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Citations for Promega :
1201 - 1250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... 6 µL of Fugene 6 (Promega), and OptiMem (ThermoFisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Cells were then collected and washed before cell lysis and addition of luciferin substrate from the Bright-Glo Luciferase Assay System (Promega). The resulting luminescent signal was measured using a Tecan Infinite M200 Pro ...
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bioRxiv - Genetics 2024Quote: ... in 2x SSCT (0.1% Tween-20 in 2x SSC (Promega, Cat# V4261)) for 5 min at RT ...
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bioRxiv - Genetics 2024Quote: ... PCR was performed using Go-Taq Green Master Mix (Promega, Madison, WI) and resulting products were resolved by 3% agarose gel electrophoresis ...
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bioRxiv - Immunology 2024Quote: ... samples were taken and incubated with RealTime-Glo™ MT (Promega) per manufacturer’s instructions in a CO2 and temperature-controlled plate reader (Tecan Spark Cyto) ...
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bioRxiv - Immunology 2024Quote: ... or Lactate-Glo (Promega) following manufacturers’ protocols ...
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bioRxiv - Immunology 2024Quote: ... Purified EDIII antigens were biotinylated using HaloTag PEG biotin ligand (Promega), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... or pMXs-IRES-dsRed-mIRF4 into Plat-E34 with Fugene6 Transfection Reagent (#E2691, Promega) as described in our previous studies35,36 ...
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bioRxiv - Immunology 2024Quote: Total RNA was extracted from cells using the ReliaPrep RNA Cell Miniprep System (#Z6012, Promega) and from skin using RNAzol RT Reagent (#RN190 ...
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bioRxiv - Genetics 2024Quote: ... Virus was concentrated using PEG virus precipitation (Promega # V3011). In brief ...
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bioRxiv - Immunology 2024Quote: ... CellTiter-Glo 2.0 Cell Viability Assay (Promega Cat. No. G9242) was used according to the manufacturer’s instructions as a measure of proliferation ...
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bioRxiv - Immunology 2024Quote: ... 100mM D-luciferin was added to Atmospheric Media at 1:1000 for 100μM D-luciferin (Promega, CAT#E1602). Cells cultured in Atmospheric Media were kept at 37⁰C in atmospheric conditions ...
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bioRxiv - Immunology 2024Quote: ... Each mutation locus was amplified in separate PCR reactions using GoTaq Green Master Mix (Promega Cat. No. M7123). PCR reactions were held at 95 °C for two minutes followed by 35 cycles of 45 seconds at 95°C for denaturation ...
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bioRxiv - Genetics 2024Quote: ... 40μL of Cell Titer Glo reagent (Promega) was added to each well ...
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bioRxiv - Genetics 2024Quote: ... The qRT-PCR reactions were conducted in a 10 μL volume using the GoTaq qPCR Master Mix chemistry (Promega, Madison, Wisconsin, USA) and a CFX384 Touch Real-Time PCR Detection System (BioRad ...
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bioRxiv - Genetics 2024Quote: ... We utilized the GoScript Reverse Transcriptase Kit (Promega, Madison, Wisconsin, USA) in accordance with the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... Cell viability was measured using CellTiter-Glo Luminiscent Cell Viability Assay (Promega, G7571) and Glomax Discover plate reader (Promega) ...
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bioRxiv - Genetics 2024Quote: ... and Glomax Discover plate reader (Promega). The measurement was performed every 24 hours starting from the day after seeding (24h ...
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bioRxiv - Immunology 2024Quote: RNA from cells was isolated using the ReliaPrep RNA Miniprep System (Promega). RNA was reverse transcribed using UltraScriptTM cDNA Synthesis Kit (PCR Biosystems ...
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bioRxiv - Immunology 2024Quote: ... 5-GGATCCACACGGTGCAAAGAGAGACCC-3’ and 5′-TCGGCCTTTCAGACTAATCTTATCAGC-3’ The PCR products were gel purified and cloned into the pGEM-T vector (Promega; Madison, WI, USA). The inserted PCR fragments of individual clones were sequenced by Tsing KE Biological Technology ...
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bioRxiv - Immunology 2024Quote: ... genomic DNA was extracted using a Tecan automation workstation (Promega). DNA whole-exome sequencing (WES ...
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bioRxiv - Genomics 2024Quote: DNA was extracted from blood samples with Wizard Genomic DNA Purification kit (Promega) and quality was verified in 1% agarose gel electrophoresis ...
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bioRxiv - Genomics 2024Quote: ... The reactions were purified with the ProNex Size-Selective Purification System kit (Promega) and the concentration was rechecked by fluorometry in the Qubit 3.0 instrument (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2024Quote: ... and 0.01% Digitonin (Promega, G9441). The reaction was quenched with ATAC RSB with 0.1% Tween-20 and centrifugation ...
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bioRxiv - Genomics 2024Quote: DNA was extracted from cell pellets using the Wizard Genomic DNA Extraction Kit (Promega UK, Catalogue number: A1120). For S ...
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bioRxiv - Genomics 2024Quote: ... Celltiter-Glo solution (Promega, ref. G7573) was added to cells grown in 96-well plates ...
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bioRxiv - Genomics 2024Quote: ... Cell titer-Glo solution (Promega, ref. G7573) was added to cells grown in 96-well plates and measurements were made in a VICTOR3 Multilabel Plate Reader (PerkinElmer).
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bioRxiv - Genomics 2024Quote: ... with 300 µl of trypsin/LysC in 50 mM TEAB pH 8.0 (Trypsin Gold, Promega and LysC ...
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bioRxiv - Genomics 2024Quote: ... cDNA samples were then combined with GoTaq qPCR Master Mix SYBR Green (Promega, ref. A6002) in 384 well-plates ...
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bioRxiv - Genomics 2024Quote: RNA was extracted using the ReliaPrep RNA kit (Promega, ref. Z6012) following manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Trypsin digestion was done by adding 5 µL reductively methylated trypsin (Promega, Madison, USA) to each sample and incubating the samples overnight at 37°C ...
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bioRxiv - Genomics 2024Quote: ... The final DNA yield (17.3 µg) was quantified using a Quantus Fluorometer (QuantiFluor ONE dsDNA Dye assay; Promega, Madison, WI). The size distribution of the HMW DNA was estimated using the Femto Pulse system (Agilent ...
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bioRxiv - Immunology 2024Quote: Extracellular DNA release was quantified from the supernatant of NETosis-stimulated neutrophils using QuantiFluor dsDNA system (Promega; Madison, WI) per manufacturer instructions ...
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bioRxiv - Genomics 2024Quote: ... and 0.01% digitonin (Promega, G9441)) for 3 min on ice ...
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bioRxiv - Genetics 2024Quote: ... LDH release was quantified using CytoTox 96 Non-Radioactive Cytotoxicity Assay (Promega #G1780) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... using the GoTaq Master Mix (Promega) according to the manufacturer’s protocols ...
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bioRxiv - Genomics 2024Quote: ... We prepared DNA from ear tissue samples of 1036 HBS-affected pigs using the Promega Maxwell RSC DNA System (Promega, Dübendorf, Switzerland) and sent it to Gencove for low-pass sequencing (https://gencove.com/) ...
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bioRxiv - Genetics 2024Quote: ... part of the ReliaPrep RNA Tissue Miniprep System (Promega, Madison, Wisconsin, USA), which was used to extract total RNA from approximately 30 brains for each sample according to manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... cDNA was synthesized using the ImProm-II™ Reverse Transcription System (Promega, USA). The mRNA composition was established by PCR with plasmid-specific primers ...
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bioRxiv - Genomics 2024Quote: ... Input DNA was spiked with unmethylated lambda DNA (0.5%) (Promega, #D1521). Replicate bisulfite libraries were generated with the CEGX TrueMethyl® Whole Genome Kit (CEGX ...
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bioRxiv - Genomics 2024Quote: ... QuantiFluor® dsDNA System (Promega Corp., Madison, WI, USA), and Kapa Illumina Library Quantification qPCR assays (Kapa Biosystems) ...
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bioRxiv - Genomics 2024Quote: ... Standard PCR was performed on cDNAs using the GoTaq DNA polymerase (Promega). All primer sequences can be found in Table S23.
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bioRxiv - Developmental Biology 2024Quote: ... Samples were then treated with 10 µg/ml Proteinase K (Promega V3021) in PTw for 30min ...
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bioRxiv - Developmental Biology 2024Quote: ... Digoxigenin-labelled or Fluorescein-labelled mRNA probes were either prepared as PCR probes with primers listed in Table S4 using the Kit GoTaq green master mix (Promega) or as described (Orgeur et al. ...
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bioRxiv - Developmental Biology 2024Quote: ... and also with PCR Master Mix (M7502, Promega), Taq polymerase (EP0402 ...
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bioRxiv - Developmental Biology 2024Quote: ... and the ONE-Glo™ Luciferase Assay System (E6110, Promega) exactly as described in 16.
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bioRxiv - Developmental Biology 2024Quote: ... and phRL-TK (E2241, Promega) as controls and analyzed for promoter activation in the presence of 3mg/l recombinant human SPARC (SRP3159 ...
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bioRxiv - Developmental Biology 2024Quote: ... using the Dual-Luciferase® Reporter Assay System (E1910, Promega) and the ONE-Glo™ Luciferase Assay System (E6110 ...
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bioRxiv - Developmental Biology 2024Quote: CSCs and cells from 9-day-old CBs were transiently transfected with the nkx2.5 promoter-reporter plasmid pNKE24 44 and with pGL3-Basic (E1751, Promega) and phRL-TK (E2241 ...
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bioRxiv - Cell Biology 2024Quote: 35S-methionine-labelled prey proteins (Grip91, Sas4) were produced in vitro using the TnT T7 Quick Coupled IVTT kit (L1170, Promega). The detailed protocol of the GST-IVTT binding assay is described previously 52.