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Citations for Promega :
1101 - 1150 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: Luciferase assays were performed using reporter lysis buffer (Catalog #E3971, Promega, U.S.A) and luciferase assay reagent according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The reaction was carried out using RiboMAX™ Large Scale RNA Production System (T7) (Catalog #P1300, Promega, U.S.A) and biotin-11-UTP (Catalog #AM8451 ...
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... Orsay Virus RNA1 level was quantified by qPCR using 1 μl of 1/5 diluted cDNA (GoTaq Promega A6001) and run on QuantStudio 3 Real Time PCR system ...
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... 1 μl of the supernatant was used to make cDNA (Promega GoScript reverse transcriptase using random primers (A2801)) ...
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... Orsay virus RNA1 level was quantified by qPCR using 1 μl of 1/5 diluted cDNA (GoTaq Promega A6001) and run on QuantStudio 3 Real Time PCR system (RNA1 qPCR primers GW194 and GW195)23.
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... 1 μl of the supernatant was used to make cDNA (Promega GoScript reverse transcriptase using random primers (A2801)) ...
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bioRxiv - Bioengineering 2024Quote: The viability of human fibroblasts embedded in the bioprinted gel constructs was analyzed by monitoring the metabolic activity through the fluorimetric resazurin reduction method (CellTiter-Blue, Promega, G8080) at 1 ...
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... cDNA was diluted at 1/80 and qPCR were performed using 1 μl of diluted cDNA (GoTaq Promega) and run on QuantStudio 3 Real Time PCR system ...
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bioRxiv - Genomics 2024Quote: ... We amplified the Cytochrome c oxidase subunit I (COI) for individual abdomen DNA using the GoTaq (Promega) protocol ...
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bioRxiv - Microbiology 2024Quote: ... 100 μL of Bright-GloTM reagent (CAT#E2610, Promega) that was just warmed to room temperature was then added to each well ...
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bioRxiv - Microbiology 2024Quote: ... high capacity Magne streptavidin beads (Promega CAT#V7820) were first exchanged into PBS buffer ...
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bioRxiv - Biophysics 2024Quote: ... Samples were diluted to 1M urea using a stock of 50mM ammonium bicarbonate and trypsin (Promega) was added at a 1:50 enzyme-tosubstrate ratio and incubated overnight at 37 °C while shaking at 600 r.p.m ...
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bioRxiv - Biophysics 2024Quote: Transient transfection of HCN4 constructs and/or LRMP was performed using Fugene6 (Promega, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... CellTox (Promega, G8741), Annexin V Alexa568 (ThermoFisher ...
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bioRxiv - Molecular Biology 2024Quote: ... RNase inhibitor (Recombinant RNasin 20 U/ml; Promega) and 0.15 % Tween-20 ...
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bioRxiv - Molecular Biology 2024Quote: ... and RNase inhibitor (RNasin; Promega)] ...
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bioRxiv - Molecular Biology 2024Quote: ... The template DNA fragments were PCR-amplified using the psiCHECK2 plasmid (Promega) with 5′-TAATACGACTCACTATAGG-3′ and 5′-CACACAAAAAACCAACACACAG-3′ (Rluc) ...
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bioRxiv - Neuroscience 2024Quote: ... Prior to overnight digestion at 37 °C using modified porcine trypsin (Mass Spectrometry Grade, Promega, Madison, WI, USA) at an enzyme–protein ratio of 1:80 ...
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bioRxiv - Plant Biology 2024Quote: ... Reverse transcription was achieved from 1 𝜇g of total RNA with M-MLV reverse transcriptase (RNase H minus, Point Mutant, Promega, USA) using an anchored oligo(dT)20 primer ...
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bioRxiv - Developmental Biology 2024Quote: ... Reporter expression was quantified with the Nano-Glo Dual-Luciferase Reporter Assay System (Promega) in a Synergy H1 Hybrid Multi-Mode Microplate Reader (Thermo).
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bioRxiv - Developmental Biology 2024Quote: ... To quantify the activity of reports groups of 5 embryos were collected 8 hours post-injection in triplicates and lysed in 100 mL of lysis buffer (Promega). Reporter expression was quantified with the Nano-Glo Dual-Luciferase Reporter Assay System (Promega ...
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bioRxiv - Genomics 2024Quote: Total RNA was extracted from FFPE tumor patient samples using the ReliaPrep™ FFPE Total RNA Miniprep System (Promega). RNA Integrity was determined using the Fragment Analyzer ...
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bioRxiv - Microbiology 2024Quote: ... and digested by adding 0.2Lμg of trypsin-LysC (Promega) for 1Lh at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA was extracted from lymphoma derived cell lines as described above and PCR amplified using sgRNA specific primers (Supplementary Table 3, p53 primers as previously described (13) and GoTaq Green Master Mix (Promega). PCR products were amplified a second time using indexing primers ...
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bioRxiv - Cancer Biology 2024Quote: ... Integrated sgRNAs in each lymphoma sample were amplified from 100 ng of genomic DNA using GoTaq Green Master Mix (Promega) and indexing primers with unique overhangs (12) ...
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bioRxiv - Cancer Biology 2024Quote: ... Lactate-Glo Assay (#J5021, Promega) and Glucose-Glo Assay (#J6021 ...
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bioRxiv - Cancer Biology 2024Quote: ... GSH/GSSG-Glo Assay (#V6611, Promega), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and Glucose-Glo Assay (#J6021, Promega), respectively ...
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bioRxiv - Molecular Biology 2024Quote: ... 5–10 embryos were collected at 3 hpf and lysed in Passive Lysis Buffer (Promega) containing cOmplete Protease Inhibitor Cocktail (Sigma‒Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... PCR were carried out with Green Taq DNA polymerase (Promega) on cDNA using primers described in Table S4 ...
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bioRxiv - Microbiology 2024Quote: ... Treatments did not affect bacterial or cell viability as assessed by Lactate dehydrogenase release in the culture supernatant (LDH cytotoxicity kit®, Promega, according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was extracted from 2% agarose gel and purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) according to the manufacturer’s instructions and quantified using a Quantus Fluorometer (Promega, USA). Normalization was performed the counts of individual OTUs in a sample by dividing the total counts of all OTUs within that sample followed by a multiplication by resulting in relative abundance expressed.
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bioRxiv - Microbiology 2024Quote: Viral genomes were purified using Wizard genomic DNA purification kit (PROMEGA). To determine the amplification kinetic ...
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bioRxiv - Microbiology 2024Quote: ... before in-gel digestion using modified trypsin (Promega, sequencing grade) as previously described (42) ...
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bioRxiv - Microbiology 2024Quote: Furimazine was purchased from Promega (N1610) and used according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 μM amino acid mixture without leucine (Promega), and 1 U/ml ScriptGuard RNase inhibitor (CELLSCRIPT)] ...
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bioRxiv - Molecular Biology 2024Quote: Rluc mRNA and Fluc mRNA were incubated with rabbit reticulocyte lysate (RRL, Promega) to generate the mRNA-ribosome complex ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10 μl of premix [100 μM amino acid mixture without methionine (Promega), 100 μM amino acid mixture without leucine (Promega) ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 ng/μl of mRNA and 6 ng/μl QuantiLum Recombinant Luciferase protein (Promega) were coinjected ...
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bioRxiv - Molecular Biology 2024Quote: Cells were seeded in white 96-well plates and incubated with the RealTime-Glo MT Cell Viability Assay (Promega). Luminescence was detected using a GloMax-96 instrument (Promega).
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bioRxiv - Molecular Biology 2024Quote: ... The template DNA fragments were PCR-amplified using the psiCHECK2 plasmid (Promega) with 5′-TAATACGACTCACTATAGG-3′ and 5′-CACACAAAAAACCAACACACAG-3′ (Rluc) ...
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bioRxiv - Immunology 2024Quote: BAC-derived MCMV was reconstituted by transfection of BAC DNA into NIH-3T3 cells (ATCC CRL-1658) using FuGENE HD transfection reagent (Promega, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The converted DNA was desalted using the Wizard DNA clean-up system (Promega), desulphonated ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were prepared using the SP3 protocol(78, 79) and trypsin (sequencing grade, Promega) was added in an enzyme to protein ratio 1:50 for overnight digestion at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... ROS-Glo™ H2O2 Assay (G8820, Promega) was used ...
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bioRxiv - Molecular Biology 2024Quote: ... Luminescence was detected using a GloMax-96 instrument (Promega).
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bioRxiv - Immunology 2024Quote: ... the samples were quenched with 10 mM DTT and digested overnight at 37°C with Trypsin gold (Promega, WI, USA). The digestion was stopped and the surfactant was cleaved by treating samples with 200 mM HCl (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were digested on-beads with Trypsin (Promega) with ratio enzyme:proteins 1:20 in 50 mM ammonium bicarbonate and performed overnight at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The luciferase activities were measured using the Dual-Glo Luciferase Assay System and a GloMax 20/20 luminometer (Promega). The intensity of Fluc activity was normalized to the intensity of Rluc activity ...
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bioRxiv - Plant Biology 2024Quote: ... and DNAse treated using RQ1 (Promega, USA). Reverse transcription was achieved from 1 𝜇g of total RNA with M-MLV reverse transcriptase (RNase H minus ...