (Promega) as per the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2021Quote: ... HEK-293T cells were co-transfected with corresponding pLKO.1 plamids and 3rd generation packaging lentiviral plasmids (pLP1, pLP2, pLP-VSVG) using FuGENE HD (Promega), according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2020Quote: Tle3 containing a flag tag in the C-terminus was cloned into the pTNT plasmid and transcription and translation was subsequently performed using the TNT Quick Coupled Transcription/Translation System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... and ‘TA cloned’ (after addition of 3’ adenine nucleotide overhangs) it into pGEM®-T-Easy plasmid vector (Promega, A1360). The derived plasmid was mutagenised (commercial service ...
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bioRxiv - Cancer Biology 2021Quote: ... 500 ng of lentiviral plasmid encoding doxycycline inducible transporters and 500 ng of each of the packaging plasmids were transfected into HEK 293T cells with FuGENE 6 transfection reagent (Promega). Cell supernatant was replaced 24 h after transfection and harvested after another 24 h ...
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bioRxiv - Neuroscience 2021Quote: Radioactive U1 and U7 snRNAs were generated by run-off transcription with T7 polymerase from linearized plasmid DNA templates in the presence of m7G cap analog (Promega) using the MEGAshortscript T7 kit (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were co-transfected with 2 µg of pWH464 (pSpCas9(BB)−2A-GFP (pX458)) expressing the corresponding targeting sgRNA and 1 µg of the corresponding donor oligo or plasmid using the Fugene6 (Promega). To generate miR-182Δ and miR-183Δ mESCs ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μL RRV-P3 plasmid or 3 μL of cDNA from rose tissue or nuclease-free water (Promega, Madison, WI). The final volume of the LAMP reaction was 25 μL.
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bioRxiv - Cell Biology 2020Quote: ... 40% confluent HAP 1 WT cells (seeded in a 10-cm dish) were co-transfected with the two plasmids described above (5 µg each) using 30 µl Fugene6 transfection reagent (Promega) in OptiMEM medium ...
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: ... the gRNA/Cas9 plasmid as well as the plasmid carrying homology arms and mAID-mClover neomycin cassette were transfected into HCT116 CMV-OsTIR1 cells using FuGene HD (Promega). After 6 days selection with 700 µg/ml G418 the cells were FACS-sorted for mClover expression and seeded into 96 well plates to grow single clones ...