Labshake search
Citations for Promega :
9401 - 9450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... cDNA was obtained using random hexamers (Promega) and Revert Aid reverse transcriptase (ThermoFisher Scientific ...
-
bioRxiv - Microbiology 2023Quote: The amplified products underwent purification using Quick PCR purification columns (Promega, Madison, WI, USA) and were subsequently sequenced with the Big Dye Terminator Cycle Sequencing Ready Reaction Kit on an Applied Biosystems analyzer (Applied Biosystems ...
-
bioRxiv - Microbiology 2023Quote: The region upstream of the genes of interest was amplified by PCR with proper primers (Table S4) and cloned into the pGEM-T easy plasmid (Promega). The insert was removed by digestion and subcloned into the pRKlacZ290 vector to generate transcriptional fusions to the lacZ gene ...
-
bioRxiv - Microbiology 2023Quote: ... we used the Kinase-Glo kit (Promega, V6728) to assess the activity of purified FruK.
-
bioRxiv - Microbiology 2023Quote: ... The eDNA was quantified using the QuantiFluor dsDNA dye (QuantiFluor dsDNA System, Promega, Madison, WI, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2023Quote: ... Luciferase Bright-Glo substrate (Promega) was added and emitted light was measured using a luminescence plate reader or IVIS Imaging System (Xenogen ...
-
bioRxiv - Microbiology 2023Quote: ... genomic DNA was extracted with the Maxwell 16-cell purification kit (Promega, https://www.promega.com). The DNA libraries were then prepared at the Institut Pasteur Mutualized Platform for Microbiology (P2M ...
-
bioRxiv - Immunology 2023Quote: ... The total RNA extracted was reverse transcribed using M-MLV reverse transcriptase (Promega, Madi-son, WI, USA) and using random primers for initiation ...
-
bioRxiv - Immunology 2023Quote: ... Pseudotyped virus were prepared by transfection of HEK293T cells using the Fugene HD transfection system (Promega), as previously described(42) ...
-
bioRxiv - Immunology 2023Quote: ... prepared by adding NanoDLR™ Stop & Glo® Substrate 1:100 into NanoDLR™ Stop & Glo® Buffer (Promega, USA), were added to the samples ...
-
bioRxiv - Immunology 2023Quote: ... a total of 50.000 chicken DF-1 cells were seeded in 24 well plates and were co-transfected 24h later with a vector plasmid containing the deletion mutant and a second plasmid for the expression of Firefly under the PGK promoter (Promega, USA). 24h after transfection ...
-
bioRxiv - Microbiology 2023Quote: ... and GloMax plate reader (Promega). Data were normalized to mock-treated ...
-
bioRxiv - Microbiology 2023Quote: CellTiter 96 AQueous One Solution (G3580, Promega) was used ...
-
bioRxiv - Microbiology 2023Quote: ... and GloMax plate reader (Promega). Data were normalized by subtracting Gluc signal from mock-infected cells ...
-
bioRxiv - Microbiology 2023Quote: ... cytotoxicity was assessed using the CellTiter-Glo luminescent cell viability kit (Promega). 3,000 cells/well of Vero E6 were seeded in white with clear bottom 384-well plates ...
-
bioRxiv - Microbiology 2023Quote: ... Residual DNA was digested with RNAse-free DNAse for 30 min (Promega), before enzyme and buffer were removed via column clean up (QIAGEN) ...
-
bioRxiv - Immunology 2023Quote: ... was isolated with Reliaprep RNA Tissue Miniprep System according to manufacturer instructions (Promega), followed by cDNA synthesis using GoScript Reverse transcription mix (Promega) ...
-
bioRxiv - Immunology 2023Quote: ... prepared by combining ONE-Glo™ EX Luciferase Assay Buffer with ONE-Glo™ EX Luciferase Assay Substrate in 1:1 ratio (Promega, USA). After measuring the signal of the Firefly luciferase in the GloMax® 20/20 Luminometer (Promega ...
-
bioRxiv - Immunology 2023Quote: ... After measuring the signal of the Firefly luciferase in the GloMax® 20/20 Luminometer (Promega, USA), 80 µl NanoDLR™ Stop & Glo® reagent ...
-
bioRxiv - Immunology 2023Quote: Total RNA was isolated from the spleen of the mallard duck (Anas platyrhynchos) using ReliaPrepTM RNA Tissue Miniprep System (Promega, USA), followed by cDNA synthesis using GoScript™ Reverse Transcription System (Promega ...
-
bioRxiv - Immunology 2023Quote: ... followed by cDNA synthesis using GoScript™ Reverse Transcription System (Promega, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... with MG-132 Inhibitor (Promega) was added to each well ...
-
bioRxiv - Immunology 2023Quote: ... and 120 min and measured again in the GloMax® 20/20 Luminometer (Promega, USA) to detect the NanoLucTM Luciferase ...
-
bioRxiv - Biochemistry 2023Quote: ... 40 μL of One-Glo-EX substrate (Promega) was added to each well and incubated on a plate shaker in the dark for 5 min before reading the relative luciferase units using a BioTek Neo2 plate reader ...
-
bioRxiv - Immunology 2023Quote: ... All secondary HRP-conjugated antibodies were obtained from Promega (Madison, WI). For immunofluorescence only ...
-
bioRxiv - Microbiology 2023Quote: ... Amplicons were cloned and inserted into a pGEM-T vector (Promega, WI) and transformed into competent DH5α E ...
-
bioRxiv - Immunology 2023Quote: ... in a 1.5:1 ratio with FuGene (#E2311, Promega). One day post transfection ...
-
bioRxiv - Immunology 2023Quote: ... HRP-anti-Mouse IgG (H+L) (Promega).
-
bioRxiv - Cancer Biology 2023Quote: ... in a GloMax 20/20 Luminometer (Promega) according to manufacturer instructions.
-
bioRxiv - Cell Biology 2023Quote: ... 2 μL of ONE-glo luciferase detection reagent (Promega) was dispensed per well and luminance signal recorded with an Envision plate reader (Perkin Elmer).
-
bioRxiv - Cancer Biology 2023Quote: ... Luminescence was then assayed using a GloMax Veritas Luminometer (Promega). To calculate the proportion of viable cells ...
-
bioRxiv - Molecular Biology 2023Quote: ... followed by incubation with S1 nuclease (20 U/ml) (Promega M5761) in S1 buffer for 60 min at 37 °C
-
bioRxiv - Genomics 2023Quote: ... The bacteriomes were dissected and DNA was extracted using the High Molecular Weight DNA extraction kit (Promega) following the plant tissue protocol ...
-
bioRxiv - Cell Biology 2023Quote: ... 0.1 mM luciferin (Promega), antibiotics ...
-
bioRxiv - Immunology 2023Quote: ... 1 Inflammasome Assay was from Promega. HTRF kits to detect IL-1b ...
-
bioRxiv - Cancer Biology 2023Quote: ... or Cell-Titer Glo (Promega) was used to determine the cell number under various treatments ...
-
bioRxiv - Cell Biology 2023Quote: ... 30 μL of CellTiter-Glo reagent solution (Promega, diluted 1:6 in water) was added to each well after 24 h and 72 h of compound treatment ...
-
bioRxiv - Neuroscience 2023Quote: ... 5-30 ng of the original RNA was used to perform qPCR for Dkk3 and Dkk1 using GoTaq qPCR Master Mix (Promega) in a CFX96 Bio-rad system following the manufacturer’s protocol (2 min at 95°C followed by 40 cycles of denaturing at 95°C and annealing/extension at 60°C) ...
-
bioRxiv - Microbiology 2023Quote: ... Reverse Transcription Kit (Promega, USA); IBV nucleic acid amplification fluorescence detection kit (Shanghai Furex Medical Scientific and Technological Development Co. ...
-
bioRxiv - Genomics 2023Quote: ... pGL4.50 (Promega). LTR10 consensus sequences for subfamilies LTR10A and LTR10F were downloaded from Dfam ...
-
bioRxiv - Cell Biology 2023Quote: Cell-based ProteasomeGlo™ chymotrypsin-like and trypsin-like assays (Promega) were performed according to manufacturer’s instructions (Moravec et al ...
-
bioRxiv - Cancer Biology 2023Quote: ... and identity confirmed by short tandem repeat (STR) testing using GenePrint 10 (Promega) (Supplementary Table 12) ...
-
bioRxiv - Genomics 2023Quote: ... at 0.5 uM or DMSO (vehicle) followed by measurement of Caspase activity via the Caspase-Glo 3/7 assay (Promega). Results are representative of at least 3 independent experiments ...
-
bioRxiv - Microbiology 2023Quote: ... Guides targeting 5’ and 3’ regions (see primer sequences in Supplementary File 6) of the PIP5Pase coding sequence were produced by in vitro transcription with T7 polymerase (Promega), and co-transfected with recombinant saCas9 Nuclease NLS Protein (Applied Biological Materials Inc.) ...
-
bioRxiv - Molecular Biology 2023Quote: ... or Maxwell® RSC miRNA from Plasma or Serum (Cat# AS1680, Promega) following the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2023Quote: ... Assays were executed as recommended by Promega, using 0.31 μM of tracer K11 for CDKL5 ...
-
bioRxiv - Cancer Biology 2023Quote: ... and samples were digested with sequencing grade Trypsin/Lys-C enzyme (Promega) in the presence of 10% Acetonitrile for 16 hours at 37°C ...
-
bioRxiv - Biophysics 2023Quote: ... The secondary antibody (Promega: anti-rabbit #W4011 ...
-
bioRxiv - Bioengineering 2023Quote: ... the luciferase expression was assayed using the BrightGlo system (Promega).
-
bioRxiv - Cancer Biology 2023Quote: ... with 150 mg/kg firefly luciferin (Promega) before being transferred to the IVIS Spectrum camera and maintained at 37 °C ...