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Citations for Promega :
9101 - 9150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The samples were digested overnight at 37°C with sequencing-grade modified trypsin (Promega) at an enzyme to protein ratio of 1:40 ...
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bioRxiv - Microbiology 2023Quote: The quantification of viral RNA was carried out in one-step RT-qPCR using 3 µl of the eluted RNA with the GoTaq® Probe One-step RT-qPCR System (Promega, Poland). This procedure employed in-house primers and a probe specifically tailored for the HCoV-229E N sequence ...
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bioRxiv - Microbiology 2023Quote: ... and extracted using the PureYield Plasmid Miniprep System (Promega, Madison, WI, USA, Cat# A1222). The sequence of the plasmid was verified by sequencing using a SupreDye v3.1 Cycle Sequencing Kit (M&S TechnoSystems ...
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bioRxiv - Microbiology 2023Quote: ... The sequence of the plasmid was verified by sequencing using a SupreDye v3.1 Cycle Sequencing Kit (M&S TechnoSystems, Osaka, Japan, Cat# 063001) with a Spectrum Compact CE System (Promega). To generate a pDON-5 Neo-vector expressing IFNAR1 with the W70C mutation ...
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bioRxiv - Microbiology 2023Quote: ... using FuGene®6 (Promega) according to the manufacturer’s instructions (Fodor et al. ...
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bioRxiv - Microbiology 2023Quote: ... The RNA amount in the eluted fraction was quantified with Quanti Fluor RNA dye (E286A, Promega, Madison, WI, USA) by following the manufacturer’s instruction ...
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bioRxiv - Genetics 2023Quote: ... The pGL4.23 constructs were co-transfected with a renilla internal control vector pRL-TK (Promega, Madison, Wisconsin, United States) in a 50:1 ratio in SH-SY5Y cells in 6-well plates with a seeding density of 1 million cells per well ...
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bioRxiv - Immunology 2023Quote: ... and 20 µL of CellTiter 96 (Promega, Tokyo, Japan) was added and incubated at 37°C and 5% CO2 for 2 hours ...
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bioRxiv - Immunology 2023Quote: ... and REN-Luc/pRL-TK plasmid (20 ng; Promega) expressing Renilla luciferase used as an internal control to normalize transfection efficiency ...
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bioRxiv - Immunology 2023Quote: ... Cells (1.5×104/well) were subjected to a GSH/GSSG luminescence-based assay (Promega) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... and cell lysates were examined for luciferase activities with the luciferase assay system kit (Promega). The relative luciferase activities were calculated after normalization with the ß-gal activities ...
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bioRxiv - Cancer Biology 2023Quote: Cell viability was determined using CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega), utilising MTS reagent ...
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bioRxiv - Cancer Biology 2023Quote: ... CellTiter 96 MTS was used to estimate cell numbers according to the manufacturer’s instructions (Promega). Each assay was performed in triplicate with three biological replicates ...
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bioRxiv - Biochemistry 2023Quote: ... 10 μg of the isolated recombinant bacmid and 5 μL Fugene HD transfection reagent (Promega) were diluted in 150 μL Sf-900™ III SFM ...
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bioRxiv - Biochemistry 2023Quote: ... The measurements used the Enliten ATP Assay System (Bioluminescence Detection Kit, Promega, Madison, WI). The luminescence reaction contained 5 µL of neutralized acid-soluble supernatant and 45 µL of luciferin/luciferase reagent ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... The sequence was than cloned into pGem-T Easy (Promega, cat# A1360) while restriction enzyme binding sites were inserted to further clone the construct into the LigAF vector (for sequences see suppl ...
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bioRxiv - Biochemistry 2023Quote: ... RNA (3 μg) was retro-transcribed by using M-MLV (Promega, Madison, WI). qPCR was performed in triplicate by using validated qPCR primers (BLAST) ...
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bioRxiv - Biochemistry 2023Quote: ... GAG/POL and VSVG plasmids were co-transfected with transfer plasmid into HEK293Ts or HEK293FTs using FuGENE HD (Promega, 3.33:1 FuGENE:DNA), or lipofectamine 3000 Reagent (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Random Primers (Promega, Madison, WI, USA) following the manufacturer protocol ...
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bioRxiv - Microbiology 2023Quote: ... using the Bright-Glo Luciferase Assay System (Promega). Percent inhibition of pseudovirus infection was calculated relative to pseudovirus-only control ...
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bioRxiv - Microbiology 2023Quote: ... CellTiter-Blue (Promega, G8080) reagent was added to each well at a 1:10 ratio ...
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bioRxiv - Molecular Biology 2023Quote: ... Colorimetric detection was performed using an NBT/BCIP detection kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The pellet was then suspended in 1.5 mL Fast-Break lysis buffer (Promega, Madison, WI) with 1 mM PMSF ...
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bioRxiv - Molecular Biology 2023Quote: ... on-column trypsin digestion was performed by adding by mass spectrometry grade trypsin/Lys-C mix (Promega) in a 50 mM NH4HCO3 buffer overnight at 37 °C.
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bioRxiv - Molecular Biology 2023Quote: ... The Luciferase assay was performed with 100 µL of the cleared lysate (Steady Glo reagent, Promega, E2510) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The captured proteins were washed twice in 200 μl of 95% MeCN and transferred to 200 μl of 50 mM ammonium bicarbonate (ABC) containing 1 μg sequencing grade modified trypsin (Promega, Madison, USA) and mixed for 2 hr at 37 °C ...
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MET functions in tumour progression and therapy resistance are repressed by intronic polyadenylationbioRxiv - Molecular Biology 2023Quote: ... Luciferin (K salt, Promega) was injected intraperitoneally at a dose of 150 mg/kg of body weight 15 minutes before imaging ...
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MET functions in tumour progression and therapy resistance are repressed by intronic polyadenylationbioRxiv - Molecular Biology 2023Quote: ... PCR reactions were performed using 10 ng of cDNAs (RNA equivalent) and the GoTaq DNA polymerase (Promega, # M8295). For each gene ...
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bioRxiv - Neuroscience 2023Quote: ... urea concentration for each sample was diluted to <1 M using ammonium bicarbonate (ABC) and digested for an additional ∼16 hours using 1:50 (w/w) trypsin (Promega). Following digestion ...
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bioRxiv - Neuroscience 2023Quote: ... UDP (at concentrations ranging from 1 nM to 1 mM) was then applied with a final concentration of 5 μM furimazine (NanoLuc Luciferase Assay, Promega). Luminescence was subsequently measured using a Victor X5 multi-label plate reader (PerkinElmer).
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bioRxiv - Neuroscience 2023Quote: ... The PCR reaction mixture for all reactions consisted of 2x GoTaq Green Mastermix (Promega), 400nM primer-F ...
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bioRxiv - Biochemistry 2023Quote: ... measurement of nitrite concentration in the media using Griess Reagent System (Promega, G2930), and measurement of intracellular citrulline abundance by LCMS.
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bioRxiv - Molecular Biology 2023Quote: ... Viability was quantified 48 hours after treatment using a luminescent system (CellTiter-Glo, Promega). For clonogenic assays ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were used for luciferase assays (20 µL) with Luciferase assay system (Promega) and RNA extraction (20 µL ...
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bioRxiv - Microbiology 2023Quote: ... The 16S rRNA fragment was amplified using 0.625 units of GoTaq DNA Polymerase (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... cell viability was measured using CellTiter Glo (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and firefly luciferase activity was measured with the Dual-Glo Luciferase Assay system (Promega) and normalized to that of Renilla luciferase.
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bioRxiv - Biochemistry 2023Quote: ... by transfection of the pSpCas9(BB)-2A-Puro (PX459) plasmid containing A3B gRNAs with FuGENE 6 Transfection Reagent (E2691; Promega). 16h after transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA for PCR was isolated from 50 µl of tissue lysate using the Maxwell RSC tissue DNA Kit (Promega, Madison, WI, USA).
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bioRxiv - Molecular Biology 2023Quote: ... RNA was purified using the Maxwell RSC simply RNA tissue extraction kit (Promega) with on-bead DNase I digestion ...
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bioRxiv - Neuroscience 2023Quote: Apoptotic cells were detected using the DeadEnd™ Fluorometric TUNEL System (Promega, G3250, USA) following the manufacturer′s protocol.
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bioRxiv - Molecular Biology 2023Quote: ... DNA was purified with ProNex size-purification system (NG2001, Promega) with 8:5 beads to sample ratio ...
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bioRxiv - Cell Biology 2023Quote: ... 10mM Ultrapure ATP and 0.7 μg of recombinant full-length human CDC7/DBF4 kinase (Promega, V5088). Reactions were incubated at 37 °C for 120 min and then terminated by freezing on dry ice before mass spectrometry.
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bioRxiv - Cell Biology 2023Quote: ... using the rabbit reticulocytes lysates Nuclease-treated kit and the transcendTM tRNA from Promega, as per the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: ... the samples were directly spiked with 50 pg of unmethylated lambda phage DNA (Promega), followed by bisulfite treatment and library construction as described for the FGO libraries ...
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bioRxiv - Cancer Biology 2023Quote: ... regions of mtDNA were cloned from donor cell lines into p-GEM-T Easy vector (Promega, A1360). Samples were then diluted to specific concentrations and analyzed to evaluate probe specificity.
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bioRxiv - Cell Biology 2023Quote: ... Samples were directly spiked with 5 pg of unmethylated lambda phage DNA (Promega) and subjected to bisulfite treatment and library construction using the post-bisulfite adaptor tagging (PBAT ...
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bioRxiv - Molecular Biology 2023Quote: ... The Nano-Glo® Live Cell Assay System was purchased from Promega and used as received (Cat #N2012) ...
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bioRxiv - Cell Biology 2023Quote: ... The bacmid was transfected into Sf9 cells using Fugene HD transfection reagent (Promega). The obtained viral stock was amplified and used for protein expression ...
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bioRxiv - Cell Biology 2023Quote: HeLa cells were transfected using 2 µg of plasmid DNA and 6 µL of FuGENE (Promega) for gene editing or using a NEPA21 electroporation system (Nepa Gene ...