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Citations for Promega :
9301 - 9350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... PCR amplification used GoTaq Hot Start Polymerase (Promega). PCR conditions were 95°C for 3 min ...
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bioRxiv - Physiology 2023Quote: ... and 10 ng of Renilla luciferase reporter plasmid pRL-RSV (Promega), in the presence or absence of indicated expression vectors using FuGene 6 transfection reagent (Promega) ...
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bioRxiv - Physiology 2023Quote: ... using FuGENE HD Transfection Reagent (Promega #E2311) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Individuals of the F2 progeny were analysed by PCR genotyping using GoTaq2 DNA polymerase (Promega) according to standard protocols ...
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bioRxiv - Plant Biology 2023Quote: ... RNase-Free DNase (Promega, USA) treatment of the isolated RNA samples was done to remove any DNA contamination ...
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bioRxiv - Plant Biology 2023Quote: ... The 16S rDNA PCR-amplified fragments were purified by Wizard® SV Gel and PCR Clean-Up System (Promega, New Delhi, India) and digested by HaeIII (SRL ...
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bioRxiv - Cell Biology 2023Quote: ... the samples were directly spiked with 50 pg of unmethylated lambda phage DNA (Promega), followed by bisulfite treatment and library construction as described for the FGO libraries ...
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bioRxiv - Cancer Biology 2023Quote: ... regions of mtDNA were cloned from donor cell lines into p-GEM-T Easy vector (Promega, A1360). Samples were then diluted to specific concentrations and analyzed to evaluate probe specificity.
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bioRxiv - Cell Biology 2023Quote: ... Samples were directly spiked with 5 pg of unmethylated lambda phage DNA (Promega) and subjected to bisulfite treatment and library construction using the post-bisulfite adaptor tagging (PBAT ...
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bioRxiv - Molecular Biology 2023Quote: ... The Nano-Glo® Live Cell Assay System was purchased from Promega and used as received (Cat #N2012) ...
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bioRxiv - Cell Biology 2023Quote: ... The bacmid was transfected into Sf9 cells using Fugene HD transfection reagent (Promega). The obtained viral stock was amplified and used for protein expression ...
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bioRxiv - Neuroscience 2023Quote: ... the plasmid pGL4.83h[RlucP/Puro] (Promega) that contains the human EF1a promoter in upstream to the Renilla luciferase (Rluc ...
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bioRxiv - Neuroscience 2023Quote: ... 1,200 ng of ORs tagged with the first 20 amino acids of human rhodopsin (rho-tag) at the N-terminal ends in pCI mammalian expression vector (Promega) and 30 ng eGFP were transfected using Lipofectamine 2000 (11668019 ...
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Multi-omics analysis reveals signatures of selection and loci associated with complex traits in pigsbioRxiv - Genomics 2023Quote: ... 400-bp DNA fragments centring on the SNPs were first amplified and then inserted into the PGL4.23 Dual-Luciferase Expression Vector (Promega, Madison, WI, USA). Primers were designed using Primer Premier5 and listed in Supplementary Table 21.
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Multi-omics analysis reveals signatures of selection and loci associated with complex traits in pigsbioRxiv - Genomics 2023Quote: ... A Dual-Luciferase Reporter Assay System (Promega) was used to quantify luciferase activities following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and 75 μg of rho-tagged OR in the pCI mammalian expression vector (Promega) were transfected 18–24 h before odorant stimulation using Lipofectamine 2000 (11668019 ...
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bioRxiv - Microbiology 2023Quote: ... The HiBiT value measured by Nano Glo HiBiT lytic detection system (Promega, Cat# N3040) indicates the amount of p24 HIV-1 antigen ...
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bioRxiv - Microbiology 2023Quote: ... Firefly and Renilla luciferase levels in cells were measured sequentially according to the Dual-Glo Luciferase assay protocol (Promega).
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bioRxiv - Microbiology 2023Quote: ... The amplicon cloned into pGEM T-EASY (Promega). An 804 bp portion of the hsdM1 coding sequence was deleted by inverse PCR with the inclusion of a unique BamHI site in each of the following primers ...
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bioRxiv - Neuroscience 2023Quote: ... 10 μg pGloSensor-20F plasmid (Promega), 5 ug of RTP1S plasmid (only for OR1A1 and its mutants) ...
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bioRxiv - Neuroscience 2023Quote: The GloSensor cAMP assay (Promega) was used to determine real-time cAMP levels downstream of OR activation in HEK293T cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was assessed using a commercial CellTiter-Glo (CTG) luminescence-based assay (Promega) as described in 2D cell viability assay.
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was assessed using a commercial CellTiter-Glo (CTG) luminescence-based assay (Promega). The 96-well plates were equilibrated to room temperature before the addition of diluted CTG reagent (100 μL ...
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bioRxiv - Systems Biology 2023Quote: ... and digested according to the manafacturer’s protocol for 1 hour at 50 °C by Trypsin Platinum (Promega, WV), digested material was dried in speedvac (Eppendorf ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested using LysC 1:100 enzyme:proteins ratio for 4 hours (Wako sequencing grade, 125-05061) and trypsin 1:100 enzyme:proteins ratio for 16 hours (Promega sequencing grade, V5111). The digested proteins were then acidified with 10% (v/v ...
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bioRxiv - Neuroscience 2023Quote: Intracellular ATP in assembloids was measured using luminescence based CellTiter-Glo® 3D Cell Viability Assay (Promega, G9681). Three organoids per cell line and per condition were transferred each in one well of the imaging plate (PerkinElmer ...
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bioRxiv - Systems Biology 2023Quote: ... 37.5 µL of 8 M Urea and digested overnight at room temperature with 1 µg/µL endoproteinase Lys-C (Wako) followed by digestion with sequencing-grade trypsin (Promega) at a final concentration of 1 ng/μL 6 h at 37 °C ...
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bioRxiv - Genetics 2023Quote: ... and luciferase activity measured after 24 hours using the Dual-Luciferase Reporter Assay System (Promega, #E1980) and the Synergy H1 Hybrid Multi-Mode Microplate Reader with dual injectors (BioTek ...
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bioRxiv - Cell Biology 2023Quote: ... overnight with Tryp-LysC (Promega), and then 4 hours in mass-spectrometry-grade trypsin (Promega) ...
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bioRxiv - Cell Biology 2023Quote: Mouse livers were homogenized in Luciferase Culture Lysis Reagent (Promega). Lysates were clarified by centrifugation at 13,000 × g for 20 min at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: VPS34 kinase activity was assessed via the ATP Glo (Promega) luminescence assay ...
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bioRxiv - Bioengineering 2023Quote: ... and 100 μl of Luciferase Assay Reagent (Promega) was treated right before detection ...
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bioRxiv - Molecular Biology 2023Quote: ... Histones were then digested with 1 µg of sequencing grade trypsin (Promega) diluted in 50mM ammonium bicarbonate (1:20 ...
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bioRxiv - Cell Biology 2023Quote: HeLa cells were transfected using 2 µg of plasmid DNA and 6 µL of FuGENE (Promega) for gene editing or using a NEPA21 electroporation system (Nepa Gene ...
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bioRxiv - Cell Biology 2023Quote: ... Amounts of protein binding to M.tb were quantified by colorimetric measurements by absorbance at 450 nm using a GloMax plate reader (Version 3.0; Promega Corporation), and plotted based on absorbance values relative to the baseline (negative control ...
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bioRxiv - Cell Biology 2023Quote: ... and applied to S-Traps (micro; Protifi) for tryptic digestion (sequencing grade; Promega, Madison, WI) in 50 mM TEAB ...
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bioRxiv - Cell Biology 2023Quote: ... gel pieces were dried and rehydrated with 12.5 ng/mL trypsin (Promega, Madison, WI) solution in 25 mM ammonium bicarbonate on ice for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... and bacterial bioluminescence was measured in relative luminescence units (RLUs) every 24 h for up to seven days using a GloMax Multi Detection System (Promega) (35) ...
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bioRxiv - Cell Biology 2023Quote: ... and P4 hESCs using the Wizard® HMW DNA Extraction Kit (#A2920, Promega, USA) for whole-genome sequencing on the Illumina NovaSeq platform ...
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bioRxiv - Cell Biology 2023Quote: ... Luciferase activity was assayed using Dual-Luciferase Reporter Assay Kit (Promega) in 96-well black plates ...
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bioRxiv - Developmental Biology 2023Quote: ... Sample cleanup was performed using the SP3 method 83 and on-bead digestion was performed overnight using Trypsin/Lys-C (Promega). Eluted tryptic peptides from SP3 beads were pre-fractionated by high pH reverse phase chromatography into 96 individual fractions using a 64-minute gradient from 1% to 50% solvent B (A ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Purification was performed to remove adaptors and primer dimers using the Wizard Genomic DNA Kit (Promega) and quality was verified by measuring the quantity and length of the fragments ...
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bioRxiv - Cell Biology 2023Quote: ... Luciferase activity was measured using a Dual–Luciferase Reporter Assay System (Promega, E1500).
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bioRxiv - Immunology 2023Quote: ... then combined in a 1:1 ratio with Nano-Glo® Luciferase solution (Promega, Ref N1150). A Promega GloMax plate reader was used to measure luminescence in technical triplicate for each sample ...
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bioRxiv - Immunology 2023Quote: ... Three biological replicates for each condition were digested with Lys-C (Alpha Laboratories) and trypsin (Promega) “on beads” as previously described [44] ...
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bioRxiv - Developmental Biology 2023Quote: RNA was isolated using the SV Total RNA Isolation System kit (Promega, Z3105). RT-PCR for mRNA transcripts was performed by using the ImProm-II Reverse Transcription System kit (Promega A3800) ...
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bioRxiv - Developmental Biology 2023Quote: ... Single transformed ampicillin resistant colonies were grown in LB + Ampicillin and their DNA was extracted by the Wizard® Plus SV Minipreps DNA Purification System kit (Promega, A1460). Clones with correct insertions were evaluated by Sanger sequencing (LightRun tube ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-PCR for mRNA transcripts was performed by using the ImProm-II Reverse Transcription System kit (Promega A3800). Real Time PCR was performed with Applied Biosystems Fast SYBR Green Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... and the DNA fragments encoding the designs were amplified following a colonyPCR protocol using GoTaq® Green DNA polymerase master mix (#M7122; Promega) and T7 reverse and forward primers ...
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bioRxiv - Biochemistry 2023Quote: ... and developed using a reinforced chemiluminescent substrate system (Promega).