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Citations for Promega :
9201 - 9250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The P450-Glo CYP1A1/1B1 assay kit and NADPH regeneration system and luciferase assay kit were purchased from Promega (Promega, Madison, WI) and utilized under the following conditions ...
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bioRxiv - Genetics 2023Quote: ... and amplified by PCR using the GoTaq Master Mix (Promega, Charbonnières les Bains, France). We employed specific primers for PTGER1 ...
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bioRxiv - Developmental Biology 2023Quote: ... The prospero 3’UTR [2131bp] was subcloned into the pmirGlo Dual-Luciferase expression vector (Promega) downstream of the firefly luciferase gene ...
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bioRxiv - Developmental Biology 2023Quote: ... cloned into pGEM-T (Promega) and used to synthesize digoxigenin-labeled probes by in vitro transcription using SP6 or T7 polymerases (Thermo-Fisher) ...
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bioRxiv - Genetics 2023Quote: ... The first-strand cDNA from a colonic sensory neuron was used as template in a PCR reaction containing 1×GoTaq reaction buffer (Promega Madison, WI), 20 mM outer primers ...
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bioRxiv - Cell Biology 2023Quote: ... Total RNA was reverse transcribed using oligo(dT) primers and MMLV reverse transcriptase (Promega, PR-M1705) to generate cDNA ...
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bioRxiv - Cell Biology 2023Quote: ... The inserts and plasmid backbones were purified with Promega Wizard SV gel and PCR Cleanup System (Promega). Purified inserts and backbones were mixed in a molar 3:1 ratio ...
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bioRxiv - Genetics 2023Quote: ... 10 µL of CellTiter-Blue Viability reagent (Promega) was added to each well ...
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bioRxiv - Immunology 2023Quote: ... 100 μl of culture medium were discarded and addition with 100 μl of Bright-Glo luciferase reagent (Promega, E6120) in each well ...
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bioRxiv - Immunology 2023Quote: ... and pMD2.G were co-transfected by Fugene HD Transfection Reagent (Promega Corporation, Madison, WI). The supernatant was collected at 48 h ...
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bioRxiv - Immunology 2023Quote: ... The linearized plasmid was then purified using the Wizard Plus SV Miniprep DNA Purification System (Promega), and saRNA was transcribed in vitro using HiScribe T7 High Yield RNA Synthesis Kit (NEB) ...
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bioRxiv - Cell Biology 2023Quote: ... pulsed for 20 min with 100 nM TMR ligand (Promega, G8251), washed twice in 1x DPBS ...
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bioRxiv - Cell Biology 2023Quote: ... in-gel digestion with trypsin (Promega) overnight ...
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bioRxiv - Cell Biology 2023Quote: ... and ImProm II reverse transcriptase (Promega). Quantitative RT-PCR was performed using a Thermal Cycler Dice Real Time System II (Takara Bio Inc. ...
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bioRxiv - Cell Biology 2023Quote: ... The NO concentration in the culture medium was measured using the Griess reagent system (Promega, Tokyo, Japan). To determine the direct effects of mannosidase on the matrix ...
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bioRxiv - Cell Biology 2023Quote: Production of hydrogen peroxide (H2O2) in WT and Alas1 KO brown adipocytes was measured using the ROS-GloTM H2O2 assay kit (Promega #G8820) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The PCR products were cloned in the pGEM T Easy vector system (Promega) according to the manufacturer’s instructions and sequenced (Eurofins Genomics and SourceBioscience) ...
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bioRxiv - Immunology 2023Quote: ... FuGENE HD (Promega, E2311), HALT protease inhibitor cocktail (Thermo Scientific ...
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bioRxiv - Genomics 2023Quote: ... Reference allele enhancer sequences for hZRS and hs737 were obtained via PCR cloning from human genomic DNA (Promega, G304A). Primers used for each enhancer sequence are outlined in Table S2 ...
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bioRxiv - Systems Biology 2023Quote: ... and 500 µL per sample were digested using 2 µg Trypsin/LysC (Promega, Cat#V5072). After 17 h of incubation at 37°C ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from frozen ground tissues using the innuPREP Plant RNA kit (Analytic Jena) and digested with RQ1 DNase (Promega). First-strand cDNA synthesis was conducted on 1 µg RNA using the SuperScript III Reverse Transcriptase (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... with primer dimers being removed using the ProNex Size-Selective Purification System (Promega, NG2001). After a second PCR amplification— 98°C for 30 s (1X) ...
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bioRxiv - Physiology 2023Quote: ... GLP-1 was detected by indirect sandwich amide chemiluminescence ELISA using a luminescence plate reader (GloMax Promega, USA). This total GLP-1 assay detects GLP-1 (7-36 ...
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bioRxiv - Plant Biology 2023Quote: ... and P5Solexa and P3Solexa primers (Supplementary Data 12) on the whole eluted volume and a second ProNEX purification step (Promega, NG2001) to remove residual primers ...
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bioRxiv - Physiology 2023Quote: ... caspase 3/7 activity was measured using Apo-ONE® Homogenous Caspase 3/7 Assay (Promega). VSMC were grown on a 96 well plate and 50 µl of the reagent was added to 50 µl of medium in each well ...
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bioRxiv - Plant Biology 2023Quote: ... and digested overnight at 37° C in 1.5M Urea with modified trypsin (Promega) at a 1:50 enzyme-to-substrate ratio ...
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bioRxiv - Plant Biology 2023Quote: ... (2018) with some modifications: using the ImProm-IITM Reverse Transcriptase (Promega), in which 1 μg of treated RNA was adjusted in a volume of 8.5 µl with RNAse-free water ...
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bioRxiv - Plant Biology 2023Quote: ... Individuals of the F2 progeny were analysed by PCR genotyping using GoTaq2 DNA polymerase (Promega) according to standard protocols ...
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bioRxiv - Plant Biology 2023Quote: ... RNase-Free DNase (Promega, USA) treatment of the isolated RNA samples was done to remove any DNA contamination ...
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bioRxiv - Plant Biology 2023Quote: ... The 16S rDNA PCR-amplified fragments were purified by Wizard® SV Gel and PCR Clean-Up System (Promega, New Delhi, India) and digested by HaeIII (SRL ...
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bioRxiv - Plant Biology 2023Quote: ... Protein marker was purchased from Fermentas (Beijing) and M-MLV reverse transcriptase was purchased from Promega (Beijing). Homologous recombinant kit was purchased from Clone Smarter (Beijing) ...
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bioRxiv - Plant Biology 2023Quote: ... and treated with RNAse-free DNase I (Promega) to remove residual genomic DNA ...
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bioRxiv - Neuroscience 2023Quote: ... we added RNasin Plus (5% of final volume, Promega, Madison, WI). After immunostaining ...
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bioRxiv - Neuroscience 2023Quote: ... 1.8 mg pAdvantage (Promega; #E1711) and 2.4 mL P3000 reagent diluted in 80 mL Opti-MEM I reduced serum medium (ThermoFisher #31985070) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 0.5 µg (HEK293A, HEK293T, HEK293AΔARRB1/B2) or 2 µg (HEK293AΔGs, HEKS293AΔGi) Glo-22F cAMP plasmid DNA (Promega) and 2 µg MOCK DNA (HEK293A ...
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bioRxiv - Neuroscience 2023Quote: ... and the cell viability was determined using CellTiter-Glo viability assay kit (Promega) at different time points according to the manufacturer’s protocols ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... membrane integrity (CytoTox-OneTM homogeneous membrane integrity assay kit: lactate dehydrogenase (LDH release) and caspase activity/ apoptosis (Caspase-Glo® 3/7 assay system kit) (all from Promega, WI, USA), as described previously17 (see Supplementary data for detailed description).
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bioRxiv - Physiology 2023Quote: ... solid-phase-enhanced sample preparation with sequencing grade modified porcine trypsin (Promega) 39 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: A Human Embryonic Kidney 293 cell line (HEK 293; ATCC CRL-1573) stably transfected with luciferase-based pGlosensor-22F cAMP reporter plasmid (Glosensor, Promega Corp) has been described previously [GS2257] ...
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bioRxiv - Neuroscience 2023Quote: 400 µL of HaloLinkTM resin (Promega, G1912) was washed three times with Halo binding buffer (1x PBS with 0.005% IGEPAL® CA-630 and 0.5mM TCEP) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... CP4 medium was exchanged with Hanks’Balanced Salt Solution (with added glucose 1 g/l, NaHCO3 0.35 g/l) containing GloSensor cAMP Reagent (3 %, Promega) and plates were incubated for 60 min ...
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bioRxiv - Neuroscience 2023Quote: ... using a dounce homogenizer and incubated for 15 minutes on ice and treated with RQ1 DNase (Promega) for 5 min at 37 °C ...
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bioRxiv - Plant Biology 2023Quote: ... Plasmid purification from 50 mL bacterial cultures was made using PureYield™ Plasmid Midiprep System (Promega, A2492) for C ...
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bioRxiv - Neuroscience 2023Quote: ... Trypsin (Promega, Cat# V5280); S-TrapTM mini-MS sample prep kit (Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: Cells were harvested to extract RNA using ReliaPrep™ RNA Cell Miniprep System (Promega). CDNA was reverse-transcribed using PrimeScript™ RT reagent Kit (Takara) ...
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bioRxiv - Neuroscience 2023Quote: ... using a dounce homogenizer and incubated for 15 minutes on ice and treated with RQ1 DNase (Promega) for 5 min at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... coli and isolated via mini-preps (Promega, A1330). Plasmid stocks for each GPCR had a 260/280 ratio of 1.7–1.9 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Samples were converted into cDNA by incubation with M-MLV Reverse Transcriptase (Promega) and dNTP Mix (Promega) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... wells were filled with 20 µl of Bright-Glo reagent per well after discarding the medium and drug solutions (Promega). After 20 minutes of dark incubation ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 25uL of coelenterazine h (Promega) was added to each well to bring the final concentration to 5 uM ...