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Citations for Promega :
1251 - 1300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Deletion mutants of Caskin2 and Caskin2-4A were generated by site-directed mutagenesis with the PCR-based overlap extension method using Pfu DNA polymerase (Promega), and fragments containing the different mutations were exchanged with corresponding fragments in the pUC19-CASKIN2-GFP plasmid ...
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bioRxiv - Cell Biology 2024Quote: ... biological replicates (n=7) were digested with Lys-C (Alpha Laboratories) and trypsin (Promega) on beads as previously described (Hubner et al. ...
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bioRxiv - Cell Biology 2024Quote: ... images produced from HeLa cells seeded on glass-bottom Mattek dishes (P35GC-1.5-14-C) and transfected with TFG-mEGFP-FLAG at 1 µg/µL using Fugene (Promega, cat # PRE2311) for 24 hours ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected with TFG-mEGFP-FLAG at 1 µg/µL using Fugene (Promega, cat # PRE2311) and left to express for 24 hours ...
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Loss of intermicrovillar adhesion impairs basolateral junctional complexes in transporting epitheliabioRxiv - Cell Biology 2024Quote: A validated CDHR2 KO CL4 cell clonal population was transfected with pHALO-N3-CDHR2 using FuGENE 6 (Promega #E2691) at a FuGENE:DNA (μL:μg ...
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bioRxiv - Cell Biology 2024Quote: ... in a 96-well-plate using a blue filter of the GloMax Discover Microplate Reader (Promega). Obtained values were corrected by subtracting background fluorescence value (i.e. ...
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bioRxiv - Cell Biology 2024Quote: ... The pool was quantified by Fluorometry using the QuantiFluor ONE dsDNA System (Cat# E4871, Promega, Madison, WI, USA) and sequenced Single-Reads 76 bases (in addition ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were digested by incubation with sequencing-grade modified trypsin (1/100, w/w; Promega, Madison, Wisconsin) for 12 h at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... The pool was quantified by Fluorometry using the QuantiFluor ONE dsDNA System (Cat# E4871, Promega, Madison, WI, USA) and sequenced Single-Reads 76 bases (in addition ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were digested overnight with sequencing-grade modified trypsin (Promega). Peptides were desalted with Sep-Pak C18 well plate (Waters) ...
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bioRxiv - Cell Biology 2024Quote: ... The following primers were used to amplify PCR products which were used to generate in situ hybridization probes with T7 RNA polymerase (Promega) and Digoxigenin-UTP (DIG RNA Labeling Mix ...
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bioRxiv - Cell Biology 2024Quote: Luciferase activity was measured using the Dual-Luciferase Reporter Assay System (Promega, USA) and measured using Varioskan Flash multimode reader (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... then were inserted into the p2T vector cut with NheI (Promega) and SmaI (Promega) ...
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bioRxiv - Cell Biology 2024Quote: ... reverse transcription primers and dNTPs (both Promega). Single cell sequencing was performed according to the Sort-seq method13.
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bioRxiv - Cell Biology 2024Quote: ... and SmaI (Promega). Organoid lines were generated by co-electroporation of 5 µg of the respective FUCCI ...
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bioRxiv - Cell Biology 2024Quote: ... digested with trypsin (Promega, V5111), and peptides analyzed using Q-Exactive HF-X as described52.
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bioRxiv - Cell Biology 2024Quote: ... Protein-bound magnetic beads were washed three times with 200 µl of 80% ethanol and reconstituted in 50 mM TEAB and digested with trypsin (Promega, V5111) at a 1:50 enzyme-to-substrate ratio for 16 h at 37 °C with constant shaking (1000 rpm) ...
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Phospholipase C β4 promotes RANKL-dependent osteoclastogenesis by interacting with MKK3 and p38 MAPKbioRxiv - Cell Biology 2024Quote: ... and envelop plasmid (VSVG) using FuGENE HD transfection reagent (Promega, Madison, WI, USA). The supernatant containing the constructed lentiviruses was collected after 24–48 h of transfection ...
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bioRxiv - Cell Biology 2024Quote: ... After digestion with trypsin (mass spec grade, Promega), peptides were extracted with acetonitrile ...
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Phospholipase C β4 promotes RANKL-dependent osteoclastogenesis by interacting with MKK3 and p38 MAPKbioRxiv - Cell Biology 2024Quote: ... the existing media was removed and replaced with 100 µL of medium containing 20 µL of CellTiter 96 AQueous One solution (Promega). The cells were then incubated for 4 h at 37°C in a humified atmosphere with 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were digested by adding Sequencing Grade Modified Trypsin (Promega; 400 µg/ml in 25 mM AmBic ...
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bioRxiv - Cell Biology 2024Quote: ... Quadruplicates were analyzed and standardized with luciferase (Promega) or stably expressed housekeeping genes (18S ...
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bioRxiv - Cell Biology 2024Quote: ... was used with random primers (Promega, Charbonniéres, France) to synthesize cDNA from 1μg of total RNA ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with 2 μg of bacmid DNA using Fugene HD transfection reagent (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... alkylated with 10 mM iodoacetamide and subject to sequential protein digestion with Lys-C and trypsin (Promega). Digestion was halted with formic acid ...
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bioRxiv - Developmental Biology 2024Quote: ... and secondary antibodies (anti-mouse HRP conjugate (Promega, W4028) and anti-rabbit HRP conjugate (Promega ...
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bioRxiv - Developmental Biology 2024Quote: ... and anti-rabbit HRP conjugate (Promega, W4018)).
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bioRxiv - Developmental Biology 2024Quote: ... Total peptide concentration was adjusted to 0.15 µg/µL according to the absorbance at 280 nm against a Mass Spec-Compatible Human Protein Extract/Digest calibration curve (Promega), using the Thermo Nanodrop 1000 ND-1000 spectrophotometer (Thermo Fisher).
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bioRxiv - Cell Biology 2024Quote: ... Fugene 6 (Promega) or Fugene 4K (Promega ...
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bioRxiv - Cell Biology 2024Quote: ... or Fugene 4K (Promega) were used ...
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bioRxiv - Cell Biology 2024Quote: ... Stag2 and Pds5b variants were first expressed by ‘cold’ coupled in vitro transcription- translation (IVTT; TNT reticulocyte lysate, Promega). IVTT aliquots (3 µl ...
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bioRxiv - Cell Biology 2024Quote: ... Maxwell® RSC (Promega Corp.), with the simplyRNA Tissue Kit (AS1340 ...
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bioRxiv - Cell Biology 2024Quote: ... qPCR was performed using GoTaq qPCR Master Mix (Promega, Madison, WI, USA) with StepOne Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... HIS-PLK1 used in ADP-Glo was from Promega (V2841). HIS-PLK1 used in Fig ...
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bioRxiv - Cell Biology 2024Quote: ... gel bands were processed by following a standard protocol 87 with trypsin (Promega) digestion at a 10-ng/μL concentration overnight ...
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bioRxiv - Cell Biology 2024Quote: ... was measured in a 96-well-plate using a blue filter of the GloMax Discover Microplate Reader (Promega).
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bioRxiv - Cell Biology 2024Quote: ... fluorescence intensity was measured using a fluorescence plate reader with an excitation wavelength of 485 nm and an emission wavelength of 538 nm (GloMax® Discover Microplate Reader, Promega). The relative percentage of intracellular ROS was normalized respect to untreated cells (control).
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bioRxiv - Cell Biology 2024Quote: ... S3A and S8D was purchased from Promega (V4020). HIS-PLK1 used in ADP-Glo was from Promega (V2841) ...
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bioRxiv - Developmental Biology 2024Quote: pCS2+ expression vectors carrying membrane-localized fluorescent CAAX-GFP (mGFP) or Utrophin-RFP (utr-RFP) were linearized using restriction enzymes (Promega) for two hours at 37°C and precipitated at -20°C overnight in 70% ethanol ...
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bioRxiv - Biochemistry 2024Quote: ... and yeast (Y) (Promega, MS Compatible Yeast Protein Extract ...
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bioRxiv - Developmental Biology 2024Quote: ... Reverse transcription from 1 μg of total RNA was performed with the ImPromII (Promega) kit ...
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bioRxiv - Developmental Biology 2024Quote: ... Amplicons were separated by electrophoresis and the corresponding bands were purified with the Wizard SV Gel and PCR Clean-Up System (Promega) kit ...
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bioRxiv - Developmental Biology 2024Quote: ... was amplified by PCR using tnnc1a-CDS primers (Table 1) and cloned into the pGEM®-T Easy Vector Systems (Promega, catalog #: A1360). A tnnc1a cDNA clone was confirmed by sequencing and used to make digoxigenin-labeled riboprobes with the Ambion™ MAXIscript™ SP6/T7 In Vitro Transcription Kit (Thermofisher ...
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bioRxiv - Developmental Biology 2024Quote: ... ligated in pGEM-T Easy Vector (Promega, Madison, WI, USA), and cloned in DH-5α E ...
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bioRxiv - Developmental Biology 2024Quote: ... Kanamycin-resistant colonies were selected and cultured o/n purified with Plasmid Miniprep system (Promega) and verified by sequencing (Microsynth ...
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bioRxiv - Developmental Biology 2024Quote: ... and cloned into pGEM-T Easy vector using pGEM-T Easy Vector System I (Promega; CAT A1360). Vectors were transformed into Escherichia coli using the heat-shock method ...
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bioRxiv - Cell Biology 2024Quote: ... The protein pellets were resuspended in 50 μl 100 mM TEAB and digested with 200 ng trypsin (Promega sequencing grade) overnight at 37°C.
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bioRxiv - Cell Biology 2024Quote: ... anti-mouse IgG (H+L) HRP conjugate (Promega #W4021, 1:10000), anti-rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2024Quote: ... anti-rabbit IgG (H+L) HRP conjugate (Promega #W4011, 1:10000).
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bioRxiv - Cell Biology 2024Quote: ... and lentiviral-containing supernatant was tested for mycoplasma contamination and then concentrated in 50% polyethylene glycol (PEG) 8000 (Promega V3011) for at least 72h at 4°C ...