Labshake search
Citations for Promega :
1251 - 1300 of 1480 citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: To generate the pGl3-STK38-P luciferase reporter,1.2 kb of DNA upstream the STK38 TTS promoter region with ZNF611 binding motif was amplified by polymerase chain reaction (PCR) and cloned into the Firefly luciferase reporter plasmid pGL3-Basic (Promega) using KPN1 and XHO1 restriction sites.0.7 kb of DNA upstream the STK38 TTS promoter region without ZNF611 binding motif was cloned into pGL3-Basic using same restriction sites to generate the pGl3-STK38-ΔP luciferase reporter ...
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bioRxiv - Genomics 2021Quote: 293T cells were co-transfected with plasmid DNAs of a retroviral vector and the helper vectors using FuGENE HD (Promega). Medium was collected 24 ...
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bioRxiv - Microbiology 2020Quote: ... HEK293T cells were seeded in T75 tissue culture flasks to be ∼80% confluent the following day and were transfected with the plasmid of interest using Fugene 6 (Promega). After ∼18 h the transfection media was removed and the cells were infected with VSVΔG∗rLuc at a multiplicity of infection of ∼0.07 for 1 h at 37°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... with restriction enzyme overhangs were ordered from Genewiz and cloned into pGL3-basic plasmid upstream of the firefly reporter gene (E1751, Promega). Minipreps were prepared with QIAprep Spin Miniprep kit (Qiagen) ...
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bioRxiv - Molecular Biology 2021Quote: ... The NF-κB reporter plasmid containing five copies of an NF-κB response element fused to the firefly luciferase gene and TK-renilla control plasmid were obtained from Promega. The IFIT1 (ISG56 ...
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bioRxiv - Immunology 2020Quote: ... Plasmid transfections of IFITM1 3’ UTR reporter plasmids (500 ng per single well of a 6-well plate) were performed using the FuGENE 6 (Promega), according to manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... cells were transfected with a DNA mixture containing 50 ng of firefly luciferase reporter plasmid (Promega pGL4.31 [luc2P/Gal4UAS/Hygro]), 5 ng of Renilla luciferase plasmid (Promega pRL-CMV) ...
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bioRxiv - Microbiology 2021Quote: ... HEK293T cells were transiently transfected with plasmid DNA pHIV-1 NL4·3Δenv-Luc and Spike-Δ19-D614G by using Profection mammalian transfection kit (Promega Inc) following the instructions in the kit manual ...
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bioRxiv - Developmental Biology 2021Quote: ... The BRE response element was inserted upstream of the minimal promotor region of plasmid pGL4.26 luc2/minP/Hygro (Promega E8441) that also encodes the luciferase reporter gene luc2 ...
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bioRxiv - Genomics 2021Quote: ... were transfected at 80-90% confluence with lentivirus helper plasmids pVSV-G and psPAX2 in along with the constructed lentiviral transfer vector using FuGENE HD (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins used for EMSA experiments were synthesized in-vitro from pTNT plasmids using TNT T7 Quick Coupled Transcription/Translation System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... recombinant viral Tha-eGFP and Th2P-4M-eGFP constructs were used which were generated by cloning sequences of eGFP (recovered from pEGFP-C1 plasmid, Promega) into the genetic sequence of Tha (35 ...
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bioRxiv - Immunology 2022Quote: ... and a non-target control (NT-Ctrl) along with the packaging plasmids (pHCMV-G, and pHCMV-HIV-1) (44) using FUGENE-HD transfection reagent (Promega). The mouse Drp1-targeted shRNA plasmid with the sense sequence of GGCAATTGAGCTAGCTATA ...
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bioRxiv - Microbiology 2022Quote: ... standard curves were generated for each qPCR run from known copy numbers of a linearized pGEM-T Easy plasmid (Promega) containing the amplified PCR product to calculate the number of absolute copies in the samples.
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bioRxiv - Microbiology 2022Quote: ... CERTL destined for expression using in vitro transcription/translation system from wheat germ extract was cloned into the plasmid pF3A WG BYDV (Promega) with an N- terminal myc epitope tag ...
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bioRxiv - Cell Biology 2022Quote: ... the DLD1-OsTIR cells plated in a 3.5 cm dish was transfected with the mAID-donor and guide RNA (gRNA) plasmid constructs using ViaFect (Promega, #E4981) (Mali et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... The wells were co-transfected with different PFKFB3-HRE luciferase constructs along with pRL-TK renilla luciferase plasmid (Promega, E2231). 12hrs post-transfection ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μg of psPAX2 packing plasmid and 2 μg of pM2G envelope plasmid were transfected into HEK293 Lenti-X cells using 30 μL of Fugene-HD (Promega) in antibiotic-free DMEM ...
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bioRxiv - Developmental Biology 2022Quote: HEK293 cells were cultured as described (Poliakov et al., 2008) and transfected with 1 µg of the appropriate plasmid using FuGENE HD transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... S2-VP10 cells were co-transfected with 0.5 μg of each luciferase reporter plasmid and 1 ng of pRL-SV40 (Promega, E2231) the day after plating (3.45 × 104 cells/well in a 24-well plate ...
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bioRxiv - Physiology 2022Quote: ... and 0.5 μg of reference plasmid pRL-TK carrying the Renilla luciferase gene under the control of the simian virus 40 enhancer and promoter (Promega). Lipofectamine 3000 reagent (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... the indicated plasmids were added to a total of 2.0 ug DNA (with pLEX307 RFP as the filler plasmid) in 125 uL Opti-MEM and transfected using FuGENE HD (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... at 72° C for 10 min to add 3’-A overhangs for T/A cloning with pGEM-T Easy plasmid (Promega). A 610 bp DNA fragment upstream of flgV was amplified using PCR primers 85 and 86 ...
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bioRxiv - Molecular Biology 2022Quote: ... VA or Mutated (M)-sequences with the corresponding complementary strands (Supplementary Table S1) and then cloned using SmaI restriction site upstream the basal SV40 promoter into pGL3-promoter vector plasmid (Promega), in the same strand (coding or template ...
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bioRxiv - Synthetic Biology 2022Quote: ... and plasmids were extracted from individual cultures outgrown in LB/ampicillin (50 μg/mL) using the PURE Yield Plasmid Miniprep kit (Promega). Individual clones were screened and confirmed by Sanger sequencing at Macrogen-Europe B.V ...
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bioRxiv - Plant Biology 2022Quote: ... Each of the target site sequences was introduced into Arabidopsis U6 promoter-gRNA cassette cloned into pGEM-T Easy plasmid (Promega) using two-step overlapping PCR (Figure S1) ...
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bioRxiv - Neuroscience 2022Quote: ... Flag-tagged STAU1 or STAU1RBDΔ3 or Flag plasmids with the wildtype or mutant 5’UTR-MTOR-LUC or luciferase empty vector constructs and Renilla luciferase plasmid (pRL-SV40 vector, Promega) according to an experimental set-up ...
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bioRxiv - Microbiology 2023Quote: ... HEK293T cells were transiently transfected with plasmid DNA pHIV-1 NL4·3Δenv-Luc and Spike-Δ19-D614G by using profection mammalian transfection kit (Promega Inc.). After 48 hours ...
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bioRxiv - Neuroscience 2022Quote: ... the cDNA fragments corresponding to the coding region or 3′-UTR of mouse Inka2 cDNA were subcloned into a pGEM-3Z plasmid vector (Promega) for in vitro transcription ...
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bioRxiv - Microbiology 2022Quote: ... and a spike-expressing plasmid (pCAGGS-SARS-CoV-2-spike) were co-transfected into HEK293T cells using Fugene HD transfection reagentia (Promega) according to the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2022Quote: ... virus inoculum was removed and cells were then transfected with 2 μg of plasmid pm-cherry-A4L (kindly provided by Wen Chang) using FuGeneHD (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... Ectopic Flag-tagged Galectin 8 or HA-tagged LILRB4 plasmid were transfected into the Mc38/B16/HEK294 cells with Fugene (Promega). A blank vector control was applied ...
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bioRxiv - Biochemistry 2022Quote: To generate TRIM7 lentiviruses pSMPP-hTRIM7 constructs were co-transfected into HEK293T cells with pMDG2 and pCRV-GagPol plasmids using FuGENE6 (Promega) reagent and incubated for 3 days ...
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bioRxiv - Molecular Biology 2022Quote: ... The ObLiGaRe-TLR construct was co-transfected with Zinc Finger Nuclease (ZFN)-AAVS1 plasmid in a 1:3 ratio using FuGENE transfection reagent (Promega) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: HEK293T cells with the RAI13103insC mutation were generated by introducing the plasmid expressing Cas9 and sgRNA with single-stranded oligodeoxynucleotides (Supplementary Sequence 2) by FuGENE HD (Promega). The Cas9-expression plasmid with the sgRNA sequence for generating the RAI13103insC mutation and the GFP/puromycin expression plasmid (70 ng of each plasmid ...
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bioRxiv - Microbiology 2022Quote: ... and the lentiviral packaging plasmids pVSV-G, pDVPR, and pAdvant (gifts from Jan Carette, Stanford University) using FuGENE HD transfection reagent (Promega) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... These fragments were cloned into the expression plasmids based on a pCI-neo mammalian expression vector (Promega, Madison, WI, USA), e.g ...
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bioRxiv - Cell Biology 2023Quote: ... was obtained through transfecting HEK293T cells with these plasmids in combination with a second generation CMV lentiviral packaging system and FuGENE transfection agent (E2311, Promega).
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bioRxiv - Cell Biology 2023Quote: ... cells were plated on three 10 cm tissue culture dishes per condition and transfected 24 h before lysis using FuGENE 6 (6-12 μg total plasmid DNA; Promega). Published protocol can be found on Protocols.io (dx.doi.org/10.17504/protocols.io.kxygx3zeog8j/v1).
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bioRxiv - Cancer Biology 2023Quote: ... and 500 ng of each of the packaging plasmids were transfected into 293T cells using FuGENE 6 transfection reagent (Promega). The viral media was exchanged after 24 h ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells in a 6-cm dish were transiently transfected with 1 μg of pGloSensor-22F cAMP plasmid (Promega, USA) and 1 μg of human wild-type or mutated A2AR or A2BR overexpression plasmid using polyethyleneglycol (6 μl ...
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bioRxiv - Molecular Biology 2023Quote: ... and 50 ng of the plasmid encoding for the GFP-stop-Luc reporter were mixed with 1.1 μ FuGENE HD reagent (Promega), incubated for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... a pPolI-Firefly plasmid encoding the Firefly luciferase sequence in negative polarity flanked by the 5’ and 3’ non-coding regions of the IAV NS segment was used and the pTK-Renilla plasmid (Promega) was used as an internal control ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the coding sequence of HIV-1 Nef (SF2 variant) was amplified by PCR and inserted into the plasmid pNLF-1-C (Promega) for expression of NanoLuc at the Nef C-terminus ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... was co-transfected at a 1:5 ratio with either the HaloTag-ubiquitin plasmid (expressing Ub-Halo) or the HaloTag control (Promega). Transfected cells were incubated for 20 h at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... Shigella conjugants that grew at 30°C and were Cb60 resistant were screened by PCR for the presence of lpxE and the large virulence plasmid using GoTaq (Promega) and primer sets lpxE-F/lpxE-R (lpxE ...
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bioRxiv - Molecular Biology 2023Quote: ... Flp-In T-REX HeLa cells were transfected with 1.8 μg of pOGG4 plasmid and 200 ng of pcDNA5/FRT/TO plasmid encoding either a GFP-BICD2N-MTS or a GFP-MTS cassette using FuGENE HD (Promega). Cells with a stably integrated transgene were selected with 150 μg/mL hygromycin over 4-5 days ...
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bioRxiv - Cancer Biology 2023Quote: ... We co-transfected cells with the Tol2 GIV transposon and a Tol2 transposase (Vector Builder) at a 3:1 ratio of micrograms of plasmid DNA using Fugene 6 (Promega) according to the manufacturer’s directions ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR was used to amplify the coding regions of the steroid receptor genes and to linearize the expression plasmids pHTN HaloTag CMV Neo or pFLN-1 NanoLuc (Promega #N1811 ...
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bioRxiv - Immunology 2023Quote: ... Retrovirus was generated by transfecting the Plat-E packaging cell line with 10 µg plasmid DNA and 30 µl FuGene HD transfection reagent (Promega). Viral supernatants were collected from transfected Plat-E cells at 40 ...