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Citations for Promega :
9351 - 9400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... followed by cDNA synthesis using GoScript Reverse transcription mix (Promega). The detection of RIG-I ...
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bioRxiv - Cell Biology 2023Quote: ... following instructions from the SDM kit (Promega). Promoter elements used in luciferase reporter expression analyses were cloned into the pGL3-basic vector (Promega).
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bioRxiv - Cell Biology 2023Quote: ... cells were transiently transfected with 1.5 μg of plasmid using FuGENE 6 transfection reagent (#E2692, Promega), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... 200 U/mL RNasin (#N2111, Promega), 100 µg/mL cycloheximide (#7698 ...
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bioRxiv - Developmental Biology 2023Quote: ... modified hypotonic lysis buffer with a 10-fold higher concentration of Cycloheximide and Recombinant RNasin Ribonuclease Inhibitor (Promega) was used instead of RNase OUT ...
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bioRxiv - Genetics 2023Quote: ... Secondary horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG antibodies (Promega) were used at 1 ...
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bioRxiv - Genomics 2023Quote: ... PCR was performed in a 20 µL reaction containing 1X Colorless GoTaq Flexi Buffer (Promega), 1.5 mM MgCl2 ...
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bioRxiv - Cell Biology 2023Quote: Immortalized DMDΔ52 myoblasts were co-transfected with 1.5ug of each plasmid containing the selected sgRNAs using ViafectTM (Promega) transfection reagent (1:5 ratio) ...
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bioRxiv - Cell Biology 2023Quote: ... Promoter elements used in luciferase reporter expression analyses were cloned into the pGL3-basic vector (Promega).
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bioRxiv - Cell Biology 2023Quote: ... Cell proliferation was measured using the CellTiter 96 aqueous non-radioactive cell proliferation assay (MTS) (Promega) as previously described (Poyatos-García et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Relative gene expression was determined using the Dual-Luciferase Reporter assay system (Promega) on a luminometer.
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bioRxiv - Cell Biology 2023Quote: ... Samples were then digested with 250 ng trypsin (Promega, cat#V5113) on a thermomixer with 1200 r.p.m ...
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bioRxiv - Cell Biology 2023Quote: ... pulsed for 20 min with 100 nM TMR ligand (Promega, G8251), washed twice in 1x DPBS ...
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bioRxiv - Cell Biology 2023Quote: ... in-gel digestion with trypsin (Promega) overnight ...
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bioRxiv - Cell Biology 2023Quote: ... and ImProm II reverse transcriptase (Promega). Quantitative RT-PCR was performed using a Thermal Cycler Dice Real Time System II (Takara Bio Inc. ...
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bioRxiv - Cell Biology 2023Quote: ... The NO concentration in the culture medium was measured using the Griess reagent system (Promega, Tokyo, Japan). To determine the direct effects of mannosidase on the matrix ...
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bioRxiv - Cell Biology 2023Quote: Production of hydrogen peroxide (H2O2) in WT and Alas1 KO brown adipocytes was measured using the ROS-GloTM H2O2 assay kit (Promega #G8820) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The PCR products were cloned in the pGEM T Easy vector system (Promega) according to the manufacturer’s instructions and sequenced (Eurofins Genomics and SourceBioscience) ...
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bioRxiv - Immunology 2023Quote: ... and pMD2.G were co-transfected by Fugene HD Transfection Reagent (Promega Corporation, Madison, WI). The supernatant was collected at 48 h ...
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bioRxiv - Immunology 2023Quote: ... The linearized plasmid was then purified using the Wizard Plus SV Miniprep DNA Purification System (Promega), and saRNA was transcribed in vitro using HiScribe T7 High Yield RNA Synthesis Kit (NEB) ...
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bioRxiv - Immunology 2023Quote: ... FuGENE HD (Promega, E2311), HALT protease inhibitor cocktail (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... The inserts and plasmid backbones were purified with Promega Wizard SV gel and PCR Cleanup System (Promega). Purified inserts and backbones were mixed in a molar 3:1 ratio ...
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bioRxiv - Genetics 2023Quote: ... 10 µL of CellTiter-Blue Viability reagent (Promega) was added to each well ...
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bioRxiv - Immunology 2023Quote: ... 100 μl of culture medium were discarded and addition with 100 μl of Bright-Glo luciferase reagent (Promega, E6120) in each well ...
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bioRxiv - Genomics 2023Quote: ... Reference allele enhancer sequences for hZRS and hs737 were obtained via PCR cloning from human genomic DNA (Promega, G304A). Primers used for each enhancer sequence are outlined in Table S2 ...
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bioRxiv - Genomics 2023Quote: ... 10 U RNAsin (Promega), 1 × Superscript II First-Strand Buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... cloned into pGEM-T (Promega) and used to synthesize digoxigenin-labeled probes by in vitro transcription using SP6 or T7 polymerases (Thermo-Fisher) ...
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bioRxiv - Genetics 2023Quote: ... The first-strand cDNA from a colonic sensory neuron was used as template in a PCR reaction containing 1×GoTaq reaction buffer (Promega Madison, WI), 20 mM outer primers ...
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bioRxiv - Cell Biology 2023Quote: ... Total RNA was reverse transcribed using oligo(dT) primers and MMLV reverse transcriptase (Promega, PR-M1705) to generate cDNA ...
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bioRxiv - Biochemistry 2023Quote: ... alkylated by iodoacetamide and digested overnight with trypsin (sequence modified, Promega). An aliquot of the digest was analysed by LC- MS/MS on Q ExactiveTM Plus Orbitrap™ mass spectrometer equipped with an UltiMate 3000 RSLCNano LC system (both from ThermoFisher) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We quantified all 4,754 samples using the GloMax Explorer System (Promega Corporation, Madison, WI, USA) and prepared libraries for target enrichment using the SeqCap EZ – HyperPlus kit (Roche Sequencing Solutions ...
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bioRxiv - Genetics 2023Quote: ... and amplified by PCR using the GoTaq Master Mix (Promega, Charbonnières les Bains, France). We employed specific primers for PTGER1 ...
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bioRxiv - Developmental Biology 2023Quote: ... The prospero 3’UTR [2131bp] was subcloned into the pmirGlo Dual-Luciferase expression vector (Promega) downstream of the firefly luciferase gene ...
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bioRxiv - Cancer Biology 2023Quote: ... bioluminescence released by RMS target cells was measured by using ONE-GloTM Luciferase assay system (Promega, #E6120) with GLO-Max device (Promega) ...
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bioRxiv - Cancer Biology 2023Quote: ... with GLO-Max device (Promega). The results were analyzed with Microsoft Excel and GraphPad Prism 9.
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bioRxiv - Developmental Biology 2023Quote: ... Bacteria were plated onto pre-warmed kanamycin resistance LB agar plates in the presence of X-gal (Promega, #V3941) for blue/white screening ...
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bioRxiv - Immunology 2023Quote: ... horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG antibody (1:10000) (Promega, Madison, WI, USA), diluted in 3% (w/v ...
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bioRxiv - Immunology 2023Quote: ... RH5ΔNC-specific B cells were identified as live CD19+ IgG+ RH5ΔNC-APC+ RH5ΔNC-PE+ cells and single cell sorted into 96-well plates containing 10µL/well lysis buffer (10mM Tris [T3038, Merck], 1 unit/mL RNasin Ribonuclease Inhibitor [N2515, Promega]) and frozen at -80 °C.
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bioRxiv - Genomics 2023Quote: ... Total RNA from ED and TE samples was isolated using the ReliaPrepTM RNA Cell Miniprep System (Promega, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... NanoGlo (Promega, #N1150) reagent was added at 25µLs per well ...
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bioRxiv - Microbiology 2023Quote: ... and then collected and used to quantify lactate dehydrogenase (LDH) release (in a 50 μl aliquot) using the CytoTox 96 Non-Radioactive Cytotoxicity Assay kit (Cat No. G1780, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... the presence of other main circulating arboviruses was evaluated by RT-qPCR using GoTaq® 1-Step RT-qPCR System (Promega, Madison, WI, USA). Specific diagnosis primers were used for Zika [30] ...
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bioRxiv - Microbiology 2023Quote: Total nucleic acid extraction from swab and tissue samples was performed using ReliaPrep™ Viral TNA Miniprep System (Promega, Madison, WI, USA). Swab samples were simply inverted in the tube and briefly centrifuged prior to extraction ...
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bioRxiv - Immunology 2023Quote: ... and the GloMax Navigator Microplate Luminometer (Promega). The 50% neutralizing dilution (ND50) ...
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bioRxiv - Immunology 2023Quote: ... cells were further cultivated for 36 h and harvested for luciferase assay using the Nano-Glo luciferase assay system (Promega; cat. no. N1120) and the GloMax Navigator Microplate Luminometer (Promega) ...
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bioRxiv - Immunology 2023Quote: ... the Renilla luciferase (Rluc) gene was PCR-amplified from phRL-TK (Promega, Madison, WI, USA, cat. no. E6241), and the eGFP gene was PCR-amplified from a codon-optimized synthetic gene fragment obtained from Bionics Inc ...
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bioRxiv - Immunology 2023Quote: ... and the absorbance at 450 nm was read using the GloMax-Multi Detection System (Promega).
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bioRxiv - Immunology 2023Quote: ... the cells were harvested and subjected to a luciferase assay using the Rluc Assay System (Promega; cat. no. E2820). Luminance (ALU ...
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bioRxiv - Immunology 2023Quote: ... Luminance (ALU) measured in a GloMax Navigator Microplate Luminometer (Promega) was normalized to microgram lysate protein.
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bioRxiv - Microbiology 2023Quote: ... the infected cells were lysed with a Bright-Glo Luciferase Assay System (Promega, cat# E2620) and the luminescent signal was measured using a Centro XS3 LB960 plate reader (Berthhold Technologies ...